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Occult HCV Infection (OCI) Diagnosis in Cirrhotic and Non-cirrhotic Naive Patients by Intra-PBMC Nested Viral RNA PCR

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Journal of Clinical and Translational Hepatology 2017年第4期5卷 319-326页

作者： Mohamed Darwish ahmed Abd Alla saleh ahmed elibiary George Y.Wu Mostafa Kamel El-Awady Tropical Medicine Department Faculty of MedicineAl-Azhar UniversityCairoEgypt Department of Medicine Division of Gastroenterology-HepatologyUniversity of Connecticut Health CenterFarmingtonCTUSA Department of Microbial Biotechnology National Research CenterCairoEgypt

Background and Aims:Occult HCV infections(OCIs)include IgG antibody seronegative cryptogenic(COCIs),as well as seropositive secondary na(i)ve(SNOCIs)and experienced(SEOCIs)*** used peripheral-blood-mononuclear-cell(PBMC)-PCR to evaluate COCIs and SNOCIs prevalence,serum HCV spontaneous disappearance(SCSD)in na(i)ve cirrhotics and non-cirrhotics,intra-PBMC HCV-RNA strands in relation to cirrhosis density in na(i)ve non-viremia cases,and HCV-RNA seroconversion after 1 year of solitary naive intra-PBMC ***:The anti-HCV IgG antibodypositive na(i)ve-patients(n=785)were classified into viremic(n=673)and non-viremic[n=112,including non-cirrhotics(n=55)and cirrhotics(n=57)],and 62 controls without evidence of *** and post-HCV non-viremia cases(n=62+112=174)were submitted to hepatic FibroscanElastography *** subjects(n=847)were screened for intra-PBMC HCV-RNA sense and antisense strands by ***:Na(i)ve-OCI cases(4.84%)that were diagnosed by PBMC-PCR significantly raised the total numbers of HCV-infection to 714(p=0.01).The percent positivity of SNOCIs(34.82%)was significantly higher than for asymptomatic-COCIs(3.125%,p=0.0001).Comparing PBMC-PCR with single-step-reverse-transcription(SRT)-PCR for identification of SCSD in na(i)ve IgG antibody-positive nonviremia patients(n=112)revealed a decline in SCSD prevalence by PBMC-PCR(from 14.27%to 9.3%),regardless of presence of hepatic cirrhosis(p=0.03).SCSD was found to be higher by PBMC-PCR in non-cirrhotics compared to cirrhotics(p=0.0001),with an insignificant difference when using SRT-PCR(p=0.45).Intra-PBMC HCV-RNA infection was significantly more frequent in cirrhotics compared to both non-cirrhotics and controls(p<0.0005).An increased hepatic fibrosis density was recognized in intra-PBMC HCV-RNA infection with sense(p=0.0001)or antisense strand(p=0.003).HCV-RNA seroconversion was associated with intra-PBMC infection when both sense and antisense strands were dete

关键词： OCIs PBMCs Na(i)ve Cirrhosis

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HCV Therapy Follow-up Fractionation (CTF2) by Intra-PBMC Nested RNA PCR Recognizes Early Virologic Response and Relapse

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Journal of Clinical and Translational Hepatology 2018年第2期6卷 147-154页

作者： Mohamed Darwish ahmed Abd Alla saleh ahmed elibiary Ramy Hassan Elshaboury George Y.Wu Reham M.Dawood Mostafa Kamel El Awady Tropical Medicine Department Faculty of MedicineAl-Azhar UniversityCairoEgypt Department of Pharmacy Massachusetts General HospitalBostonMAUSA Department of Medicine Division of Gastroenterology-HepatologyUniversity of Connecticut Health CenterHartfordCTUSA Department of Microbial Biotechnology National Research CenterCairoEgypt

Background and Aims:Sustained virologic response is evaluated by single-step reverse transcription(SRT)PCR as-say,which assesses hepatitis C virus(HCV)clearance from plasma but not from tissues such as peripheral blood mono-nuclear cells(PBMCs).Persistence of HCV RNA in PBMCs beyond end of treatment(EOT)is associated with *** goal was to measure intra-PBMC HCV RNA levels during oral antiviral therapy according to the HCV therapy follow-up fractionation(CTF2)***:Compen-sated chronic HCV patients(n=278 SRT-PCR positive)were scheduled to receive oral antiviral *** were followed-up by SRT and intra-PBMCs HCV RNA PCR at the end of the 2nd,6th,10th,14th,18th and 24th weeks to evaluate virus clearance from plasma and PBMCs,*** CTF2 protocol evaluated SRT and PBMC PCR status at each follow-up point for determining therapy continuation or inter-ruption to address cost ***:All patients tested negative by SRT PCR after therapy for 2 ***-cation of the CTF2 protocol revealed:a)increasing HCV clear-ance rate from 75.9%at the end of 10th week to 90.3%at the end of 24th week(p<0.00001);b)faster clearance of HCV from plasma compared to PBMCs at each point of follow-up until the 18th week(p<0.05);c)higher viral elimination rates diagnosed by PBMC HCV RNA PCR(?)compared to PBMC HCV RNA PCR(+)from the 6th to 24th week of treatment(p<0.0001);d)higher over-time increase curve of combined plasma and PBMC HCV RNA determined negativity compared to the decline in positivity curves by PBMC PCR at the 6th-18th week compared to the 24th week(p<0.01)—these results validated treatment continuation;and e)solitary evaluation of EOT sustained HCV infection and relapses by PBMC HCV RNA(p<0.001).Conclusions:Early elimination of serum and tissue(PBMC)HCV infection by oral antiviral therapy can be achieved and evaluated during a cost-effective CTF2 pro-tocol application.

关键词： CTF2 protocol HCV PBMCs PCR Cost-effectiveness

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Blockchain for secure and decentralized artificial intelligence in cybersecurity: A comprehensive review

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Blockchain(Research and Applications) 2024年第3期5卷 184-208页

作者： ahmed M.Shamsan saleh Department of Information Technology University of TabukTabuk47512Saudi Arabia

As the use of artificial intelligence (AI) grows within the field of cybersecurity, so does the demand for secure and decentralized AI systems to protect against potential cyber threats. Blockchain technology has emerged as the ideal approach for increasing both the security and privacy of AI systems since it provides decentralized and immutable data storage. This systematic literature review focuses on the integration of blockchain technology with decentralized AI within cybersecurity. It provides a comprehensive taxonomy of blockchain technology and decentralized AI for cybersecurity, serving as the starting point for the study. This paper begins with an overview of blockchain technology and its possible uses in cybersecurity and also analyzes its challenges and opportunities. Decentralized AI is also covered in the study, along with its potential advantages and difficulties in decentralized AI cybersecurity. Building on that foundation, this study provides convincing findings highlighting the beneficial relationships between blockchain technology and decentralized AI for cybersecurity, contributing to a nuanced comprehension of their integration. Moreover, it offers real-world uses of blockchain-enabled, decentralized AI to discuss its usefulness in tackling cybersecurity concerns. Finally, this paper discusses future research directions for blockchain-enabled decentralized AI in cybersecurity, including potential applications and implications of this technology in the field, emphasizing the potential of blockchain-enabled decentralized AI in cybersecurity to enhance security, privacy, and trust in AI systems.

关键词： Blockchain Artificial intelligence Decentralized Cybersecurity

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The interplay mechanism between IDH mutation, MGMT-promoter methylation, and PRMT5 activity in the progression of grade 4 astrocytoma: unraveling the complex triad theory

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Oncology Research 2024年第6期32卷 1037-1045页

作者： MAHER KURDI ALAA ALKHOTANI ABDULRAHMAN SABBAGH EYAD FAIZO ahmed I.LARY ahmed K.BAMAGA MAJID ALMANSOURI BADR HAFIZ THAMER ALSHARIF saleh BAEESA Department of Pathology Faculty of MedicineKing Abdulaziz UniversityRabighSaudi Arabia Department of Pathology College of MedicineUmm Al-Qura UniversityMakkahSaudi Arabia Department of Surgery Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Department of Surgery Faculty of MedicineUniversity of TabukTabukSaudi Arabia Section of Neurosurgery Department of SurgeryKing Abdulaziz Medical CityJeddahSaudi Arabia Department of Pediatrics Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Department of Clinical Biochemistry Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Department of Neurosciences King Faisal Specialist Hospital and Research CenterJeddahSaudi Arabia Department of Surgery King Abdulaziz Specialist HospitalTaifSaudi Arabia

Background:The dysregulation of Isocitrate dehydrogenase(IDH)and the subsequent production of 2-Hydroxyglutrate(2HG)may alter the expression of epigenetic proteins in Grade 4 *** interplay mechanism between IDH,O-6-methylguanine-DNA methyltransferase(MGMT)-promoter methylation,and protein methyltransferase proteins-5(PRMT5)activity,with tumor progression has never been ***:A retrospective cohort of 34 patients with G4 astrocytoma is classified into IDH-mutant and IDH-wildtype *** groups were tested for MGMT-promoter methylation and PRMT5 through methylation-specific and gene expression PCR ***-cohort statistical significance was ***:Both IDH-mutant WHO grade 4 astrocytomas(n=22,64.7%)and IDH-wildtype glioblastomas(n=12,35.3%)had upregulated PRMT5 gene expression except in one *** of the 22 IDH-mutant tumors,10(45.5%)tumors showed MGMT-promoter methylation and 12(54.5%)tumors had unmethylated *** IDH-wildtype tumors had unmethylated *** was a statistically significant relationship between MGMT-promoter methylation and IDH in G4 astrocytoma(p-value=0.006).Statistically significant differences in progression-free survival(PFS)were also observed among all G4 astrocytomas that expressed PRMT5 and received either temozolomide(TMZ)or TMZ plus other chemotherapies,regardless of their IDH or MGMT-methylation status(p-value=0.0014).Specifically,IDH-mutant tumors that had upregulated PRMT5 activity and MGMT-promoter methylation,who received only TMZ,have exhibited longer ***:The relationship between PRMT5,MGMT-promoter,and IDH is not ***,accumulation of D2-hydroxyglutarate(2-HG),which partially activates 2-OG-dependent deoxygenase,may not affect their *** IDH-wildtype glioblastomas,the 2HG-2OG pathway is typically inactive,leading to PRMT5 *** alone,compared to TMZ-plus,can increase PFS in upregulated PRMT5 ***,using a PRMT5 inhibitor in G4

关键词： Grade 4 astrocytoma Glioblastoma Isocitrate dehydrogenase(IDH) O-6-methylguanine-DNA methyltransferase(MGMT) Protein methyltransferase proteins-5(PRMT5) Progression-free survival(PFS)

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Kaoline-based catalyst for a high stability desulfurization of sour heavy naphtha in a three-phase oscillatory baffled reactor

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Particuology 2024年第1期84卷 249-260页

作者： Hassan M.Hmood Saba A.Gheni Safaa M.R.ahmed Mudheher M.Ali Hamid Yonis saleh Mohammed H.Mohammed Awad E.Mohammed Marwan A.Mahomood Hiba R.Mohammed ahmed A.Hassan Adam Harvey North Refineries Company Ministry of OilIraq Chemical Engineering Department Tikrit UniversityIraq Ministry of Oil Iraq AL-Hawija Technical Institute Northern Technical UniversityMoselIraq School of Engineering Newcastle UniversityUK

To meet the rapidly increasing demand for energy and the dramatic depletion of conventional crude oil,it is imperative to utilize sour *** no coke being produced,oxidative desulfurization(ODS)of naphtha lowers its sulfur content and average molecular *** this article,we outline a method for heavy naphtha non-extractive ODS using a very stable *** technique involves the use of a solid catalyst with oxygen gas as the *** necessitated relatively high mixing intensities;hence a three-phase Oscillatory Baffled Reactor(OBR)was *** catalyst was based on the zeolite ZSM-5,prepared from natural kaolin by a series of delamination and activation steps and impregnated with Fe.A TiO2 nanolayer was applied,using the sol-gel method,to prevent rapid *** reactor performance was evaluated to minimize the sulfur content in the naphtha *** to the protective coating,the sulfur conversion stabilized at 90%.The results of this work establish the use of natural clay-based catalysts in a continuous,three-phase ODS,particularly with regard to proving long-term *** also showed that modest ODS can be achieved using an environmentally friendly oxidant,at mild operating conditions,whilst maintaining stability.

关键词： Natural kaoline Desulfurization Three-phase Oscillatory baffled reactor

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加速康复外科对初次全膝关节置换术后血液管理的影响：倾向评分匹配分析

加速康复外科对初次全膝关节置换术后血液管理的影响：倾向评分匹...

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作者： Mogalli Abdullah saleh Hussein ahmed 吉林大学

学位级别：硕士

背景:全膝关节置换术(TKA)是缓解慢性膝关节炎患者关节疼痛和增强关节功能的重要手段。TKA的并发症常常与大量失血相关,并直接导致术后血红蛋白水平下降,从而高达69%的患者需要异体输血来应对术后贫血。这已被证明是增加不良结果风险的... 详细信息

背景:全膝关节置换术(TKA)是缓解慢性膝关节炎患者关节疼痛和增强关节功能的重要手段。TKA的并发症常常与大量失血相关,并直接导致术后血红蛋白水平下降,从而高达69%的患者需要异体输血来应对术后贫血。这已被证明是增加不良结果风险的独立因素,包括延长住院时间和增加术后感染的易感性。由丹麦学者Henrik Kehlet于1997年首次提出的加速康复外科(ERAS)已成为现代外科实践中的一个关键方法。ERAS最初在腹部手术中实施,逐渐扩展到包括骨科和泌尿科在内的各个医学专业。然而,尽管ERAS方案的采用日益增加,系统评估关节置换术后的血液指标仍然有限。这种多方面的策略在减轻手术压力、加速术后恢复、减少住院时间和提高整体功能结果方面发挥着重要作用,同时保持低并发症率。之前的研究已证明ERAS在减少失血和优化全髋关节置换术结果方面的有效性。问题是这些发现是否也适用于TKA。THA和TKA之间的关键区别,包括患者人口统计学、手术时间和软组织损伤程度,强调了优化每项手术中患者护理的需要。TKA患者通常比THA患者平均年龄更大,皮肤对皮肤时间更长,并且由于股骨和胫骨截骨术,软组织损伤增加。ERAS方案有效控制围手术期失血量。ERAS组术后CRP和ESR水平较低,表明炎症和凝血活动减少,这可能归因于ERAS计划提供的全面护理,其中包括人性化和以患者为中心的干预措施,以减轻焦虑。ERAS手术已显示出相当大的好处,例如通过利用术前优化、术中程序和术后管理等循证策略,最大限度地减少围手术期失血、降低并发症和改善营养状况。WOMAC、OKS和SF-12的理想结果表明ERAS方案有利于改善术后短期内的短期恢复和生活质量结果。目的:评估在加速康复外科(ERAS)下进行单侧全膝关节置换术(TKA)的患者的血液指标管理效果。材料与方法:回顾性研究了2019年1月至2023年10月在我们医院接受单侧TKA的患者。通过使用0.2校准器进行倾向评分匹配后,这些病例被分为两组:非ERAS组(n=51)接受标准护理,ERAS组(n=51)接受全面ERAS管理。术后比较了主要血液参数/血液指标,同时在第3个月随访时也评估了临床结果。结果:ERAS组术后Hb明显高于非ERAS组(P<0.05)。非ERAS组术前ESR明显高,而ERAS组CRP明显低于非ERAS组(P<0.05)。ERAS组的LOS和Alb明显少于非ERAS组(P<0.05)。WOMAC和OKS得分在第1个月和第3个月均在ERAS组。结论:ERAS方案在术后第一天到第三个月的术后期间提供了良好且现实的临床和功能结果,对接受单侧初次TKA的患者满意度很高。此外,ERAS方案已被证明可以有效减少围手术期失血、最大限度地减少术后并发症并维持这些患者的最佳营养水平。

关键词：全膝关节置换术快速康复血液管理住院时间并发症随访

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Takotsubo Cardiomyopathy Triggered by Acute Pancreatitis: A Case Report

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Case Reports in Clinical Medicine 2024年第4期13卷 87-94页

作者： Ketevan Kapanadze Elene Shengelia Keti Solomnishvili ahmed saleh Abdelkader Maha Kassem Internal Medicine and Intensive Therapy Department Tbilisi Central Hospital Tbilisi Georgia Internal Medicine Residency Program Tbilisi State Medical University Tbilisi Georgia School of Medicine New Vision University Tbilisi Georgia

Takotsubo cardiomyopathy is a heart condition that is widely known to be caused by stress. It presents with symptoms that are similar to a myocardial infarction even though the coronary arteries are clear. This case report details the clinical characteristics, diagnostic assessment, and management plan of a 55-year-old male patient with a past medical history of alcoholism who arrived at the emergency department with the typical symptoms of acute pancreatitis. The case demonstrates the progression of Takotsubo cardiomyopathy, which was triggered by acute pancreatitis in the context of alcoholism, and underlines the significance of early detection and management to enhance the patient’s outcomes.

关键词： Takotsubo Cardiomyopathy Acute Pancreatitis Alcoholism Epigastric Pain

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Optimizing Grey Wolf Optimization: A Novel Agents’ Positions Updating Technique for Enhanced Efficiency and Performance

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Open Journal of Optimization 2024年第1期13卷 21-30页

作者： Mahmoud Khatab Mohamed El-Gamel ahmed I. saleh Asmaa H. Rabie Atallah El-Shenawy Department of Mathematics and Engineering Physics Faculty of Engineering Mansoura University Mansoura Egypt Department of Computers and Control Faculty of Engineering Mansoura University Mansoura Egypt Department of Mathematics Faculty of Science New Mansoura University Mansoura Egypt

Grey Wolf Optimization (GWO) is a nature-inspired metaheuristic algorithm that has gained popularity for solving optimization problems. In GWO, the success of the algorithm heavily relies on the efficient updating of the agents’ positions relative to the leader wolves. In this paper, we provide a brief overview of the Grey Wolf Optimization technique and its significance in solving complex optimization problems. Building upon the foundation of GWO, we introduce a novel technique for updating agents’ positions, which aims to enhance the algorithm’s effectiveness and efficiency. To evaluate the performance of our proposed approach, we conduct comprehensive experiments and compare the results with the original Grey Wolf Optimization technique. Our comparative analysis demonstrates that the proposed technique achieves superior optimization outcomes. These findings underscore the potential of our approach in addressing optimization challenges effectively and efficiently, making it a valuable contribution to the field of optimization algorithms.

关键词： Grey Wolf Optimization (GWO) Metaheuristic Algorithm Optimization Problems Agents’ Positions Leader Wolves Optimal Fitness Values Optimization Challenges

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Practical guide:Glucagon-like peptide-1 and dual glucosedependent insulinotropic polypeptide and glucagon-like peptide-1 receptor agonists in diabetes mellitus

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World Journal of Diabetes 2024年第3期15卷 331-347页

作者： saleh Fahad Alqifari Omar Alkomi Abdullah Esmail Khadijeh Alkhawami Shahd Yousri Mohamad Ayham Muqresh Nawwarah Alharbi Abdullah A Khojah ahmed Aljabri Abdulrahman Allahham Kousalya Prabahar Hanan Alshareef Mohammed Aldhaeefi Tariq Alrasheed Ali Alrabiah Laila A AlBishi Department of Pharmacy Practice Faculty of PharmacyUniversity of TabukTabuk 71491Saudi Arabia Department of Clinical Sciences Sulaiman Alrajhi University-College of MedicineAl Bukayriyah 52726Saudi Arabia Department of Family Medicine Dr.Soliman Fakeeh Hospital DSFHJeddah 21461Saudi Arabia Department of Pharmacy Practice Faculty of PharmacyKing Abdulaziz UniversityJeddah 22252Saudi Arabia Clinical and Administrative Pharmacy Sciences College of PharmacyHoward UniversityWashingtonDC 20059United States Department of Internal Medicine Faculty of MedicineUniversity of TabukTabuk 71491Saudi Arabia Department of Pharmacy Practice Raabe College of PharmacyOhio Northern UniversityOhioOH 45810United States Department of Pediatric Faculty of MedicineUniversity of TabukTabuk 71491Saudi Arabia

Practical guide:Glucagon-like peptide-1 and dual glucosedependent insulinotropic polypeptide and glucagon-like peptide-1 receptor agonists in diabetes mellitus common second-line choice after metformin for treating *** considerations can make selecting and switching between different GLP-1 RAs *** study aims to provide a comprehensive guide for the usage of GLP-1 RAs and dual GIP and GLP-1 RAs for the management of T2DM.

关键词： Glucagon-like peptide-1 receptor agonist Diabetes mellitus Metabolic syndrome Dual glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1 receptor agonist Clinical practice Endocrinology

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Evaluation of the Strategy and Efficacy of Treatment of Chronic Viral Hepatitis C with the Sofosbuvir/Daclatasvir Combination in a Resource-Limited Country

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Open Journal of Gastroenterology 2024年第7期14卷 233-240页

作者： Mayanna Habkreo Ali Mahamat Moussa Adama ahmed Ngare Abdraman Kossou Tahir Mahamat saleh Maire Dehainsala Mahamat Ali Hachim Mahamat Ali Bolti Oumaima Djarma Joseph Madtoingue Service de Gastroentrologie/Mdecine Interne du Centre Hospitalier Universitaire la Rfrence Nationale NDjamena Chad Service de Gastroentrologie du Centre Hospitalier Universitaire la Renaissance NDjamena Chad Service de Maladies Infectieuses du Centre Hospitalier Universitaire la Renaissance NDjamena Chad Service de Maladies Infectieuses du Centre Hospitalier Universitaire la Rfrence Nationale NDjamena Chad

Introduction: The treatment of viral hepatitis C (HCV), a major public health problem, has evolved considerably since the introduction of direct-acting anti-virals (DAAs). The aim of this study was to evaluate the strategy for initiating treatment with Sofosbuvir/Daclatasvir, and also to assess its efficacy. Patients and Methods: Included were patients aged at least 15 years, with detectable hepatitis C viremia and treated with a pan-genotypic Sofosbuvir/Daclatasvir regimen at the Centre “Hospitalier Universitaire la Référence Nationale de N’Djamena” between October 2019 and October 2023. The APRI score was used to assess fibrosis. Cure was defined by an undetectable viral load 12 weeks after the end of treatment. Results: A total of 835 patients were included (sex ratio 1.55, mean age 50.5 ± 13.73, extremes 20 and 84 years). The 60 - 69 age group was the most represented. The mean APRI score was 0.42 ± 0.29, with extremes of 0.019 and 1.84. The mean viral load was 2,316,193.086 IU/mL. All subjects had good renal function. Cytolysis was absent in 70% of cases. Without genotyping, a pan-genotypic regimen was used in all patients: Sofosbuvir (400 mg)/Daclatasvir (60 mg). The cure rate was 99.4%, with good tolerability. However, four cases of failure were recorded out of the 668 patients who underwent follow-up HCV-PCR testing after 12 weeks of treatment. Conclusion: The Sofosbuvir/Daclatasvir regimen is highly effective, with an excellent safety profile. However, the still high cost of these molecules limits their accessibility to a large proportion of patients.

关键词： Efficacy Tolerance Sofosbuvir/Daclatasvir HCV Chad

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