检索结果-南通市图书馆

Distributed Differences Structures Underlie Gating between the Kin Channel KAT1 and the Kout Channel SKOR

Molecular Plant 2010年第1期3卷 236-245页

作者： Janin Riedelsberger Tripti Sharma Wendy Gonzalez Pawel Gajdanowicz samuel Elias Morales-navarro Carlos Garcia-Mata Bernd Mueller-Roeber Fernando Danilo Gonzalez-nilo Michael R. Blatt Ingo Dreyer Universitat Potsdam Institut for Biochemie und Biologie Molekularbiologie Heisenberg-Gruppe Biophysik und Molekulare Pflanzenbiologie BPMPB Karl-Liebknecht-Strasse 24-25 Haus 20 D-14476 Potsdam-Golm Germany Max-Planck Institute of Molecular Plant Physiology 14476 Potsdam-Golm Germany Centro de Bioinformatica y Simulacion Molecular Universidad de Talca Casilla 721 Talca Chile Laboratory of Plant Physiology and Biophysics IBLS Plant Sciences Bower Building University of Glasgow Glasgow G12 8QQ UK Laboratorio de Fisiologia Molecular e Integrativa Institutos de Investigaciones Biologicas Universidad Nacional de Mar del Plata 7600 Mar del Plata Buenos Aires Argentina Universitat Potsdam Institut for Biochemie und Biologie Abteilung Molekularbiologie 14476 Potsdam-Golm Germany

The family of voltage-gated （Shaker-like） potassium channels in plants includes both inward-rectifying （Kin） channels that allow plant cells to accumulate K＋ and outward-rectifying （Kout） channels that mediate K＋ efflux. Despite their dose structural similarities, Kin and Kout channels differ in their gating sensitivity towards voltage and the extracellular K＋ concentration. We have carried out a systematic program of domain swapping between the Kout channel SKOR and the Kin channel KAT1 to examine the impacts on gating of the pore regions, the S4, S5, and the S6 helices. We found that, in particular, the n-terminal part of the S5 played a critical role in KAT1 and SKOR gating. Our findings were supported by molecular dynamics of KAT1 and SKOR homology models. In silico analysis revealed that during channel opening and closing, displacement of certain residues, especially in the S5 and S6 segments, is more pronounced in KAT1 than in SKOR. From our analysis of the S4-S6 region, we conclude that gating （and K＋-sensing in SKOR） depend on a number of structural elements that are dispersed over this -145-residue sequence and that these place additional constraints on configurational rearrangement of the channels during gating.

关键词： Arabidopsis K+ channel outward rectifier inward rectifier channel protein structure channel protein-cation interaction gating, K+-dependent.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Prophylactic tracheal intubation for upper GI bleeding: A meta-analysis

引用

World Journal of Meta-Analysis 2015年第1期3卷 4-10页

作者： Ashraf A Almashhrawi Rubayat Rahman samuel T Jersak Akwi W Asombang Alisha M Hinds Hazem T Hammad Douglas L nguyen Matthew L Bechtold Division of Gastroenterology and Hepa-tology University of Missouri Columbia MO 65212 United States Division of Gastroenterology and Hepa-tology University of California Irvine CA 92697 United States

AIM： To evaluate usefulness of prophylactically intu-bating upper gastrointestinal bleeding （UGIB） patients. METHODS： UGIB results in a significant number of hospital admissions annually with endoscopy being the key intervention. In these patients, risks are associated with the bleeding and the procedure, including pulmonary aspiration. However, very little literature is available assessing the use of prophylactic endotracheal intubation on aspiration in these patients. A comprehensive search was performed in May 2014 in Scopus, CInAHL, Cochrane databases, PubMed/Medline, Embase, and published abstracts from national gastroenterology meetings in the United States （2004-2014）. Included studies examined UGIB patients and compared prophylactic intubation to no intubation before endoscopy. Meta-analysis was conducted using RevMan 5.2 by Mantel-Haenszel and DerSimonian and Laird models with results presented as odds ratio for aspiration, pneumonia （within 48 h）, and mortality. Funnel plots were utilized for publication bias and I2 measure of inconsistency for heterogeneity assessments. RESULTS： Initial search identi？ed 571 articles. Of these articles, 10 relevant peer-reviewed articles in English and two relevant abstracts were selected to review by two independent authors （Almashhrawi AA and Bechtold ML）. Of these studies, eight were excluded： Five did not have a control arm, one was a letter the editor, one was a survey study, and one was focused on prevention of UGIB. Therefore, four studies （n = 367） were included. Of the UGIB patients prophylactically intubated before endoscopy, pneumonia （within 48 h） was identified in 20 of 134 （14.9%） patients as compared to 5 of 95 （5.3%） patients that were not intubated prophylactically （P = 0.02）. Despite observed trends, no significant differences were found for mortality （P = 0.18） or aspiration （ P = 0.11）.COnCLUSIOn： Pneumonia within 48 h is more likely in UGIB patients who received prophylactic endotracheal intubation p

关键词： Prophylactic endotracheal intubation Upper gastrointestinal bleeding Endoscopy Complication Pneumonia Aspiration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Experience of, and Perception on Gender Based Violence (GBV) by Pregnant Women in South Eastern nigeria

引用

Advances in Reproductive Sciences 2019年第4期7卷 113-124页

作者： Joseph Ifeanyi Brian-D. Adinma Victor nwabunwanne Oguaka Joseph Odiri Ugbaja Osita samuel Umeononihu nneoma Dolores Adinma-Obiajulu Odira Linda Okeke Department of Obstetrics and Gynaecology Nnamdi Azikiwe University and Teaching Hospital Nnewi Nigeria Department of nursing Sciences Nnamdi Azikiwe University and Teaching Hospital Nnewi Nigeria

Background: Gender-based violence (GBV) is a common women’s sexual and reproductive right violation. Pregnancy could be associated with an increased risk of GBV. Objective: To determine the experience of, and perception on GBV by pregnant nigerian women. Methodology: A questionnaire-based cross-sectional study involving 250 pregnant women from six selected health facilities in Anambra, Southeastern nigeria was conducted to elicit their experience of, and perceptions on gender based violence. Data analysis employed statistical package for social sciences (SPSS) version 22.0. Statistical comparison of variables employed Chi-square test, with significance levels comprising of p Results: Only 80 (32.0%) of the respondents were aware of GBV while 17 (6.8%) and 20 (8.0%) had experienced it in the index and previous pregnancies respectively. Physical violence was the most commonly experienced 18 (58.1%), while marital quarrel and drunkenness were the most commonly perceived causes, 21 (26.3%) apiece. Up to 33.7% of the respondents would report GBV to the law enforcement agencies;22.5% would seek medical attention and only 2.5% would do nothing. The most common source of information on GBV was public lecture (32.5%) followed by media (26.3%). As high as 49 (61.3%) of the respondents that are aware of GBV (n = 80), thought that GBV could be prevented while up to 53 (66.3%) were aware of the strategies for its prevention. Conclusion: Awareness of GBV in this study is low (32.0%) while experience is even lower at 6.8%, and 8.0% for the index and previous pregnancies respectively. Information on prevention strategies for GBV is also low. The need to develop comprehensive reproductive health (RH) policy that incorporates GBV and its management, to be taught in educational institutions is highlighted. The importance of public health lectures and the employment of various media platforms as reproductive right enlightenment modalities, towards creating awareness on the prevention a

关键词： Experience Perception Gender Based Violence Pregnant Women Southeastern nigeria

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Semi-Automated Method for Multi-Tasking Measurement of Microbial Growth, Capsule, and Biofilm Formation

引用

Advances in Microbiology 2012年第4期2卷 623-628页

作者： Derek T. Kirby Craigory J. Raino samuel F. Rabor Jr. Cassandra J. Wasson Balbina J. Plotkin Department of Microbiology and Immunology Midwestern University Downers Grove USA Department of Microbiology and Immunology Midwestern University Downers Grove USA

The majority of bacterial infections involve the formation of biofilms. Biofilm formation is nutrient and growth dependent. Determination of the effects of nutrients on exopolysaccharide production and bacterial growth is labor and time intensive. We tested whether the Bioscreen C (Growth Curves, Inc.) would have utility as a high-throughput tool in the measurement of fundamental phenotype expression, as it relates to growth conditions. Within 48 - 72 hr, reproduceble, statistically significant data on the affects of growth conditions on generation time, capsule production and biofilm formation (maximally for 25 different conditions per 24 hr run cycle;n = 4) were obtained. Although all S. aureus strains produced similar amounts of capsule, sarA– and agr– strains grew significantly slower than parent strain (1.6 fold slower) and produced significantly (p 0.05) less biofilm (~2 fold). E. coli growth rate, biofilm and capsule production in simulated nephropathic urine medium was similar for urine with insulin (20 μU). Addition of insulin to urine medium with proline increased generation time, capsule and biofilm production. Findings from this study show that the Bioscreen C is a rapid, reproducible, and easily manipulated system to concurrently measure bacterial growth, biofilm formation, and capsule production. In addition, there is the potential for further applications of this system by expanding the types of detector dyes used.

关键词： Biofilm Staphylococcus aureus Escherichia coli Quorum-Signaling Compounds Menaquinone Insulin Growth

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

利用腔静脉减影二维相位对比MR成像测量肝总血流量和肝动脉血流量:临床前验证和初步临床过渡

引用

国际医学放射学杂志 2016年第6期 683-684页

作者： M.D.Chouhan R.P.Mookerjee A.Bainbridge S.Walker-samuel n.Davies S.Halligan 胡伟劼 University College London Centre for Medical Imaging Division of MedicineUniversity College London

摘要目的在动物模型中,验证利用腔静脉减影二维相位对比MR成像测定肝总血流量和肝动脉血流量的方法,并评估其在人体应用中的一致性与可重复性。方法动物保护

关键词：血流量相位对比 MR 减影腔静脉肝动脉临床临诊

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

用杀虫剂防治咖啡苗圃蛴螬的效果

引用

世界热带农业信息 1985年第5期 50-51页

作者： S. D. samuel 林延谋

鞘翅目鳃角金龟科有数种鳃金龟(Holotrichia)的白蛴螬为害咖啡根部,对苗圃和新植区的幼龄咖啡为害更严重。已推荐的防治白蛴螬的药剂有甲拌磷、呋喃丹和林丹。最近的研究结果表明,用异丙胺磷防治苗圃白蛴螬效果良好。本研究目的是评价... 详细信息

鞘翅目鳃角金龟科有数种鳃金龟(Holotrichia)的白蛴螬为害咖啡根部,对苗圃和新植区的幼龄咖啡为害更严重。已推荐的防治白蛴螬的药剂有甲拌磷、呋喃丹和林丹。最近的研究结果表明,用异丙胺磷防治苗圃白蛴螬效果良好。本研究目的是评价四种颗粒杀虫药剂防治蛴螬的效果。

关键词：剂量用药量稻丰散有机氮杀虫剂西维因 0-(1-萘基)-n-甲基氨基甲酸酯苗圃咖啡

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

大学如何支持留学生跨文化适应——基于美国n大学实践的参与式观察

引用

黄冈师范学院学报 2019年第4期39卷 86-89页

作者：杨桔 samuel H.Song 黄蕙萍皖西学院经济与管理学院安徽六安237012 nazareth College English Language Institute Office for Asia Pacific Rim InitiativesNYPittsford 14618 武汉理工大学经济学院湖北武汉430070

跨文化认同对于留学生的规模有显著的促进作用,跨文化认同程度的提升对留学生人数会产生正向效应。因此,从教育经济学的角度看,非常有必要研究留学生的跨文化认同问题。本文采用参与式观察的研究方法,基于社会网络理论,以观察者和参与... 详细信息

跨文化认同对于留学生的规模有显著的促进作用,跨文化认同程度的提升对留学生人数会产生正向效应。因此,从教育经济学的角度看,非常有必要研究留学生的跨文化认同问题。本文采用参与式观察的研究方法,基于社会网络理论,以观察者和参与者的双重身份,对大学支持留学生跨文化适应的各项具体措施进行分析。基于案例分析,探索跨文化适应支持措施的内在普遍性,从而为中国高校如何帮助来华留学生实现跨文化适应提供借鉴。

关键词：留学生跨文化适应社会网络参与式观察

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Active tracking of rejected dried blood samples in a large program in nigeria

引用

World Journal of Virology 2016年第2期5卷 73-81页

作者： Auchi Inalegwu Sunny Phillips Rawlings Datir Christopher Chime Petronilla Ozumba samuel Peters Obinna Ogbanufe Charles Mensah Alash'Le Abimiku Patrick Dakum nicaise ndembi Institute of Human Virology US Centers for Disease Control and Prevention Embassy of the United States of America Institute of Human Virology University of Maryland School of Medicine

AIM: To study the impact of rejection at different levels of health care by retrospectively reviewing records of dried blood spot samples received at the molecular laboratory for human immunodeficiency virus(HIV) early infant diagnosis(EID) between January 2008 and December ***: The specimen rejection rate, reasons for rejection and the impact of rejection at different levels of health care was examined. The extracted data were cleaned and checked for consistency and then deduplicated using the unique patient and clinic identifiers. The cleaned data were ciphered and exported to SPSS version 19(SPSS 2010 IBM Corp, new York, United States) for statistical ***: Sample rejection rate of 2.4%(n = 786/32552) and repeat rate of 8.8%(n = 69/786) were established. The mean age of infants presenting for first HIV molecular test among accepted valid samples was 17.83 wk(95%CI: 17.65-18.01) vs 20.30 wk(95%CI: 16.53-24.06) for repeated samples. HIV infection rate was 9.8% vs 15.9% for accepted and repeated samples. Compared to tertiary healthcare clinics, secondary and primary clinics had twofold and three-fold higher likelihood of sample rejection, respectively(P nificant increase in sample rejection rate with increasing number of EID clinics(r = 0.893, P = 0.041). The major reasons for rejection were improper sample collection(26.3%), improper labeling(16.4%) and insufficient blood(14.8%). COnCLUSIOn: Programs should monitor pre-analytical variables and incorporate continuous quality improvement interventions to reduce errors associated with sample rejection and improve patient retention.

关键词： Human immunodeficiency virus Prevention of mother-to-child transmission Early infant diagnosis Dried blood spot Pre-analytical error Sample rejection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

High Supracostal Percutaneous nephrolithotomy Access: Assessing Safety in Access above the Eleventh Rib after Performing Preoperative Planning with Computed Tomography

引用

Open Journal of Urology 2015年第4期5卷 25-33页

作者： Joel E. Abbott Anthony D. DiMatteo Elise Fazio samuel G. Deem Ali K. Sobh Albert DePolo Julio G. Davalos St John Providence Health Michigan State University Warren USA Charleston Area Medical Center West Virginia School of Osteopathic Medicine Charleston USA Chesapeake Urology Associates University of Maryland Glen Burnie USA

Objective: To determine if supracostal renal access above the 11th rib during percutaneous nephrolithotomy (PCnL) is a safe option in carefully selected patients determined by preoperative computed tomography (CT) imaging. Patients and Methods: We retrospectively isolated 142 patients who underwent access above the eleventh rib during PCnL, which we term “high supracostal renal access.” We then compared these patients to 113 individuals who underwent access below the twelfth rib. Renal access was achieved by the operative surgeon with fluoroscopic guidance in conjunction with pre-operative computed tomography (CT) scan. Outcomes were compared. Results: Overall surgical outcomes were equivalent when comparing high supracostal versus subcostal access sites. As expected due to proximity, pleural complications occurred in 4% of the high supracostal group (n = 6) compared with 0% of the control (subcostal) group (p = 0.035). Of these six complications, three were managed conservatively with observation and two required cardio-thoracic intervention with video-assisted thoracoscopic pleural repair (1%). In the remaining case, the patient was preoperatively consented for placement of a thoracostomy tube, which was placed during the procedure, due to the difficult location of her upper pole stone and closely adjacent low-lying pleura, and the planned transpleural approach. Hospital stay was not significantly pro-longed between the high supracostal access and subcostal access groups, with an average length of stay of 2.2 ± 2.1 days and 2.0 ± 1.9 days (p = 0.59) respectively. Conclusions: Careful, systematic preoperative planning based on CT and fluoroscopic imaging allows for a confident understanding of a “safety zone” in placement and dilatation of renal access points during PCnL. We have shown that planned upper pole renal access above the 11th rib is achievable with acceptable morbidity and excellent success rates.

关键词： Percutaneous nephrolithotomy Calculi Endoscopic Surgical Procedure

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of coat colour genes on body measurements, heat tolerance traits and haematological parameters in West African Dwarf sheep

引用

Open Journal of Genetics 2013年第4期3卷 280-284页

作者： John S. Decampos Christian O. n. Ikeobi Olajide Olowofeso Olusiji F. Smith Matthew A. Adeleke Mathew Wheto David O. Ogunlakin Abubakar A. Mohammed Timothy M. Sanni Babatunde A. Ogunfuye Raman A. Lawal Adeyemi S. Adenaike samuel A. Amusan Department of Animal Breeding and Genetics Federal University of Agriculture Abeokuta Nigeria Department of Animal Physiology Federal University of Agriculture Abeokuta Nigeria

With 178 West African Dwarf sheep aged 1 to 3 years, a study was conducted to investigate the effects of coat colour genes on body measurements, heat tolerance traits and haematological parameters. Body measurements considered included body length, hair length, ear length, hip width, tail length, height at withers, rump height, fore cannon bone length, chest depth, heart girth and body weight. Heat tolerance traits considered were skin temperature, rectal temperature, pulse rate and respiratory rate. Blood samples were collected for the evaluation of white blood cell (WBC), red blood cell (RBC), haemoglobin (HGB), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin cell (MCHC), red cell distribution width (RDW), platelets (PLT), mean platelets volume (MPV), platelets distribution width (PDW) and plateletcrits (PCT). Results showed that coat colour gene (CCG) had significant (P n rump height and tail length. Animals with black (BB) coat colour had the highest mean value for rump height (57.80 ± 1.29 cm) and tail length (22.10 ± 0.89 cm), while brown (Bb) coat colour had the least value of 53.00 ± 6.00 cm for rump height and 17.50 ± 0.50 cm for tail length. The CCG had significant (P n body temperature and pulse rate, with the grey/mouflon (Ag) colour possessing the highest body temperature (38.90°C ± 0.22°C), and Bb having the least value of 37.20°C ± 0.35°C. White/tan (Awt) had the highest pulse rate of 28.90 ± 0.66 beats/min and Bb had the least value of 20.00 ± 2.00 beats/min. The CCG had significant (P n RBC and MPV with brown (Bb) colour having the highest RBC counts (18.20 ± 0.00 L) and badgerface (Ab) having the least value (11.50 ± 0.62 L). The Bb had the highest value (5.60 ± 0.00 fL) for MPV and Ab had the least value (4.70 ± 0.15 fL). Sheep with Bb and Ab were found to withstand heat stress better than others.

关键词： Coat Colour Gene Haematological Parameters Morphological Indices Sheep

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：