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mechanically induced Ca^(2+) oscillations in osteocytes release extracellular vesicles and enhance bone formation

Bone Research 2018年第1期6卷 72-82页

作者： Andrea E.morrell Genevieve N.Brown Samuel T.Robinson Rachel l.Sattler Andrew D.Baik Gehua Zhen Xu Cao lynda F.Bonewald Weiyang Jin lance C.kam X.Edward Guo Bone Bioengineering laboratory Department of Biomedical Engineering Columbia University Center for musculoskeletal Research Department of Orthopaedic Surgery Johns Hopkins University Indiana Center for musculoskeletal Health Indiana University School of Medicine microscale Biocomplexity laboratory Department of Biomedical Engineering Columbia University

The vast osteocytic network is believed to orchestrate bone metabolic activity in response to mechanical stimuli through production of sclerostin, RANKl, and osteoprotegerin(OPG). However, the mechanisms of osteocyte mechanotransduction remain poorly understood. We've previously shown that osteocyte mechanosensitivity is encoded through unique intracellular calcium (Ca^(2+) ) dynamics. Here, by simultaneously monitoring Ca^(2+) and actin dynamics in single cells exposed to fluid shear flow, we detected actin network contractions immediately upon onset of flow-induced Ca^(2+) transients, which were facilitated by smooth muscle myosin and further confirmed in native osteocytes ex vivo. Actomyosin contractions have been linked to the secretion of extracellular vesicles(EVs), and our studies demonstrate that mechanical stimulation upregulates EV production in osteocytes through immunostaining for the secretory vesicle marker lysosomal-associated membrane protein 1(lAmP1) and quantifying EV release in conditioned medium, both of which are blunted when Ca^(2+) signaling was inhibited by neomycin. Axial tibia compression was used to induce anabolic bone formation responses in mice, revealing upregulated lAmP1 and expected downregulation of sclerostin in vivo. This load-related increase in lAmP1 expression was inhibited in neomycin-injected mice compared to ***-computed tomography revealed significant load-related increases in both trabecular bone volume fraction and cortical thickness after two weeks of loading, which were blunted by neomycin treatment. In summary, we found mechanical stimulation of osteocytes activates Ca^(2+) -dependent contractions and enhances the production and release of EVs containing bone regulatory proteins. Further, blocking Ca^(2+) signaling significantly attenuates adaptation to mechanical loading in vivo, suggesting a critical role for Ca^(2+) -mediated signaling in bone adaptation.

关键词： OPG conditioned medium Ca2+-dependent

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Ocular manifestations among HIV Infected Children in Ouagadougou, Burkina Faso

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Open Journal of Pediatrics 2016年第2期6卷 185-190页

作者： Caroline Yonaba Angèle Kalmogho Kongnimissom Apoline Sondo madi Nacoulma Kigocha Okengo Flore Ouédraogo Chantal Zoungrana Aissata Kabore Fla Koueta ludovic kam Département de Pédiatrie Centre Hospitalier Universitaire Yalgado Ouédraogo Ouagadougou Burkina Faso Service des maladies Infectieuses Centre Hospitalier Universitaire Yalgado Ouédraogo Ouagadougou Burkina Faso Neurology Service Muhimbili National Hospital Dar es Salaam Tanzania Service de Pédiatrie médicale Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle Ouagadougou Burkina Faso

Ocular manifestations among HIV infected children are diverse and global incidence varies from 7% to 75%. At this age, eye lesions are often unnoticed because of the incapacity to express eye discomfort. The purpose of this study is to describe ocular manifestations among HIV-infected children and hence associated factors in the Department of Paediatrics at the Yalgado Ouédraogo Teaching Hospital. This was a cross-sectional descriptive and analytical study conducted between July 2014 and December 2014. A complete ophthalmic examination was systematically done to all HIV-positive children attending the clinic, as part of their routine medical visit. The most recent socio-demographic, clinical, biological and treatment data were registered. Seventy-nine children had an ocular examination and among them 92.4% were on ARV treatment. The incidence of ocular manifestations was 46.7%. median age was 8 years old (interquartile 6 - 12 years old). Sex ratio was 1.3. The risk of ocular manifestations involvement among boys was twice than that of girls. more than half (59.5%) of children who had ocular problems had not expressed ocular discomfort. Ocular adnexal lesions were more common (35.4%) compared to eye segments (8.9%) lesions. Anterior segment and posterior segment lesions were statistically associated with immune system depression (p = 0.003 and 0.001). However, this relationship was not statistically significant (p = 0.15). Five out of seven children who had eye fundus lesions had CD4 count 3. Ocular manifestations were very common among HIV infected children in our context. Ophthalmic examination should be systematic at admission and regularly repeated during follow-up.

关键词： Paediatric HIV Ocular manifestations Ouagadougou

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Genomic,transcriptomic,and metabolomic analysis of Oldenlandia corymbosa reveals the biosynthesis and mode of action of anti-cancer metabolites

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Journal of Integrative Plant Biology 2023年第6期65卷 1442-1466页

作者： Irene Julca Daniela mutwil-Anderwald Vaishnervi manoj Zahra Khan Soak Kuan lai lay K.Yang Ing T.Beh Jerzy Dziekan Yoon P.lim Shen K.lim Yee W.low Yuen I.lam Seth Tjia Yuguang mu Qiao W.Tan Przemyslaw Nuc le m.Choo Gillian Khew loo Shining Antony kam James P.Tam Zbynek Bozdech maximilian Schmidt Bjoern Usadel Yoganathan Kanagasundaram Saleh Alseekh Alisdair Fernie Hoi Y.li marek mutwil School of Biological Sciences Nanyang Technological UniversitySingapore 639798Singapore Shared Analytics Singapore Institute of Food and Biotechnology Innovation(SIFBI)Agency for ScienceTechnology and Research(A*STAR)Singapore 138671Singapore Department of Biochemistry National University of SingaporeSingapore 117596Singapore Singapore Botanic Gardens Singapore 259569Singapore Department of Gene Expression Faculty of BiologyInstitute of Molecular Biology and BiotechnologyAdam Mickiewicz UniversityPoznan 61-614Poland IBG-4 Bioinformatics Forschungszentrum JülichJülich 52428Germany max-Planck-Institut für molekulare Pflanzenphysiologie Potsdam-Golm 14476Germany Center of Plant Systems Biology and Biotechnology Plovdiv 4000Bulgaria

Plants accumulate a vast array of secondary metabolites,which constitute a natural resource for *** corymbosa belongs to the Rubiaceae family,and has been used in traditional medicine to treat different diseases,including ***,the active metabolites of the plant,their biosynthetic pathway and mode of action in cancer are *** fill these gaps,we exposed this plant to eight different stress conditions and combined different omics data capturing gene expression,metabolic profiles,and anti-cancer *** results show that *** extracts are active against breast cancer cell lines and that ursolic acid is responsible for this ***,we assembled a high-quality genome and uncovered two genes involved in the biosynthesis of ursolic ***,we also revealed that ursolic acid causes mitotic catastrophe in cancer cells and identified three high-confidence protein binding targets by Cellular Thermal Shift Assay(CETSA)and reverse ***,these results constitute a valuable resource to further characterize the biosynthesis of active metabolites in the Oldenlandia group,while the mode of action of ursolic acid will allow us to further develop this valuable compound.

关键词： genome medicinal metabolomics mode of action transcriptome

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Antralization at the edge of proximal gastric ulcers:Does Helicobacter pylori infection play a role?

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World Journal of Gastroenterology 2003年第6期9卷 1265-1269页

作者： Harry Hua-Xinag Xia Shiu Kum lam Wai man Wong Wayne Hsing Cheng Hu kam Chuen lai Sau Hing Wong Suet Yi leung Siu Tsan Yuen Nicholas A.Wright Benjamin Chun-Yu Wong Department of medicine The University of Hong KongHong Kong SARChina Department of Pathology The University of Hong KongHong Kong SARChina Histopathology Unit London Research InstituteCancer ResearchUK

AIm: To determine the prevalence of antralization at the edge of proximal gastric ulcers, and the effect ofH. pylori eradication on the mucosal appearances.mETHODS: Biopsies were taken from the antrum, body and the ulcer edge of patients with benign proximal gastric ulcers before and one year after treatment. Gastric mucosa was classified as antral, transitional or body type.H. pylori positive patients receivedeither triple therapy, or omeprazole.RESUlTS: Patients with index ulcers in the incisura, body or fundus (n=116) were analyzed. Antral-type mucosa was more prevalent at the ulcer edge inH. pylori-positive patients ***-negative patients (93% vs 60%, OR=8.95,95%CI: 2.47-32.4, P=0.001). At one year, there was a significant reduction in the prevalence of antralization (from 93 % to 61%, P=0.004) at the ulcer edge in patients with H. pyloribeing eradicated. However, there was no difference in the prevalence of antralization at the ulcer edge in those with persistent infection.CONClUSION: H. pylori infection is associated with antralization at the edge of proximal gastric ulcers, which may be reversible in some patients after eradication of the infection.

关键词：胃溃疡幽门螺杆菌感染胃粘膜活组织检查

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Comparative Study of the Thermal and mechanical Properties of Foamed Concrete with local materials

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World Journal of Engineering and Technology 2022年第3期10卷 550-564页

作者： Adelaïde lareba Ouédraogo Sayouba Kabré Etienne malbila Abdoulaye Compaoré Ramatou Saré Paul Ilboudo Sié kam Bruno Korgo Dieudonné Joseph Bathiebo Florent P. Kieno Philippe Blanchard Département de Physique Laboratoire d’Energies Thermiques Renouvelables (LETRE) Faculté des Sciences Exactes et Appliquées (UFR/SEA) Ecole Doctorale Sciences et Technologies Université Joseph KI-ZERBO Ouagadougou Burkina Faso Département de Physique Laboratoire des Matériaux et Environnement (LAME) Faculté des Sciences Exactes et Appliquées (UFR/SEA) Ecole Doctorale Sciences et Technologies Université de Ouagadougou Joseph KI-ZERBO Ouagadougou Burkina Faso Département de Physique Université de Fada N’Gourma Fada Burkina Faso Département de Physique Chimie Institut de Recherche sur les Céramiques (IRCER) Limoge France

living in a habitat with comfort is requested by all. Cinder block bricks have poor thermal properties, leading people to use fan heaters and air conditioners to regain comfort. To overcome this problem of thermal discomfort in buildings, we used lightweight concrete such as foamed concrete which is a material that has improved thermal properties for thermal comfort. In addition, this material was compared with local materials used for the construction of buildings such as BTC, adobe and BlT mixed with binders. The results showed that foamed concrete is a material that has good thermal and mechanical properties compared to local materials mixed with binders. The foamed concrete having acceptable thermo-mechanical properties was that having a density of 930 kg/m3. It has a thermal resistance of 0.4 m2·K/W for a thickness of 20 cm. The foamed concrete having acceptable thermo-mechanical properties was that having a density of 930 kg/m3. It has a thermal resistance of 0.4 m2·K/W for a thickness of 20 cm. For sunshine on a daily cycle equal to 12 hours, the characteristic thickness achieved by this material is 7.29 cm. It also has a shallow depth of heat diffusion having a lower thickness than other materials. This shows that foamed concrete is a promising material for the construction of buildings.

关键词： Foamed Concrete Thermo-mechanical Properties Comparison local materi-als

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Effects of Nd and rotary forging on mechanical properties of AZ71 mg alloys

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Transactions of Nonferrous metals Society of China 2015年第10期25卷 3223-3231页

作者：陈贞光陈宇辰李宪宗陈锦修张浚荣国立台北科技大学材料科学与工程研究所台北市10608 鸿准精密工业股份有限公司新北市23680

The effects of Nd addition on the mechanical properties and plastic deformability of AZ71 mg alloys were investigated.0.5%-2.0%（mass fraction） Nd was added to AZ71 mg *** grain size and the amount of brittle β-mg17Al12 phase reduce with increasing the Nd addition,while nanosized AlxNdy precipitates *** combination with 32% rotary forging and subsequent annealing,the grain size of Nd-added AZ71 mg alloys reduces greatly from over 350 μm to below 30 μ*** tensile strength and ductility increase with the Nd addition up to 1.0%.The addition of Nd beyond 1.0% leads to the aggregations of rod-shaped Al11Nd3 and blocky Al2 Nd precipitates,thereby deteriorating both strength and *** 1.0% Nd-added AZ71 mg alloy shows tensile strength up to 253 mPa and elongation of 10.7%.It is concluded that adding 1.0% Nd to AZ71 mg alloy yields the optimum toughness,whether under as-cast or rotary forging and annealing conditions.

关键词： AZ71mg alloys Nd rotary forging mechanical properties recrystallization

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A Review on Bioactive Compounds Isolated from Euphorbia hirta l.

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American Journal of Plant Sciences 2023年第6期14卷 710-726页

作者： Roland Nâg-Tiéro meda Sami Eric kam Windmi Kagambega Eliasse Zongo Clarisse Ouedraogo Abdoulaye Segda Benjamin Kouliga Koama Franck Téounviel Somda Emmanuel Zongo Georges Anicet Ouedraogo laboratoire de Recherche et d’Enseignement en Santé et Biotechnologies Animales Université Nazi BONI Bobo-Dioulasso Burkina Faso laboratoire de Recherche en Biochimie INSP/Centre MURAZ Bobo-Dioulasso Burkina Faso laboratoire de médicine et Pharmacopée Traditionnelle Institut de Recherche en Sciences de la Santé Direction Régionale de Bobo-Dioulasso Bobo-Dioulasso Burkina Faso laboratoire de Biochimie Centre Hospitalier Universitaire Sourou SANOU Bobo-Dioulasso Burkina Faso

Euphorbia hirta l. is an annual medicinal herb throughout many tropical continents used to cure various diseases. Several studies have isolated many bioactive compounds from E. hirta. This study aimed at providing a collection of bioactive constituents in E. hirta. This review summarizes the extraction solvent, the structures and the properties of 38 bioactive phytochemicals isolated from E. hirta. It could help to understand the relationship existing between phytochemicals and their activities.

关键词： Euphorbia hirta Phytoconstituents Biological Activities Structures

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Ethnobotanical Survey of Appetite Suppressant Plants Used in Hauts-Bassins Areas of Burkina Faso

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Food and Nutrition Sciences 2022年第12期13卷 1001-1014页

作者： Emmanuel Zongo Roland Nâg-Tiero meda Yaya Gnanou Yaya Gnanou Sami Eric kam Benjamin Kouliga Koama Pataréyaoba Alassane Ouedraogo Eliasse Zongo Ollo Da Dramane Paré Georges Anicet Ouedraogo laboratoire de Recherche et d’Enseignement en Santé et Biotechnologies Animales Université Nazi BONI Bobo-Dioulasso Burkina Faso laboratoire de Biochimie Centre Hospitalier Universitaire Sourou SANOU Bobo-Dioulasso Burkina Faso laboratoire de médicine et Pharmacopée Traditionnelle Institut de Recherche en Sciences de la Santé Direction Régionale de l’Ouest Bobo-Dioulasso Burkina Faso laboratory of Biochemistry and Applied Chemistry (lABIOCA) UFR/SVT University of Ouagadougou Ouagadougou Burkina Faso

This study aimed to list the medicinal plants used as an appetite suppressant in Hauts-Bassins areas of Burkina Faso. An ethnobotanical survey was undertaken from September to November 2021 using a semi-structured questionnaire. To determine well-known families and species, some indices such as Family Importance Value (FIV) and Relative Frequency of Citation (RFC) were calculated respectively. Sixty-seven traditional healers (41 men and 26 women) have been interviewed. The age group from 41 to 60 years old was more represented (47.76%). Fifty-eight (58) plant species belonging to 29 families and 53 genera were recorded to have appetite suppressant properties. Fabaceae family (25%) was the most mentioned followed by Combretaceae (12%). The most mentioned species were Guierasenegalensis (7.64%), Parkiabiglobosa (6.18%), Annona senegalensis and Gardenia erubescens (5.35% for both). leaves and fruits had the highest frequencies of use with 41% and 25% respectively. The decoction (49.62%) was the main preparation method. The oral route was the only mode of drug administration. These results would contribute to strengthening the database on the medicinal plants used as an appetite suppressant by the traditional healers in Burkina Faso.

关键词： Ethnobotanical Survey Appetite Suppressant Plants Hauts-Bassins Areas Burkina Faso

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南亚季风长期动力:西藏新的高分辨率的古气候记录

南亚季风长期动力:西藏新的高分辨率的古气候记录

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中国古生物学会第20届学术年会

作者：唐领余沈才明 kam-biu liu J.T.Overpeck 中国科学院南京地质古生物研究所 louisiana State University Baton RougeLA 70803USA NOAA Paleoclimatology Program National Geophysical Date CenterBoulderCO.80303USA

1993-1995年中美科学家沿成都-康定-巴塘-八宿,波密-拉萨-那曲-嘉黎以及成都-兰州-格尔木-大柴旦3条路线进行野外考察和湖泊钻孔取样,共采集现代表土花粉样品202块(点),在6个小型湖泊中打钻取样,获得了18ka以来连续湖相沉积,累计岩芯25...

1993-1995年中美科学家沿成都-康定-巴塘-八宿,波密-拉萨-那曲-嘉黎以及成都-兰州-格尔木-大柴旦3条路线进行野外考察和湖泊钻孔取样,共采集现代表土花粉样品202块(点),在6个小型湖泊中打钻取样,获得了18ka以来连续湖相沉积,累计岩芯25m。这是恢复青藏高原末次最大盛冰期以来的古植被、古气候的理想材料。本研究花粉分析采样间距为2.5-4cm,磁化率、有机质含量和CaCO等取样间距为1cm,AmSC测年取样间距约为50cm。1现代花粉谱及转换函数

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基于教学模式视角的语言学习类大规模开放在线课程研究

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开放学习研究 2019年第5期24卷 9-15页

作者：黄德铭李锦昌张馨邈(译) 香港公开大学香港999077 北京大学医学继续教育学院在线教育中心

本论文从教学模式角度探讨大规模开放在线课程(mOOCs)为语言学习提供的机遇及其面临的挑战。mOOCs自2008年推出并逐渐兴起以来,被认为具有良好的教育发展前景,它们可以同时向大量学习者提供教学。目前mOOCs采用的主要教学模式是向学习... 详细信息

本论文从教学模式角度探讨大规模开放在线课程(mOOCs)为语言学习提供的机遇及其面临的挑战。mOOCs自2008年推出并逐渐兴起以来,被认为具有良好的教育发展前景,它们可以同时向大量学习者提供教学。目前mOOCs采用的主要教学模式是向学习者提供短视频及阅读材料进行自主学习、在线讨论(主要是学习者之间的课程内容讨论)以及自动评分的测验或考试,采用这种教学模式的mOOCs称为xmOOCs。然而,语言学习一般认为最重要的是提升语言技能,xmOOC教学模式对于语言学习的成效因而受到质疑。本研究分析语言学习类mOOCs的概况,包括从主流mOOC平台选取的123门语言学习类mOOCs,并根据课程采用的教学材料和学习活动类型对其教学模式进行分析。研究结果显示,这些mOOCs主要是教授英语的课程,其中80%的课程学习时长在6小时之内。大部分课程采用典型的xmOOC教学模式,其学习活动主要包括观看视频、阅读文本材料和进行自动评分测试。少于一半(43%)的课程设有在线讨论的学习环节,但其中仅有28%的课程有教学指导者参与讨论。尽管在技术上优化了课程模式,但目前语言学习类mOOCs与传统的语言学习远程课程在教学模式上没有明显的差异。然而,不可否认的是,计算机技术支持的语言学习以及mOOCs的庞大学习者群体形成的虚拟学习社区,为学习者提升语言技能提供了机遇。

关键词： mOOCs 语言学习教学模式

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