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Unusual substructure conformations observed in crystal structures of a dicistrovirus RNA-dependent RNA polymerase suggest contribution of the N-terminal extension in proper folding

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Virologica Sinica 2023年第4期38卷 531-540页

作者： Xiang Fang Guoliang Lu Yanchun deng sa Yang Chunsheng Hou Peng Gong Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyChinese Academy of SciencesWuhan430207China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha410205China University of Chinese Academy of Sciences Beijing100049China Institute of Apicultural Research Chinese Academy of Agricultural SciencesBeijing100193China Hubei Jiangxia Laboratory Wuhan430207China

The dicistroviridae is a virus family that includes many insect *** viruses contain a positive-sense RNA genome that is replicated by the virally encoded RNA-dependent RNA polymerase(RdRP)also named 3d^(pol).Compared with the Picornaviridae RdRPs such as poliovirus(PV)3d^(pol),the dicistroviridae representative Israeli acute paralysis virus(IAPV)3d^(pol) has an additional N-terminal extension(NE)region that is about 40-residue in *** date,both the structure and catalytic mechanism of the dicistroviridae RdRP have remain *** we reported crystal structures of two truncated forms of IAPV 3d^(pol),namelyΔ85 andΔ40,both missing the NE region,and the 3d^(pol) protein in these structures exhibited three conformational *** palm and thumb domains of these IAPV 3d^(pol) structures are largely consistent with those of the PV 3d^(pol) ***,in all structures,the RdRP fingers domain is partially disordered,while different conformations of RdRP substructures and interactions between them are also *** particular,a large-scale conformational change occurred in the motif B-middle finger region in one protein chain of theΔ40 structure,while a previously documented alternative conformation of motif A was observed in all IAPV *** experimental data on one hand show intrinsic conformational variances of RdRP substructures,and on the other hand suggest possible contribution of the NE region in proper RdRP folding in IAPV.

关键词： Israeli acute paralysis virus(IAPV) Poliovirus RNA-dependent RNA polymerase(RdRP) Crystal structure Catalytic motif

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decreasing recurrent bowel obstructions,improving quality of life with physiotherapy:Controlled study

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World Journal of Gastroenterology 2018年第19期24卷 2108-2119页

作者： Amanda d Rice Kimberley Patterson Evette d Reed Belinda F Wurn Kristen Robles Bernhard Klingenberg Leonard B Weinstock Janey sa Pratt C Richard King III Lawrence J Wurn department of Internal Medicine College of Medicine-PhoenixUniversity of ArizonaPhoenixAZ 85004United States Clear Passage Therapies GainesvilleFL 32606United States department of Mathematics and Statistics Williams CollegeWilliamstownMA 01267United States Clinical Medicine and Surgery Washington University School of MedicineSpecialists in GastroenterologyLLCSt.LouisMO 63141United States department of Surgery Stanford University School of MedicinePalo AltoCA 94035United States College of Medicine University of FloridaGainesvilleFL 32607United States

AIM To compare(1) quality of life and(2) rate of recurrent small bowel obstructions(SBO) for patients treated with novel manual physiotherapy vs no treatment. METHOdS One hundred and three subjects(age 19-89) with a history of recurrent adhesive SBO were treated with a manual physiotherapy called the Clear Passage Approach(CPA) which focused on decreasing adhesive crosslinking in abdominopelvic viscera. Pre-and post-therapy data measured recurring obstructions and quality of life, using a validated test sent 90 d after therapy. Results were compared to 136 untreated control subjects who underwent the same measurements for subjects who did not receive any therapy, which is the normal course for patients with recurring SBO. Comparison of the groups allowed us to assess changes when the physiotherapy was added as an adjunct treatment for patients with recurring *** despite histories of more prior hospitalizations, obstructions, surgeries, and years impacted by bowel issues, the 103 CPA-treated subjects reported a significantly lower rate of repeat SBO than 136 untreated controls(total obstructions P = 0.0003; partial obstructions P = 0.0076). Subjects treated with the therapy demonstrated significant improvements in five of six total domains in the validated Small Bowel Obstruction Questionnaire(SBO-Q). domains of diet, pain, gastrointestinal symptoms, quality of life(QOL) and pain severity when compared to post CPA treatment were significantly improved(P dication domain was not changed in the CPA treated group(P = 0.176).CONCLUSION CPA physical therapy was effective for patients with adhesive SBO with significantly lower recurrence rate, improvement in reported symptoms and overall quality of life of subjects.

关键词： Clear passage approach Manual therapy Physical therapy Small bowel obstructions Adhesions Physiotherapy Alternative therapy

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智能化三维模拟重建教学法在老年弥漫性肺部疾病医学影像教学中的应用

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国际老年医学杂志 2024年第3期45卷 372-375页

作者：矫鑫瑶陈鹏宋涵黄萨吉林大学第二医院放射线科长春130041 吉林大学第二医院儿科长春130041

本研究以主要发病于老年人的弥漫性肺部疾病(dLd)为例,基于ITK-snap软件实施医学影像教学的改革创新。在教学过程中尝试通过智能化三维模拟重建肺部CT影像,借助线上与线下相融合的教学模式从而融合临床表现、影像、病理、生理以及药理... 详细信息

本研究以主要发病于老年人的弥漫性肺部疾病(dLd)为例,基于ITK-snap软件实施医学影像教学的改革创新。在教学过程中尝试通过智能化三维模拟重建肺部CT影像,借助线上与线下相融合的教学模式从而融合临床表现、影像、病理、生理以及药理等多学科内容。该教学模式的创新之处在于以临床医学专业学生为研究对象,将学生置于教学活动中的主体位置,探讨智能化三维模拟重建教学法在研究生dLd影像教学中的意义,进而提高学生在学习上的主动性并培养学生临床思维能力。

关键词：医学影像学三维模拟重建 ITK-snap软件教学

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Emotion processing in Parkinson's disease: a blood oxygenation level-dependent functional magnetic resonance imaging study

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Neural Regeneration Research 2019年第4期14卷 666-672页

作者： Mohammed Benzagmout sa.d Boujraf Badreeddine Alami Hassane Ali Amadou Halima El Hamdaoui Amine Bennani Mounir Jaafari Ismail Rammouz Mustapha Maaroufi Rabia Magoul driss Boussaoud Laboratory of Clinical Neuroscience Faculty of Medicine department of Neurosurgery University Hospital of Fez Laboratory of Neuroendocrinology and Nutritional and Climatic Environment Faculty of Sciences Dhar El Mahraz department of Biophysics and Clinical MRI Methods Faculty of Medicine department of Radiology and Clinical Imaging University Hospital of Fez department of Psychiatry University Hospital of Fez Aix Marseille Université INSERM INS Institut de Neurosciences des Systèmes

Parkinson's disease is a neurodegenerative disorder caused by loss of dopamine neurons in the substantia nigra pars compacta. Tremor, rigidity, and bradykinesia are the major symptoms of the disease. These motor impairments are often accompanied by affective and emotional dysfunctions which have been largely studied over the last decade. The aim of this study was to investigate emotional processing organization in the brain of patients with Parkinson's disease and to explore whether there are differences between recognition of different types of emotions in Parkinson's disease. We examined 18 patients with Parkinson's disease(8 men, 10 women) with no history of neurological or psychiatric comorbidities. All these patients underwent identical brain blood oxygenation level-dependent functional magnetic resonance imaging for emotion evaluation. Blood oxygenation level-dependent functional magnetic resonance imaging results revealed that the occipito-temporal cortices, insula, orbitofrontal cortex, basal ganglia, and parietal cortex which are involved in emotion processing, were activated during the functional control. Additionally, positive emotions activate larger volumes of the same anatomical entities than neutral and negative emotions. Results also revealed that Parkinson's disease associated with emotional disorders are increasingly recognized as disabling as classic motor symptoms. These findings help clinical physicians to recognize the emotional dysfunction of patients with Parkinson's disease.

关键词： Parkinson’s disease emotion processing Blood oxygenation level-dependent functional magnetic resonance imaging brain activation dopamine neural regeneration

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石蒜种子萌发过程及生理研究

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江西农业大学学报 2024年第3期46卷 682-690页

作者：萨日娜蔡军火游欣温婷姜雪茹魏绪英钟良涛江西农业大学园林与艺术学院江西南昌330045 江西财经大学艺术学院江西南昌330032

【目的】探究石蒜种子萌发过程中的形态与生理变化规律,为其优质种苗的繁育提供理论基础及高效繁殖技术。【方法】以成熟新鲜的石蒜种子为研究材料,在观测其萌发第0、7、14、21、28、35天形态变化的基础上,采用考马斯亮蓝G-250法、蒽酮... 详细信息

【目的】探究石蒜种子萌发过程中的形态与生理变化规律,为其优质种苗的繁育提供理论基础及高效繁殖技术。【方法】以成熟新鲜的石蒜种子为研究材料,在观测其萌发第0、7、14、21、28、35天形态变化的基础上,采用考马斯亮蓝G-250法、蒽酮比色法、酶联免疫法测定种子内的可溶性蛋白质、可溶性糖和淀粉、内源激素(GA_(3)、ABA、IAA、ZR)等内含物。【结果】(1)石蒜种子的萌发方式为子叶留土型,依据形态变化特征,可将其萌发过程划分为未萌发期(0 d)、露白期(7~14 d)、子叶伸长期(8~14 d)、根快速生长期(15~21 d)、分根期(22~28 d)和真叶形成期(29~35 d)6个时期,持续时长约35 d。(2)在种子萌发过程中,淀粉、可溶性蛋白、可溶性糖、内源ABA的含量及GA_(3)/ABA比值总体均呈“下降”趋势,IAA和ZR的含量却呈“上升”趋势,GA_(3)含量则“先降后升”;其中,子叶伸长期和根快速生长期是内源激素变化的拐点时期。(3)种子的萌发进程与ZR、IAA的含量变化呈极显著正相关,而与GA_(3)、ABA的含量变化则呈显著负相关。高含量的IAA和ZR、低含量的ABA和GA_(3)有利于胚乳内淀粉的水解,从而促进种子的萌发。【结论】石蒜种子的萌发是一个动态耗能过程,主要依赖胚乳内的淀粉和可溶性糖提供能量。同时,种子的萌发也受到内源激素ZR和IAA的正向调控及GA_(3)/ABA的负向调控。

关键词：石蒜种子萌发淀粉可溶性糖可溶性蛋白质内源激素

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Genetic differentiation in functional traits among wild cherry (Prunus avium L.) half-sib lines

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Journal of Forestry Research 2022年第3期33卷 991-1003页

作者： Srđan Stojnić Branislav Kovačević Marko Kebert Verica Vasić Vanja Vuksanović Branislav Trudić saša Orlović Institute of Lowland Forestry and Environment University of Novi SadAntonaČehova 13dNovi Sad21000Serbia Faculty of Agriculture University of Novi SadTrg Dositeja Obradovića 8Novi Sad21000Serbia Food and Agriculture Organization of the United Nations Viale delle Terme di Caracalla00153RomeItaly

Understanding intra-specific variation in leaf functional traits is one of the key requirements for the evaluation of species adaptive capacity to ongoing climate change, as well as for designing long-term breeding and conservation strategies. Hence, data of 19 functional traits describing plant physiology, antioxidant properties, anatomy and morphology were determined on 1-year-old seedlings of wild cherry (Prunus avium L.) half-sib lines. The variability within and among half-sib lines, as well as the estimation of multi-trait association, were examined using analysis of variance (ANOVA) followed by Tukey's honestly significant difference test and multivariate analyses: principal component analysis (PCA), canonical discriminant analysis (CdA) and stepwise discriminant analysis (SdA). Pearson’s correlation coefficient was used to evaluate linear correlation between the study parameters. The results of the ANOVA showed the presence of statistically significant differences (P dy traits. The differences within half-sib lines, observed through the contribution of the examined sources of variation to the total variance (%), had higher impact on total variation in the majority of the examined traits. Pearson’s correlation analysis and PCA showed strong relationships between gas exchange in plants and leaf size and stomatal density, as well as between leaf biomass accumulation, intercellular CO_(2) concentration and parameters related to antioxidant capacity of plants. Likewise, the results of SdA indicate that transpiration and stomatal conductance contributed to the largest extent, to the discrimination of the wild cherry half-sib lines. In addition, PCA and CdA showed separation of the wild cherry half-sib lines along the first principal component and first canonical variable with regards to humidity of their original sites. Multiple adaptive differences between the wild cherry half-sib lines indicate high potential of the specie

关键词： Wild cherry Common garden experiment Variability Multivariate statistics Leaf functional traits

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Coprophilous contributions to the phylogeny of Lasiosphaeriaceae and allied taxa within Sordariales(Ascomycota,Fungi)

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Fungal diversity 2015年第1期70卷 101-113页

作者： Åsa Kruys sabine M.Huhndorf Andrew N.Miller Systematic Biology Department of Organismal BiologyEvolutionary Biology CentreUppsala UniversityNorbyvägen 18DSE-75236 UppsalaSweden Botany department Field MuseumChicagoIL 60605USA Illinois Natural History Survey University of IllinoisChampaignIL 61820US

The phylogenetic relationships of Lasiosphaeriaceae are complicated in that the family is paraphyletic and includes Sordariaceae and Chaetomiaceae,as well as several polyphyletic *** study focuses on the phylogenetic relationships of the coprophilous genera,Anopodium,Apodospora,Arnium,Fimetariella and *** are traditionally circumscribed based on ascospore characters,which have proven homoplasious in other genera within the *** results based on LSU nrdNA andß–tubulin sequences distinguish four lineages of Lasiosphaeriaceae *** joins the clade of mor-phologically similar,yellow-pigmented species of Cercophora and *** is monophyletic and joins a larger group of taxa with unclear affinities to each other,while Arnium is polyphyletic being scattered throughout three of the four major clades of *** is represented by a single collection and joins the clade containing Cercophora scortea and Podospora *** shows affinities to the Lasiosphaeris *** on a combination of morphological and molecular data,Echria *** recognized at the genus level for the former Arnium section and two new combinations are proposed:*** and ***.

关键词： Ascomal wall Ascomycete β-tubulin LSU nrdNA Systematics,Taxonom

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Three-dimensional,isotropic imaging of mouse brain using multi-view deconvolution light sheet microscopy

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Journal of Innovative Optical Health Sciences 2017年第5期10卷 94-100页

作者： sa Liu Jun Nie Yusha Li Tingting Yu dan Zhu Peng Fei School of Optical and Electronic Information Huazhong University of Science and Technology Wuhan 430074 P.R.China Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 P.R.China

We present a threedimensional(3d)isotropic imaging of mouse brain using light-sheet fuo-rescent microscopy(LSFM)in conjumction with a multi-view imaging *** common single view LSFM is used for mouse brain imaging,the brain tissue is 3d imaged under eight views in our study,by a home-built selective plane ilumination microscopy(SPIM).An output image containing complete structural infornation as well as significantly improved res olution(~4 times)are then computed based on these eight views of data,using a bead-guided multi-view registration and *** superior imaging quality,the astrocyte and pyrarmidal neurons together with their subcellular nerve fbers can be clearly visualized and *** further incuding other computational methods,this study can be potentially scaled up to map the conectome of whole mouse brain with a simple *** microscope.

关键词： Light sheet fuorescent microscopy multi-view dconvolution mouse brain imaging isotropic

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影像三维重建安全辅助单孔分体内镜治疗L_(5)/S_(1)极外侧腰椎间盘突出症

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中国组织工程研究 2025年第9期29卷 1876-1882页

作者：冯志萌孙宁孙兆忠李岳飞刘昌震李洒滨州医学院附属医院脊柱外科山东省滨州市256603

背景:单孔分体内镜作为一种新型的内镜技术,适用于治疗极外侧腰椎间盘突出症。但目前国内外针对L_(5)/S_(1)这一发病率极低的极外侧腰椎间盘突出症的研究资料甚少,尚无详尽描述单孔分体内镜治疗L_(5)/S_(1)极外侧腰椎间盘突出症的影像... 详细信息

背景:单孔分体内镜作为一种新型的内镜技术,适用于治疗极外侧腰椎间盘突出症。但目前国内外针对L_(5)/S_(1)这一发病率极低的极外侧腰椎间盘突出症的研究资料甚少,尚无详尽描述单孔分体内镜治疗L_(5)/S_(1)极外侧腰椎间盘突出症的影像解剖学资料。目的:通过影像三维重建确定骨性标志点,并以该标志点准确定位L_(5)出口神经根、L_(5)/S_(1)椎间隙及其他结构之间的位置关系,辅助单孔分体内镜经后外侧入路实现对L_(5)出口神经根的减压,治疗L_(5)/S_(1)极外侧腰椎间盘突出症。方法:选择符合纳入标准的29例L_(5)/S_(1)单侧极外侧腰椎间盘突出症患者,其中男12例,女17例;年龄48-74岁。将患者的腰椎CT数据资料导入Mimics 21.0软件中重建腰椎三维模型。测量L_(5)/S_(1)相关参数:①在横突根部下缘与峡部外侧缘的交点(H)所在矢状面上测量:H分别至L_(5)出口神经根上缘、下缘的垂直距离(a1,a2);H分别至L_(5)下终板、S1上终板的垂直距离(b1,b2);H至L_(5)椎弓根下缘的垂直距离(c);②H至L_(5)椎弓根内侧壁所在矢状面的左右水平距离(d);③H至硬脊膜外侧缘所在矢状面的左右水平距离(e);④H至L_(5)下终板最外侧缘所在矢状面的左右水平距离(f);⑤在L_(5)下终板最外侧缘所在矢状面上测量:H所在横断面分别至L_(5)出口神经根上缘、下缘的垂直距离(g1,g2);H所在横断面分别至L_(5)下终板、S1上终板的垂直距离(h1,h2);⑥H至L_(5)出口神经根最后缘所在冠状面的前后水平距离(i);⑦骶骨翼后缘最高点至L_(5)下终板最后缘所在冠状面的前后水平距离(j)。结果与结论:①男女之间各相关测量参数相比差异无显著性意义(P>0.05);②a1,a2,b1,b2,c,d,e,f,h1,h2,g1,g2,i,j患侧与健侧相比差异无显著性意义(P>0.05);③观测发现,a1与c之间相比差异无显著性意义(P>0.05),表明椎弓根下缘即为L_(5)出口神经根上缘,L_(5)出口神经根紧贴椎弓根下缘并经L_(5)椎体后方向前外侧走行,H均位于L_(5)出口神经根上方;④以H作为骨性标志点,无需向上探查,无需磨除峡部,只需向下、向外侧磨除部分骨质显露L_(5)出口神经根与椎间隙,向内侧有足够的安全距离避免损伤硬脊膜来完成侧隐窝、椎间孔区域的探查减压;⑤术者可在H、L_(5)下终板最外侧缘所在的矢状面及L_(5)横突与骶骨翼构成的“矩形区域”内进行操作,越靠近内下方区域(Kambin三角)越安全;⑥提示单孔分体内镜经后外侧入路治疗L_(5)/S_(1)极外侧腰椎间盘突出症,在镜下以H作为骨性标志点定位L_(5)出口神经根及椎间隙,能实现对L_(5)出口神经根的精准、安全、有效减压。

关键词：极外侧腰椎间盘突出症单孔分体内镜影像三维重建骨性标志数字化软件

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HYPERSINGULAR INTEGRAL EQUATIONS FOR PERIOdIC ARRAYS OF PLANAR CRACKS IN A PERIOdICALLY-LAYEREd ANISOTROPIC ELASTIC SPACE UNdER ANTIPLANE SHEAR STRESS

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Acta Mathematica Scientia 1999年第3期19卷 343-355页

作者： W.T.Ang (Faculty of Engineering, Universiti Malaysia sarawak, 94300 Kota samarahan, Malaysia)d.L. Clements(department of Applied Mathematics, University of Adelaide, sa5005, Australia) Faculty of Engineering Universiti Malaysia Sarawak 94300 Kota Samarahan Malaysia department of Applied Mathematics University of Adelaide SA5005 Australia

Hypersingular integral equations are derived for the problem of determining the antiplane shear stress around periodic arrays of planar cracks in a periodically-layered anisotropic elastic space. The unknown functions are directly related to the jump in the displacements across opposite crack faces. Once the integral equations are solved, crack parameters of interest, such as the clack tip stress intensity factors, may be readily *** some specific examples of the problem, the integral equations are solved numerically by using a collocation technique, in order to compute the relevant stress intensity factors.

关键词： Periodically-located cracks periodically-layered anisotropic material antiplane shear stress hypersingulal integral equations

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