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Multi-environment BSA-seq using large F3 populations is able to achieve reliable QTL mapping with high power and resolution: An experimental demonstration in rice

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The Crop Journal 2024年第2期12卷 549-557页

作者： Yan Zheng Ei Ei Khine Khin Mar Thi Ei Ei Nyein Likun Huang Lihui Lin Xiaofang Xie Min Htay Wai Lin Khin Than oo Myat Myat Moe san san Aye Weiren Wu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Fujian Provincial Key Laboratory of Crop Breeding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Department of Botany Mawlamyine UniversityMon StateMawlamyineMyanmar Department of Botany Dagon UniversityNorth DagonYangonMyanmar

Bulked-segregant analysis by deep sequencing(BSA-seq) is a widely used method for mapping QTL(quantitative trait loci) due to its simplicity, speed, cost-effectiveness, and efficiency. However, the ability of BSA-seq to detect QTL is often limited by inappropriate experimental designs, as evidenced by numerous practical studies. Most BSA-seq studies have utilized small to medium-sized populations, with F2populations being the most common choice. Nevertheless, theoretical studies have shown that using a large population with an appropriate pool size can significantly enhance the power and resolution of QTL detection in BSA-seq, with F_(3)populations offering notable advantages over F2populations. To provide an experimental demonstration, we tested the power of BSA-seq to identify QTL controlling days from sowing to heading(DTH) in a 7200-plant rice F_(3)population in two environments, with a pool size of approximately 500. Each experiment identified 34 QTL, an order of magnitude greater than reported in most BSA-seq experiments, of which 23 were detected in both experiments, with 17 of these located near41 previously reported QTL and eight cloned genes known to control DTH in rice. These results indicate that QTL mapping by BSA-seq in large F_(3)populations and multi-environment experiments can achieve high power, resolution, and reliability.

关键词： BSA-seq QTL mapping Large F3 population Multi-environment experiment Cross-validation

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Distribution Pattern and Seasonal Variation of Bird Species from Semi-Urban Area of Taungoo Environs

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American Journal of Operations Research 2020年第4期10卷 111-121页

作者： san san oo Nyo Nyo Aung Aye Aye Khine Myo sandar Win san Aung Tun Tun Myint Khin Khin Yone Nwe Nwe Aung Khin Maw Maw Myint Aung Aung Khin Mar Lwin Soe Soe Win Nyunt Nyunt oo Biak Khun Aye Zoology Department University of Yangon Yangon Myanmar Zoology Department East Yangon University Yangon Myanmar Zoology Department Taungoo Uniersity Taungoo Myanmar Zoology Department Kyaing Tong Uniersity Kyaing Tong Myanmar Zoology Department Dagon University Yangon Myanmar Department of Zoology Hpaan University Hpann Myanmar Zoology Department Hakar Uniersity Hakar Myanmar

The environs of Taungoo Township, Bago Region are one of the high biodiversity areas in Myanmar, locating eastern side of the high mountain ranges of Middle Yoma. The dense of every green and deciduous forest in the middle Middle Yoma were logging since 10 - 20 years ago. The birds inhabiting in that area have to refuge in suitable habitats nearby. The resent status of bird species is needed to assess the diversity of birds with the respect to distribution and seasonal variation after the construction of Yangon-Nepyitaw, Myanmar after twenty years ago. A total of 50 species of 27 genera, 22 families under nine orders were recorded. That represented 44 resident species (32 terrestrial species and 12 water bird species), and 6 species of migratory birds (1 terrestrial species and 5 water bird species). As habitat utilization, 24 bird species in paddy fields, 21 species in scattered trees, 33 species in wetland and 25 species in forest habitat types were investigated. Some species used more than one habitat types. According to the assessment of abundance categories based on the calculation of individual numbers of the birds, abundant category (3 species), common bird (1 species), frequent category (5 species), uncommon category (3 species) and rare species (28 species) were categorized. Hence, bird community was still sustainable in this area. In the aspect of conservation status, all recorded bird species were under least concern categories, according to the IUCN Redlist in the study area.

关键词： Species Diversity Birds Habitat Utilization Taungoo Environs

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Seasonal Prevalence of <i>Aedes aegypti</i>in Semi-Urban Area of Yangon Region, Myanmar

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Advances in Entomology 2020年第3期8卷 107-116页

作者： san san oo Tin Lay Mon Nyo Nyo Aung Thida Ei Toe Toe Soe Aye Aye Su Khin Khin Soe Khin Mar Lwin Thin Thin Soe Myat Lwin Htwe Department of Zoology Yangon University Yangon Myanmar Department of Zoology Kyaing Tong University Kyaing Tong Myanmar

Prevalence rate of Aedes aegypti was conducted in 20 houses from semi-urban areas of Yangon Region. Larval surveys were done at indoors and outdoors water containers of five types. Prevalence rate of larval density larvae was investigated monthly by standard indices. The highest infestation rate of the container index (CI) was in June 2018 (56.52%), the second highest was in July 2017 (48.36%) and the lowest rate was in April 2017 (5.07%);those of the Jar index (JI) was highest (36.49%) in June and second highest rate (23.8%) was in October 2017. Reasoning the Metal drum (MI) was highest (13.95%) in June 2018 and second highest (6.25%) was in July 2017. The larval infestation rate of Earthen pot (EI) was highest (42.1%) in July 2017. The larval incident rate in almost all indices showed that the highest rate was at the beginning of monsoon season, in June and July, while in the remaining months, the larval incident rate was found to decrease due to the application of insecticides in the study area by the Township Public Health Department. However, the application of insecticides did not cover all the breeding sites of the mosquitoes, the water puddles under their houses were left to apply the insecticides. The positive larval incident rate was assessed by Household (HI), Container index (CI), Breteau index (BI). The highest and second highest positive larval incident rates were all in June 2018 and July 2017 in all indices, HI (27.3% and 23.4%), CI (56.52% and 48.36%), BI (17.56% and 16.79%) and SI (28.49% and 24.38%) respectively. The lowest rate in all indices was 2.56% (HI), 5.07% (IC), 2.67% (BI) and 1.91% (SI) in April. In this study, the fluctuation of indices of infestation rates and positive larval index value was positively correlated in similar trends in the study months. The reason for difficult control measure depends on the water sources under their houses and remains stagnant throughout the year, even in the dry season. High incident and death r

关键词： Aedes aegypti Infestation Rate Positive Larval Indices South Dagon Township

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Diversity of Insect Pest and Predator Species in Monsoon and Summer Rice Fields of Taungoo Environs, Myanmar

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Advances in Entomology 2020年第3期8卷 117-129页

作者： san san oo Khin Myat Hmwe Nyo Nyo Aung Aye Aye Su Khin Khin Soe Tin Lay Mon Khin Mar Lwin May Myat Thu Thin Thin Soe Myat Lwin Htwe Zoology Department University of Yangon Yangon Myanmar Zoology Department Kyaing Tong University Kyaing Tong Myanmar Zoology Department Dagon University Yangon Myanmar

Paddy fields are natural and artificial wetland ecosystems that supply rice for the people and provide the wildlife especially insect diversity of different functional aspects. A total of 71 insect species belonging to 40 families under eight orders were observed during the study period. Among the 71 insect species, 18 species of beetles, nine species of bugs, eight species of dragonfly, five species of butterflies, four species of leafhoppers, plant hoppers and moths, three borer and spiders, two crickets, one species of skippers, grass hopper, hispa, ant, weevil, hairy caterpillar, leaf roller, katydid, thrips, maggot and water boatmen were recorded in the study sites. Total of 41 species of pests, 18 species of predators and 12 species of beneficial species (they function as pollinating the flowering plants in the paddy field wetland ecosystem) were recorded in the study sites. In the monsoon season, the 41 species of pest species, 18 species of predator species and 12 species of beneficial species were recorded from monsoon rice field. According to Shannon Evenness value (H'/S = -0.012564), the data showed that the insect species recorded from the one habitat was not the similar to another. In summer paddy fields, 36 species of pest species, 16 species of predator species and 9 species of beneficial species were recorded. Total arthropod insect species were recorded 61 species from the dry rice fields. According to Shannon Evenness value (H'/S = -0.000120), the data showed that the insect species recorded from the one habitat was not the similar to another. Population growth and duration of life cycle of insects is mainly dominated by the temperature, the duration of life cycle is shorter in the high temperature than in the low temperature.

关键词： Indent Insect Species Diversity Pest and Predators Seasonal Variation Paddy Filed Taungoo

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Fish Species Diversity in Upstream and Downstream of Sittaung River,Bago Region,Myanmar

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Journal of Environmental Science and Engineering(A) 2020年第4期9卷 140-148页

作者： Toe Toe Soe san san oo Khin Thuzar Win Nyo Nyo Aung Aye Aye Su Khine Wai Yin Nu Hninn Kyaing Win Win Than Department of Zoology University of YangonKamayut Township 11041YangonMyanmar Department of Zoology University of TaungooTaungoo08101Bago RegionMyanmar Myeik University Kalwin Ward14053MyeikTanintharyi RegionMyanmar

This research was conducted to generate a primary database on ichthyofauna,distribution and diversities in the upstream and downstream segments of Sittaung River in Bago Region of *** study contributes to the information needed on the distribution of river fish in *** diversities are assessed by calculating the various diversities indices between December,2018 and November,2019.A total of 65 fish species belonging to 9 orders,23 families and 49 genera were *** 65 fish species were found in upstream segment but only 60 species of them were found in downstream segment of the *** all study sites,Shannon diversity index had value above 3 indicating that the structure of habitat was stable and ***’s index values were close to 1 in all sites indicating that the species richness in the region was *** abundance all of above 5%of total catch of Mystus vittatus,***,*** and Erethistes pusillus in their feeding habitat suggested that their upstream and downstream habitats provided suitable feeding *** bengalensis,Tenualosa ilisha,Mystus gulio,Rita rita and Silonia silondia were least abundant all below 0.1%of total catch showing they could be impacted by unwise anthropogenic *** fish species diversity is thus very important to conserve and manage and promote the health of river ecosystem that is necessary for the migration of fish species.

关键词： River fish diversity conserve and manage promoting the health of river ecosystem

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New Amides and Phenylpropanoid Glucosides from the Fruits of Piper retrofractum

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Natural Products and Bioprospecting 2019年第3期9卷 231-241页

作者： Rong Tang Ya-Qiong Zhang Dong-Bao Hu Xue-Fei Yang Jun Yang Myint Myint san Thaung Naing oo Yi Kong Yue-Hu Wang Key Laboratory of Economic Plants and Biotechnology and the Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of BotanyChinese Academy of SciencesKunming 650201People’s Republic of China School of Life Science&Technology China Pharmaceutical UniversityNanjing 210009People’s Republic of China University of Chinese Academy of Sciences Beijing 100049People’s Republic of China School of Chemical Biology and Environment Yuxi Normal UniversityYuxi 653100People’s Republic of China Southeast Asia Biodiversity Research Institute Chinese Academy of SciencesYezinNay Pyi Taw 05282Myanmar Forest Research Institute YezinNay Pyi Taw 05282Myanmar

Two new amides(E)-N-cinnamoyl-2-methoxypiperidine(1)and(R)-1-(2-oxopyrrolidin-3-yl)-5,6-dihydropyridin-2(1H)-one(2),four new amide glucosides,retrofractosides A-D(3-6),and two new phenylpropanoid glucosides,retrofractosides E(7)and F(8),together with 24 known compounds(9-32)were isolated from the fruits of Piper *** chemical structures of these new compounds were elucidated based on extensive spectroscopic *** of these isolates(1-32)were evaluated for inhibitory activity against mouse platelet aggregation induced by the peptide AYPGKF-NH2.(E)-N-(Tetrahydro-2H-pyran-2-yl)cinnamamide(9)showed a weak inhibitory efect,with an inhibition ratio of 52.0%at a concentration of 150μM.

关键词： Piper retrofractum Piperaceae Antiplatelet Amides Phenylpropanoids

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Mechanisms of root-soil reinforcement in bioembankments of sloping farmland in the purple hilly area,China

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Journal of Mountain Science 2019年第10期16卷 2285-2298页

作者： JIN Hui-fang SHI Dong-mei ZENG Xiao-ying WANG san-shu DUAN Teng LOU Yi-bao College of Resources and Environment Institute of Soil and Water Conservation and Eco-environmentSouthwestUniversityChongqing400715China Chongqing Institute of Forest Science Chongqing400036China Chongqing Surveying and Design Institute of Water Resources Electric Power and ArchitectureChongqing400020China

Bio-embankment is an important soil and water conservation measure in the purple hilly area in China,which can effectively improve the ability of cultivated soil layers to resist rainfall erosion and runoff *** contrast,the ecological effect of bioembankment depends on the stability of the earth *** the natural grass bank as a control(CK),the root distribution,root tensile properties and shear resistance of root-soil composites for 3 typical soil and water conservation bio-embankments,namely,Morus alba Linn(Morns),Zanthoxylum bungeanum Maxim(Zanthoxylum)and Medicago sativa(Medicago)were *** results included the following:(1)The root system of the bio-embankments generally decreased in extent with the soil depth;fine roots in the o-io cm depth were most prevalent and significantly higher than those at the other depths,and coarse roots were mainly distributed in the o-30cm layer.(2)The stress-strain curves of the roots of each bio-embankment were single-peak curves without clear strain softening *** smaller the root diameters were,the smoother the stress-strain curves,and the lower the capability of the earth bank to resist *** larger the root diameters were,the lower the tensile *** average root tensile force was highest for Zanthoxylum(73.91 N),followed by Medicago(68.07N)and Morus(61.88 N),and the average root tensile strength showed the same trend,16.52 MPa for Zanthoxylum,16.08 MPa for Medicago and 13.02MPa for Morus.(3)The bio-embankment measures significantly improved the soil shear resistance,especially under vertical loads of 1oo kPa and *** soil internal friction angle showed a significant log-positive correlation with root morphological parameters of root length density(RLD),root surface area density(RSAD) and rootweight density(RWD),while the soil cohesion force showed a positive linear correlation with these *** results provide effective parameters supporting for the design of bio-embankm

关键词： Bio-embankments Root distribution Tensile properties Shear strength Earth bank collapse Purple hilly area

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Petrology and Geochemistry of Basalts from Tonnge Area, Tigyaing Township, Sagaing Region, Myanmar

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Open Journal of Geology 2019年第9期9卷 516-526页

作者： Myo Min Tun Myo Theingi san Yee Khaing Thet Naing Zar oo sann Aung Kyaw Thin Department of Geology Yadanabon University Mandalay Myanmar Department of Geology Loikaw University Loikaw Myanmar Department of Earth Resources Engineering Kyushu University Fukuoka Japan Department of Geology Pathein University Pathein Myanmar Department of Geology Defence Services Technological Academy Pyin Oo Lwin Myanmar Banmaw University Banmaw Myanmar

Tonnge basalt is a relatively small basaltic field situated in the Tigyaing Township, Sagaing Region, Myanmar. This basaltic field is considered as one of the occurrences of younger extrusive rocks along the major strike-slip fault of central Myanmar. The basaltic lava flows erupted and rested upon the Upper Miocene-Pliocene Irrawaddy sandstone. Petrological and geochemical studies of the Tonnge basalts have not been carried out yet. This paper reports the results of petrological and geochemical investigation on the basalts from Tonnge area. These data have provided insight into the origin and petrogenetic processes during evolution. The petrographic characteristics of the basalts have been studied using polarizing microscope. The major- and trace-element compositions of basalt samples were analyzed using X-Ray Fluorescence. The Tonnge basalts are porphyritic, holocrystalline and consist of phenocrysts of olivine, plagioclase ± clinopyroxene which are set in the fine-grained intergranular groundmass of olivine, pyroxene, and opaque minerals. The basalts show typical characters of alkali olivine basalts. Trace element assemblage of the studied basalts plots on various discrimination diagrams as within-plate basalt erupted in the continental setting. A relatively small volume, monogenetic nature and unique geochemical characteristics of the basaltic lavas suggest that they are derived from a single, short-lived, discrete parental magma as a result of partial melting.

关键词： Tonnge Olivine Basalt Within-Plate Partial Melting

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基于统一建模语言的电子政务研究

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情报科学 2007年第4期25卷 592-598页

作者：王昊邓三鸿南京大学信息管理系江苏南京210093

本文主要介绍面向对象技术中统一建模语言(UML)在电子政务系统建模开发中的应用。论述电子政务(E-Government)的内涵、体系模型的构成以及实现系统的关键技术;概述适用于电子政务系统建模的统一建模语言的建模机制、建模过程和具体内容... 详细信息

本文主要介绍面向对象技术中统一建模语言(UML)在电子政务系统建模开发中的应用。论述电子政务(E-Government)的内涵、体系模型的构成以及实现系统的关键技术;概述适用于电子政务系统建模的统一建模语言的建模机制、建模过程和具体内容;重点讨论统一建模语言在电子政务系统需求分析、系统分析、系统设计、系统实现以及系统部署等5个阶段中的具体应用;最后展望今后电子政务实现领域和面向对象UML技术结合课题的研究重点和发展方向。

关键词：电子政务统一建模语言建模 UNL图增量迭代

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Na_2Moo_4催化H_2O_2氧化芥子气模拟剂苯甲硫醚的动力学与产物

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分子催化 2015年第1期29卷 45-51页

作者：赵三平习海玲左言军王琦国民核生化灾害防护国家重点实验室北京102205 防化研究院北京102205

以苯甲硫醚(Ph SMe)为化学毒剂芥子气的模拟剂,研究了Na2Mo O4催化H2O2溶液氧化Ph SMe的两阶段反应动力学及产物分布.Ph SMe在Na2Mo O4催化H2O2溶液中生成了苯甲亚砜(Ph S(O)Me)和苯甲砜(Ph S(O)2Me).相对无催化H2O2溶液,Na2Mo O4催化... 详细信息

以苯甲硫醚(Ph SMe)为化学毒剂芥子气的模拟剂,研究了Na2Mo O4催化H2O2溶液氧化Ph SMe的两阶段反应动力学及产物分布.Ph SMe在Na2Mo O4催化H2O2溶液中生成了苯甲亚砜(Ph S(O)Me)和苯甲砜(Ph S(O)2Me).相对无催化H2O2溶液,Na2Mo O4催化在加快Ph SMe氧化为Ph S(O)Me的同时,也将过度氧化产物Ph S(O)2Me的产率提高了两个数量级.95Mo NMR测试表明,Mo O42-与H2O2反应生成了钼酸盐过氧配合物Mo Ox(oo)(4-x)2-,当H2O2/Mo O42->5时,Mo(O2)42-是主要的活性物种,通过直接氧转移方式导致硫醚→亚砜→砜的氧化.

关键词：钼酸钠过氧化氢芥子气苯甲硫醚 95Mo NMR

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