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Facilitating effects of berberine on rat pancreatic islets through modulating hepatic nuclear factor 4 alpha expression and glucokinase activity

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World Journal of Gastroenterology 2008年第39期14卷 6004-6011页

作者： Zhi-Quan Wang Fu-Er lu san-Hua Leng Xin-Sheng Fang Guang Chen Zeng-Si Wang Li-Ping Dong Zhong-Qing Yan Institute of Integrated Traditional Chinese and Weestern Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM： To observe the effect of berberine on insulin secretion in rat pancreatic islets and to explore its possible molecular mechanism. METHODS： Pdmary rat islets were isolated from male Sprague-Dawley rats by collagenase digestion and treated with different concentrations （1, 3, 10 and 30 μmol/L） of berberine or 1 μmol/L Glibenclamide （GB） for 24 h. Glucose-stimulated insulin secretion （GSIS） assay was conducted and insulin was determined by radioimmunoassay. 3-（4,5-Dimethylthiazol-2-yl）- 2,5-diphenyltetrazolium bromide （MTT） assay was performed to evaluate cytotoxicity. The mRNA level of hepatic nuclear factor 4 alpha （HAIF4α） was determined by reverse transcription polymerase chain reaction （RT-PCR）. Indirect immunofluorescence staining and Western blot analysis were employed to detect protein expression of HNF4α in the islets. Glucokinase （GK） activity was measured by spectrophotometric method. RESULTS： Berberine enhanced GSIS rather than basal insulin secretion dose-dependently in rat islets and showed no significant cytotoxicity on islet cells at the concentration of 10 μmol/L. Both mRNA and protein expressions of HNF4α were up-regulated by berberine in a dose-dependent manner, and GK activity was also increased accordingly. However, GB demonstrated no regulatory effects on HNF4α expression or GK activity. CONCluSION： Berberine can enhance GSIS in rat islets, and probably exerts the insulinotropic effect via a pathway involving HNF4α and GK, which is distinct from sulphonylureas （SUs）.

关键词： Berberine Sulphonylureas Hepatocyte nuclear factor 4 alpha Glucokinase Pancreatic islet

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Effects of surround suppression on response adaptation of V1 neurons to visual stimuli

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Zoological Research 2014年第5期35卷 411-419页

作者： Peng LI Cai-Hong JIN san JIANG Miao-Miao LI Zi-lu WANG Hui ZHU Cui-Yun CHEN Tian-Miao HUA College of Life Sciences Anhui Normal University

The influence of intracortical inhibition on the response adaptation of visual cortical neurons remains in debate. To clarify this issue, in the present study the influence of surround suppression evoked through the local inhibitory interneurons on the adaptation effects of neurons in the primary visual cortex （V1） were observed. Moreover, the adaptations of V1 neurons to both the high-contrast visual stimuli presented in the classical receptive field （CRF） and to the costimulation presented in the CRF and the surrounding nonclassical receptive field （nCRF） were compared. The intensities of surround suppression were modulated with different sized grating stimuli. The results showed that the response adaptation of V1 neurons decreased significantly with the increase of surround suppression and this adaptation decrease was due to the reduction of the initial response of V1 neurons to visual stimuli. However, the plateau response during adaptation showed no significant changes. These findings indicate that the adaptation effects of V1 neurons may not be directly affected by surround suppression, but may be dynamically regulated by a negative feedback network and be finely adjusted by its initial spiking response to stimulus. This adaptive regulation is not only energy efficient for the central nervous system, but also beneficially acts to maintain the homeostasis of neuronal response to long-presenting visual signals.

关键词： Surround suppression V1 neurons Response adaptation Cat

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A novel CHS/ALG bi-layer composite membrane with sustained antimicrobial efficacy used as wound dressing

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Chinese Chemical Letters 2010年第8期21卷 1011-1014页

作者： Yang Dong Hong Zhuo Liu lu Xu Gang Li Zi Ning Ma Fei Han Hui Min Yao Yah Hui Sun san Ming Li School of Pharmaceutics Shenyang Pharmaceutical University Shenyang 110016 China China Criminal Police University Shenyang 110035 China

A membrane composed of an alginate（ALG） layer and a chitosan（CHS） layer with sustained antimicrobial efficacy was *** HC1（CIP） was incorporated into the ALG *** feature of the composite membrane was analyzed by scanning electron microscopy（SEM）.Water uptake capacity,in vitro drug release,and in vitro antimicrobial activity were *** composite membrane exhibited perfect binding characteristic between the two *** water uptake capacity of all the membranes was above 800%.The CIP could release from the composite membranes for 48 *** membrane could control the bacterial growth *** results suggested that this CHS/ALG composite membrane incorporated with CIP had the potential for wound dressing application.

关键词： Chitosan Alginate Composite membrane Ciprofloxacin HCl Sustained release Antimicrobial efficacy

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Two-Dimensional Cavity Resonant Modes of Si Based Bragg Reflection Ridge Waveguide

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Chinese Physics Letters 2011年第6期28卷 225-228页

作者： CHEN san.lu Hong-Yan CHEN Kun-Ji XU Jun MA Zhong-Yuan LI Wei HUANG Xin-Fan School of Physics and Electronic Information Huaibei Normal UniversityHuaibei 235000 State Laboratory of Solid State Microstructures and Department of Physics Nanjing UniversityNanjing 210093

Si-based ridge-waveguides with Bragg reflectors are fabricated based on our *** resonant peaks could be obviously identified from the photoluminescence spectra,and field patterns of these resonant peaks,simulated by the finite difference time domain(FDTD)method,confirm that these peaks originate from cavity *** resonant wavelengths and spatial angular distribution are given by the resonant models,which agree well with the experimental ***,a simple method is proposed to testify the experimental and theoretical *** devices based on Bragg reflectors may have potential applications in light-emitting diodes,lasers and integrated photonic circuits.

关键词： Waveguide resonant diodes

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Therapeutic effects of berberine in impaired glucose tolerance rats and its influence on insulin secretion

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Acta Pharmacologica Sinica 2004年第4期25卷 98-104页

作者： san-hua LENG, Fu-er lu2, Li-jun XU Institute of Integrated Traditional Chinese & Western Medicine, Tongji Hospital, TongjiMedical College, Huazhong University of Science & Technology, Wuhan 430030, China Institute of Integrated Traditional Chinese & Western Medicine Tongji Hospital Tongji Medical College Huazhong University of Science & Technology Wuhan 430030

AIM: To explore the anti-diabetic effects of berberine and its influence on insulin secretion. METHODS: Impaired glucose tolerance rats induced by iv injection of streptozotocin 30 mg/kgwere treated with berberine 187.5 and 562.5 mg/kg while fed with high fat laboratory chow. After rats were treated for 4 weeks, oral glucose tolerance was determined, and for 8 weeks, the fasting blood glucose, insulin, lipid series were determined. In insulin secretion experiments, berberine 93.75, 187.5, and 562.5 mg/kg was administered orally to BALB/c mice at a bolus. The murine serum was collected 2 h after the berberine administration for insulin determination. Insulin released from HIT-T15 cells and pancreatic islets incubated with berberine 1-100 μmol/L for 12 h was determined. RESULTS: The levels of fasting blood glucose (7.4±1.5 or 7.3±1.3 vs 9.3±1.3 mmol/L), triglycerides (0.61±0.22 or 0.63± 0.17 vs 1.8±0.7 mmol/L), total cholesterol (1.8±0.3 or 1.9±0.3 vs 2.2±0.2 mmol/L), free fatty acid (456±93 or 460±72 vs 550±113 μmol/L) and apolipoprotein B (0.37±0.02 or 0.42±0.05 vs 0.46±0.04 g/L) were reduced greatly in berberine-treated groups at doses of 187.5 and 562.5 mg·kg-1·d-1, respectively as compared with those in control group (Plucose tolerance was improved. In vitro experiment showed that berberine 1-10 μmol/L facilitated insulin secretion of HIT-T15 cells and murine pancreatic islets in a dose-dependent manner. Meanwhile murine serum insulin level (27.5±2.7 or 29±4 or 29±4 vs 24.3±2.8 pIU/L) was undoubtedly promoted and blood glucose (4.52±0.31 or 4.45±0.29 or 4.30±0.19 vs 4.87±0.21 mmol/L) was reduced after berberine administration at doses of 93.75, 187.5, and 562.5 mg/kg, respectively in the BALB/c mice. CONCluSION: Berberine possesses anti-diabetic effec

关键词： berberine diabetes mellitus glucose tolerance test insulin HIT-T15 cells pancreatic islets lipids

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MLH1 promoter germline-methylation in selected probands of Chinese hereditary non-polyposis colorectal cancer families

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World Journal of Gastroenterology 2008年第48期14卷 7329-7334页

作者： Heng-Hua Zhou Shi-Yan Yan Xiao-Yan Zhou Xiang Du Tai-Ming Zhang Xu Cai Yong-Ming lu san-Jun Cai Da-Ren Shi Department of Pathology Cancer Hospital/Institute Fudan University Department of Oncology Shanghai Medical College Fudan University Shanghai 209032 China Department of Gastroenterology Xinhua Hospital Shanghai Jiaotong University School of Medicine Shanghai 200092 China Department of Abdominal Surgery Cancer Hospital/Institute Fudan University Shanghai 200032 China

AIM： To detect the MLH1 gene promoter germline- methylation in probands of Chinese hereditary non- polyposis colorectal cancer （HNPCC）, and to evaluate the role of methylation in MLH1 gene promoter and molecular genetics in screening for ***： The promoter germline methylation of MLH1 gene was detected by methylation-specific PCR （MSP） in 18 probands from unrelated HNPCC families with high microsatellite-instability （MSI-H） phenotype but without germline mutations in MSH2, MLH1 and MSH6 genes. At the same time, 6 kindreds were col- lected with microsatellite-stability （MSS） phenotype but without germline mutations in MSH2, MIH1 and MSH6 genes as controls. The results of MSP were confirmed by clone sequencing. To ensure the reliability of the results, family H65 with nonsense germline mutation at c.2228C 〉 A in MSH2 gene was used as the negative control and the cell line sw48 was used as the known positive control along with water as the blank control. Immunochemical staining of MIH1 protein was performed with Envision two-step method in those patients with aberrant methylation to judge whether the status of MLH1 gene methylation affects the expression of MLH1 ***： Five probands with MIH1 gene promoter methylation were detected in 18 Chinese HNPCC families with MSI-H phenotype but without germline mutations in MSH2, MLH1 and MSH6 genes. Two of the five probands from families H10 and H29 displayed exhaustive-methylation, fulfilling the Japanese criteria （JC） and the Amsterdam criteria （AC）, respectively. The other 3 probands presented part-methylation fulfilling the AC. Of the 13 probands with unmethylation phenotype, 8 fulfilled the JC and the Bethesda guidelines （BG）, 5 fulfilled the AC. The rate of aberrant methylation in MLH1 gene in the AC group （22.2%, 4/18） was higher than that in the JC/BG groups （5.6%, 1/18） in all HNPCC families with MSI-H phenotype but without germline mutations in PISH2, PIIH1 and MSH6 genes. However, no proband with m

关键词： Hereditary non-polyposis colorectal cancer MLH1 Methylation Germline Methylation-specific PCR Microsatellite phenotype

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Aquaporin-4 deficiency attenuates acute lesions but aggravates delayed lesions and microgliosis after cryoinjury to mouse brain

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Neuroscience Bulletin 2012年第1期28卷 61-68页

作者： Wen-Zhen Shi Chun-Zhen Zhao Bing Zhao Xiao-Liang Zheng san-Hua Fang Yun-Bi lu Wei-Ping Zhang Zhong Chen Er-Qing Wei Department of Pharmacology and Institute of Neurobiology School of Medicine Zhejiang University Hangzhou 310058China

Objective To determine whether aquaporin-4 （AQP4） regulates acute lesions, delayed lesions, and the associated microglial activation after cryoinjury to the brain. Methods Brain cryoinjury was applied to AQP4 knockout （KO） and wild-type mice. At 24 h and on days 7 and 14 after cryoinjury, lesion volume, neuronal loss, and densities of microglia and astrocytes were determined, and their changes were compared between AQP4 KO and wild-type mice. Results Lesion volume and neuronal loss in AQP4 KO mice were milder at 24 h following cryoinjury, but worsened on days 7 and 14, compared to those in wild-type mice. Besides, microglial density increased more, and astrocyte proliferation and glial scar formation were attenuated on days 7 and 14 in AQP4 KO mice. Conclusion AQP4 deficiency ameliorates acute lesions, but worsens delayed lesions, perhaps due to the microgliosis in the late phase.

关键词： aquaporin-4 gene deficiency cryoinjury microglia astrocyte

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基于仿生的增韧环氧树脂的冲蚀磨损特性研究

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表面技术 2024年第13期53卷 118-127,138页

作者：徐林红沈东杨皓卢禄华邓州州中国地质大学(武汉)机械与电子信息学院武汉430074 厦门三烨清洁科技股份有限公司福建厦门361101

目的针对钾盐输送过程中钾盐颗粒对设备造成的冲蚀磨损问题,以及具有较好可溶性和吸湿性的钾盐易黏附于金属设备,从而造成的腐蚀和堵塞问题,将耐冲蚀磨损、耐腐蚀的高分子复合材料与耐冲蚀磨损仿生结构相结合对冲蚀关键零件进行设计,以... 详细信息

目的针对钾盐输送过程中钾盐颗粒对设备造成的冲蚀磨损问题,以及具有较好可溶性和吸湿性的钾盐易黏附于金属设备,从而造成的腐蚀和堵塞问题,将耐冲蚀磨损、耐腐蚀的高分子复合材料与耐冲蚀磨损仿生结构相结合对冲蚀关键零件进行设计,以提高散料输送过程中相关部件表面的耐冲蚀磨损性能和防黏附性能。方法利用自备的疏水二氧化硅对环氧树脂进行增韧处理,结合贝壳表面肋条结构及鲨鱼表皮盾鳞的特殊排列方式对冲蚀表面进行仿生设计,再利用翻模法在增韧环氧树脂表面制备耐冲蚀仿生结构,完成接触角的测量,以及表面耐磨性、表面硬度、耐冲蚀磨损性能等测试,探究疏水二氧化硅颗粒质量分数、仿生结构对环氧树脂综合性能的影响。结果增加疏水二氧化硅的含量,可以提高试样的疏水性;相较于无仿生结构试样,贝壳/鲨鱼复合仿生结构试样的耐磨性能更强,其质量损失率由0.22%降至0.072%。同时,在疏水二氧化硅的质量分数为4%时,冲蚀率最低,仅为1.4%。结论疏水二氧化硅的质量分数为4%的增韧环氧树脂贝壳/鲨鱼仿生结构试样的综合性能最优,其疏水性、耐磨性、耐冲蚀性能均得到提升。

关键词：冲蚀磨损仿生结构增韧环氧树脂疏水二氧化硅

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Object oriented land cover classification using ALS and GeoEye imagery over mining area

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中国有色金属学会会刊：英文版 2011年第S3期21卷 733-737页

作者： YU Hai-yang, CHENG Gang, GE Xiao-san, lu Xiao-ping Key Laboratory of Mine Spatial Information Technologies of State Bureau of Surveying and Mapping, Henan Polytechnic University, Jiaozuo 454000, China Key Laboratory of Mine Spatial Information Technologies of State Bureau of Surveying and Mapping Henan Polytechnic University Jiaozuo 454000 China

An object oriented coal mining land cover classification method based on semantically meaningful image segmentation and image combination of GeoEye imagery and airborne laser scanning (ALS) data was presented. First, DEM, DSM and nDSM (normalized Digital Surface Model, nDSM) were extracted from ALS data. The GeoEye imagery and DSM data were combined to create segmented objects based on neighbor regions merge method. Then 10 kinds of objects were extracted. Different kinds of vegetation objects, including crop, grass, shrub and tree, can be extracted by using NDVI and height value of nDSM. Water and coal pile field was extracted by using NDWI and the standard deviation of DSM method. Height differences also can be used to distinguish buildings from road and vacant land, and accurate building contour information can be extracted by using relationship of neighbor objects and morphological method. The test result shows that the total classification accuracy of the presented method is 90.78% and the kappa coefficient is 0.891 4.

关键词： airborne laser scanning GeoEye nDSM object oriented classification mining areas

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Energy Budgets of the Dipolarization Front in Magnetic Reconnection through Kinetic Simulations

Energy Budgets of the Dipolarization Front in Magnetic Recon...

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2023年中国地球科学联合学术年会

作者： Shu Yukang lu san.lu Quanming Ding Weixing University of Science and Technology of China

Magnetic reconnection is a fundamental energy converting process in space plasma and astronomy. During the process, magnetic energy is released and particles are accelerated. Former studies[1,2] have shown that energy conversion is not limited near the reconnection site;the dipolarization front (DF) takes a large proportion of energy conversion. It becomes intriguing how magnetic energy is converted to ions and electrons at the DF.

关键词： Energy Budgets of the Dipolarization Front in Magnetic Reconnection through Kinetic Simulations

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