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Methods for in vitro evaluating antimicrobial activity： A review

Journal of Pharmaceutical Analysis 2016年第2期6卷 71-79页

作者： mounyr balouiri moulay sadiki saad koraichi ibnsouda Laboratory of Microbial Biotechnology Faculty of Sciences and Techniques University Sidi Mohamed Ben Abdellah B.P.. 2202 lmouzzer Road Fez Morocco

In recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Several bioassays such as disk-diffusion, well diffusion and broth or agar dilution are well known and commonly used, but others such as flow cy- tofluorometric and bioluminescent methods are not widely used because they require specified equip- ment and further evaluation for reproducibility and standardization, even if they can provide rapid re- sults of the antimicrobial agent＇s effects and a better understanding of their impact on the viability and cell damage inflicted to the tested microorganism. In this review article, an exhaustive list of in vitro antimicrobial susceptibility testing methods and detailed information on their advantages and limita- tions are reported.

关键词： Thin-layer chromatography (TLC)-bioautography Time-kill test Antimicrobial gradient method Agar diffusion method

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Microbiologically influenced corrosion of 304L stainless steel caused by an alga associated bacterium Halomonas titanicae

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Journal of Materials Science & Technology 2020年第2期37卷 200-206页

作者： Yuqiao Dong Yassir Lekbach Zhong Li Dake Xu Soumya El Abed saad ibnsouda koraichi Fuhui Wang Shenyang National Laboratory for Materials Science Northeastern UniversityShenyang 110819China Center for Marine Materials Corrosion and Protection College of MaterialsXiamen UniversityXiamen 361005China Laboratory of Microbial Biotechnology Faculty of Science and TechnologySidi Mohamed Ben Abdellah UniversityB.P.2202FezMorocco

Algae are reported to be corrosive,while little is known about the role of the algae associated bacteria in the corrosion *** the present study,Halomonas titanicae was isolated from a culture of an alga strain,Spirulina platensis,and identified through 16 S rRNA gene *** behavior of 304L stainless steel(SS)coupons in the presence and absence of *** was characterized by using electrochemical measurements and surface *** results showed that *** significantly accelerated the corrosion rate and decreased the pitting potential of 304L SS in the biotic *** removal of the corrosion products and biofilms,severe pitting corrosion caused by *** was *** largest pit depth after 14 d reached 6.6μm,which was 5.5 times higher than that of the sterile control(1.2μm).This is the first report revealing that an alga associated bacterium can induce microbiologically influenced corrosion(MIC),and a further concern is raised that whether algae play a role in the MIC process.

关键词： Microbiologically influenced corrosion Halomonas titanicae Pitting corrosion

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Quantitative Adhesion of <i>Staphylococcus aureus</i>on Stainless Steel Coated with Milk

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Food and Nutrition Sciences 2013年第3期4卷 299-304页

作者： Fatima Hamadi Hassan Latrache Fatima Asserne Soumya Elabed Hafida Zahir ibnsouda koraichi saad Hafida Hanine Jamaa Bengourram Laboratory of Biotechnology and Vaorization of Natural Resources Department of Biology Faculty of Science University Ibn Zohr Agadir Marocco Laboratory of industrial Engineering Food and Environment Faculty of Sciences and Technics University Sultan Moulay Slimane Beni Mellal Marocco Laboratory of Valorization and Safety of Food Products Faculty of Sciences and Technics University Sultan Moulay Slimane Beni Mellal Morocco Laboratoy of Microbial Biotechnology Faculty of Science and Technics University Sidi Mohamed Ben Abdellah Fés-Sáis Marocco

The surface energy characteristics of uncoated (clean) and coated stainless steel with UHT milk at various contact time (5 min, 30 min, 1 hours, 3 hours, 6 hours, 24 hours) were determined using contact angle measurement. Whatever the contact time, the clean stainless steel coupons became more hydrophobic and more electron acceptor when they are coated by milk. Inversely, the electron donor character seems to decreasing in this condition. The calculated surface energy component of coated stainless steel was found to vary with contact time. Its hydrophobicity and its electron acceptor were minimal after 3 hours of contact, but its electron donor was minimal after 1 hours of contact. Adhesion experiments of Staphylococcus aureus were carried out on uncoated and coated stainless steels at various contact times. For all contact times, the adhesion results show that milk reduce S. aureus adhesion, and the level of this reduction depend on contact time. This reduction was lower and higher after 1 hours, 5 min and 30 min of contact respectively.

关键词： Surface Energy Characteristics S. aureus Adhesion Milk Stainless Steel

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