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医学生信息素养现状分析及提升对策

济宁医学院学报 2024年第5期47卷 448-452页

作者：宋国建高立孙娟刘昕王倩飞济宁医学院信息技术中心日照276826 济宁医学院期刊管理中心济宁272013 济宁医学院医学信息工程学院日照276826

随着信息技术的迅猛发展和医学行业的不断进步,信息素养已经成为医学生必备的核心能力之一。然而,当前医学生的信息素养亟须提高,存在信息意识不强、信息能力薄弱、信息检索和利用技能欠缺等问题,不仅影响了医学生获取、分析和利用医学... 详细信息

随着信息技术的迅猛发展和医学行业的不断进步,信息素养已经成为医学生必备的核心能力之一。然而,当前医学生的信息素养亟须提高,存在信息意识不强、信息能力薄弱、信息检索和利用技能欠缺等问题,不仅影响了医学生获取、分析和利用医学信息的效率和质量,更限制了其在学术研究和临床实践中的发展。通过有针对性地提出完善信息素养教育课程体系、加强师资队伍建设、营造良好的学习氛围等具体对策,不仅可以有效提升医学生的信息检索、筛选、评估和利用等能力,使医学生能够更好地适应信息化时代的医学发展需求,还可为医学院校提升信息素养教育质量、培养高质信息素养的医学人才提供有益参考。

关键词：医学生信息素养信息现状对策

Characterization of Genome-Wide Microsatellites of Saccharina japonica Based on a Preliminary Assembly of Illumina Sequencing Reads

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Journal of Ocean University of China 2016年第3期15卷 523-532页

作者： ZHANG Linan PENG Jie LI Xiaojie CUI Cuiju sun juan YANG Guanpin Marine Science and Engineering College Qingdao Agriculture UniversityQingdao 266109P.R.China Shandong Oriental Ocean Sci-tech Co. Ltd National Engineering and Technique Research and Development Center of Algae and Sea Cucumber of China Provincial Key Laboratory of Genetic Improvement and Efficient Culture of Marine Algae of Shandong Yantai 264003P.R.China College of Marine Life Sciences Ocean University of ChinaQingdao 266003P.R.China

Microsatellites or simple sequence repeats(SSR) function widely and locate dependently in genome. However, their characteristics are often ignored due to the lack of genomic sequences of most species. Kelp(Saccharina japonica), a brown macroalga, is extensively cultured in China. In this study, the genome of S. japonica was surveyed using an Illumina sequencing platform, and its microsatellites were characterized. The preliminarily assembled genome was 469.4 Mb in size, with a scaffold N_(50) of 20529 bp. Among the 128370 identified microsatellites, 90671, 25726 and 11973 were found in intergenic regions, introns and exons, averaging 339.3, 178.8 and 205.4 microsatellites per Mb, respectively. These microsatellites distributed unevenly in S. japonica genome. Mononucleotide motifs were the most abundant in the genome, while trinucleotide ones were the most prevalent in exons. The microsatellite abundance decreased significantly with the increase of motif repeat numbers, and the microsatellites with a small number of repeats accounted for a higher proportion of the exons than those of the intergenic regions and introns. C/G-rich motifs were more common in exons than in intergenic regions and introns. These characteristics of microsatellites in S. japonica genome may associate with their functions, and ultimately their adaptation and evolution. Among the 120140 pairs of designed microsatellite primers, approximately 75% were predicted to be able to amplify S. japonica DNA. These microsatellite markers will be extremely useful for the genetic breeding and population evolution studies of kelp.

关键词： Saccharinajaponica genome microsatellite characterization

Hydrothermal Synthesis and Luminescent Properties of Flowerlike Zn_2GeO_4 and Mn^(2+)-Doped Zn_2GeO_4 Hierarchical Architectures

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Chemical Research in Chinese Universities 2012年第5期28卷 764-767页

作者： LI Bo SONG Shu-yan sun Xiu-juan PENG Jing WANG Bo XING Yan College of Chemistry Northeast Normal UniversityChangchun 130024P.R.China State Key Laboratory of Rare Earth Resource Utilizations Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022P.R.China

Three-dimensional（3D） flowerlike hierarchical Zn2GeO4 and Mn2＋-doped Zn2GeO4 microstructures have been prepared by a facile hydrothermal approach. X-Ray diffraction（XRD）, field emission scanning electron micro-scopy（FESEM）, transmission electron microscopy（TEM） and photoluminescence（PL） spectrometry were employed to characterize the samples. Such flowerlike hierarchical Zn2GeO4 microstructures with an average diameter of 3―4 μm were found to be constructed by abundant single crystalline nanorods of about 90 nm in diameter. The luminescent properties of Zn2GeO4：xMn phosphors with different contents of Mn2＋ as an activator were investigated. The Mn2＋-doped samples showed green luminescence corresponding to the d-d transition of Mn2＋ under the irradiation of UV light. The red shift（from 531 nm to 538 nm） in λem with increasing Mn2＋ content was observed in the luminescent spectra, which should be attributed to a weak crystal field because of the substitution of Zn2＋ by Mn2＋ at a distorted tetrahedral lattice site.

关键词： Zinc orthogermanate Hierarchical architecture Luminescent property

Direct Activation of CFTR Chloride Channel by Natural Compound Theophylline

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Chemical Research in Chinese Universities 2009年第6期25卷 887-890页

作者： LIU Xin GE Hong ZHAO Xue-liang HOU Ting-ting sun juan-juan YANG Jing-bo HE Cheng-yan YANG Hong China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China School of Life Sciences Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery Liaoning Normal University Dalian 116029 P. R. China School of Chemical Engineering and Technology Tianjin University Tianjin 300072 P. R. China

Theophylline has been widely used in the＇treatment of airway disease but molecular mechanism has not been clearly elucidated. Previous studies have manifested that theophylline increases intracellular cAMP concentra- tion. Because cystic fibrosis transmembrane conductance regulator（CFTR） is a cAMP-dependent Cl- channel that plays key roles in fluid secretion in vivo, we postulated that theophylline activates CFTR channel gating. We found （1） theophylline stimulated CFTR-mediated anion transport in a concentration-dependent manner, and CFTR specific blocker completely reversed the effect; （2） theophylline had no effect on △F508 or G551D mutant CFTR chloride channel activity; （3） theophylline had additive effect with forskolin（FSK） and 3-isobutyl-xanthin（IBMX）, thus a direct binding activation mechanism was suggested. In conclusion, the results may provide a clue to elucidating the molecular mechanism of theophylline activities and theophylline may present a novel lead drug in treating CFTR-related disease.

关键词： Theophylline Cystic fibrosis transmembrane conductance regulator Activator

基于生物刺激的中性地浸采铀地下水修复试验研究

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铀矿冶 2024年第1期43卷 65-70页

作者：安毅夫孙娟高扬连国玺杨冰张昊岩中核第四研究设计工程有限公司河北石家庄050021 生态环境部核与辐射安全中心北京100082 北京师范大学环境学院北京100875

以某中性地浸铀矿山采区内地下水为研究对象,基于生物刺激修复方式,加入碳源,开展了地下水+砂岩混合体系的污染修复室内试验,筛选得到了优势碳源,确定了优势碳源的最佳投加水平,并验证了优势碳源对U的修复效果。结果表明,乙醇对中性地... 详细信息

以某中性地浸铀矿山采区内地下水为研究对象,基于生物刺激修复方式,加入碳源,开展了地下水+砂岩混合体系的污染修复室内试验,筛选得到了优势碳源,确定了优势碳源的最佳投加水平,并验证了优势碳源对U的修复效果。结果表明,乙醇对中性地浸地下水中U的修复效率最高,可达97.48%,修复效果与pH和Eh显著相关(Pearson’s r>0.7,P<0.01)。乙醇的生物代谢过程对水-岩体系的扰动较少,能保证HCO_(3)^(-)和SO_(4)^(2-)等离子环境的相对稳定,更易实现地下水中U的长期稳定。

关键词：中性地浸采铀地下水生物刺激碳源微生物修复

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围产期心肌病反复心血管多发血栓1例

心脏杂志 2024年第1期36卷 121-121,124页

作者：肖丞炜赵雪冰孙立娟河北医科大学超声医学教研室河北石家庄050000 秦皇岛市第一医院超声诊断科河北秦皇岛066000

1临床资料患者,女,30岁,孕2产2,主因“分娩后呼吸困难、下肢浮肿半个月”入院。查体:体温36.4℃,脉率92次/min,呼吸20次/min,血压106/86 mmHg(1 mm Hg=0.133kPa)。神志清,精神差,颈静脉充盈,双肺可闻及湿啰音。心率92次/min,律齐,未闻... 详细信息

1临床资料患者,女,30岁,孕2产2,主因“分娩后呼吸困难、下肢浮肿半个月”入院。查体:体温36.4℃,脉率92次/min,呼吸20次/min,血压106/86 mmHg(1 mm Hg=0.133kPa)。神志清,精神差,颈静脉充盈,双肺可闻及湿啰音。心率92次/min,律齐,未闻及杂音。腹软,无压痛、反跳痛及肌紧张。双下肢无水肿。超声心动图提示:全心增大,左心房及左心室心尖附壁血栓形成,二尖瓣少量反流,三尖瓣中量反流,肺动脉轻度高压,左心功能减低。

关键词：围产期心肌病血栓超声心动图

Effect of NaCI and Helicobacter pylori vacuolating cytotoxin on cytokine expression and viability

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World Journal of Gastroenterology 2006年第14期12卷 2174-2180页

作者： juan sun Kazuo Aoki Jin-Xu Zheng Bing-Zhong Su Xiao-Hui Ouyang Junichi Misumi Department of Public Health and Hygiene(Ⅱ) Faculty of Medicine Oita University Oita 879-5593 Japan Department of Pulmonary Medicine Affiliated Hospital School of Medicine Jiang Su University Zhenjiang 212001China First Affiliated Hospital of Inner Mongolia Medical College Hohhot 010000 Inner Mongolia China

AIM; To determine whether Helicobacter pylori （H pylon） vacuolating cytotoxin （VacA） regulates release of proinflammatory cytokines （IL-1β, IL-8, TNF-α, and IL-6） or alters gastric epithelial cell viability and to determine whether NaCl affects these VacA-induced changes. METHODS： Vacuolating activity was determined by measuring the uptake of neutral red into vacuoles of VacA-treated human gastric epithelial （AGS） cells. AGS cell viability was assessed by direct cell counting. Specific enzyme-linked immunosorbent assays （ELISA） and reverse transcdptase-polymerase chain reaction（RT-PCR） were performed to examine the effects of H pylori VacA and NaCl on cell pro-inflammatory cytokine production in AGS cells. Immunohistochemical staining of gastric tissue from Mongolian gerbils was used to confirm VacAinduced pro-inflammatory cytokine production and the effects of NaCl on this VacA-induced response. RESULTS： Addition of VacA alone reduced AGS cell viability （P〈 0.05）, and this reduction was enhanced by high doses of NaCl （P〈0.05）. VacA alone induced expression of TNF-α, IL-8 and IL-1β, while NaCl alone induced expression of TNF-α and IL-1β. Changes in mRNA levels in the presence of both VacA and NaCl were more complicated. For the case of TNF-α, expression was dosedependent on NaCl. IL-6 mRNA was not detected. However, low levels of IL-6 were detected by EUSA. Positive immunohistochemical staining of IL-1, IL-6, and TNF-α was found in gastric tissue of H pylori-infected gerbils fed with either a normal diet or a high salt diet. However, the staining of these three cytoldnes was sb＇onger in H pylori-infected animals fed with a 5g/kg NaCl diet. CONCLUSION： VacA decreases the viability of AGS cells, and this effect can be enhanced by NaCl. NaCl also affects the production of pro-inflammatory cytokines in- duced by VacA, suggesting that NaCl plays an important role in Hpylori-induced gastric epithelial cell cytotoxicity.

关键词： Helicobacter pylori Vacuolating cytotoxin Cytokine Gerbil AGS cell

基于定量蛋白质组学探讨西黄丸抗乳腺增生的作用机制

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中国药理学通报 2024年第9期40卷 1641-1648页

作者：陶蕊王婧瑞王俊亮马学莉孙娟霞高广淼范琪瑞韩涛甘肃中医药大学中药学教研室甘肃兰州730000 甘肃省中药药理与毒理学重点实验室甘肃兰州730000

目的基于定量蛋白质组学技术明确西黄丸抗乳腺增生(hyperplasia of mammary glands,HMG)中乳腺组织的差异蛋白并验证,探讨其作用机制。方法将SD大鼠随机分为空白组、模型组和西黄丸组,每组10只。除空白组外,肌注雌孕激素建立HMG大鼠模型... 详细信息

目的基于定量蛋白质组学技术明确西黄丸抗乳腺增生(hyperplasia of mammary glands,HMG)中乳腺组织的差异蛋白并验证,探讨其作用机制。方法将SD大鼠随机分为空白组、模型组和西黄丸组,每组10只。除空白组外,肌注雌孕激素建立HMG大鼠模型,为期30 d。给药西黄丸14 d后,观察各组大鼠表观形态变化,HE染色观察乳腺组织病理改变,定量蛋白质组学技术筛选各组差异表达蛋白(differentially expressed proteins,DEPs),并进行生物信息学分析和Western blot验证。结果与模型组比较,西黄丸组表观病理形态明显改善,大鼠乳头高度直径明显降低(P<0.01),组织病理程度明显缓解。定量蛋白质组学鉴定出乳腺组织4299个DEPs,对西黄丸组与空白组相对模型组变化一致的14个DEPs进行生物信息学分析,发现与肌肉系统过程的调节、肌肉收缩调节、DNA复制和DNA复制启动前过程有关。Western blot结果表明,与模型组比较,西黄丸组大鼠乳腺组织ACLY与ALDOC蛋白表达水平明显降低,BIN1蛋白表达水平明显增加(P<0.01)。结论西黄丸可能通过调控BIN1、ACLY及ALDOC蛋白水平,调控DNA复制、DNA复制启动前和肌肉系统过程的调节、肌肉收缩调节等途径发挥抗HMG作用。

关键词：西黄丸蛋白质组学乳腺增生 BIN1蛋白 ACLY蛋白 ALDOC蛋白

Studies on the Structure of Gel during Preparing ZnO Thin Films by Sol-gel Method

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Chinese Journal of Structural Chemistry 2010年第7期29卷 1061-1066页

作者： QIN Xiu-juan sun Xue-Liang BU Li-Min ZUO Hua-Tong HANSI Hui-Zhi College of Environmental and Chemical Engineering State Key Laboratory of Metastable Materials Science and Technology Yanshan University Qinhuangdao 066004 China

Transparent thin films of ZnO have been prepared on ordinary glass substrates by the inorganic sol-gel method using citric acid as chelating agent and zinc nitrate as the starting material. A novel structure on zinc citrate complex was put forward by using DTA-TG and FT-IR absorbanee spectrum of citrate gels. Phase formation, morphology and optical properties of ZnO films are investigated by XRD, AFM and UV-vis transmittance spectra. The experimental results show that ZnO thin films derived from zinc citrate sol-gel method showed a （002） oriented hexagonal wurtzite structure, good crystalline property, a uniform range of grain size （40 nm）, smooth surface of films, band gap of 3.28 eV and optical transmittances ratio over 90% in the visible range.

关键词： ZnO thin film inorganic complex sol-gel infrared spectra zinc citrate complex