检索结果-南通市图书馆

Endoscopic and clinicopathologic characteristics of early gastric cancer with high microsatellite instability

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2012年第27期18卷 3571-3577页

作者： Jaehoon Jahng Young Hoon Youn Kwang hyun Kim Junghwan Yu Yong Chan Lee Woo jin hyung Sung Hoon Noh hyunki Kim Hoguen Kim Hyojin Park sang In Lee Department of Internal Medicine Gangnam Severance HospitalYonsei University College of MedicineSeoul 135-720South Korea Department of Internal Medicine Institute of GastroenterologyYonsei University College of MedicineSeoul 100-753South Korea Department of Surgery Yonsei University College of MedicineSeoul 100-753South Korea Department of Pathology Yonsei University College of MedicineSeoul 100-753South Korea

AIM： To investigate endoscopic and clinicopathologic characteristics of early gastric cancer （EGC） according to microsatellite instability phenotype. METHODS： Data were retrospectively collected from a single tertiary referral center. Of 981 EGC patients surgically treated between December 2003 and October 2007, 73 consecutive EGC patients with two or more microsatellite instability （MSI） mutation [high MSI （MSI-H）] and 146 consecutive EGC patients with one or no MSI mutation （non-MSI-H） were selected. The endoscopic and clinicopathologic features were compared between the MSI-H and non-MSI-H EGC ***： In terms of endoscopic characteristics, MSI-H EGCs more frequently presented with elevated pattern （OR 4.38, 95% Cl： 2.40-8.01, P 〈 0.001）, moderate- to-severe atrophy in the surrounding mucosa （OR 1.91, 95% CI： 1.05-3.47, P = 0.033）, antral location （OR 3.99, 95% CI： 2.12-7.52, P 〈 0.001） and synchronous le- sions, compared to non-MSI-H EGCs （OR 2.65, 95% CI： 1.16-6.07, P = 0.021）. Other significant clinicopatholog- ic characteristics of MSI-H EGC included predominance of female sex （OR 2.77, 95% CI： 1.53-4.99, P 〈 0.001）, older age （〉 70 years）（OR 3.30, 95% CI： 1.57-6.92, P = 0.002）, better histologic differentiation （OR 2.35, 95% Cl： 1.27-4.34, P = 0.007）, intestinal type by Lau- ren classification （OR 2.34, 95% CI： 1.15-4.76, P = 0.019）, absence of a signet ring cell component （OR 2.44, 95% CI： 1.02-5.86, P = 0.046）, presence of mu- cinous component （OR 5.06, 95% Cl： 1.27-20.17, P = 0.022）, moderate-to-severe lymphoid stromal reaction （OR 3.95, 95% CI： 1.59-9.80, P = 0.003）, and co-exist- ing underlying adenoma （OR 2.66, 95% CI： 1.43-4.95, P = 0.002）. CONCLUSION： MSI-H EGC is associated with unique endoscopic and clinicopathologic characteristics includ- ing frequent presentation in protruded type, co-exist- ing underlying adenoma, and synchronous lesions.

关键词： Microsatellite instability Early gastric can-cer Endoscopic characteristic Advanced gastric cancer

Induction of clusterin Expression by Neuronal Cell Death in Zebrafish

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2014年第11期41卷 583-589页

作者： Yun-Mi Jeong Tae-Eun jin Jung-Hwa Choi Mi-Sun Lee hyun-Taek Kim Kyu-Seok Hwang Doo-sang Park hyun-Woo Oh Joong-Kook Choi Vladimir Korzh Melitta Schachner Kwan-Hee You Cheol-Hee Kim Department of Biology Chungnam National University Korea Research Institute of Bioscience and Biotechnology Department of Biochemistry College of MedicineChungbuk National University Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research Keck Center for Collaborative Neuroscience and Department of Cell Biology and Neuroscience Rutgers University Center for Neuroscience Shantou University Medical College

Clusterin, a protein associated with multiple functions, is expressed in a wide variety of mammalian tissues. Although clusterin is known to be involved in neurodegenerative diseases, ageing, and tumorigenesis, a detailed analysis of the consequences of gain- or loss-of- function approaches has yet to be performed to understand the underlying mechanisms of clusterin functions. Since clusterin levels change in neurological diseases, it is likely that clusterin contributes to cell death and degeneration in general. Zebrafish was investigated as a model system to study human diseases. During development, zebrafish clusterin was expressed in the notochord and nervous system. Embryonic overexpression of clusterin by mRNA microinjection did not affect axis formation, whereas its knock-down by anti-sense morpholino treatment resulted in neuronal cell death. To analyze the function of clusterin in neurodegeneration, a transgenic zebrafish was investigated, in which nitroreductase expression is regulated under the control of a neuron-specifc huC promoter which is active between the stages of early neuronal precursors and mature neurons. Nitroreductase turns metronidazole into a cytotoxic agent that induces cell death within 12 h. After metronidazole treatment, transgenic zebrafish showed neuron-specific cell death. Interestingly, we also observed a dramatic induction of clusterin expression in the brain and spinal cord in these fish, suggesting a direct or indirect role of clusterin in neuronal cell death and thus, more generally, in neurodegeneration.

关键词： Clusterin Neuronal cell death Neurodegeneration Zebrafish

Clobenpropit enhances anti-tumor effect of gemcitabine in pancreatic cancer

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2014年第26期20卷 8545-8557页

作者： Woo hyun Paik Ji Kon Ryu Kyoung-Sin Jeong jin Myung Park Byeong Jun Song sang Hyub Lee Yong-Tae Kim Yong Bum Yoon Department of Internal Medicine Inje University Ilsan Paik Hospital Division of Gastroenterology Department of Internal Medicine and Liver Research InstituteSeoul National University College of Medicine

AIM: To evaluate the anti-tumor effect of clobenpropit, which is a specific H3 antagonist and H4 agonist, in combination with gemcitabine in a pancreatic cancer cell line.

关键词： Clobenpropit Epithelial-mesenchymal transition Histamine Histamine receptors Pancreatic neoplasm

Submergence promotes auxin-induced callus formation through ethylene-mediated post-transcriptional control of auxin receptors

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2022年第12期15卷 1947-1961页

作者： Seung Yong Shin Yuri Choi sang-Gyu Kim Su-jin Park Ji-Sun Park Ki-Beom Moon hyun-Soon Kim Jae Heung Jeon Hye Sun Cho Hyo-Jun Lee Plant Systems Engineering Research Center Korea Research Institute of Bioscience and BiotechnologyDaejeon 34141Korea Department of Functional Genomics KRIBB School of BioscienceUniversity of Science and TechnologyDaejeon 34113Korea Department of Biological Sciences Korea Advanced Institute of Science and TechnologyDaejeon 34141Korea Department of Biosystems and Bioengineering KRIBB School of BiotechnologyUniversity of Science and TechnologyDaejeon 34113Korea Department of Biological Sciences Sungkyunkwan UniversitySuwon 16419Korea

Plant cells in damaged tissue can be reprogrammed to acquire pluripotency and induce callus ***,in the aboveground organs of many species,somatic cells that are distal to the wound site become less sensitive to auxin-induced callus formation,suggesting the existence of repressive regulatory mechanisms that are largely *** we reveal that submergence-induced ethylene signals promote callus formation by releasing post-transcriptional silencing of auxin receptor transcripts in non-wounded *** determined that short-term submergence of intact seedlings induces auxin-mediated cell dedifferentiation across the entirety of Arabidopsis thaliana *** constitutive triple response 1-1(ctr1-1)mutation induced callus formation in explants without submergence,suggesting that ethylene facilitates cell *** show that ETHYLENE-INSENSITIVE 2(EIN2)post-transcriptionally regulates the abundance of transcripts for auxin receptor genes by facilitating microRNA393 ***-induced calli in non-wounded regions were suitable for shoot regeneration,similar to those near the wound *** also observed submergence-promoted callus formation in Chinese cabbage(Brassica rapa),indicating that this may be a conserved mechanism in other *** study identifies previously unknown regulatory mechanisms by which ethylene promotes cell dedifferentiation and provides a new approach for boosting callus induction efficiency in shoot explants.

关键词： cell dedifferentiation auxin signaling ethylene callus formation EIN2

Midazolam dose is associated with recurrence of paradoxical reactions during endoscopy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2021年第29期9卷 8763-8772页

作者： Eun Hyo jin Ji hyun Song Jooyoung Lee Jung Ho Bae Su jin Chung Department of Internal Medicine Healthcare Research InstituteHealthcare System Gangnam CenterSeoul National University HospitalSeoul 06236South Korea

BACKGROUND Midazolam is commonly used for sedation during gastrointestinal ***,some patients experience paradoxical reactions characterized by excessive movement or *** To investigate the rate of recurrence of paradoxical reactions to midazolam during an upper *** We retrospectively reviewed 122152 sedative endoscopies among a total of 58553 patients at the Seoul National University Hospital,Healthcare System Gangnam Center,from July 2013 to December *** them,361 patients with a history of paradoxical reaction during sedative upper endoscopy were *** characteristics of patients in the recurrent and non-recurrent groups were compared via multivariable analysis using logistic *** Paradoxical reactions occurred in 0.86%(1054/122152)of endoscopies,and in 1.51%(888/58553)of *** the 361 subjects with previous paradoxical reactions in sedative endoscopies,111(30.7%)experienced further paradoxical *** analysis revealed that the total midazolam dose used was higher in the recurrent group(6.74±2.58 mg)than in the non-recurrent group(5.49±2.04 mg;P<0.0001).Patients were administered a lower dose of midazolam than previous doses:1 mg less in the recurrent group and 2 mg less in the non-recurrent *** analysis showed that the midazolam dose difference was an independent risk factor for recurrent paradoxical reaction(odds ratio:1.213,95%CI:1.099-1.338,P=0.0001).CONCLUSION The rate of recurrence of paradoxical reactions is significantly associated with midazolam *** dose of midazolam administered to patients with previous paradoxical reactions should be less than that previously used.

关键词： Gastroscopy Endoscopy Conscious sedation Midazolam Flumazenil

Younger patients have poorer biochemical outcome after radical prostatectomy in high-risk prostate cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2011年第5期13卷 719-723页

作者： Sung Kyu Hong Jung Soo Nam Woong Na Jong jin Oh Cheol Yong Yoon Chang Wook Jeong hyun June Kim Seok-Soo Byun sang Eun Lee Department of Urology Seoul National University Bundang Hospital Seongnam Kyunggi-do 463-707 Korea

The aim of this study was to investigate the prognostic significance of patient age with respect to tumour aggressiveness in men who underwent radical prostatectomy （RP） for prostate cancer. In this study, we reviewed the records of 743 patients who received RP without neoadjuvant or adjuvant therapy at our institution and were followed up for 〉2 years postoperatively. For our analyses, the patients were divided into two groups according to age： younger （〈60 years） and older （≥ 60 years）. Through uni- and multivariate analyses, associations of various clinicopathological parameters, including biochemical recurrence-free survival, with patient age, were evaluated among all patients, and the patients were stratified according to their D＇Amico risk classification. Among all subjects, younger （n= 126） and older （n=617） patients showed no significant differences regarding pathological parameters and biochemical recurrence-free survival （P=0.288）. For the high-risk group （n= 206）, younger patients had a lower rate of biochemical recurrence-free survival following surgery than older patients （P=0.017）, despite the fact that no significant differences were observed regarding various known prognostic parameters between the two age groups. In addition, multivariate analysis revealed that age was an independent predictor of biochemical recurrence-free survival among the high-risk group （P=O.O03）. Our results showed that relatively younger patients have a comparable biochemical outcome compared with their older counterparts following RP performed for prostate cancer. However, among patients with high-risk disease, younger patients have a worse biochemical outcome following RP compared with older patients.

关键词： age factors biochemical outcome prostate prostatic neoplasms prostatectomy

Effects of oxygen concentration and flow rate on cognitive ability and physiological responses in the elderly

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2013年第3期8卷 264-269页

作者： hyun-Jun Kim hyun-Kyung Park Dae-Woon Lim Mi-hyun Choi hyun-Joo Kim In-Hwa Lee hyung-Sik Kim jin-Seung Choi Gye-Rae Tack Soon-Cheol Chung Department of Obstetrics & Gynecology Konkuk University Department of Laboratory Medicine Seoul Clinical Laboratories Department of Information & Communication Engineering Dongguk University Department of Biomedical Engineering Research Institute of Biomedical EngineeringCollege of Biomedical & Health ScienceKonkuk University

The supply of highly concentrated oxygen positively affects cognitive processing in normal young adults. However, there have been few reports on changes in cognitive ability in elderly subjects following highly concentrated oxygen administration. This study investigated changes in cognitive ability, blood oxygen saturation （%）, and heart rate （beats/min） in normal elderly subjects at three different levels of oxygen [21% （1 L/min）, 93% （1 L/min）, and 93% （5 L/min）] administered during a 1-back task. Eight elderly male （75.3 ＋ 4.3 years old） and 10 female （71.1 ＋ 3.9 years old） subjects, who were normal in cognitive ability as shown by a score of more than 24 points in the Mini-Mental State Examination-Korea, participated in the experiment. The experiment consisted of an adaptation phase after the start of oxygen administration （3 minutes）, a control phase to obtain stable baseline measurements of heart rate and blood oxygen saturation before the task （2 minutes） and a task phase during which the 1-back task was performed （2 minutes）. Three levels of oxygen were administered throughout the three phases （7 minutes）. Blood oxygen saturation and heart rate were measured during each phase. Our results show that blood oxygen saturation increased, heart rate decreased, and response time in the 1-back task decreased as the concentration and amount of administered oxygen increased. This shows that administration of sufficient oxygen for optimal cognitive functioning increases blood oxygen saturation and decreases heart rate.

关键词： neural regeneration clinical practice highly concentrated oxygen cognitive task 1-back task cognitive ability blood oxygen saturation heart rate physiological responses elderly grant-supported paper neuroregeneration

Inhibitory activity of gold and silica nanospheres to vascular endothelial growth factor （VEGF）-mediated angiogenesis is determined by their sizes

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nano Research 2014年第6期7卷 844-852页

作者： Dong hyun Jo jin Hyoung Kim jin Gyeong Son Yuanze Piao Tae Geol Lee Jeong Hun Kim Fight against Angiogenesis-Related Blindness (FARB) Laboratory Clinical Research Institute Seoul National University Seoul 110-744 Republic of Korea Department of Biomedical Sciences College of Medicine Seoul National University Seoul 110-799 Republic of Korea Center for Nano-Bio Convergence World Class Laboratory Korea Research Institute of Standards and Science Daejeon 305-340 Republic of Korea Department of Chemistry and KI for the NanoCentury KAIST Daejeon 305-701 Republic of Korea Graduate School of Convergence Science and Technology Seoul National University Seoul 151-742 & Advanced Institutes of Convergence Technology Suwon 443-270 Republic of Korea Department of Ophthalmology College of Medicine Seoul National University Seoul 110-744 Republic of Korea

Nanoparticles can be involved in biological activities such as apoptosis, angiogenesis, and oxidative stress by themselves. In particular, inorganic nanoparticles such as gold and silica nanoparticles are known to inhibit vascular endothelial growth factor （VEGF）-mediated pathological angiogenesis. In this study, we show that anti-angiogenic effect of inorganic nanospheres is determined by their sizes. We demonstrate that 20 nm size gold and silica nanospheres suppress VEGF-induced activation of VEGF receptor-2, in vitro angiogenesis, and in vivo pathological angiogenesis more efficiently than their 100 nm size counterparts. Our results suggest that modulation of the size of gold and silica nanospheres determines their inhibitory activity to VEGF-mediated angiogenesis.

关键词： nanospheres anti-angiogenesis effects inorganic nanoparticles vascular endothelialgrowth factor pathological angiogenesis

Bee venom phospholipase A2 ameliorates amyloidogenesis and neuroinflammation through inhibition of signal transducer and activator of transcription-3 pathway in Tg2576 mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Translational Neurodegeneration 2019年第1期8卷 317-332页

作者： Hyeon Joo Ham sang-Bae Han Jaesuk Yun In Jun Yeo Young Wan Ham Se hyun Kim Pil-Hoon Park Dong-Young Choi jin Tae Hong College of Pharmacy and Medical Research Center Chungbuk National UniversityOsongsaengmyeong 1-roOsong-eupHeungdeok-guCheongjuChungbuk 28160Republic of Korea Department of Chemistry Utah Valley University800 W University PkwyOremUT 84058USA INISTst Co. LTD767Sinsu-roSuji-guYongin-si 16827Gyeonggi-doRepublic of Korea College of Pharmacy Yeungnam University280 Daehak RoadGyeonsanGyeongbuk 38541Republic of Korea

Background:Neuroinflammation and accumulation ofβ-amyloid(Aβ)play a significant role in the onset and progression of Alzheimer’s disease(AD).Our previous study demonstrated that signal transducer and activator of transcription-3(STAT3)plays a major role in neuroinflammation and ***:In the present study,we investigated the inhibitory effect of bee venom phospholipase A2(bvPLA2)on memory deficiency in Tg2576 mice,which demonstrate genetic characteristics of AD and the mechanism of its action at the cellular and animal *** in vivo study,we examined the effect of bvPLA2 on improving memory by conducting several behavioral tests with the administration of bvPLA2(1 mg/kg)to Tg2576 *** in vitro study,we examined the effect of bvPLA2 on amyloidogenesis and neuroinflammation by treating bvPLA2 on LPSactivated BV2 ***:We found that bvPLA2 alleviated memory impairment in Tg2576 mice,as demonstrated in the behavioral tests assessing *** the bvPLA2-treated group,Aβ,amyloid precursor protein(APP),and β-secretase 1(BACE1)levels and β-secretase activity were significantly *** of pro-inflammatory cytokines and inflammation-related proteins decreased in the brain of bvPLA2-treated group,whereas anti-inflammatory cytokines *** addition,bvPLA2 reduced STAT3 phosphorylation in the brains of the bvPLA2-treated *** the cellular level,bvPLA2 inhibits production of nitric oxide,pro-inflammatory cytokines,and inflammation-related proteins including ***,bvPLA2 inhibits the production of Aβin cultured BV-2 *** from the docking experiment,pull-down assay,and the luciferase assay show that bvPLA2 directly binds STAT3 and,thus,regulates gene expression ***,when the STAT3 inhibitor and bvPLA2 were administered together,the anti-amyloidogenic and anti-inflammatory effects were further enhanced than when they were administered ***:These results suggest that bvPLA2 could r

关键词： Bee venom phospholipase A2 Alzheimer’s disease Amyloidogenesis Neuroinflammation STAT3