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Biomimetic Phosphohydrolase Nanozyme Based on Defect-Engineered Metal-Organic Framework

CCs Chemistry 2024年第7期6卷 1821-1835页

作者： Xiaoxue kou Yuhong Lin Yong shen Linjing Tong Rui Gao suya Liu siming Huang Fang Zhu Guosheng Chen Gangfeng Ouyang MOE Key Laboratory of Bioinorganic and synthetic Chemistry School of ChemistrySun Yat-sen UniversityGuangzhou 510006 shanghai NanoPort Thermo Fisher ScientificPudong DistrictShanghai 200120 Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target and Clinical Pharmacology The NMPA and State Key Laboratory of Respiratory DiseaseSchool of Pharmaceutical Sciences and The Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436

Phosphodiester bonds are the backbone linkages of all the nucleic acids which store and transfer biological *** hydrolysis of phosphodiester bonds is of great importance to the replication,recombination,and damage repair of DNA as well as the maturation and processing of ***,the spontaneous scission of individual phosphodiester linkages is kinetically challenging under physiological conditions,with an estimated half-life of 30 million ***,we discover a defect-engineered Zr-metal-organic framework(Zr-MOF)nanozyme containing cluster-missing reo defects,named UiO-66 REO,that possesses intrinsic phosphodiesterase-like activity that allows for the cleavage of the phosphodiester bond in physiological condition(pH=7.4 and 37℃),outperforming the activityrecording *** atomic-level structure of the UiO-66 REO nanozyme has been directly identified by the synchrotron radiation absorption spectrum and integrated differential phase contrast-scanning transmission electron *** find that both the defective boundary regions and unusual Zr6-clusters formed in the reo phase of the UiO-66 REO nanozyme contribute to the improvement in efficiency of phosphodiesterase-like hydrolysis,which realizes the cleavage of DNA in mild *** work offers a new insight into biomimetic enzymology using crystal and defect engineering and opens up new possibilities for the development of low-cost,structurally stable and sustainable phosphodiesterase nanozymes for different biological applications.

关键词： nanozyme metal-organic framework biomimetic catalysis hydrolase-like activity defective engineering

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An integrated microfluidic platform for nucleic acid testing

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Microsystems & Nanoengineering 2024年第3期10卷 181-194页

作者： Antao sun Petra Vopařilová Xiaocheng Liu Bingqian kou TomášŘezníček TomášLednický sheng Ni JiříKudr Ondřej Zítka Zdenka Fohlerová Petr Pajer Haoqing Zhang Pavel Neužil Ministry of Education Key Laboratory of Micro and Nano systems for Aerospace School of Mechanical EngineeringNorthwestern Polytechnical University127 West Youyi RoadXi’anShaanxi 710072P.R.China Department of Chemistry and Biochemistry Mendel University in BrnoZemědělská161300 BrnoCzech Republic ITD Tech s.r.o Osvoboditelů100573581 BohumínCzech Republic Central European Institute of Technology Brno University of TechnologyPurkyňova 123Brno 61200Czech Republic Department of Electronic and Computer Engineering The Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong SARChina Department of Microelectronics Faculty of Electrical Engineering and CommunicationBrno University of TechnologyTechnická3058/10Brno 61600Czech Republic Military Health Institute U Vojenskénemocnice 120016200 Praha 6Czech Republic The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and TechnologyXi’an Jiaotong UniversityXi’anShaanxi 710049P.R.China Bioinspired Engineering and Biomechanics Center(BEBC) Xi’an Jiaotong UniversityXi’an 710049P.R.China

This study presents a rapid and versatile low-cost sample-to-answer system for sARs-CoV-2 *** system integrates the extraction and purification of nucleic acids,followed by amplification via either reverse transcriptionquantitative polymerase chain reaction(RT–qPCR)or reverse transcription loop-mediated isothermal amplification(RT–LAMP).By meeting diverse diagnostic and reagent needs,the platform yields testing results that closely align with those of commercial RT-LAMP and RT‒qPCR *** advantages of our system include its speed and *** assay is completed within 28 min,including sample loading(5 min),ribonucleic acid(RNA)extraction(3 min),and RT-LAMP(20 min).The cost of each assay is≈$9.5,and this pricing is competitive against that of Food and Drug Administration(FDA)-approved commercial *** some RNA loss during on-chip extraction is observed,the platform maintains a potential limit of detection lower than 297 *** makes the system particularly useful in environments where centralized laboratories are either unavailable or inconveniently *** key feature is the platform’s versatility,allowing users to choose between RT‒qPCR or RT‒LAMP tests based on specific requirements.

关键词： testing maintain versatile

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Neglected Time Intervals Before “1-3-7” Approach About Imported Malaria Cases-China, 2014–2021

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China CDC weekly 2024年第17期6卷 363-367页

作者： Zhiquan He Yuanjing kou Dan Wang Ying Liu Xiaofeng Lyu Hui Yan Wen Lin Xuan Zhang Duoquan Wang shizhu Li Hongwei Zhang Henan Provincial Center for Disease Control and Prevention Zhengzhou CityHenan ProvinceChina Anhui Provincial Center for Disease Control and Prevention Hefei CityAnhui ProvinceChina Guangxi Zhuang autonomous Region Center for Disease Control and Prevention Nanning CityGuangxi Zhuang Autonomous RegionChina Hubei Provincial Center for Disease Control and Prevention Wuhan CityHubei ProvinceChina Zhejiang Provincial Center for Disease Control and Prevention Hangzhou CityZhejiang ProvinceChina National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)National Health Commission Key Laboratory of Parasite and Vector BiologyShanghaiChina school of Global Health Chinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghaiChina

What is already known about this topic?China’s“1-3-7”approach outlines specific targets to guide and monitor the processes of case reporting,investigation,and ***,few studies have examined the time intervals preceding the initial step,and the timeline from the arrival of imported malaria cases in China to their diagnosis has been largely *** is added by this report?The study demonstrated that the median duration from arrival in China to the onset of symptoms for *** was 78 days,with 71.59%of imported cases manifesting symptoms after more than one *** ***,the median interval was 42 days,with 55.91%exceeding one ***,the median time from symptom onset to malaria treatment in China between 2014 and 2021 was 2 days,with an interquartile range(IQR)of 1-4 *** are the implications for public health practice?This study represents the initial effort to delineate the chronology of imported malaria cases,from their arrival in China to their subsequent *** results underscore the importance of providing malaria health education to populations arriving from ***,enhancing physician training is crucial for improving the diagnosis of malaria.

关键词： diagnosis Malaria exceeding

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马克思剩余劳动概念的伦理意蕴

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理论视野 2024年第6期292卷 5-10页

作者：寇东亮陕西师范大学哲学学院

剩余劳动概念在马克思剩余价值理论的创建和唯物史观的完善中具有至关重要的意义,它关涉剩余价值源泉、剥削秘密、脑体分工、物质财富、自由时间、人的全面发展等主题,具有丰富的伦理意蕴。这主要表现在三个方面:一是通过“劳动力商品... 详细信息

剩余劳动概念在马克思剩余价值理论的创建和唯物史观的完善中具有至关重要的意义,它关涉剩余价值源泉、剥削秘密、脑体分工、物质财富、自由时间、人的全面发展等主题,具有丰富的伦理意蕴。这主要表现在三个方面:一是通过“劳动力商品”批判和“剩余劳动时间”分析,凸显剩余劳动概念的生命伦理意蕴;二是通过“脑体劳动分工”批判和“剩余劳动产品”分析,凸显剩余劳动概念的劳动伦理意蕴;三是通过“为生产而生产”批判和“剩余劳动积累”分析,凸显剩余劳动概念的财富伦理意蕴。

关键词：马克思剩余劳动生命财富伦理

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Multimodal imaging in the diagnosis of bone giant cell tumors:A retrospective study

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World Journal of Clinical Cases 2024年第16期12卷 2722-2728页

作者： Ming-Qing kou Bing-Qiang Xu Hui-Tong Liu Department of Medical Imaging Shaanxi Provincial People's HospitalXi'an 710068Shaanxi ProvinceChina Department of Orthopedics Shaanxi Provincial People's HospitalXi'an 710068Shaanxi ProvinceChina

BACKGROUND Giant cell tumor of bone is a locally aggressive and rarely metastasizing tumor,and also a potential malignant tumor that may develop into a primary malignant giant cell *** To evaluate the role of multimodal imaging in the diagnosis of giant cell tumors of *** The data of 32 patients with giant cell tumor of bone confirmed by core-needle biopsy or surgical pathology at our hospital between March 2018 and March 2023 were retrospectively *** the patients with giant cell tumors of the bone were examined by X-ray,computed tomography(CT)and magnetic resonance imaging(MRI),and 7 of them were examined by positron emission tomography(PET)-*** X-ray imaging can provide overall information on giant cell tumor *** and MRI can reveal the characteristics of the internal structure of the tumor as well as the adjacent relationships of the tumor,and these methods have unique advantages for diagnosing tumors and determining the scope of ***-CT can detect small lesions and is highly valuable for identifying benign and malignant tumors to aid in the early diagnosis of *** Multimodal imaging plays an important role in the diagnosis of giant cell tumor of bone and can provide a reference for the treatment of giant cell tumors.

关键词： Giant cell tumor of bone Multimodal imaging Computed tomography Magnetic resonance imaging Positron emission tomography-computed tomography

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Loss of Tet hydroxymethylase activity causes mouse embryonic stem cell differentiation bias and developmental defects

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science China(Life sciences) 2024年第10期67卷 2132-2148页

作者： Mengting Wang Liping Wang Yanxin Huang Zhibin Qiao shanru Yi Weina Zhang Jing Wang Guang Yang Xinyu Cui Xiaochen kou Yanhong Zhao Hong Wang Cizhong Jiang shaorong Gao Jiayu Chen Clinical and Translational Research Center of shanghai First Maternity and Infant Hospital Shanghai Key Laboratory of Signaling and Disease ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China shanghai Tenth People’s Hospital School of MedicineTongji UniversityShanghai 200072China Frontier science Center for stem Cell Research Tongji UniversityShanghai 200092China Key Laboratory of spine and spinal Cord Injury Repair and Regeneration of the Ministry of Education Orthopaedic Department of Tongji HospitalTongji UniversityShanghai 200065China shanghai Institute of stem Cell Research and Clinical Translation Shanghai 200120China

The TET family is well known for active DNA demethylation and plays important roles in regulating transcription,the epigenome and ***,previous studies using knockdown(KD)or knockout(KO)models to investigate the function of TET have faced challenges in distinguishing its enzymatic and nonenzymatic roles,as well as compensatory effects among TET family members,which has made the understanding of the enzymatic role of TET not accurate *** solve this problem,we successfully generated mice catalytically inactive for specific Tet members(Tetm/m).We observed that,compared with the reported KO mice,mutant mice exhibited distinct developmental defects,including growth retardation,sex imbalance,infertility,and perinatal ***,Tetm/mmouse embryonic stem cells(mEsCs)were successfully established but entered an impaired developmental program,demonstrating extended pluripotency and defects in ectodermal differentiation caused by abnormal DNA ***,Tet3,traditionally considered less critical for m EsCs due to its lower expression level,had a significant impact on the global hydroxymethylation,gene expression,and differentiation potential of ***,there were common regulatory regions between Tet1 and Tet3 in pluripotency *** summary,our study provides a more accurate reference for the functional mechanism of Tet hydroxymethylase activity in mouse development and EsC pluripotency regulation.

关键词： Tet hydroxymethylase activity mEsCs DNA demethylation 5hmC

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Primary Total Hip Arthroplasty on Complex Hips Conditions in a Low-Resource setting

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Open Journal of Orthopedics 2024年第7期14卷 325-333页

作者： souleymane Ouédraogo Malick Diallo s.kou sidibé Massadiami soulama Adama sidibé Ousmane Ouermi Patrick W. H. Dakouré Orthopedics and Traumatology Department University Hospital Sour SANOU Bobo-Dioulasso Burkina Faso superior Institute of Medical sciences University Nazi BONI Bobo-Dioulasso Burkina Faso

Introduction: Total hip arthroplasty of complex morphology is a challenge for the orthopaedic surgeon. Careful analysis of the hip’s anatomy helps to unravel the difficulties and anticipate the procedures to be performed and the implants to be planned. The aim was to identify the types of hip that make first-line THR difficult, specify the technical procedures to be used on these sites, and assess the functional results of the series. Material and Methods: This was a retrospective study that concerned patients operated on for total hip arthroplasty between January 2015 and December 2022 at the medical center “La Grâce” in Bobo-Dioulasso, Burkina Faso. Patients with coxarthrosis (on hip dysplasia, acetabular protrusio, acetabular malunion or neurological hip) and those with ankylosis of the hip, osteonecrosis secondary to neglected dislocation of the hip or hemoglobinopathy were included. Results: A total of 31 total hip replacements were performed in 30 patients. The mean age of patients at the surgery time was 36.2 years with extremes of 17 and 61 years. The male-to-female sex ratio was 1. The main indications for THA were the dysplasic hip osteoarthritis (11 cases) and the neglected hip dislocations (7 cases). In situ femoral neck osteotomy before hip dislocation was performed in seven cases. The acetabulum reconstruction techniques varied from the structural iliac bone graft (n = 3) and cancellous bone graft (n = 4) to the Kerboull plate (n = 1). After 45 months of mean follow-up, all hips were evaluated. The mean PMA score increased from 7.1 [4 - 8] before the surgery to 13.2 [13 - 17]. Conclusion: The large spectrum of challenges in complex hip management requires effective preoperative planning. Preoperative planning minimizes complications and ensures a better outcome.

关键词： Primary Total Hip Arthroplasty Hip Dysplasia Complex Hip

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Impact of Vascular Variations on Living Donor Kidney Transplantation

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Open Journal of Organ Transplant surgery 2024年第1期12卷 1-6页

作者： Noël Coulibaly Evrard kouamé Yao Vamoussa Diomandé Tawakaltu Bolasade Adebayo Légnima s.kou Michel Tuo Donafologo Daouda Yéo UFR sciences Mdicales dAbidjan-Dpartement de spcialits et spcialits Chirurgicales Universit Flix Houphout Boigny Abidjan Cte dIvoire service dUrologie CHU Treichville Abidjan Cte dIvoire

Background: Kidney transplantation is the best treatment for end-stage chronic kidney disease. However, its realization is confronted by several difficulties among which are anatomical variations. Objective: The objective of our work was to describe the impact of renal pedicle variations on the operative procedure as well as the complications. Method: We conducted a retrospective study on living kidney donors and their recipients in the period from 2012 to 2017. several variables were studied, in particular socio-demographic, operative and progression. Results: We identified 49 living donors whose mean age was 37.59 years with a male-to-female ratio of 3.9 and 45 ± 10.75 years in the recipients. The prevalence of renal vascular abnormalities was higher with a proportion of 46.94% in recipients. The left kidney was most frequently removed (75.51%) and then kept mainly in HTK (95.92%). These vascular anomalies were associated with a longer operating time but this difference was not statistically significant (p = 0.5804). They had no effect on hot and cold ischemia times (p = 0.9838, p = 0.8389). Complications were observed in 11 patients, all recipients, i.e. 11.22%, and were not related to the presence of vascular abnormalities (p = 0.086). We observed that 4.08% of deaths were all recipients. Conclusion: It seems that kidney transplantation with multiple renal arteries and/or veins does not significantly lengthen the operating time and does not promote the onset of complications.

关键词： Vascular Abnormality Renal Transplantation Renal Pedicle

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Traumatic Neglected Antero-Medial Knee Dislocation: Case Report

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Open Journal of Orthopedics 2024年第9期14卷 421-426页

作者： souleymane Ouédraogo Massadiami soulama Abdourahmane Ouangré Ousmane Ouermi s.kou sidibé Maik Telyann Tchitembo Malick Diallo Patrick Wendpouiré Hamed Dakouré Orthopedics and Traumatology Department University Hospital Sour Sanou Bobo-Dioulasso Burkina Faso superior Institute of Medical sciences University Nazi Boni Bobo-Dioulasso Burkina Faso surgery Department Regional University Hospital Ouahigouya Burkina Faso Faculty of Medicine University Ledea Bernard Oudraogo Ouahigouya Burkina Faso

Introduction: Neglected knee dislocation is an unusual injury, only a few cases have been reported in the literature. The circumstances of onset are generally an unnoticed knee dislocation in a polytrauma context or a delay in consultation after the initial trauma. Presentation of case: We report the case of a 48-year-old female patient who sustained a knee injury during a domestic accident. Initial treatment was traditional and was followed for 3 years. she then consulted hospital, where a neglected knee dislocation was identified and treated with femorotibial arthrodesis. Discussion and conclusion: The treatment options of neglected knee dislocation include open reduction and fixation, arthrodesis and total knee arthroplasty. That the choice depends on the old unreduced of dislocation.

关键词： Neglected Knee Dislocation Tibiofemoral Arthrodesis Total Knee Arthroplasty

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论不动产抵押合同的特定财产债权担保效果——一种融合人保与物保的非典型担保

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法学家 2024年第3期 160-173,195,196页

作者：寇枫阳吉林大学法学院

《民法典担保制度解释》第46条看似采纳了违约责任的通说,实与担保责任杂糅。违约责任说着眼于抵押登记的单一效果,不仅导致意思表示解释方法失当,且忽视了其理论构造对担保责任的吸收。不动产抵押合同包含担保合意与登记合意,分别产生... 详细信息

《民法典担保制度解释》第46条看似采纳了违约责任的通说,实与担保责任杂糅。违约责任说着眼于抵押登记的单一效果,不仅导致意思表示解释方法失当,且忽视了其理论构造对担保责任的吸收。不动产抵押合同包含担保合意与登记合意,分别产生担保责任与抵押登记请求权,前者具有的以特定财产担保债务履行之效果,才是其担保功能所在,随之带来了违约责任说所欠缺的效率优势。此种担保效果本质上系债权担保,但责任财产限于特定不动产,非典型保证说混淆了人保与物保的基本界限,因而应定性为“特定财产+债权担保”之非典型担保。作为类型融合合同的不动产抵押合同,经《民法典》第467条第1款的准用,得分别参照适用保证合同与生效创设担保物权合同的规范,从而产生“变价权+受偿权”的效果。

关键词：不动产抵押合同抵押登记担保功能非典型担保参照适用

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