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Projection of China’s future runoff based on the CMIP6 mid-high warming scenarios

Science China Earth Sciences 2023年第3期66卷 528-546页

作者： Jiayue ZHOU Hui lu Kun YANG Ruijie JIANG Yuan YANG Wei WANG Xuejun ZHANG Department of Earth System Science Ministry of Education Key Laboratory of Earth System ModelingInstitute for Global Change StudiesTsinghua UniversityBeijing 100084China National Tibetan Plateau Data Center State Key Laboratory of Tibetan Plateau Earth System and Resource EnvironmentInstitute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China Center for Western Weather and Water Extremes Scripps Institution of OceanographyUniversity of California San DiegoSan Diego 92037USA Changjiang Institute of Survey PlanningDesign and ResearchWuhan 430010China State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin China Institute of Water Resources and Hydropower ResearchBeijing 100038China Research Center on Flood and Drought Disaster Reduction of the Ministry of Water Resources Beijing 100038China

The latest Coupled Model Intercomparison Project Phase 6(CMIP6)proposes new shared pathways(SSPs)that incorporate socioeconomic development with more comprehensive and scientific experimental designs;however,few studies have been performed on the projection of future multibasin hydrological changes in China based on CMIP6 *** this paper,we use the Equidistant Cumulative Distribution Function method(EDCDFm)to perform downscaling and bias correction in daily precipitation,daily maximum temperature,and daily minimum temperature for six CMIP6 models based on the historical gridded data from the high-resolution China Meteorological Forcing Dataset(CMFD).We use the bias-corrected precipitation,temperature,and daily mean wind speed to drive the variable infiltration capacity(VIC)hydrological model,and study the changes in multiyear average annual precipitation,annual evapotranspiration and total annual runoff depth relative to the historical baseline period(1985–2014)for the Chinese mainland,basins and grid scales in the 21st century future under the SSP2-4.5 and SSP5-8.5 *** study shows that the VIC model accurately simulates runoff in major Chinese basins;the model data accuracy improves substantially after downscaling bias correction;and the future multimodel-mean multiyear average annual precipitation,annual evapotranspiration,and total annual runoff depth for the Chinese mainland and each basin increase relative to the historical period in near future(2020–2049)and far future(2070–2099)under the SSP2-4.5 and *** new CMIP6-based results of this paper can provide a strong reference for extreme event prevention,water resource utilization and management in China in the 21st century.

关键词： CMIP6 VIC model EDCDFm Chinese basins Future hydrological changes

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Efficient strategies for changing the diapause character of silkworm eggs and for the germline transformation of diapause silkworm strains

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Insect Science 2012年第2期19卷 172-182页

作者： Ai-Chun Zhao Ding-Pei Long san-Yuan Ma Long-Xia Xu Mei-Rong Zhang Fang-Yin Dai Qing-You Xia Cheng lu Zhong-Huai Xiang The Key Sericultural Laboratory of Agricultural Ministry Institute of Sericulture and Systems BiologySouthwest UniversityChongqingChina

To overcome the disadvantages of current silkworm Bombyx mori transgenic technology, such as costly and time-consuming to maintain non-diapause transgenic silkworms, we report here on the development of treatments for the germline transformation of diapause silkworm strains. Our results showed that HC1 treatment within 3 h of oviposition was able to prevent the diapause of eggs from Japanese lineage diapause silkworm strains and was also suitable for germline transformation of the same strains. By incu- bating developing mother eggs from Chinese lineage diapause silkworm strains at 15℃ （15℃-IME）, we were able to prevent the diapause of their daughter eggs; a similar strategy （15℃-IMES） for the germline transformation of the same strains was that the mother eggs were incubated at 15℃, and the daughter eggs were then microinjected according to the conventional microinjection methods used for non-diapause eggs. By combining tempera- ture and light controls, the improved 15℃-IMES strategy prevented diapause in daughter eggs, and also enabled the germline transformation of both Japanese and Chinese lineage diapause silkworm strains. Although each of the strategies developed here has advantages and disadvantages, we suggest that the 15℃-IMES strategy is a good reference for the establishment of germline transformation technologies of other egg diapause insects. These new strategies for the efficient germline transformation of diapause silkworm strains are likely to improve the practical use of silkworm transgenic lines in sericulture and also highlight silkworm functional genomics research and its modeling.

关键词： Bombyx mori diapause functional genomics gerrnline transformation insect piggyBac

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Improving the surveillance and response system to achieve and maintain malaria elimination:a retrospective analysis in Jiangsu Province,China

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Infectious Diseases of Poverty 2022年第1期11卷 56-67页

作者： Yuanyuan Cao Guangyu lu Chris Cotter Weiming Wang Mengmeng Yang Yaobao Liu Cheng Liang Huayun Zhou Yan lu Jun Yan Guoding Zhu Jun Cao Key Laboratory of National Health Commission(NHC)On Parasitic Disease Control and Prevention Jiangsu Provincial Key Laboratory On Parasite and Vector Control TechnologyJiangsu Institute of Parasitic DiseasesWuxi214064JiangsuChina Institute of Public Health Medical College of Yangzhou UniversityJiangsu ProvinceYangzhou city225000China Malaria Elimination Initiative Institute for Global Health SciencesUniversity of California San FranciscoSan FranciscoCAUSA Department of Women’s and Children’s Health International Maternal and Child HealthUppsala UniversityUppsalaSweden Center for Global Health School of Public HealthNanjing Medical UniversityNanjing211166China Health and Quarantine Department Nanjing CustomsNanjing210001China Chinese Center for Disease Control and Prevention Beijing102206China

Background:Following initiation of China’s National Malaria Elimination Action Plan(NMEAP)in 2010,the'1-3-7’approach was developed and rolled out in China to facilitate the malaria elimination programme and accelerate malaria *** study aims to summarize and condense these experiences through a retrospective analysis in Jiangsu Province,which could be adapted and applied in other malaria elimination settings ***:A retrospective analysis of imported malaria cases into China identified through an improved surveillance and response system in Jiangsu Province was carried out for the period of *** improve the malaria surveillance and response system,Centers for Diseases Control and Prevention from the prefectures and counties in Jiangsu province conducted population-level health education to improve healthcare seeking behavior,strengthened capacity of health facilities to improve performance of malaria diagnosis and treatment,and raised the capacity of public health providers to improve implementation of the'1-3-7’*** variables were carried out by Chi square tests with Fisher’s exact ***:From 2001 to 2020,a total of 9,879 malaria cases were reported in Jiangsu *** 2012,no indigenous malaria cases have been reported in Jiangsu ***,in recent years,there has been a substantial increase of imported falciparum malaria *** 2012 and 2020,an estimated 61.57 million individuals have benefited from population-level health education in Jiangsu *** healthcare-seeking services among the 2,423 imported malaria cases,687(28.4%)and 1,104(45.6%)cases visited hospitals on the first day and the second day from symptom onset,respectively.A total of 1,502(61.9%)cases were diagnosed on the first day at medical *** Province achieved 100%,99.4%and 98.3%completion rate in terms of case detection and notification(within one day),case investigation(within three days)and foci

关键词： China 1-3-7 approach Surveillance and response Malaria Elimination Imported case Jiangsu Retrospective study

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Fractionated evaluation of immunohistochemical hormone receptor expression enhances prognostic prediction in breast cancer patients treated with tamoxifen as adjuvant therapy

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第1期11卷 1-9页

作者： Ching-hung LIN Huang-chun LIEN Fu-chang HU Yen-shen lu Sung-hsin KUO Ling-chu WE san-lin YOU Ann-lii CHENG King-jen CHANG Department of Oncology National Taiwan University Hospital Taiwan Taipei 10016 Department of Pathology National Taiwan University Hospital Taiwan Taipei 10016 National Center of Excellence for General Clinical Trial and Research National Taiwan University Hospital Taiwan Taipei 1001 6 Graduate Institute of Epidemiology College of Public Health National Taiwan University Taiwan Taipei 10055 Department of Surgery National Taiwan University Hospital Taiwan Taipei 10016

Objective:To compare the prognostic prediction between dichotomized and fractionated evaluations of hormone receptor expressions. Methods: Patients with stages I-III breast cancers, who received adjuvant tamoxifen, were enrolled. The expression of estrogen receptor (ER) and progesterone receptor (PR) was evaluated by immu-nohistochemistry (IHC). A fractionated score (F score), the percentage of positive-staining nuclei (0=none, 1=1%-10%, 2=11%-30%, 3=31%-50%, 4=51%-70%, and 5=71%-100%), was assigned to each case. The dichotomized scoring method defines an F score >1 as positive. The prognostic values of both scores were compared by multiple Cox's proportional hazard models of disease-free survival (DFS) and overall survival (OS). Results: Four hundred and sixteen patients with a median follow-up of 78.0 months were included. F scores for ER and PR correlated directly with DFS and OS. Although both the dichotomized and fractionated ER and PR scores were significantly associated with DFS and OS in univariate analyses, only fractionated ER and PR scores remained as independent prognostic factors of DFS and OS in the final multiple Cox's proportional hazard models. Conclusion: Fractionated IHC hormone receptor expression evaluation enhances the prognostic prediction compared with a dichotomized assessment.

关键词：孕激素受体受体表达乳腺癌辅助治疗预后评价他莫昔芬预测

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Density Functional Theory for Electrocatalysis

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Energy & Environmental Materials 2022年第1期5卷 157-185页

作者： Xiaobin Liao Ruihu lu Lixue Xia Qian Liu Huan Wang Kristin Zhao Zhaoyang Wang Yan Zhao State Key Laboratory of Silicate Materials for Architectures International School of Materials Science and EngineeringWuhan University of TechnologyWuhan 430070China Zhejiang University Hangzhou 310058China State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Center of Smart Materials and DevicesSchool of Materials Science and EngineeringWuhan University of TechnologyWuhan 430070China Lynbrook High School San Jose CA 95129USA The Institute of Technological Sciences Wuhan UniversityWuhan 430072China

It is a considerably promising strategy to produce fuels and high-value chemicals through an electrochemical conversion process in the green and sustainable energy *** for electrocatalytic reactions,including hydrogen evolution reaction(HER),oxygen evolution reaction(OER),oxygen reduction reaction(ORR),nitrogen reduction reaction(NRR),carbon dioxide reduction reaction(CO_(2)RR),play a significant role in the advanced energy conversion technologies,such as water splitting devices,fuel cells,and rechargeable metal-air *** low-cost and highly efficient electrocatalysts is closely related to establishing the composition-structure-activity relationships and fundamental understanding of catalytic *** functional theory(DFT)is emerging as an important computational tool that can provide insights into the relationship between the electrochemical performances and physical/chemical properties of *** article presents a review on the progress of the DFT,and the computational simulations,within the framework of DFT,for the electrocatalytic processes,as well as the computational designs and virtual screenings of new *** useful descriptors and analysis tools for evaluating the electrocatalytic performances are highlighted,including formation energies,d-band model,scaling relation,egorbital occupation,and free energies of ***,the remaining questions and perspectives for the development of DFT for electrocatalysis are also proposed.

关键词： analysis tools density functional theory descriptors electrocatalysis

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Rapid induction of PC3/BTG2 gene by hepatopoietin or partial hepatectomy and its mRNA expression in hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2009年第3期8卷 288-293页

作者： Zhang, Zhi-Min Wang, Ge Chen, Chuan Yang, Zhi-Xiang Jin, Feng san, Jin-lu Xu, Wen Li, Qiong Li, Zeng-Peng Wang, Dong Third Mil Med Univ Ctr Canc Inst Surg Res Daping Hosp Chongqing 400042 Peoples R China

BACKGROUND: The anti-proliferative gene, PC3 (pheochromocytoma cell 3)/BTG2 (B-cell translocation gene 2), is one of the early growth response genes and belongs to the BTG/Tob protein family. This study aimed to assess the effects of recombinant human hepatopoietin (HPO) and partial hepatectomy on rapidly induced expression of immediate-early genes and to investigate the expression of PC3/BTG2 mRNA in hepatocellular carcinoma (HCC) at different stages of progression. METHODS: After a rat model of partial hepatectomy was established, we investigated gene expression within I hour after 2/3 partial hepatectomy by representational difference analysis and in a primary cultured hepatocyte system. The expression levels of PC3/BTG2 from liver tissues of the rat model were assessed by RT-PCR and Northern blotting. Meanwhile, the expression of BTG2 mRNA in a tissue microarray of HCC was determined by in situ hybridization. RESULTS: The PC3/BTG2 gene was rapidly induced after 2/3 partial hepatectomy and its expression peaked within 1-2 hours after operation. HPO rapidly induced the expression of the genes c-fos, LRF-1, and PC3 in primary cultured rat hepatocytes, which might be one of the molecular mechanisms by which HPO stimulates hepatocyte proliferation. Positive BTG2 mRNA expression was detected in 71.19% (42/59) of the HCC samples and in 75% (3/4) of the normal liver tissue samples obtained from the region around the HCC tissues. PC3/BTG2 mRNA was located mainly in the cytoplasm of HCC cells and its expression was related to the degree of differentiation. CONCluSIONS: Recombinant human HPO and partial hepatectomy rapidly induce the expression of the PC3/BTG2 gene. PC3/BTG2 mRNA is highly expressed in HCC cells and its expression is related to the degree of cell differentiation. The abnormal expression of PC3/BTG2 is closely related to the genesis and development of HCC, so PC3/BTG2 may play an important role in these processes. (Hepatobilimy Pancreat Dis Int 2009; 8: 288

关键词： hepatopoietin liver regeneration hepatectomy hepatocellular carcinoma PC3/BTG2

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电针“智三针”对5xFAD小鼠Notch信号通路及突触可塑性的影响

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中国组织工程研究 2024年第32期28卷 5148-5153页

作者：温华能林润王逸潇王冰水刘璐刘传耀蔡灿鑫崔韶阳许明珠南方医科大学深圳医院康复医学科广东省深圳市518101 南方医科大学第三临床医学院广东省深圳市518101 广州中医药大学深圳医院(福田)康复医学科广东省深圳市518034

背景:阿尔茨海默病是以认知功能障碍为主要临床表现的退行性神经系统疾病,针刺是治疗阿尔茨海默病的传统特色疗法,但作用机制尚不明晰。目的:观察电针“智三针”对5xFAD小鼠Notch信号通路、β-淀粉样蛋白及突触可塑性的影响。方法:将16... 详细信息

背景:阿尔茨海默病是以认知功能障碍为主要临床表现的退行性神经系统疾病,针刺是治疗阿尔茨海默病的传统特色疗法,但作用机制尚不明晰。目的:观察电针“智三针”对5xFAD小鼠Notch信号通路、β-淀粉样蛋白及突触可塑性的影响。方法:将16只SPF级、雄性、6月龄5xFAD小鼠随机分为电针“智三针”组和模型组,每组8只,另取8只同条件C57BL/6小鼠作为野生对照组。电针“智三针”组进行电针“智三针”干预,每周5次,连续4周;模型组、野生对照组不进行干预。干预结束后,采用Morris水迷宫初步评价学习记忆能力;硫磺素S染色检测β-淀粉样蛋白斑块沉积情况;Western blot和qPCR检测跨膜受体蛋白Notch 1、Notch 1胞内段(Notch 1 intracellular domain,NICD)、Split多毛增强子1(hairy and enhancer of split 1,Hes 1)、Split多毛增强子5(hairy and enhancer of split 5,Hes 5)、突触生长蛋白(synaptophysin,SYN)、突触后密度蛋白95(postsynaptic density protein-95,PSD-95)及β-淀粉样蛋白的表达水平。结果与结论:①与模型组相比,野生对照组、电针“智三针”组小鼠逃避潜伏期缩短,穿越平台次数及目标象限停留时间增加(P<0.05);②与野生对照组相比,模型组小鼠海马区β-淀粉样蛋白斑块沉积显著增加,而电针“智三针”抑制了β-淀粉样蛋白斑块沉积(P<0.05);③与野生对照组相比,模型组小鼠海马组织中SYN、PSD-95、Notch 1、NICD、Hes 1及Hes 5 mRNA表达降低,β-淀粉样蛋白mRNA表达增加(P<0.05);与模型组相比,电针“智三针”组SYN、PSD-95、Notch 1、NICD、Hes 1及Hes 5 mRNA表达升高,β-淀粉样蛋白mRNA表达降低(P<0.05);④与野生对照组相比,模型组小鼠海马组织中SYN、PSD-95、Notch 1、NICD、Hes 1及Hes 5蛋白表达降低,β-淀粉样蛋白表达增加(P<0.05),与模型组相比,电针“智三针”组SYN、PSD-95、Notch 1、NICD、Hes 1及Hes 5蛋白表达增加,β-淀粉样蛋白表达降低(P<0.05);⑤结果表明,电针“智三针”能够改善5xFAD小鼠的学习记忆功能,其机制可能与抑制海马区β-淀粉样蛋白沉积及激活Notch信号通路从而增强神经突触可塑性有关。

关键词：阿尔茨海默病智三针电针 Notch信号通路 5xFAD小鼠突触可塑性 β-淀粉样蛋白

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宋银枝基于木郁土壅辨治更年期高血压的思路探讨

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广州中医药大学学报 2024年第9期41卷 2462-2466页

作者：陶洁李亮许禄华宋银枝(指导) 广州中医药大学第七临床学院深圳市宝安区中医院广东深圳518100

肝木与脾土互资互用,肝主疏泄、脾主运化协同配合是人体维持生命和机能的枢纽。若肝脾功能失调,肝失疏泄,脾失健运,出现木郁土壅的病理状态,则可影响肠道菌群的稳定性及神经系统的功能,出现脑-肠轴机制异常,从而导致更年期高血压的发生... 详细信息

肝木与脾土互资互用,肝主疏泄、脾主运化协同配合是人体维持生命和机能的枢纽。若肝脾功能失调,肝失疏泄,脾失健运,出现木郁土壅的病理状态,则可影响肠道菌群的稳定性及神经系统的功能,出现脑-肠轴机制异常,从而导致更年期高血压的发生与发展。宋银枝教授基于多年临证体会,提出木郁土壅可致更年期高血压,并结合现代研究分析其病理机制,探讨采用疏肝运脾法防治更年期高血压的思路。疏肝运脾之具体措施包括“健运脾土,祛痰化浊”“疏肝解郁,补益气血”“未病先防,调摄心神”,方药可选六君子汤、半夏白术天麻汤、逍遥散等。通过疏肝运脾,可使肝气调达,情志舒畅;脾肠健运,肠道菌群保持稳态平衡,膏脂代谢正常,使血压波动在正常范围。治疗更年期高血压可从肝脾入手,疏肝运脾是调治更年期高血压的切入点和关键所在。

关键词：更年期高血压木郁土壅脑-肠轴疏肝运脾六君子汤半夏白术天麻汤逍遥散宋银枝

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Dermcidin Enhances the Migration, Invasion, and Metastasis of Hepatocellular Carcinoma Cells In Vitro and In Vivo

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Journal of Clinical and Translational Hepatology 2022年第3期10卷 429-438页

作者： Fanghua Qiu Huajing Long lu Zhang Jieyuan Liu Zetian Yang Xianzhang Huang Department of Hospital Acquired Infection Control Affiliated TCM Hospital of Guangzhou Medical UniversityGuangzhouChina Guangzhou University of Chinese Medicine GuangzhouChina University of California San DiegoWarren CollegeSan DiegoCAUSA Department of Clinical Laboratory Second Affiliated HospitalGuangzhou University of Chinese MedicineGuangzhouChina

Background and Aims:Hepatocellular carcinoma(HCC)is a common primary liver neoplasm with high ***(DCD),an antimicrobial peptide,has been reported to participate in *** study assessed the effects and underlying molecular events of DCD overexpression and knockdown on the regulation of HCC progression in vitro and in ***:The serum DCD level was detected using enzyme-linked immunosorbent *** overexpression,knockdown,and Ras-related C3 botulinum toxin substrate 1(Rac1)rescue were performed in SK-HEP-1 cells using *** staining,quantitative PCR,and Western blotting were used to detect the expression of different genes and *** in HCC cell migration and invasion were detected by Transwell migration and invasion assays.A nude mouse HCC cell orthotopic model was employed to verify the in vitro ***:The level of serum DCD was higher in patients with HCC and in SK-HEP-1 *** overexpression caused upregulation of DCD,fibronectin,Rac1,and cell division control protein 42 homologue(Cdc42)mRNA and proteins as well as actin-related protein 2/3(Arp2/3)protein(but reduced Arp2/3 mRNA levels)and activated Rac1 and ***,DCD overexpression induced HCC cell migration and invasion in vitro,whereas knockout of DCD expression had the opposite effects.A Rac1 rescue experiment in DCD-knockdown HCC cells increased HCC cell migration and invasion and increased the levels of active Rac1/total Rac1,Wiskott-Aldrich syndrome family protein(WASP),Arp2/3,and *** overexpression induced HCC cell metastasis to the abdomen and liver in ***:DCD promotes HCC cell migration,invasion,and metastasis through upregulation of noncatalytic region of tyrosine kinase adaptor protein 1(Nck1),Rac1,Cdc42,WASP,and Arp2/3,which induce actin cytoskeletal remodeling and fibronectin-mediated cell adhesion in HCC cells.

关键词： Hepatocellular carcinoma Dermcidin Metastasis Gene regulation Cell adhesion

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Association of apelin genetic variants with type 2 diabetes and related clinical features in Chinese Hans

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Chinese Medical Journal 2009年第11期122卷 1273-1276页

作者： ZHANG Rong HU Cheng WANG Cong-rong MA Xiao-jing BAO Yu-qian XU Jing lu Jing-yi QIN Wen XIANG Kun-san JIA Wei-ping Shanghai Diabetes Institute Department of Endocrinology and Metabolism Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai 200233 China

Background Apelin is an adipokine that contributes to the pathogenesis of type 2 diabetes. The plasma levels of apelin increased in obese patients and diabetic subjects. This study aimed to investigate the effects of apelin genetic variants on type 2 diabetes and related quantitative traits. Methods We selected three single nucleotide polymorphisms （SNPs） that could capture all common variants in APLN gene region and genotyped them in 1892 type 2 diabetic patients and 1808 normal glucose regulation controls. The clinical features related to glucose metabolism were measured in the controls. The comparison of allele and genotype distribution in the cases and controls were performed by using X2 tests. The association between SNPs and quantitative traits were analyzed using Wilcoxon＇s rank-sum test. Results None of the SNPs or haplotypes showed evidence of association to type 2 diabetes. However, rs2235306 was nominally associated with fasting plasma glucose levels in the male subjects with normal glucose regulation （（4.93±0.03） vs （5.01±0.03） mmol/L, P=0.04）. No significant difference was observed between all three SNPs and other variables. Conclusions APLN SNP rs2235306 was associated with fasting plasma glucose levels in males. It suggests that APLN genetic variants may contribute to clinical features related to glucose metabolism in Chinese population.

关键词： apelin diabetes mellitus, type 2 polymorphism, single nucleotide

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