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Alteration of chemical behavior of L-ascorbic acid in combination with nickel sulfate at different pH solutions in vitro

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Asian Pacific Journal of Tropical Biomedicine 2012年第3期2卷 220-222页

作者： Shaheen A Maniyar Jameel G Jargar Swastika N Das salim a dhundasi Kusal K Das Environmental Health Research Unit Department of PhysiologyAl Aineen Medical College Department of Engineering Chemistry B.L.D.E.A's V.P.Dr.P.G.Halakatti College of Engineering and Technology

Objective:To evaluate the alteration of chemical behavior of L-ascorbic acid(vitamin C) with metal ion(nickel) at different pH solutions in ***:Spectra of pure aqueous solution of L-ascorbic acid(E mark) compound and NiSO_4(H_2O)(sigma USA) were evaluated by UV visible *** analysis of L-ascorbic acid and nickel at various pH(2.0, 7.0,7.4 and 8.6) at room temperature of 29℃ was *** this special analysis,combined solution of L-ascorbic acid and nickel sulfate at different pH was also ***:The result revealed that λ_(max)(peak wavelength of spectra) of L-ascorbic acid at pH 2.0 was 289.0 run whereas at neutral pH 7.0,λ_(max) was 29S.4 *** alkaline pH 8.6,λ_(max) was 295.4 nm and at pH 7.4 the λ_(max) of L-ascorbic acid remained the same as 295.4 *** solution at acidic pH 2.0 was 394.5 nm,whereas at neutral pH 7.0 and pH 7.4 were the same as 394.5 *** at alkaline pH 8.6,λ_(max) value of nickel sulfate became 392.0 *** combined solution of L-ascorbic acid and nickel sulfate(6 mg/mL each) at pH 2.0 showed 292.5 nm and 392.5 nm,respectively whereas at pH 7.0,L-ascorbic acid showed 296.5 nm and nickel sulfate showed 391.5 *** pH 7.4,L-ascorbic acid showed 297.0 nm and nickel sulfate showed 394.0 nm in the combined solution whereas at pH 8.6(alkaline) L-ascorbic acid and nickel sulfate were showing 297.0 and 393.5 nm,respectively. Conclusions:Results clearly indicate an altered chemical behavior of L-ascorbic acid either alone or in combination with nickel sulfate in vitro at different *** oxidation of L-ascorbic acid to L-dehydro ascorbic acid via the free radical(HSc*) generation from the reaction of H,ASc + Ni(Ⅱ) is the cause of such alteration of λ_(max),value of L-ascorbic acid in the presence of metal nickel.

关键词： L-ascorbic acid Nickel pH Spectrum Chemical behavior Spectra UV visible spectrophotometer Peak wavelength Free radical Vitamin C Nickel sulfate

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