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Study on PVL, blaOXA-23 and blaOXA-51 Genes in Drug Resistant Staphylococcus aureus Causing Surgical-Sites and Traumatic Wounds Infections, Sudan

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American Journal of Molecular Biology 2022年第2期12卷 30-42页

作者： Sanaa Mohammed Yousif Adam Dawoud Abakar Salaheldein Gumaa Elzaki salma Omer Ibrahim Omer Abu Elhasan Mohamed Taj-Eldin Elhadi Abdalla Ahmed National Blood Bank Ministry of Health Khartoum Sudan Department of Medical Parasitology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan Molecular Biology Laboratory Department of Epidemiology Tropical Medicine Research Institute Khartoum Sudan Department of Medical Microbiology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan Wad medani College of Medical Sciences and Technology Wad Medani Sudan

Background: The characteristics of Staphylococcus aureus that made it the most important cause of wound infections are environmental spread antimicrobials resistance and virulence. Absence of molecular detection of drug resistance and virulence factors in many developing countries limits the epidemiological information. This study conducted to identify PVL virulence gene, and blaOXA-23 and blaOXA-51 drug resistance genes of Staphylococcus aureus isolated from surgical-sites infections (SSIs) and traumatic wounds. Methods: A cross-sectional study was conducted from 2019 to 2021, in which 70 cefepime resistant Staphylococcus aureus were used, the strains were isolated from patients of SSIs and traumatic wounds admitted to the department of General Surgery in Wad medani Teaching Hospital. Mannitol salt agar was used for primary culture followed by biochemical identification and Kirby Bauer susceptibility testing. Single and multiplex PCR protocols performed for bacterial confirmation and target genes detection. Results: Staphylococcus aureus strains from SSIs constituted 56% (39/70) from which 41% (16/39) possessed PVL gene while 42% (13/31) of wound infections strains were positive for PVL gene. Presence of PVL gene was significantly associated with resistance to meropenem (P. value 0.023) and ceftriaxone (P. value 0.037). blaOXA-23 was significantly detected with resistance to meropenem, augmentin and ceftriaxone. While blaOXA-51 was significantly identified among Staphylococcus aureus strains that showed resistance to meropenem and ciprofloxacin. Conclusion: This is the first study in Sudan that identified blaOXA-23 and blaOXA-51 in Staphylococcus aureus and correlated them to resistance to commonly used antimicrobials. Meropenem resistant Staphylococcus aureus were significantly positive for PVL, blaOXA-23 and baOXA-51 genes.

关键词： Staphylococcus aureus PVL blaOXA-23 blaOXA-51 Wounds Sudan

Urosepsis among Sudanese Patients: A Paradigm from Limited Resources Country

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Advances in Microbiology 2022年第3期12卷 109-120页

作者： salma Omer Ibrahim Mohammed Elimam Sami Mahjoub Taha Sanaa Mohammed Yousif Hajir Mohammed Hussein Omer Mirghani Yousif Seitelbanat Yassin Ali Elnaiem Yousif Abdelhameed Mohammed Omer Abu Elhasan Adama Dawoud Abakar Elhadi Abdalla Ahmed Department of Medical Microbiology Faculty of Medical Lab Sciences University of Gezira Wad Medani Sudan Faculty of Medicine University of Gezira Wad Medani Sudan National Blood Bank Ministry of Health Khartoum Sudan Clinical Pharmacy and Pharmacy Practice Faculty of Pharmacy University of Gezira Wad Medani Sudan Department of Pharmaceutics Faculty of Pharmacy University of Gezira Wad Medani Sudan Resident at Gezira Hospital for Renal Disease and Surgery Urology (GHRDS) Wad Medani Sudan Cancer National Institute University of Gezira Wad Medani Sudan Wad Madani College for Medical Science and Technology Wad Medani Sudan Department of Medical Parasitology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan

Background: Urosepsis is life-threatening sepsis that leads to organ dysfunction and results from a defective response to a urinary tract infection;the major precipitating is obstructive uropathy in the upper or lower urinary tract (UT). The magnitude and burden of bacteria that caused uropathy were reported to increase annually. In 30% of all septic patients who were diagnosed with urosepsis, 1.5% of them were found in urology and a quarter due to hospital-acquired urinary tract infections (HAUTIs). This study aims to determine the clinical pattern and the frequency of commonly used antibiotics against bacteria associated with urosepsis among Sudanese patients. Methods: This was a cross sectional laboratory-based study, study subjects were recruited from patients attended to Gezira Hospital for Renal diseases and surgery (GHRDS) and was diagnosed, on clinical and laboratory basis, to have urosepsis. Hundred (n = 100) urine samples were collected and inoculated on cysteine lactose electrolyte deficient agar (CLED) media and identify using the suitable biochemical test and performed antimicrobial susceptibility testing (AST) by Kirby Bauer disc diffusion technique for selected antimicrobial agents, according to clinical laboratory standard institute (CLSI) guidelines. Results: Amongst urosepsis infection the frequency of E. coli, S. aureus, Proteus mirabilis, Klebseilla pneumonia and Pseudomonas aeruginosa were (37%, 21%, 10%, 6%, 4% respectively). Resistance to commonly prescribed antibiotics was high, ranging from 17% for meropenem to 100% for cefepime. P. aeruginosa was multidrug resistant compared with other isolates. Conclusions: There was high rate of antibiotic resistance against the common causes of urosepsis in GHRDS, and this reflects the importance of culture and sensitivity test and necessitates adoption of guidelines for selection of suitable antibiotic.

关键词： Urosepsis Antimicrobial Susceptibility Gezira Hospital for Renal Diseases and Surgery Sudan

Frequency and Antimicrobials Susceptibility Pattern of <i>Staphylococcus aureus</i>Associated with Wound Infections in Surgery Department, Wad Madani Teaching Hospital, Sudan

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Pharmacology & Pharmacy 2021年第12期12卷 334-343页

作者： Sanaa M. Yousif Adam D. Abakar Bakri Y. M. Nour salma O. Ibrahim Omer M. Abu Elhasan Mirgani A. Yousif Sitelbanat Yassin Hajer M. Hussien Mohamed Taj-Eldin Elhadi A. Ahmed National Blood Bank Ministry of Health Khartoum Sudan Department of Medical Parasitology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan Department of Medical Microbiology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan Wad-Madani College of Medical Sciences and Technology Wad Medani Sudan Faculty of Pharmacy University of Gezira Wad Medani Sudan

Background: Most community and hospital acquired infections are due to Staphylococcus aureus, these infections involve both local suppurative and systemic conditions. There is an increase in the incidence rates of acquiring resistant strains of Staphylococcus aureus in hospitals and community in Sudan. This study aims to detect frequency of Staphylococcus aureus isolated from surgical-sites infections (SSIs) and traumatic wounds, and to determine susceptibility to commonly used drugs. Methods: Prospective cross-sectional laboratory based study was followed, 250 patients admitted to Surgery Departments at Wad Madani Teaching Hospitals during 2019 and 2020 were recruited;with clinical manifestation of SSIs and or/traumatic wounds. Swab sample was taken from each participant and incubated aerobically on mannitol salt agar. Biochemical tests and 16rRNA gene used for Staphylococcus aureus identification. Antimicrobial susceptibility was accomplished using Kirby Bauer disc diffusion technique. Results: Infection rate of wounds in males and females equal 71% (17/250) and 29% (73/250), while infection rate in the age group of 41 to 60 y

关键词： Staphylococcus aureus Surgical-Sites Wounds Meropeneme Sudan

Incidence of Oxa23 and Oxa51 Genes Associated with Bacterial Isolated from Patients with Urosepsis: Single Centre Prespective

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American Journal of Molecular Biology 2022年第3期12卷 85-96页

作者： salma Omer Ibrahim Elimam M. A. Mohammed Sami Mahjoub Taha Sanaa Mohammed Yousif Hajir Omer Omer Omer Mirghani Seif-Elnasr Seitelbanat Yassin Yousif Abdelhameed Mohammed Omer Abu Elhasan Mohamed Taj-Eldin Adam Dawoud Abakar Elhadi Abdalla Ahmed Salaheldein G. Elzaki Department of Medical Microbiology Faculty of Medical Lab Sciences University of Gezira Wad Medani Sudan Department of Urology Faculty of Medicine University of Gezira Wad Medani Sudan National Blood Bank Ministry of Health Khartoum Sudan University Hospital Galway Galway Ireland Department of Urology Gezira Hospital for Renal Disease and Surgery Wad Medani Sudan Department of Pharmaceutics Faculty of Pharmacy University of Gezira Wad Medani Sudan Department of Clinical Chemistry National Cancer Institute University of Gezira Wad Medani Sudan Department of Medical Microbiology Wad Medani College for Medical Science and Technology Wad Medani Sudan Department of Medical Parasitology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan Department of Epidemiology Tropical Medicine Research Institute Khartoum Sudan

Background: Urosepsis is one of the most common infections that require empirical broad spectrum antibiotics immediately after diagnosis. This has led to development of bacterial resistance by acquiring the capability to destroy the β-lactam ring. Methodology: This is a cross-sectional hospital-based study. The study was conducted from 2019 to 2020 at Gezira Hospital for Renal diseases and surgery (GHRDS). A hundred patients were diagnosed clinically with urosepsis and the isolated organisms were Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumonia and Pseudomonas aeruginosa. The susceptibility test was conducted by Kirby Bauer disc diffusion technique according to clinical laboratory standard institute (CLSI) guidelines. Seventy eight samples of bacterial genomic DNA were confirmed by 16srRNA and multiplex PCR, were performed for genotypic blaOXA-51 and blaOXA-23 gene characterization of isolated bacteria. Then gel electrophoresis was used to identify the presence or absence of (blaOXA-51 and blaOXA-23) genes. Results: 88.5% (69/78) in 16srRNA detected. Using multiplex PCR, the frequencies of blaOXA-51 and blaOXA-23 genes were 13% and 10.1%, respectively. The percentages of isolates which yielded both blaOXA-51 and blaOXA-23 among P. aeruginosa was 25% (1/4), among K. pneumonia was 17% (1/6), and among E. coli was 8% (3/37). Only blaOXA-51 was detected in P. mirabilis 10% (1/10) and only blaOXA-23 was detected in S. aureus 5% (1/18). Conclusion: In this study, the presence of blaOXA-51 and blaOXA-23 genes was increased in the isolated bacteria.

关键词： Urosepsis Carbapenem-Resistant Enterobacteriaceae (CRE) blaOXA-51 and blaOXA-23