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Antiproliferative effect of silver nanoparticles synthesized using amla on Hep2 cell line

Asian Pacific Journal of Tropical Medicine 2013年第1期6卷 1-10页

作者： Fathima Stanley Rosarin Vadivel Arulmozhi Samuthira Nagarajan sankaran mirunalini Department of Biochemistry and Biotechnology Annamalai UniversityAnnamalai nagar-608 002TamilnaduIndia Department of Chemistry Annamalai UniversityAnnamalai nagar-608 002.TamilnaduIndia

Objective:To synthesize silver nanoparticles by amla extract,screen the cytotoxic,oxidative stress and apoptotic effect of silver nanoparticles（AgNPs） on Hep2 cell line（laryngeal carcinoma cells） in vitro,and to compare the effect of Phyllanthm emblica（***）（amla） with AgNPs synthesized by amla and ***:AgNPs was synthesized by ***（aqueous extract） and nanoparticles were characterized UV-Vis spec,the presence of biomoloecules of amla capped in AgNPs was found by FT-IR analysis,shape and size were examined by SEM and *** of experimental drugs was tested to find IC50 *** generation in cells have been measured by DCFH-DA ***-EtBr,Rhodamine-123 staining and DNA fragmentation were performed to assess apoptotic cell death,mitochondrial membrane potential and apoptotic DNA damage,*** stress was analyzed by measuring lipid peroxides and antioxidants level lo understand the cancer cell death by pro-oxidant mechanism. Results:PE-AgNPs was synthesized and confirmed through kinetic behavior of *** shape of PE-AgNPs was spherical and cubic since it was agglomerated,and the nanoparticle surface was *** particle size distribution of PE-AgNPs was found to be 188 *** biomolecules of *** such as polyphenols were capped with AgNPs and reduced its toxicity. In cytotoxicity assay the concentration in which the maximum number of cell death was 60μg/mL and 50μg/mL for ***（alone） and AgNPs,respectively and IC50 values were fixed as 30μg/mL and 20μg/*** generation,apoptotic morphological changes,mitochondrial *** damage and oxidative stress was observed as more in AgNPs treated cells than in ***（30μg/mL）（alone） treated cells and 5-FU treated cells gave similar result. Conclusions:The results suggest that the AgNPs are capped with biomolecules of amla enhanced cytotoxicity in laryngeal cancer cells through oxidative stress and apoptotic function

关键词： Amla AgNPs Oxidative stress Cytotoxicity Antiproliferation

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Quantitative variation of bioactive phyto compounds in ethyl acetate and methanol extracts of Pergularia daemia(Forsk.)Chiov.

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The Journal of Biomedical Research 2015年第2期29卷 169-172页

作者： Veluchamy Vaithiyanathan sankaran mirunalini Department of Biochemistry and Biotechnology Annamalai University Annamalainagar Chidambaram- 608002 India . Tel: 09442-424-438 E-mail: mirunasankar@*** .

Dear Editor： Pergularia daemia Forsk （Asclepiadaceae） is a perennial twining herb commonly known as veliparuthi in Tamil. The plant has anthelmintic, laxative, antidiabetic, hepatoprotective and anti-inflammatory activities. The pharmacological properties of this plant come from bioactive phytochemicals such as alkaloids, triterpenes, saponins and flavonoids. Phytochemically, the plant has been investigated for the presence of cardenolides, alkaloids, saponins and steroidal compoundst. In the present study, we developed a rapid method for identification and quantitative determination of putative phyto compounds in the crude extracts of ethyl acetate and methanol from whole plant of Pergularia daemia.

关键词： Forsk Quantitative variation of bioactive phyto compounds in ethyl acetate and methanol extracts of Pergularia daemia Chiov

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