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Effects of PKCa antisense RNA on human breast cancer cell proliferation and expression of cyclinD1 and CDK4

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Chinese Science Bulletin 1999年第12期44卷 1110-1114页

作者： sang jianli wang yongchaolaboratory of cell biology , department of biology, beijing normal university, beijing 100875 , china Laboratory of cell biology Department of Biology Beijing Normal University Beijing China

The role of PKCα in human breast cancer cell proliferation and expression of cyclinD1 and CDK4 has been investigated using inhibition of PKCα expression by its antisense RNA. When PKCα expression was inhibited the rate of cell proliferation decreased apparently and the levels of cyclinD1 and CDK4 mRNA were lower than the control. The results showed that PKCα, a key member of signal transduction system, played an important role in human breast cancer cell proliferation and had a close relationship with expression of cyclinD1 and CDK4 which control start of cell cycle.

关键词： breast cancer PKCα cell proliferation cyclinD1 CDK4.

Effects of enhancing p21^(waf1) on proliferation and expression of G1 cyclins and CDKs in human breast cancer cells

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Chinese Science Bulletin 1999年第16期44卷 1489-1493页

作者： sang jianli, wang Yongchao and YE Yingjiang1. Laboratory of cell biology , department of biology , beijing normal university , beijing 100875 , china 2. The Second Hospital, beijing Medical university, beijing 100044, china Laboratory of cell biology Department of Biology Beging Normal University Beijing China The Second Hospital Beijing Medical University Beijing China

The cyclin-dependent kinase inhibitor p21( waf1/cip1/sdi1) is an important negative regulator in control of cell cycle. Its functions of inhibiting cancer cell growth and its effects on expression of Gl phase cyclins and related CDKs are a worthy topic for study. The plasmid expressing p2l with high level was transformed to human breast cancer cells, and the expression of p2l in cells was enhanced, then the cell growth rate, anchorage-independent growth and tu-morigenecity were tested, at the same time the expression levels of cyclinD1, CDK4,

关键词： p21waf1 breast cancer cell cyclin CDK.

C-terminal domain of Chk1 regulates its subcellular location and kinase activity for DNA repair

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Chinese Science Bulletin 2011年第30期56卷 3138-3147页

作者： NING LiFeng wang HuiPing SAN Dan sang jianli Key Laboratory for cell Proliferation and Regulation biology of Ministry of Education College of Life Science Beijing Normal University Beijing 100875 China department of Reproduction Immunology National Research Institute for Family Planning Beifing 100081 China

Effector kinase Chk1 is an evolutionarily conserved protein kinase. It is a key mediator linking the mechanisms that monitor DNA integrity to components of the cell cycle engine. In this study, recombinant vectors pEGFP-C1-Chk1/C 288/C 334/C 368 were constructed and transfected into HeLa cells to study the effect of the Chk1 regulatory domain on the regulation of subcellular Chk1 location in response to DNA damage. We found that DNA damage-induced nuclear accumulation is regulated by 34 amino acids (334–368) in the C-terminal regulatory domain. Recombinant vectors pXJ41-Chk1/C 288/C 334/C 368 were co-transfected with reporter plasmid pEGFP-N2 into HeLa cells to study the repair abilities of the different human Chk1 truncation mutants. In addition, recombinant vectors were transfected into HeLa cells to study the effects of the different truncation mutants on the cell cycle. Furthermore, to study the kinase activity of the different truncation mutants, Ser216 phosphorylation of Cdc25C was studied by Western blot analysis. We found that the enzymatic activity of C 368, missing the 108 C-terminal amino acids (368–476), was higher than that of full-length Chk1, and C 368 delayed the cell cycle progression. The enzymatic activity of C 334, missing the 142 C-terminal amino acids (334–476), was equivalent to that of full-length Chk1. C 288, missing the 188 C-terminal amino acids (288–476), had almost no enzymatic activity, suggesting that the regulatory domain contains both inhibitory and regulatory elements. This study provides useful information for further research on Chk1 function.

关键词：亚细胞定位激酶活性 DNA修复 HeLa细胞 DNA损伤细胞周期重组载体 blot分析

CaSfl1 plays a dual role in transcriptional regulation in Candida albicans

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Chinese Science Bulletin 2008年第17期53卷 2624-2631页

作者： ZHANG TingTing LI Di LI WanJie wang Yue sang jianli Key Laboratory of cell Proliferation and Regulation of Ministry of Education Beijing Normal University Beijing 100875 China Candida albicans Molecular and cell biology Laboratory Institute of Molecular and Cell Biology Proteos 61 Biopolis Drive Singapore 138673 Singapore

As a newly identified transcription factor in Candida albcians, CaSfl1 has been shown to be involved in cell flocculation and filamentation and in the negative regulation of several genes involved in hyphal growth. In this study, we constructed Casfl1△/△ mutants and confirmed that deletion of this gene indeed affected cell flocculation and filamentation. In addition, by RT-PCR we found that while Casfl1 repressed the expression of several hypha-specific genes including HWP1, ECE1, ALS1, ALS3, and FLO8, it strongly activated the expression of the heat-shock protein genes HSP30 and HSP90 under certain stress conditions. Therefore, we propose that CaSfl1 can act as both positive and negative regulators, thereby playing a dual role in transcriptional controls in Candida albicans.

关键词：假丝酵母 CaSFL1 基因注入形态发生转录规则

Role of Ppt1 in multiple stress responses in Candida albicans

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Chinese Science Bulletin 2014年第31期59卷 4060-4068页

作者： Kangdi Hu Wanjie Li Jiaxin Gao Qizheng Liu Haitao wang Yue wang jianli sang Key Laboratory of cell Proliferation and Regulation biology Ministry of EducationCollege of Life ScienceBeijing Normal University School of Biotechnology and Food Engineering Hefei University of Technology Institute of Molecular and cell biology Agency for ScienceTechnology and Research (A*STAR)

To study the function of CaPpt1,we deleted PPT1 gene from the Candida albicans genome by sequentially replacing the entire coding region with the selectable markers ARG4 and *** results showed that the deletion of Ppt1 did not affect the hyphal formation of *** under serum induction and caused enhanced sensitivity to DNA damage,Calcofluor white and saltinduced *** also found that Ppt1 was not required for the phenotypic response of cells treated with the genotoxins,methylmethane sulfonate and *** cytometric analyses indicated that ppt1D cells and wild-type cells showed similar G2/M arrest profiles when exposed to DNA damage ***1 was not required for the activation of the DNA damage response pathway,as indicated by normal phosphorylation of Rad53 and Rfa2 in ppt1D cells under DNA damage *** suggest that Ppt1 plays important roles in response to various stress conditions in ***.

关键词：白色念珠菌应激反应流式细胞分析 DNA损伤选择性标记基因组胁迫条件编码区

Tpd3-Pph21 phosphatase plays a direct role in Sep7 dephosphorylation in Candida albicans

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Tpd3-Pph21 phosphatase plays a direct role in Sep7 dephospho...