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Tetracycline-inducible protein expression in pancreatic cancer cells: Effects of CapG overexpression

World Journal of Gastroenterology 2011年第15期17卷 1947-1960页

作者： Sarah Tonack sabina patel Mehdi Jalali Taoufik Nedjadi Rosalind E Jenkins Christopher Goldring John Neoptolemos Eithne Costello Liverpool CR-UK Centre Department of Molecular and Therapeutic Cancer MedicineUniversity of Liverpool Liverpool L69 3GA United Kingdom MRC Centre forDrug Safety Science Department of Pharmacology and Therapeutics School of Biomedical Sciences University of Liverpool L69 3GA United Kingdom National Institute for Health Research Pancreatic Biomedical Research Unit Liverpool L69 3GA United Kingdom

AIM: To establish stable tetracycline-inducible pancreatic cancer cell lines. METHODS: Suit-2, MiaPaca-2, and Panc-1 cells were transfected with a second generation reverse tetracycline-controlled transactivator protein (rtTA2S-M2), under the control of either a cytomegalovirus (CMV) or a chicken β-actin promoter, and the resulting clones were ***: Use of the chicken (β-actin) promoter proved superior for both the production and maintenance of doxycycline-inducible cell lines. The system proved versatile, enabling transient inducible expression of a variety of genes, including GST-P, CYP2E1, S100A6, and the actin capping protein, CapG. To determine the physiological utility of this system in pancreatic cancer cells, stable inducible CapG expressors were established. Overexpressed CapG was localised to the cytoplasm and the nuclear membrane, but was not observed in the nucleus. High CapG levels were associated with enhanced motility, but not with changes to the cell cycle, or cellular proliferation. In CapG-overexpressing cells, the levels and phosphorylation status of other actin-moduating proteins (Cofilin and Ezrin/Radixin) were not altered. However, preliminary analyses suggest that the levels of other cellular proteins, such as ornithine aminotransferase and enolase, are altered upon CapG induction. CONCLUSION: We have generated pancreatic-cancer derived cell lines in which gene expression is fully controllable.

关键词： Pancreatic cancer cells Tetracycline-inducible CapG Suit-2 Panc-1 MiaPaCa-2

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Efficacy of intraovarian autologous platelet-rich plasma administration in women with low ovarian reserve: A systematic review and meta-analysis

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Asian pacific Journal of Reproduction 2024年第2期13卷 60-67页

作者： Yasmin sabina Sa’diah Agung Dewanto Lukman A Chandra Department of Obstetrics and Gynecology Faculty of MedicinePublic Health and NursingUniversitas Gadjah MadaYogyakartaIndonesia Department of Pharmacology and Therapy Faculty of MedicinePublic Health and NursingUniversitas Gadjah MadaYogyakartaIndonesia

Objective:To conduct a systematic examination and meta-analysis of the most reliable data from experimental studies evaluating the efficacy of autologous platelet-rich plasma(PRP)on low ovarian ***:A comprehensive search was performed utilizing pertinent search terms across electronic databases,including PubMed,Cochrane,and Google *** included studies that assigned infertile women with low ovarian reserve in experimental *** reserve parameters were measured before and after PRP injection into *** data of each study was retrieved and subsequently ***:Of 301 articles collected and reviewed,six studies were finally included in the *** PRP injection,infertile women showed a non-significant increase in anti-Mullerian hormone(AMH)level(MD=0.10;95%CI-0.04,0.23),a significant increase in antral follicular count(AFC)(MD=1.88;95%CI 0.47,3.29),and a non-significant reduction in follicle-stimulating hormone(FSH)level(MD=-0.22;95%CI-8.32,7.87).Conclusions:Autologous PRP may increase AFC,but not *** it is found beneficial in enhancing ovarian reserve(AFC),further research with strong evidence is still required.

关键词： Platelet-rich plasma Low ovarian reserve Ovarian rejuvenation

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Correction method for moderate and severe degrees of hallux valgus associated with transfer metatarsalgia

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World Journal of Orthopedics 2024年第3期15卷 238-246页

作者： Amangasy Zhanaspayev Nurlan Bokembayev Marat Zhanaspayev Aidos Tlemissov sabina Aubakirova Alexander Prokazyuk Department of Traumatology and Orthopaedics The National Scientific Center of Traumatology and Orthopaedics Named after Academician Batpenov NDAstana 010000Kazakhstan Department of Traumatology and Paediatric Surgery Non-Commercial Joint-Stock Company“Semey Medical University”Semey 071400Kazakhstan Department of Habilitation and Rehabilitation Center of Habilitation and Rehabilitation of Persons with Disabilities of the Abai RegionSemey 071400Kazakhstan Department of Anaesthesiology and Resuscitation Center of Nuclear Medicine and OncologySemey 071400Kazakhstan

BACKGROUND Hallux valgus(HV)is a common foot deformity that manifests with increasing age,especially in *** associated foot pain causes impaired gait and decreases quality of *** and severe HV is a deformity that is charac-terized by the involvement of lesser rays and requires complex surgical *** this study,we attempted to develop a procedure for this *** To analyse the treatment results of patients who underwent simultaneous surgical correction of all parts of a static forefoot *** We conducted a prospective clinical trial between 2016 and 2021 in which 30 feet with moderate or severe HV associated with Tailor’s bunion and metatarsalgia were surgically treated via a new method involving surgical correction of all associated *** method included a modified Lapidus procedure,M2M3 tarsometatarsal arthrodesis,intermetatarsal fusion of the M4 and M5 bases,and the use of an original external fixation apparatus to enhance correction ***,postoperative,and final follow-up radiographic data and American Orthopaedic Foot and Ankle Society(AOFAS)scores were compared,and P values<0.05 were considered to indicate statistical *** The study included 28 females(93.3%)and 2 males feet(6.7%),20(66.7%)of whom had a moderate degree of HV and 10(33.3%)of whom had severe deformity.M2 and M3 metatarsalgia was observed in 21 feet,and 9 feet experienced pain only at *** mean follow-up duration was 11 *** patients had good correction of the HV angle[preoperative median,36.5 degrees,interquartile range(IQR):30-45;postoperative median,10 degrees,IQR:8.8-10;follow-up median,11.5 degrees,IQR:10-14;P<0.01].At follow-up,metatarsalgia was resolved in most patients(30 vs 5).There was a clinically negligible decrease in the corrected angles at the final follow-up,and the overall AOFAS score was significantly better(median,65 points,IQR:53.8-70;vs 80 points,IQR:75-85;P<0.01).CONCLUSION The developed me

关键词： Hallux valgus Metatarsalgia Tailor’s bunion Lapidus procedure Proximal metatarsal osteotomy Splayfoot

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Fine-needle aspiration technique under endoscopic ultrasound guidance:A technical approach for RNA profiling of pancreatic neoplasms

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2663-2672页

作者： sabina Sherafedinovna Seyfedinova Olga Aleksandrovna Freylikhman Polina Sergeevna Sokolnikova Konstantin Aleksandrovich Samochernykh Anna Aleksandrovna Kostareva Olga Viktorovna Kalinina Evgeniy Gennadievich Solonitsyn Research Laboratory of Digestive Cancer Almazov Medical Research CentreSaint-Petersburg 197341Russia Research Laboratory of Molecular and Cellular Modeling and Gene Therapy Almazov Medical Research CentreSaint-Petersburg 197341Russia Head of Russian Neurosurgical Institute Named after Prof.A.L.Polenova Russian Neurosurgical Institute Named after Prof.A.L.PolenovaSaint-Petersburg 191014Russia Head of Institute of Molecular Biology and Genetics Almazov National Medical Research CentreSaint-Petersburg 197341Russia Research Laboratory of Autoimmune and Autoinflammatory Diseases Almazov National Medical Research CentreSaint-Petersburg 197341Russia

BACKGROUND Early diagnosis of pancreatic ductal adenocarcinoma(PDAC)has been a longstanding *** prognosis of patients with PDAC depends on the stage at *** is necessary to identify biomarkers for the detection and differentiation of pancreatic tumors and optimize PDAC sample preparation procedures for DNA and RNA *** molecular studies are done using paraffin-embedded blocks;however,the integrity of DNA and RNA is often compromised in this ***,RNA isolated from human pancreatic tissue samples is generally of low quality,in part,because of the high concentration of endogenous pancreatic RNAse activity *** To assess the potential of endoscopic ultrasound-guided fine-needle aspiration(EUS-FNA)to obtain specimens from pancreatic neoplasms for subsequent RNA molecular profiling,including next-generation sequencing(NGS).METHODS Thirty-four EUS-FNA samples were included in this study:PDAC(n=15),chronic pancreatitis(n=5),pancreatic cysts(n=14),mucinous cysts(mucinous cystic neoplasia/intraductal papillary mucinous neoplasia)n=7,serous cystic neoplasms n=5,and pseudocysts n=*** material consisted of cyst fluid and cyst wall samples obtained by through-the-needle biopsy(TTNB).Samples were stored at -80℃ until *** purity(A260/230,A260/280 ratios),concentration,and integrity(RIN)were ***-time polymerase chain reaction was conducted on all samples,and small RNA libraries were prepared from solid mass *** RNA was successfully extracted from 29/34(85%)EUS-FNA samples:100% pancreatic adenocarcinoma samples,100% chronic pancreatitis samples,70% pancreatic fluid cyst samples,and 50%TTNB *** relative expression of GAPDH and HPRT were obtained for all successfully extracted RNA samples(n=29)including lowquality RNA *** concentration and nonoptimal RIN values(no less than 3)of RNA extracted from EUS-FNA samples did not prevent NGS library *** suitability of cyst fluid samples

关键词： Endoscopic ultrasound-guided,fine-needle aspiration Pancreatic cancer Pancreatic cysts RNA extraction Through-the-needle biopsy Next-generation sequencing

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Relationship between Seed Priming Mediated Seedling Vigor and Yield Performance of Spring Wheat

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Phyton-International Journal of Experimental Botany 2024年第6期93卷 1159-1177页

作者： Md.Parvez Anwar Masuma Akhter Sharmin Aktar Sinthia Afsana Kheya A.K.M.Mominul Islam sabina Yeasmin Ahmed Khairul Hasan Md.Harun Or Rashid Department of Agronomy Bangladesh Agricultural UniversityMymensingh2202Bangladesh

Seed priming is a pre-germinated technique that can enhance seed germination percentage,faster and synchro-nized germination,better seedling growth,and yield under stress *** ascertain the most effective seed priming method that would ensure the potential yield of wheat in Bangladesh,two experiments were carried out from December 2021 to March 2022 at the Department of Agronomy,Bangladesh Agricultural *** wheat varieties namely BARI Gom-28 and BWMRI Gom-1 were subjected to a range of priming chemicals in both lab and pot *** compounds included the following:control(no priming),hydropriming(distilled water),10000 ppm KNO_(3),15000 ppm KNO_(3),40000 ppm Mannitol,60000 ppm Mannitol,10000 ppm NaCl,20000 ppm NaCl,100 ppm PEG,150 ppm PEG,500 ppm NaOCl,1000 ppm NaOCl,10000 ppm CaCl_(2),20000 ppm CaCl_(2),10000 ppm KCl and 20000 ppm KCl.A complete randomized design(CRD)with three repli-cations was used to set up the *** results showed that BARI Gom-28 and BWMRI Gom-1 responded best to KCl priming in terms of rapid seed germination and strong seedling *** the other hand,the best priming agents for plant growth and productivity turned out to be CaCl_(2) and *** results of this study support the possibility of using seed priming as a technique to improve wheat plant development and output by raising seed emergence and survival rates.

关键词： Priming seedling percentage germination growth yield wheat

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中小企业如何实现数字化技术

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现代制造 2024年第1期 24-26页

作者： sabina Merk 不详

一段时间以来,中小企业希望实现数字化的势头非常强劲。因为数字化技术带来的好处是显而易见的。本文将介绍如何用合理的解决方案理想地帮助中小企业实现数字化工厂的转型。当前有两种相互矛盾的想法影响着企业的生产情况:许多公司的订... 详细信息

一段时间以来,中小企业希望实现数字化的势头非常强劲。因为数字化技术带来的好处是显而易见的。本文将介绍如何用合理的解决方案理想地帮助中小企业实现数字化工厂的转型。当前有两种相互矛盾的想法影响着企业的生产情况:许多公司的订单已满,但又不想在投资巨大的情况下更好地利用现有的生产能力。

关键词：中小企业数字化工厂数字化技术生产能力相互矛盾解决方案公司显而易见

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Adverse events related to physical restraint use in intensive care units: A review of the literature

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Journal of Intensive Medicine 2024年第3期4卷 318-325页

作者： Sebastian Berger Pascale Grzonka Simon A.Amacher sabina Hunziker Anja I.Frei Raoul Sutter Clinic for Intensive Care Department of Acute MedicineUniversity Hospital BaselBaselSwitzerland Medical Communication and Psychosomatic Medicine University Hospital BaselBaselSwitzerland Medical faculty University of BaselBaselSwitzerland Department of Clinical Research University of BaselBaselSwitzerland

Physical restraints are widely used and accepted as protective measures during treatment in intensive care unit (ICU). This review of the literature summarizes the adverse events and outcomes associated with physical restraint use, and the risk factors associated with their use during treatment in the ICU. The PubMed, Scopus, and Google Scholar databases were screened using predefined search terms to identify studies pertaining to adverse events and/or outcomes associated with physical restraint use, and the factors associated with their use in adult patients admitted to the ICU. A total of 24 articles (including 6126 patients) that were published between 2006 and 2022 were identified. The described adverse events associated with physical restraint use included skin injuries, subsequent delirium, neurofunctional impairment, and a higher rate of post-traumatic stress disorder. Subsequent delirium was the most frequent adverse event to be reported. No alternative measures to physical restraints were discussed, and only one study reported a standardized protocol for their use. Although physical restraint use has been reported to be associated with adverse events (including neurofunctional impairment) in the literature, the available evidence is limited. Although causality cannot be confirmed, a definite association appears to exist. Our findings suggest that it is essential to improve awareness regarding their adverse impact and optimize approaches for their detection, management, and prevention using protocols or checklists.

关键词： Physical restraint Intensive care unit Delirium Complication Adverse event

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Production of Ryazhenka,a traditional Ukrainian fermented baked milk,by using electro-activated whey as supplementing ingredient and source of lactulose

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Food Bioscience 2022年第2期46卷 221-230页

作者： sabina Aidarbekova Mohammed Aider Department of Food Sciences Universit´e LavalQuebecQCG1V 0A6Canada Institute of Nutrition and Functional Foods INAF Universit´e LavalQuebecQCG1V 0A6Canada Department of Soil Sciences and Agri-food Engineering Universit´e LavalQuebecQCG1V 0A6Canada

In this work,electro-activated whey(EAW)was used as ingredient in the preparation of Ryazhenka,a fermented baked milk,at a supplementation level of 3 and 9%.Fermentation kinetics,viscosity,color,antioxidant capacity,and protein profiles of the final product were ***,effect of electro-activation onβ-lactoglobulin allergenicity was evaluated by a specific ELISA *** results showed that addition of electro-activated whey as supplementing ingredient is technologically feasible in the process of Ryazhenka *** fermentation kinetics showed that this process was in the technologically accepted interval even if the addition of EAW delayed the fermentation *** viscosity study showed that addition of EAW permitted to obtain a final product with good followability corresponding to good consistency of drinkable dairy ***,addition of EAW prevented syneresis during the refrigerated storage at 4℃ and enriched the product with lactulose which is a recognized *** chromatic evaluation of the final product showed a compatibility of EAW with Ryazhenka which is known for its specific yellowish-brownish *** or EAW significantly improved the antioxidant capacity of the Ryazhenka with values of IC_(50)DPPH=2.54±0.02 and IC_(50)PFRAP=244.03±28.83 for the Ryazhenka produced by adding 9%EAW to the milk after the baking process and prior to ***,by using aβ-lactoglobulin specific ELISA test,this study showed a significant decrease of whey and pureβ-lactoglobulin allergenicity by 95%.This decrease was also observed in Ryazhenka supplemented with 9%EAW but at lower ***,it can be concluded that electro-activated whey can be considered as a promising functional ingredient containing a prebiotic lactulose as functional ingredient in the dairy industry.

关键词： Electro-activated whey Ryazhenka Lactulose Prebiotic Allergenicity reduction

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Clinical and experimental research in antituberculosis drug-induced hepatotoxicity:a review

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Journal of Integrative Medicine 2017年第1期15卷 27-36页

作者： Udhaya Lavinya Baskaran Evan Prince sabina School of Biosciences and Technology VIT University Vellore-632014 Tamilnadu India

Drug-induced liver injury is the common adverse effect seen in patients receiving antituberculosis drugs （ATDs）. There are several risk factors associated with the development of hepatotoxicity in such patients. Though there have been appreciable efforts taken by carrying out studies investigating the efficacy of several natural and synthetic compounds in minimising this effect, the only choice available for clinicians is withdrawal of drugs. This review would give a precise idea of ATD-induced hepatotoxicity its underlying mechanisms and alternative therapies for the same.

关键词： antitubercular agents isoniazid rifampicin pyrazinamide oxidative stress complementarytherapies

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Perspectives from a publishing ethics and research integrity team for required improvements

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数据与情报科学学报:英文版 2023年第3期8卷 1-14页

作者： sabina Alam Laura Wilson Publishing Ethics and Research Integrity Taylor and Francis GroupJournalsUK

It is imperative that all stakeholders within the research ecosystem take responsibility to improve research integrity and reliability of published *** on the unique experiences of a specialist publishing ethics and research integrity team within a major publisher,this article provides insights into the observed trends of misconduct and how those have evolved over time,and addresses key actions needed to improve the interface between researchers,funders,institutions and publishers to collectively improve research integrity on a global scale.

关键词： Research integrity Publishing ethics Training Education

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