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micropropagation of Daylily(Hemerocallis fulva)from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT markers

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Phyton-International Journal of Experimental Botany 2023年第7期92卷 2183-2196页

作者： Esraa E.Shalan Said S.Soliman Ahmed A.mahmoud Jameel m.Al-Khayri salha m.alshamrani Fatmah A.Safhi Areej S.Jalal Diaa Abd El-moneim Abdallah A.Hassanin Genetics Department Faculty of AgricultureZagazig UniversityZagazig44511Egypt Department of Agricultural Biotechnology College of Agriculture and Food SciencesKing Faisal UniversityAl-Ahsa31982Saudi Arabia Department of Biology College of ScienceUniversity of JeddahJeddah21959Saudi Arabia Department of Biology College of SciencePrincess Nourah bint Abdulrahman UniversityRiyadh11671Saudi Arabia Department of Plant Production (Genetic Branch)Faculty of Environmental and Agricultural SciencesArish UniversityEl-Arish45511Egypt

Determination of the somaclonal variation of in vitro-propagated plants is crucial to determine the appropriate micropropagation protocol and growth regulators for commercial scale *** this research,nine multiplication media(mm)augmented with different concentrations of 6-benzyl adenine(BA),Kinetin(Kin),and Thidiazuron(TDZ),Three rooting media(Rm)supplemented with three levels ofα-naphthalene acetic acid(NAA)and three types of soil mixtures(v/v);Coco peat/Vermiculite/Sand(CVS),Peat moss/Perlite/Sand(PPS)and Peat moss/Perlite(PP)were used in the micropropagation protocol of daylily ***2 showed the maximum shoot length and the number of leaves,while mm9 showed the maximum number of *** Rm1 showed the maximum root length and the number of *** acclimatization,CVS,PPS,and PP soil mixture showed similar performance except the CVS mixture showed lower performance regarding plant height and *** genetic fidelity of micropropagated plants was evaluated using Start Codon Targeted(SCoT)*** SCoT primers amplified 51 scorable bands with an approximate range from 146 bp to 1598 bp *** one out of 51 loci were presented in the mother plants.40 loci were polymorphic,11 were monomorphic and 7 were *** amplification patterns of the micropropagated plants demonstrated genetic integrity to the mother plant ranging from 84.32 to 47.06 and somaclonal variations ranging from 52.94 with 5 mg/l BA pathway to 15.68 with 1mg/l TDZ pathway,thus demonstrating that the homogeneity and the variation of the micropropagated plants affected by the type and the quantity of the plant growth regulator used during multiplication *** research can be successfully used for other ornamental and medicinal plants’bulk multiplication,germplasm conservation,and future genetic improvement.

关键词： Genetic fidelity medicinal plants micropropagation molecular markers ornamental plants somaclonal variation

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Anatomical and molecular Identification of Ornamental Plant Ficus ***

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Phyton-International Journal of Experimental Botany 2023年第5期92卷 1329-1347页

作者： Abtisam Binnoubah Rim Hamdy Osama G.Ragab Ahmed m.El-Taher Ahmed Abou El-Yazied Fatmah A.Safhi Hala A.Elzilal Ashwaq T.Althobaiti salha m.alshamrani Diaa Abd El moneim Ahmed El-Banhawy Department of Botany Faculty of ScienceUniversity of SabrathaSabratha999116Libya Department of Botany Faculty of ScienceCairo UniversityGiza12111Egypt Department of Botany and microbiology Faculty of Science(Boys Branch)Al-Azhar UniversityCairo11884Egypt Department of Agricultural Botany Faculty of AgricultureAl-Azhar UniversityCairo11884Egypt Department of Horticulture Faculty of AgricultureAin Shams UniversityCairo11566Egypt Department of Biology College of SciencePrincess Nourah bint Abdulrahman UniversityRiyadh11671Saudi Arabia Department of Science and Technology College of RanyahTaif UniversityTaif21944Saudi Arabia Department of Biology College of ScienceTaif UniversityTaif21944Saudi Arabia Department of Biology College of ScienceUniversity of JeddahJeddah21959Saudi Arabia Department of Plant Production(Genetic Branch) Faculty of Environmental Agricultural SciencesArish UniversityEl-Arish45511Egypt Department of Botany and microbiology Faculty of ScienceSuez Canal UniversityIsmailia41522Egypt

This present study includes twelve species that represent the Ficus genus,namely;aspera,carica,tinctoria ***,hirta,hispida,neriifolia,palmata,pumila,racemosa,septica,sur,and sycomorus,belonging to the moraceae *** species samples were collected from various locations in *** study focused on the anatomical and molecular characteristics of mature foliage *** the identification and classification of taxa are highly dependent on the anatomical features of leaves,the anatomical characteristics were recorded in the form of a comparison between the examined plants in the data *** study aims to contribute to the identification of the studied species based on the anatomical details of the matured *** characterization includes the variations in upper and lower epidermal layers that are covered by a thin or thick cuticle;the number of palisade and spongy layers;crystals;secretory elements;lithocysts;the midrib zone has parenchyma associated with mechanical tissue,vascular system,and investigation of trichomes;on the other hand,in the current study,the phylogenetic analysis was conducted by using the ITS and 5.8 S *** the analysis of all the available data,it could be stated that there is an overall agreement with the anatomical character dendrogram.

关键词： ITS trichomes leaf anatomy Ficus moraceae

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Pre-Breeding Genetic Diversity Assessment of Tomato(Solanum lycopersicum L.)Cultivars Based on molecular,morphological and Physicochemical Parameters

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Phyton-International Journal of Experimental Botany 2023年第5期92卷 1493-1512页

作者： Jameel m.Al-Khayri salha m.alshamrani Adel A.Rezk Wael F.Shehata mustafa I.Almaghasla Tarek A.Shalaby Ahmed m.Saad Fatmah A.Safhi muhammad N.Sattar Arafat A.Abdel Latef mahmoud Z.Sitohy Abdallah A.Hassanin Department of Agricultural Biotechnology College of Agriculture and Food SciencesKing Faisal UniversityAl-Ahsa31982Saudi Arabia Department of Biology College of ScienceUniversity of JeddahJeddah21959Saudi Arabia Plant Pests and Diseases UnitDepartment of Arid Land AgricultureCollege of Agriculture and Food SciencesKing Faisal UniversityAl-Ahsa31982Saudi Arabia Department of Horticulture Faculty of AgricultureKafrelsheikh UniversityKafr El-Sheikh33516Egypt Department of Biochemistry Faculty of AgricultureZagazig UniversityZagazig44511Egypt Department of Biology College of SciencePrincess Nourah bint Abdulrahman UniversityP.O.Box 84428Riyadh11671Saudi Arabia Central Laboratories King Faisal UniversityAl-Ahsa31982Saudi Arabia Department of Botany and microbiology Faculty of ScienceSouth Valley UniversityQena83523Egypt Department of Genetics Faculty of AgricultureZagazig UniversityZagazig44511Egypt

Appropriate knowledge of the parental cultivars is a pre-requisite for a successful breeding *** study characterized fruit yield,quality attributes,and molecular variations of ten tomato cultivars during three consecutive generations under greenhouse *** 86,Castle Rock,and Red Star cultivars showed the highest fruit yield(kg/plant),total phenolic compounds(TPC),and sap *** component analysis categorized the evaluated fruit yield into three groups based on their quality attributes.A robust positive correlation appeared among traits inside each group.A positive correlation was likewise noticed between the first and the second ***,a negative correlation was detected between the first,the second and the third *** profiling,using seven inter-simple sequence repeat(ISSR)primers,produced 60 loci,including 49 polymorphic *** molecular analysis also pinpointed the highest genetic similarity(0.92)between P73 and moneymaker,while the lowest genetic similarity(0.46)was observed between Castle Rock and *** cultivars P73 and moneymaker showed the lowest genetic distance(2.24),while the highest genetic distance(5.92)was observed between Super marmand and Peto86,on the one hand,and between Castle Rock and moneymaker,on the other *** chemical analysis of fruit sap indicated the highest levels of TPC,total flavonoids,anthocyanin,ascorbic acid and total soluble solids in Peto 86 and Castle Rock *** analysis of tomato cultivars based on morphological and molecular attributes indicated four distinct *** 86,Castle Rock,and Red star cultivars can be recommended for the tomato hybridization breeding programs in the future,with other tomato cultivars as potentially high-yielding parents.

关键词： Fruit quality fruit yield genetic diversity hybrid seeds molecular markers plant breeding tomato

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Impact of multifocal gas-permeable lens designs on short-term choroidal response, axial length, and retinal defocus profile

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International Journal of Ophthalmology(English edition) 2024年第2期17卷 247-256页

作者： muteb Alanazi Patrick Caroline Amane alshamrani maria Liu Optometry Department College of Applied Medical SciencesKing Saud UniversityRiyadh 14813Saudi Arabia College of Optometry Pacific UniversityForest GroveOregon 97116-1756United States Optometry School University of California at BerkeleyCalifornia 94720United States

AIm:To investigate the impact of multifocal gas permeable contact lens(mFGPCL)in various add power and distance/near area allocation on short-term changes of choroidal thickness(ChT),axial length(AL),and retinal defocus profile in young ***:Seventeen young adults(2 males and 15 females;age 23.17±4.48y)were randomly assigned to wear two designs binocularly with a one-week washout period in *** of four mFGPCL designs were *** designs were distance-center that varied in two add power(+1.50 and 3.00 D)and/or two distance zone(DZ)diameters(1.50 and 3.00 mm;design A:DZ 1.5/add 3.0,B:DZ 1.5/add 1.5,C:DZ 3.0/add 3.0,D:DZ 3.0/add 1.5).ChT,AL,and peripheral refraction data were collected on each subject at baseline,on days 1 and 7 of mFGPCL daily *** was assessed in four quadrants using a spectraldomain optical coherence ***:AL was shortened by-26±44μm with lens C,-18±27μm with lens D,-13±29μm with lens A,and-8±30μm with lens B(all Pm,B:+3±7μm,C:+8±7μm,and D:+8±7μm).Temporal and superior choroid exhibited more choroidal thickening associated with *** designs induced significant relative peripheral myopia(RPm)beyond the central 20o across the horizontal meridian in both nasal and temporal fields(PmFGPCLs show a significant influence on ChT and AL,which are associated with significant increase in RPm after short-term *** reliability and feasibility of quantifying short-term changes in ChT support its use as a promising marker for the long-term efficacy of myopia-controlling treatments.

关键词： choroid gas-permeable contact lens retinal defocus axial length myopia

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modeling and stability analysis of HIV/HTLV-I co-infection

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International Journal of Biomathematics 2021年第5期14卷 97-143页

作者： A.m.Elaiw N.H.alshamrani Department of mathematics Faculty of Science King Abdulaziz University P.O.Box 80203Jeddah 21589Saudi Arabia Department of mathematics Faculty of Science Al-Azhar UniversityAssiut BranchAssiutEgypt Department of mathematics Faculty of Science University of Jeddah P.O.Box 80327Jeddah 21589Saudi Arabia

Human immunodeficiency virus(HIV)and human T-Iymphotropic virus type I(HTLV-I)are two retroviruses that infect the susceptible CD4^(+)T *** is known that HIV and HTLV-I have in common a way of transmission through direct contact with certain body fluids related to infected ***,it is not surprising that a mono-infected person with one of these viruses can be co-infected with the other *** the literature,a great number of mathematical models has been presented to describe the within-host dynamics of HIV or HTLV-I ***,the within-host dynamics of HIV/HTLV-I co-infection has not been *** this paper,we develop a new within-host HIV/HTLV-I co-infection *** model includes the impact of Cytotoxic T lymphocytes(CTLs)immune response,which is important to control the progression of viral *** model describes the interaction between susceptible CD4^(+)T cells,silent HIV-infected cells,active HIV-infected cells,silent HTLV-infected cells,Tax-expressing HTLV-infected cells,free HIV particles,HIV-specific CTLs and HTLV-specific *** first show the nonnegativity and boundedness of the model’s solutions and then we calculate all possible *** derive the threshold parameters which govern the existence and stability of all equilibria of the *** prove the global asymptotic stability of all equilibria by utilizing Lyapunov function and LaSalle’s invariance *** have presented numerical simulations to illustrate the effectiveness of our main *** addition,we discuss the effect of HTLV-I infection on the HIV-infected patients and vice versa.

关键词： HIV/HTLV-I co-infection global stability CTL-mediated immune response Lyapunov function

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Impact of adaptive immune response and cellular infection on delayed virus dynamics with multi-stages of infected cells

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International Journal of Biomathematics 2020年第1期13卷 79-140页

作者： A.m.Elaiw N.H.alshamrani Department of mathematics Faculty of Science King Abdulaziz UniversityP.O.Box 80203 Jeddah 21589Saudi Arabia Department of mathematics Faculty of Science University of JeddahP.O.Box 80327 Jeddah 21589Saudi Arabia

In this investigation,we propose and analyze a virus dynamics model with multi-stages of infected *** model incorporates the effect of both humoral and cell-mediated immune *** consider two modes of transmissions,virus-to-cell and *** intracellular discrete-time delays have been integrated into the *** incidence rate of infection as well as the generation and removal rates of all compartments are described by general nonlinear *** derive five threshold parameters which determine the existence of the equilibria of the model under consideration.A set of conditions on the general functions has been established which is sufficient to investigate the global stability of the five equilibria of the *** global asymptotic stability of all equilibria is proven by utilizing Lyapunov function and LaSalle’s invariance *** theoretical results are illustrated by numerical simulations of the model with specific forms of the general functions.

关键词： Viral and cellular infections global stability adaptive immune response Lyapunov function multi-staged infected cells.

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Global stability of a delayed virus dynamics model with multi-staged infected progression and humoral immunity

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International Journal of Biomathematics 2016年第4期9卷 193-218页

作者： A. m. Etaiw N. H. alshamrani Department of mathematics Faculty of Science King Abdulaziz University P. O. Box 80203 Jeddah 21589 Saudi Arabia

In this paper, we propose a nonlinear virus dynamics model that describes the interac- tions of the virus, uninfected target cells, multiple stages of infected cells and B cells and includes multiple discrete delays. We assume that the incidence rate of infection and removal rate of infected cells are given by general nonlinear functions. The model can be seen as a generalization of several humoral immunity viral infection model presented in the literature. We derive two threshold parameters and establish a set of conditions on the general functions which are sufficient to establish the existence and global stability of the three equilibria of the model. We study the globa! asymptotic stability of the equilibria by using Lyapunov method. We perform some numerical simulations for the model with specific forms of the general functions and show that the numerical results are consistent with the theoretical results.

关键词： Virus dynamics intracellular delay global stability humoral immune res-ponse Lyapunov functional.

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Liver Ailment Prediction Using Random Forest model

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Computers, materials & Continua 2023年第1期74卷 1049-1067页

作者： Fazal muhammad Bilal Khan Rashid Naseem Abdullah A Asiri Hassan A alshamrani Khalaf A alshamrani Samar m Alqhtani muhammad Irfan Khlood m mehdar Hanan Talal Halawani Department of Electrical Engineering University of Engineering and TechnologyMardan23200Pakistan City University of Science and Information Technology PeshawarPakistan Department of IT and Computer Science Pak-Austria Fachhochschule Institute of Applied Sciences and TechnologyHaripurPakistan Radiological Sciences Department College of Applied Medical SciencesNajran UniversityNajranSaudi Arabia Department of Information Systems College of Computer Science and Information SystemsNajran UniversityNajran61441Saudi Arabia Electrical Engineering Department College of EngineeringNajran University Saudi ArabiaNajran61441Saudi Arabia Anatomy Department Medicine CollegeNajran UniversityNajranSaudi Arabia Computer Science Department College of Computer Science and Information SystemsNajran UniversityNajranSaudi Arabia

Today,liver disease,or any deterioration in one’s ability to survive,is extremely common all around the *** research has indicated that liver disease is more frequent in younger people than in older *** the liver’s capability begins to deteriorate,life can be shortened to one or two days,and early prediction of such diseases is *** several machine learning(mL)approaches,researchers analyzed a variety of models for predicting liver disorders in their early *** a result,this research looks at using the Random Forest(RF)classifier to diagnose the liver disease early *** dataset was picked from the University of California,Irvine ***’s accomplishments are contrasted to those of multi-Layer Perceptron(mLP),Average One Dependency Estimator(A1DE),Support Vector machine(SVm),Credal Decision Tree(CDT),Composite Hypercube on Iterated Random Projection(CHIRP),K-nearest neighbor(KNN),Naïve Bayes(NB),J48-Decision Tree(J48),and Forest by Penalizing Attributes(Forest-PA).Some of the assessment measures used to evaluate each classifier include Root Relative Squared Error(RRSE),Root mean Squared Error(RmSE),accuracy,recall,precision,specificity,matthew’s Correlation Coefficient(mCC),F-measure,and *** has an RRSE performance of 87.6766 and an RmSE performance of 0.4328,however,its percentage accuracy is *** widely acknowledged result of this work can be used as a starting point for subsequent *** a result,every claim that a new model,framework,or method enhances forecastingmay be benchmarked and demonstrated.

关键词： Liver ailment random forest machine learning

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Human Emotions Classification Using EEG via Audiovisual Stimuli and AI

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Computers, materials & Continua 2022年第12期73卷 5075-5089页

作者： Abdullah A Asiri Akhtar Badshah Fazal muhammad Hassan A alshamrani Khalil Ullah Khalaf A alshamrani Samar Alqhtani muhammad Irfan Hanan Talal Halawani Khlood m mehdar Radiological Sciences Department College of Applied Medical SciencesNajran UniversityNajranSaudi Arabia Faculty of Computer Science and Engineering GIK Institute of Engineering Sciences and TechnologyTopi23460Pakistan Department of Electrical Engineering University of Engineering and TechnologyMardan23200Pakistan Department of Software Engineering University of MalakandDir LowerPakistan Department of Computer Science and Information Systems College of EngineeringNajran University Saudi ArabiaNajran61441Saudi Arabia Electrical Engineering Department College of EngineeringNajran University Saudi ArabiaNajran61441Saudi Arabia Computer Science Department College of Computer Science and Information SystemsNajran UniversityNajranSaudi Arabia Anatomy Department Medicine CollegeNajran UniversityNajranSaudi Arabia

Electroencephalogram(EEG)is a medical imaging technology that can measure the electrical activity of the scalp produced by the brain,measured and recorded chronologically the surface of the scalp from the *** recorded signals from the brain are rich with useful *** inference of this useful information is a challenging *** paper aims to process the EEG signals for the recognition of human emotions specifically happiness,anger,fear,sadness,and surprise in response to audiovisual *** EEG signals are recorded by placing neurosky mindwave headset on the subject’s scalp,in response to audiovisual stimuli for the mentioned *** a bandpass filter with a bandwidth of 1-100 Hz,recorded raw EEG signals are *** preprocessed signals then further analyzed and twelve selected features in different domains are *** Random forest(RF)and multilayer perceptron(mLP)algorithms are then used for the classification of the emotions through extracted *** proposed audiovisual stimuli based EEG emotion classification system shows an average classification accuracy of 80%and 88%usingmLP and RF classifiers respectively on hybrid features for experimental signals of different *** proposed model outperforms in terms of cost and accuracy.

关键词： Electroencephalography emotion classification signal processing multilayer perceptron random forest

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Cancellable multi-Biometric Feature Veins Template Generation Based on SHA-3 Hashing

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Computers, materials & Continua 2023年第1期74卷 733-749页

作者： Salwa m.Serag Eldin Ahmed Sedik Sultan S.alshamrani Ahmed m.Ayoup Department of Computer Engineering College of Computers and Information TechnologyTaif UniversityP.O.Box 11099Taif21944Saudi Arabia Department of Robotics and Intelligent machine Faculty of Artificial IntelligentKafrelsheikh UniversityEgypt Department of Information Technology College of Computer and Information TechnologyTaif UniversityP.O.Box 11099Taif21944Saudi Arabia Department of Electrical Communications Engineering Faculty of EngineeringMinia UniversityMinia61111Egypt

In this paper,a novel cancellable biometrics technique calledmulti-Biometric-Feature-Hashing(mBFH)is *** mBFH strategy is utilized to actualize a single direction(non-invertibility)biometric *** is a typical model security conspire that is distinguished in the utilization of this protection insurance framework in numerous sorts of biometric feature strategies(retina,palm print,Hand Dorsum,fingerprint).A more robust and accurate multilingual biological structure in expressing human loneliness requires a different format to record clients with inseparable comparisons from individual biographical *** may raise worries about their utilization and security when these spread out designs are subverted as everybody is acknowledged for another biometric *** proposed structure comprises of four sections:input multi-biometric acquisition,feature extraction,multi-Exposure Fusion(mEF)and secure hashing calculation(SHA-3).multimodal biometrics systems that are more powerful and precise in human-unmistakable evidence require various configurations to store a comparative customer that can be contrasted with biometric wellsprings of *** top words,biometrics graphs can’t be denied and change to another request for positive Identifications(IDs)while *** biometrics is may be the special procedure used to recognize this issue.

关键词： Feature extraction multi-biometrics SHA-3 mEF

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