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Cell Research 2004年第6期14卷 507-512页

作者： s.s.koide 1230 York Avenue Center for Biomedical Research Population Council NY 10021 New York UsA

The method of laser capture microdissection (LCM) combined with suppressive subtractive hybridization (ssH) was developed to isolate specific germ cells from human testis sections and to identify the genes expressed during differentiation and development. In the present study, over 10,000 primary spermatocytes and round spermatid cells were successfully isolated by LCM. Using the cDNAs from primary spermatocytes and round spermatids, ssH cDNAs library of primary spermatocyte-specific was constructed. The average insert size of the cDNA isolated from 75 randomly picked white clones was 500 bp, ranging from 250 bp to 1.7 kb. Using the dot-blot method, a total of 421 clones were examined, resulting in the identification of 390 positive clones emitting strong signals. Partial sequence of cDNAs prepared from each clone was determined with an overall success rate of 84.4%. Genes encoding cytochrome c oxidase II and the rescue factor-humanin were most frequently expressed in primary spermatocytes, suggesting th

关键词： laser capture microdissection suppressive subtractive hybridization spermatogenesis cytochrome c oxidase Ⅱ humanin.

人精子膜蛋白片段在沙门氏菌中的表达——一种制备抗生育疫苗的途径(英文)

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实验生物学报 1998年第1期31卷 49-60页

作者：高爱武严缘昌杨岐生赵峰董琦张美兰 s.s.koide 中国科学院上海细胞生物学研究所上海200032 浙江大学生物技术与科学系杭州310027 人口委员会生物医学研究中心

合成编码一种人精子膜蛋白YWK-Ⅱ胞外区的一段多肽片段的双链寡核昔酸链,HsD—2a。用平端连接的方法将其插入到沙门氏菌鞭毛基因fliC(d)的抗原表位IV高变区EcoRV位点,构建了重组质粒pLs408-H1。重组基因在鞭毛负性aroA基因缺失的无致病... 详细信息

合成编码一种人精子膜蛋白YWK-Ⅱ胞外区的一段多肽片段的双链寡核昔酸链,HsD—2a。用平端连接的方法将其插入到沙门氏菌鞭毛基因fliC(d)的抗原表位IV高变区EcoRV位点,构建了重组质粒pLs408-H1。重组基因在鞭毛负性aroA基因缺失的无致病性沙门氏菌*** sL5928疫苗菌株中表达。经ELIsA、电镜免疫胶体金法检测,表明HsD-2a编码的多肽片段成功地在沙门氏菌鞭毛表面表达。融合鞭毛蛋白分子量约为60kD。Western-Blot法检测,提纯的融合鞭毛蛋白与YWK-Ⅱ小鼠抗血清只在60 kD处出现一条结合带。携带重组质粒pLs408—H1的沙门氏菌疫苗菌株极有可能成为抗生育疫苗。

关键词：人精子膜蛋白沙门氏菌疫苗菌株避孕疫苗

INHIBITION OF ZYGOTE FORMATION AND EMBRYO DEVELOPMENT IN MICE IMMUNIZED WITH MONOCLONAL ANTIsPERM ANTIBODIEs

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Chinese Medical sciences Journal 1990年第1期8卷 9-12页

作者：严缘昌梁平王琳芳缪时英 samuel s.koide shanghai Institute of cell Biology Academia SinicaShanghai Institute of Basic Medical sciences CAMSBeijing Population Council Center for Biomedical Research New York.

Passive immunization of female mice by the intraperitoneal administration of ascitic fluidscontaining monoclonal antisperm antibodies blocked the development of mouse zygotes tomorulae and blastocysts when cultured in *** YWK-I monoclonal anti-bodies were prepared and administered intraperitoneally to Jemale *** radioac-tivity was found in washes of the oviductal *** results suggest that antispermantibodies block fertilization and may have a deleterious effect on early developing embryos.

关键词： antisperm antibodies embryo development

CLONING AND IDENTIFICATION OF A RABBIT sPERM MEMBRANE PROTEIN GENE

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Chinese Medical sciences Journal 1990年第1期8卷 4-8页

作者：王琳芳缪时英严缘昌王维刘强远白云朱红 s.koide Chen Chinling C.W.Bardin Institute of Basic Medical sciences CAMSBeijing shanghai Institute of Cell Biology Shanghai Center for Biomedical Research The Population Council New York.

Antibodies raised against rabbit sperm membrane protein(rsMP-B)interacted with a rattestis cytosolic protein with an estimated molecular weight of 70 *** clone expressingrsMP-B was isolated by epitope selection from a rat testis,λgt11 vector cDNA library,An Eco Rl-eut 2.0 kb cDNA fragment encoding rsMP-B was *** cDNA fragment(RsD-1)was isolated,subcloned and restriction *** hybridization analysis of mRNAprepared from human,rabbit and rat testes showed that the gene encoding rsMP-B is ex-pressed in the testes of all three mammals.

关键词： cDNA sperm membrane protein

Fabrication and structural characterization of TiO nanoparticles soft-landed on substrate by the combined sputtering and gas aggregation method

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Fabrication and structural characterization of TiO nanoparti...

Atmospheric pollution in Beijing, China, as recorded in sediments of the Kunming Lake

Ⅻ International symposium on small Particles and Inorganic Clusters

作者： s.Tanemura Y.Kajino s.koide L.Miao M.Tanemura s.Toh K.Kaneko M.Mori Department of Environmental Technology ＆ Urban Planning Nagoya Institute of TechnologyShowa-kuNagoya466-8555Japan Department of Environmental Technology ＆ Urban Planning Nagoya Institute of TechnologyShowa-kuNagoya466-8555Japan Department of Environmental Technology ＆ Urban Planning Nagoya Institute of TechnologyShowa-kuNagoya466-8555Japan Department of Environmental Technology ＆ Urban Planning Nagoya Institute of TechnologyShowa-kuNagoya466-8555Japan Department of Environmental Technology ＆ Urban Planning Nagoya Institute of TechnologyShowa-kuNagoya466-8555Japan Department of Material science and Engineering ＆ HVEM Lab. Kyushu UniversityHigasi-kuFukuoka812-8581Japan Department of Material science and Engineering ＆ HVEM Lab. Kyushu UniversityHigasi-kuFukuoka812-8581Japan Materials Research Laboratory NGK Insulators Ltd. Mizuho-ku Nagoya 467-8530 Japan

＜正＞ Among transition metal oxides, Ti-oxide systems such as TiO2 （titania） and TiO have attracted considerable interests for photo-catalytic and wide band gap characters in titania, and for low electric conductivity in TiO which is preferable characteristics to apply as capacitor in integrated micro-electronic device. Hence, we desire to fabricate nano-particles to control band gaps of Ti-oxide system to adapt to the application criteria.

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Journal of Environmental sciences 1991年第1期3卷 109-116页

作者： Kathe K.Bertines Minoru koide Edward D.Goldberg san Diego state University San Diego 92182 California USA.Scripps Institution of Oceanography La Jolla 92093 California USA.Scripps Institution of Oceanography La Jolla 92093 California USA.

The fluxes of heavy metals and magnetite, a fly-ash component, from coal burning, to sediments of the Kunming Lake in Beijing have increased measurably over the last fifty years. Ages were introduced to the sediment strata through Pb-210 geochronologies. Even with a doubling of coal usage by the year 2000, the anthropogenic metal fluxes will still be less than those recorded in metropolitan Tokyo and U.s. lakes.

关键词： atmospheric pollution sediment heavy metal geochronology.

schottky photodiode using submicron thick diamond epilayer for flame sensing

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Nano-Micro Letters 2009年第1期1卷 30-33页

作者： Y.koide M.Y.Liao J.Alvarez M.Imura K.sueishi F.Yoshifusa sensor Materials Center (senMC) National Institute for Materials Science (NIMS) Laboratoire de Génie Electrique de Paris (UMR 8507 CNRs) Ecole Supérieure d’Electricité Universités Paris VI et Paris XI International Center for Materials Nanoarchitectonics (MANA) National Institute for Materials Science (NIMS) ANTEC Co.

The sensing of a flame can be performed by using wide-bandgap semiconductors, which offer a high signal-to-noise ratio since they only response the ultraviolet emission in the flame. Diamond is a robust semiconductor with a wide-bandgap of 5.5 e V, exhibiting an intrinsic solar-blindness for deep-ultraviolet(DUV) detection. In this work, by using a submicron thick boron-doped diamond epilayer grown on a type-Ib diamond substrate, a schottky photodiode device structure- based flame sensor is demonstrated. The photodiode exhibits extremely low dark current in both forward and reverse modes due to the holes depletion in the epilayer. The photodiode has a photoconductivity gain larger than 100 and a threshold wavelength of 330 nm in the forward bias mode. CO and OH emission bands with wavelengths shorter than 330 nm in a flame light are detected at a forward voltage of-10 V. An alcohol lamp flame in the distance of 250 mm is directly detected without a focusing lens of flame light.

关键词： Flame sensing Boron-doped diamond schottky photodiode

Characterization of rtsHsp13 gene encoding a development protein involved in vesicular traffic in spermiogenesis

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Cell Research 2004年第3期 197-207页

作者： samuel s. koide Center for Biomedical Research Population Council 1230 York Avenue New York NY 10021 UsA

A cDNA, designated as rtsH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids, which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439. The deduced rtsH3p13 protein is a truncated isoform of sH3p13 as a result of mRNA alternative splicing. It is mainly expressed in the rat testis, detected in spermatids at the steps 8-19 of spermiogenesis, and found around the acrosome. During postnatal development, rtsH3p13 appears on day 18 and reaches maximum on day 60. Further experimental results suggested that rtsH3p13 forms a complex with activated epidermal growth factor receptor （EGFR） and interacts with synaptojanin I. surprisingly, similar to sH3 domain, the V region of rtsH3p13 also inhibits endocytosis in CHO cells. Our results reveal a link between an rtsH3p13-synaptojanin-clathrin complex-mediated formation of pits and the process of spermiogenesis.

关键词： rtsH3p13 spermiogenesis acrosome clathrin endocytosis.

Helicobacter heilmannii sensu stricto-related gastric ulcers:A case report

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World Journal of Gastroenterology 2014年第12期20卷 3376-3382页

作者： Takehisa Matsumoto Masatomo Kawakubo Taiji Akamatsu Naohiko koide Naoko Ogiwara seiko Kubota Mitsutoshi sugano Yoshiyuki Kawakami Tsutomu Katsuyama Hiroyoshi Ota Departments of Laboratory Medicine Shinshu University Hospital Endoscopy Center Suzaka HospitalNagano Prefectural Hospital Organization Department of surgery Nagano Prefectural Kiso Hospital Department of Health and Medical sciences Shinshu University Graduate School of Medicine Maruko Central Hospital

A spiral bacterium (sH9), morphologically different from Helicobacter pylori (H. pylori), was found in a 62-year-old woman’s gastric mucosa. Gastroscopic examination revealed multiple gastric ulcers near the pyloric ring; mapping gastric biopsy showed mild mononuclear infiltration with large lymphoid follicles in the antrum, without corpus atrophy. Urea breath test and H. pylori culture were negative, but Giemsa staining of biopsies revealed tightly coiled bacteria that immunostained with anti-H. pylori antibody. sequencing of sH9 16s rRNA and the partial urease A and B subunit genes showed that the former sequence had highest similarity (99%; 1302/1315 bp) to Helicobacter heilmannii (H. heilmannii) sensu stricto (H. heilmannii s.s.) BC1 obtained from a bobcat, while the latter sequence confirmed highest similarity (98.3%; 1467/1493 bp) to H. heilmannii s.s. HU2 obtained from a human. The patient was diagnosed with multiple gastric ulcers associated with H. heilmannii s.s. infection. After triple therapy (amoxicillin, clarithromycin, and lansoprazole) with regimen for eradicating H. pylori, gastroscopy showed ulcer improvement and no H. heilmannii s.s. upon biopsy.

关键词： Helicobacter heilmannii sensu stricto, “ Helicobacter heilmannii” type 2, Gastric ulcers, Urease gene, Non-Helicobacter pylori helicobacters

Comparison of Public Health Nurse’s Lifestyle Counseling skills for Clients with Different Readiness of Behavior Changes in Primary Care settings

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Health 2017年第6期9卷 921-929页

作者： Keiko koide Reiko Okamoto Mari Okada Faculty of Education Shitennoji University Osaka Japan Division of Health sciences Graduate School of Medicine Osaka University Osaka Japan Faculty of Health and Welfare Prefectural University of Hiroshima Hiroshima Japan

Purpose: Public health nurses (PHNs) are required to assess the readiness of the clients and provide lifestyle counseling accordingly. The purpose of this study was to compare the lifestyle counseling provided for clients with different levels of readiness based on self-evaluations and independent assessment. Methods: Participants were PHNs with 10 years’ experience or less. Lifestyle counseling skills were measured through a simulated counseling session in a primary setting lasting up to 30 minutes, followed by a review session, where the participant provided self-evaluations, and the simulated client provided others-evaluations, of the session. The simulated clients played the role of clients at either the pre-contemplation or contemplation stage of preparedness as per the stages of behavior change theory. Results: The self-evaluation results showed that the mean scores for five of the six skill categories, are lower in the precontemplation-stage client cases than in the contemplation-stage client cases, and significantly so for two skill categories. The others-evaluations showed significantly lower mean scores for all skill categories in the precontemplation cases than in the contemplation cases. Conclusion: The PHNs and simulated clients agreed that lifestyle counseling skills were inadequate for the precontemplation cases, as compared with the contemplation cases. The lifestyle counseling skills of PHNs with less experience may not be well-adjusted to the readiness of the client, indicating difficulties in supporting less prepared clients.

关键词： skills in Lifestyle Counseling Readiness of Behavior Change Public Health Nursing Lifestyle-Related Disease