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Screening agars for MRSA:evaluation of a stepwise diagnostic approach with two different selective agars for the screening for methicillin-resistant Staphylococcus aureus(MRSA)

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Military Medical Research 2015年第4期3卷 213-219页

作者： Volker Micheel Benedikt Hogan Thomas Koller Philipp Warnke sabine crusius Rebecca Hinz Ralf Matthias Hagen Norbert Georg Schwarz Hagen Frickmann Department of Tropical Medicine at the Bernhard Nocht Institute German Armed Forces Hospital of Hamburg Department of Infectious Disease Epidemiology Bernhard Nocht Institute for Tropical Medicine Hamburg Institute for Microbiology Virology and Hygiene University Medicine Rostock

Background: Colonization with methicillin-resistant Staphylococcus aureus(MRSA) poses a hygiene risk that does not spare field hospitals or military medical field camps during military deployments. Diagnostic options for unambiguously identifying MRSA isolates are usually scarce in military environments. In this study, we assessed the stepwise application of two different selective agars for the specific identification of MRSA in screening ***: Nasal swabs from 1,541 volunteers were subjected to thioglycollate broth enrichment and subsequently screened on CHROMagar MRSA selective agar for the identification of MRSA. The MRSA identity of suspiciouslooking colonies was confirmed afterwards or excluded by another selective agar, chrom ID MRSA. All isolates from the selective agars with MRSA-specific colony morphology were identified by biochemical methods and mass ***: The initial CHROMagar MRSA screening identified suspicious colonies in 36 out of 1541 samples. A total of 25 of these 36 isolates showed MRSA-like growth on chrom ID agar. Out of these 25 isolates, 24 were confirmed as MRSA, while one isolate was identified as Staphylococcus kloosii. From the 11 strains that did not show suspicious growth on chrom ID agar, 3 were methicillin-sensitive Staphylococcus aureus(MSSA, with one instance of cocolonization with Corynebacterium spp.), 2 were confirmed as MRSA(with 1 instance of co-colonization with MSSA), 2 were lost during passaging and could not be re-cultured, one could not be identified by the applied approaches, and the remaining 3 strains were identified as Staphylococcus saprophyticus, Staphylococcus hominis(co-colonized with Macrococcus caseolyticus) and Staphylococcus cohnii, ***: The application of the selective agar CHROMagar MRSA alone proved to be too non-specific to allow for a reliable diagnosis of the presence of MRSA. The combined use of two selective agars in a stepwise approach reduced this non-s

关键词： Methicillin-resistant Staphylococcus aureus Selective agar Chromogenic agar Screening Hygiene Stepwise diagnostics CHROMagar Chrom ID agar

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Data-driven analysis of chemicals,proteins and pathways associated with peanut allergy:from molecular networking to biological interpretation

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Food Science and Human Wellness 2024年第3期13卷 1322-1335页

作者： Emmanuel Kemmler Julian Braun Florent Fauchère sabine Dölle-Bierke Kirsten Beyer Robert Preissner Margitta Worm Priyanka Banerjee Institute for Physiology Charité-University Medicine BerlinBerlin 10115Germany Member of the KFO339 FOOD@Berlin 10115Germany Si-M/“Der Simulierte Mensch”a science framework of Technische Universität Berlin and Charité-Universitätsmedizin Berlin Berlin 10115Germany Charité-Universitätsmedizin Berlin corporate member of Freie Universität BerlinHumboldt-Universität zu Berlinand Berlin Institute of HealthBerlin 10115Germany Division of Allergy and Immunology Department of DermatologyVenerology and AllergyCharité-Universitätsmedizin Berlincorporate member of Freie Universität Berlin and Humboldt-Universität zu BerlinBerlin 10115Germany Pediatrics Campus VirchowCharité-University Medicine BerlinBerlin 10115Germany

Peanut allergy is majorly related to severe food induced allergic *** food including cow's milk,hen's eggs,soy,wheat,peanuts,tree nuts(walnuts,hazelnuts,almonds,cashews,pecans and pistachios),fish and shellfish are responsible for more than 90%of food ***,we provide promising insights using a large-scale data-driven analysis,comparing the mechanistic feature and biological relevance of different ingredients presents in peanuts,tree nuts(walnuts,almonds,cashews,pecans and pistachios)and ***,we have analysed the chemical compositions of peanuts in different processed form raw,boiled and *** the data-driven approach we are able to generate new hypotheses to explain why nuclear receptors like the peroxisome proliferator-activated receptors(PPARs)and its isoform and their interaction with dietary lipids may have significant effect on allergic *** results obtained from this study will direct future experimeantal and clinical studies to understand the role of dietary lipids and PPARisoforms to exert pro-inflammatory or anti-inflammatory functions on cells of the innate immunity and influence antigen presentation to the cells of the adaptive immunity.

关键词： Allergy informatics Knowledge-graph Data analysis Food allergy Peroxisome proliferator-activated receptors Fatty acids

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Analysis of the Tragedy of Vila Socóin Cubatão (São Paulo, Brazil) Using the Haddon Matrix: A Comprehensive Examination of Socio-Environmental Factors and Risk Mitigation Strategies

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Journal of Environmental Protection 2024年第5期15卷 620-639页

作者： Rubens Martins Santos Nilce Ortiz sabine Neusatz Guilhen Nuclear and Energy Research Institute IPEN/SP S&#227o Paulo Brazil

The tragedy of Vila Socó epitomizes the socio-environmental repercussions of rapid industrialization in Cubatão. Beginning in the 1940s with the construction of the Anchieta highway, the city experienced an influx of migrants drawn by burgeoning industries, leading to unplanned urban growth and the emergence of vulnerable communities like Vila Socó. This article examines the interconnected factors—such as demographic shifts, inadequate planning, and regulatory oversight—that culminated in the devastating fire of 1984, claiming numerous lives and highlighting systemic failures. Utilizing the Haddon Matrix, this study dissects the Vila Socó incident, emphasizing the roles of human error, infrastructure integrity, and socio-economic disparities in disaster causation. By contextualizing the tragedy within Cubatão’s industrial trajectory, it underscores the urgent need for comprehensive risk assessment and proactive mitigation strategies in rapidly developing regions globally. Beyond its immediate focus, this work offers broader insights into the dynamics of industrial disasters and their socio-economic implications. As pipelines continue to play a vital role in global energy infrastructure, the lessons drawn from Vila Socó’s tragedy resonate deeply, emphasizing the imperative of robust safety protocols and accountable governance to prevent similar catastrophes in the future.

关键词： Haddon Matrix Vila Socó Accident Risk Analysis Cubatão

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隐藏世界——上海滴水湖洲际酒店

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中国建筑装饰装修 2024年第5期 17-23+16页

作者： sabine Beck Hirsch Bedner Associates (HBA)

这座位于上海临港新城的豪华型湖滨度假酒店是独岛酒店,有着“一岛一酒店”的美称。酒店前身为滴水湖皇冠假日酒店,其建筑造型别致,宛若一朵在湖水上盛开的桃花临湖亲水,景致迷人。本次翻新设计以“隐藏世界”为概念,打造宛如一扇连接...

这座位于上海临港新城的豪华型湖滨度假酒店是独岛酒店,有着“一岛一酒店”的美称。酒店前身为滴水湖皇冠假日酒店,其建筑造型别致,宛若一朵在湖水上盛开的桃花临湖亲水,景致迷人。本次翻新设计以“隐藏世界”为概念,打造宛如一扇连接陆地和水域并通往海洋世界的门户,带给入住客人沉浸于想象和探索之境的体验。

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数字化赋能流程工业可持续发展

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流程工业 2024年第3期 16-17页

作者： sabine Mühlenkamp 《PROCESS》编辑部

在保持流程工业竞争力的同时,如何不忽视可持续发展?整个流程行业都在寻找答案,尤其是目前正处于经济危机和能源转型双重压力下,自动化在其中正发挥着关键作用。

关键词：经济危机工业可持续发展能源转型流程工业流程行业数字化竞争力双重压力

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Vanadium mineralization at Los Chihuidos sediment-hosted Cu-V deposit,Neuquén Basin,Argentina:An approach to vanadium ore forming processes

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Geoscience Frontiers 2024年第1期15卷 225-246页

作者： Ana L.Rainoldi Daniel Beaufort Marta B.Franchini Adolfo Giusiano sabine Petit Patricia Patrier M.Josefina Pons Dpto.de Geología Universidad Nacional del Sur(UNS)8000 Bahía BlancaArgentina Consejo Nacional de Investigaciones Científicas y Técnicas Centro Patagónico de Estudios MetalogenéticosArgentina Universitéde Poitiers IC2MPCNRS-UMR 7285HydrasaF-86022 Poitiers CedexFrance Universidad Nacional del Comahue Facultad de IngenieríaDepartamento de Geología y Petróleo8300 NeuquénArgentina Consultant Geologist 8300 NeuquénArgentina Universidad Nacional de Río Negro Instituto de Investigación en Paleobiología y GeologíaRío NegroArgentina

Vanadium mineralization at Los Chihuidos deposit of the Neuquén Basin is linked to the development of a redox front system related to the inflow of hydrocarbons into the red sandstone of the Huincul *** of hydrocarbons with oxidized red beds and connate water generated redox reactions where hematite was dissolved due to iron reduction resulting in the discoloration of the red *** the contact between oxidized red sandstone and reduced white sandstone,precipitation of specific mineral phases resulted in the V ore with minor amounts of *** the implementation of the redox interface,abundant V-montmorillonite and V-hematite precipitated at the more oxidizing conditions and Cu-V-corrensite-type at the more reducing conditions of the redox *** the redox front advanced with fluids constantly migrating into the reservoir,more reducing conditions were stablished,promoting chloritization and minor illitization with V-Cu incorporation and continuous upgrading of the *** ore mineralogy consists of clay minerals including V-bearing montmorillonite,Cu-V-corrensite-type,V-di-trioctahedral chlorite and Cu-tri-trioctahedral chlorite with minor V-illite-smectite mixed-layer minerals and associated secondary *** over illitization was favored due to high amounts of Fe and Mg in detrital clasts and in connate fluids and by low K availability related to low amounts of detrital *** spatial transition of V and/or Cu bearing clay minerals observed through the mineralized redox front at Los Chihuidos deposit(kaolinite→smectite→illite/smectite→corrensite-type→di-trioctahedral-chlorite→tri-trioctahedral-chlorite)and the related variation of V-Cu concentrations in bulk rock are indicative of increasing pH and decreasing Eh of resident solutions from red to white sandstones during the hypogene mineralization *** influx of Cu-rich oxidized basinal brines precipitated main copper ore with Cu-sulfides in the white sandst

关键词： Clay minerals V-chlorite Redox front Bleaching Hydrocarbons

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Evaluation of Agricultural Contamination Level by Heavy Metals and Pesticides in Sediments of Rivers and Water Bodies for Shrimp Fishing in the Lower Ouémé: Case of the Aguigadji, Ahlan and Sele Stations in Benin

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Advances in Materials Physics and Chemistry 2024年第2期14卷 25-46页

作者： Souradjou Orou Goura Fadéby Modeste Gouissi Armelle sabine Yélignan Hounkpatin Wakili Bolatito Yessoufou Tayéwo Sylvain Biaou Nonvignon Martial Fassinou Laboratory of Ecology Health and Animal Production (LESPA) Faculty of Agronomy (FA) University of Parakou (UP) Parakou Bénin Pluridisciplanary Research Laboratory for Technical Education (LARPET) University of Sciences Technologies Engineering and Mathematics of Abomey (UNSTIM) Lokossa Bénin

This study assessed sediment contamination by heavy metals and pesticide active ingredients linked to chemical inputs used in agricultural activities in the lower Ouémé. Pesticide residues from the organochlorine, pyrethroid and organophosphorus families were investigated by gas chromatography, and heavy metals (Cd, Pb, As, Ni, Zn, Fe, Mg, Cr and Hg) by atomic absorption spectrophotometry. The metallic pollution indices, the contamination factor (CF) and the ecological risk index were calculated. The results revealed 8 active ingredients in the rainy season and 9 in the dry season. Glyphosate was the active ingredient with the highest concentration at all stations, 9.65 ± 0.84 mg/kg recorded in the dry season at the Aguigadji station. All glyphosate values were above the EQS. DDT, Atrazine and Endosulfan also showed high concentrations in the dry and rainy seasons. Emamectin, Abamectin and Lambda Cyhalothrin also showed high concentrations in the dry season at Aguigadji, Ahlan and Sele. Only glyphosate was recorded at the control station (Toho), but in very low concentrations. Lead showed the highest concentrations at all the stations, 265.96 ± 21.02 mg/Kg in the rainy season and 255.38 ± 79.09 mg/Kg in the dry season, all detected at the Aguigadji station and above the EQS. Zn, Ni, Fe, Cu and Cr were all representative in both the dry and rainy seasons at the contaminated stations. Manganese showed high concentrations in the rainy season. Pb showed very high contamination (FC ≥ 6) at the Aguigadji and Ahlan stations and significant contamination (3 ≤ FC 6) at the Sele station in both the rainy and dry seasons. Ni, Hg and Cd, showed either moderate or significant contamination at the contaminated stations. The risk values showed a considerable ecological Ri (190 ≤ Ri < 380) in the rainy season and a moderate ecological Ri (95 ≤ Ri < 190) in the dry season at these contaminated stations.

关键词： Pesticides Heavy Metals Sediments Shrimp Fishing Area Lower Ouémé

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Impact of Dewatering on the Spatio-Temporal Distribution of Benthic Macroinvertebrate Communities in the Okpara River, Benin

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Journal of Geoscience and Environment Protection 2024年第2期12卷 30-43页

作者： Sylvain Tayéwo Biaou Fadéby Modeste Gouissi Armelle sabine Yélignan Hounkpatin Zoulkanerou Orou Piami Wakili Bolatito Yessoufou Souradjou Orou Goura Nonvignon Martial Fassinou Laboratory of Ecology Health and Animal Production (LESPA) Faculty of Agronomy (FA) University of Parakou (UP) Parakou Benin Pluridisciplanary Research Laboratory for Technical Education (LARPET) University of Sciences Technologies Engineering and Mathematics of Abomey (UNSTIM) Lokossa Benin

Surface watercourses are areas of very high ecological and heritage value. Macroinvertebrates are bioindicators of the health of aquatic ecosystems. The aim of this study was to assess the effects of dewatering and re-watering cycles on benthic macroinvertebrate (BMI) communities. Two data collections were carried out at two stations (Okpara 1 and Okpara 2) on the Okpara river before and after dewatering. Thus, 8 samples of benthic macroinvertebrates and 12 physico-chemical parameters (T°C, pH, Transparency, Depth, Conductivity, Dissolved Oxygen that were measured in situ, and BOD5, COD, NH4+, NO3-, NO2- and PO43-) were assayed in the laboratory. Canonical Correspondence Analysis (CCA) was used to match physico-chemical data to MIB families. Shannon and Piélou diversity indices were used to determine the effects of dewatering on MIBs. The increase in temperature values of pH, BOD5, COD, NH4+, NO3-, NO2- and PO43-, after re-watering indicates the effect of dewatering on the quality of Okpara aquatic ecosystems. The benthic macrofauna collected consisted of 62.42% insects, 0.65% crustaceans, 6.48% molluscs, 0.72% worms and 0.14% arachnids. Whereas after re-watering, 21.67% insects, 0% crustaceans, 0.22% molluscs, 7.56% worms and 0.29% arachnids were recorded. Insects, crustaceans and molluscs were more abundant before dewatering than after. This was revealed by low abundances and taxonomic richness, as well as low Shannon index values of samples collected after re-watering.

关键词： Dewatering Benthic Macroinvertebrates Impact Physico-Chemical Parameters Okpara Rivers Nord-Benin

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Agricultural Contamination of the Surface Waters of the Upper Ouémé in Benin: The Case of Heavy Metals and Pesticides

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Journal of Agricultural Chemistry and Environment 2024年第1期13卷 83-99页

作者： Armelle sabine Yélignan Hounkpatin Nonvignon Martial Fassinou Fadéby Modeste Gouissi Zoulkanerou Orou Piami Dossou Armel Géraldo Houndeton Souradjou Orou Goura Wakili Bolatito Yessoufou Tayéwo Sylvain Biaou Laboratory of Hygiene Sanitation Toxicology and Environmental Health (HECOTES) The Training Centre Interfacultaire and Environmental Research for Sustainable Development (CIFRED) of the University of Abomey (UAC) Cotonou Benin Plurildisciplinary Research Laboratory for Technical Education (LARPET) of the University of Sciences Technologies Engineering and Mathematics of Abomey (UNSTIM) Lokossa Benin Laboratoire d’Ecologie de Santé et de Productions Animales (LESPA) Faculté d’Agronomie (FA) Université de Parakou (UP) Parakou Bénin

Objective: The main objective of this study was to assess the degree of contamination of surface waters by heavy metals and pesticides. Method: To this end, data were collected in December 2022 from four specific sampling stations: Okpara, Térou, Affon and Adjiro. Levels of heavy metals, including cadmium, chromium, copper, iron, mercury, nickel and lead, were measured and subjected to in-depth statistical analysis using graphical summation models. In addition, the concentrations of pesticide active ingredients present in the samples were interpreted and evaluated. The statistical data collected during this study were processed using R software, version 3.5.0. Results: The values obtained at the different stations Okpara, Térou, Affon and Adjiro are respectively Arsenic (2 × 10-4 mg/L;2.2 × 10-1 mg/L;1.2 × 10-4 mg/L;2 × 10-4 mg/L), Cadmium (4.4 × 10-5 mg/L;1.1 × 10-2 mg/L;10-4 mg/L;4 × 10-4 mg/L). Then Copper (7 × 10-4 mg/L;3 × 10-3 mg/L;7 × 10-4 mg/L;1 × 10-4 mg/L), Iron (1.51 mg/L;6.4 × 10-1 mg/L;2.0012 mg/L;2.9 × 10-1 mg/L), Lead (0 mg/L;0 mg/L;1.5 × 10-3 mg/L;1.5 × 10-3 mg/L). Mercury, nickel and chromium were not detected in surface waters. It is important to note that the values obtained for trace metals (Cadmium, Chromium, Copper, Iron, Mercury, nickel and chromium were not detected in surface waters. It is important to note that the values obtained for trace metals (cadmium, chromium, copper, iron, mercury, nickel and lead) were all below the guideline standards set by the WHO in 2006 for uncontaminated surface waters. This indicates that the surface waters of the Upper Ouémé were below acceptable contamination thresholds in terms of heavy metals. However, the presence of pesticide active ingredients such as cyfluthrin, endosulfan-alpha, endosulfan-beta, profenosfos, tihan, atrazine, g

关键词： Agricultural Contamination Heavy Metals Pesticides Surface Water North Benin

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Modulation of the in vitro Oxidative Stress and Erythrocyte Cell Membrane Integrity Using Aqueous, Hydroethanolic and Ethanolic Stem-Barks Extracts of Greenwayodendron suaveolens (Engl. & Diels) Verdc

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Pharmacology & Pharmacy 2024年第3期15卷 39-61页

作者： Patrick Hervé Diboue Betote Moustapha Gambo Abdoulaye Francis Ngolsou Esther Del Florence Ndedi Moni Adeline sabine Fanta Yadang Fidel Castro Lah Weyepe Gabriel A. Agbor Nga Nnanga Maximilienne Ascension Nyegue Laboratory of Pharmacology and Drugs Discovery Centre for Research on Medicinal Plants and Traditional Medicine Institute of Medical Research and Medicinal Plants Studies Yaoundé Cameroon Laboratory of Microbiology Department of Microbiology Faculty of Science University of Yaoundé I Yaoundé Cameroon Multidisciplinary Laboratory Department of Galenical Pharmacy and Pharmaceutical Law Faculty of Medicine and Biomedical Sciences University of Yaoundé I Yaoundé Cameroon Department of Physicochemical and Pharmaceutical Sciences Faculty of Health Sciences Abdou Moumouni University Niamey Niger Department of Pharmaceutical and Biological Sciences Faculty of Medicine and Pharmaceutical Sciences University of Douala Douala Cameroon Laboratory of Pharmaceutical Technology Centre for Research on Medicinal Plants and Traditional Medicine Institute of Medical Research and Medicinal Plants Studies Yaoundé Cameroon

Pneumonia, a respiratory infection induces acute or chronic inflammation, characterized by increased activity of lymphocytes and neutrophils, thus generating oxygen-free radicals that decrease the endogenous antioxidants defence system. The aim of this experimental study focused on the capacity of nontoxic aqueous, hydroethanolic and ethanolic extracts of Greenwayodendron suaveolens (Engl. & Diels) Verdc. subsp. suaveolens to regulate free reactive species and protein inflammation generated by infectious disease. The phytochemical screenings of G. suaveolens extracts were carried out according to precipitation and colorimetric methods. The total antioxidant and flavonoid contents were determined by the Folin-Ciocalteu and Aluminium Chloride ethanolic methods. The efficiency of G. suaveolens extracts on free radicals was evaluated using DPPH•, ABTS+•, and FRAP methods. The anti-inflammatory properties of extracts were evaluated according to in vitro protein (BSA) denaturation, Proteinase Inhibitory Action, and Red Blood Cell Membrane stabilization assays. The G. suaveolens aqueous, hydroethanolic and ethanolic extracts were used for the acute toxicity assessment according to the OECD protocol. The obtained results showed the presence of flavonoids, phenols, polyphenols, tannins, anthocyanins, alkaloids, terpenoids, and sterols as secondary metabolites families in G. suaveolens extracts. The highest contents of total antioxidants and flavonoids were highlighted in the hydroethanolic extract. However, it’s the G. suaveolens aqueous extract that showed the best free radical DPPH• and ABTS+• scavenging activities (SC50) of 11.06 μg/mL and 15.16 μg/mL respectively. The highest ferric-reducing activity was found in G. suaveolens ethanolic extract with 866.23 μg EGA/mg of dry weight. The hydroethanolic extract has shown a high anti-inflammatory activity through BSA denaturation and erythrocyte membrane haemolysis with i

关键词： Greenwayodendron suaveolens Secondary Metabolites Oxidative Stress Antioxidant Activity Anti-Inflammatory Properties and Oral Acute Toxicity

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