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Engineering porous organic polymers for carbon dioxide capture

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science China Chemistry 2017年第8期60卷 1007-1014页

作者： Ning Huang Gregory Day Xinyu Yang Hannah drake Hong-Cai Zhou Department of Chemistry Texas A＆M University College Station TX 77843 US

As atmospheric CO_2 levels rise, the development of physical or chemical adsorbents for CO_2 capture and separation is of great importance on the way towards a sustainable low-carbon future. Porous organic polymers are promising candidates for CO_2 capture materials owing to their structural flexibility, high surface area, and high stability. In this review, we highlight high-performance porous organic polymers for CO_2 capture and summarize the strategies to enhance CO_2 uptake and selectivity, such as increasing surface area, increasing interaction between porous organic polymers and CO_2, and pore surface functionalization.

关键词： porous organic polymers C02 capture nanomaterials post-synthesis polymer

Feed particle selection and nutrient intake altered by pecking stone consumption and beak length in free-range laying hens

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Animal Nutrition 2019年第2期5卷 140-147页

作者： Zafar Iqbal Kelly drake Robert A.swick Rider A.Perez-Maldonado Isabelle Ruhnke school of Environmental and Rural science Faculty of ScienceAgricultureBusiness and LawUniversity of New EnglandArmidaleNSW2351Australia south Australian Research and Development Institute Roseworthy CampusUniversity of AdelaideSouth Australia5371Australia DsM Nutritional Products Singapore117440Singapore

The present study investigated the effects of pecking stones on feeding behaviour of hens from 16 to 46 weeks of age. Eighteen flocks of Hy-Line Brown hens were housed in 2 commercial free-range housing systems. Farm A housed 10 flocks of beak trimmed(infrared beak treatment) hens in fixed sheds. Farm B housed 8 flocks of hens with intact beaks in mobile sheds. On each farm, flocks were equally assigned to control groups(no access to pecking stones) and treatment groups(access to pecking stones). Data were evaluated every 10 weeks. At each time point, 10 hens per flock were housed in individual pens, and each hen was provided with 250 g of mash diet and ad libitum water for 24 h. After 24 h, feed samples were collected and used to determine 24-h feed intake. Nutrient and particle selection was measured by subtracting nutrients and particles present in the leftover feed from the vaules obtained in the offered feed and expressed the change(A). In addition, pecking stone consumption was recorded for each *** were analysed separately for each farm using fixed effects of pecking stone availability and hen ***’s rho correlation coefficients and linear regression models were constructed to evaluate the relationship of beak length and pecking stone usage, discrete mean particle size(dMEAN) consumption(△ dMEAN), and △ nutrient intake. Hens with access to pecking stones consumed significantly lower quantities of large feed particles(>2.8 mm) on farm A(P = 0.029) and selected significantly more fine particles, on farm B(P = 0.013). Overall, positive relationships(P = 0.001) between beak length and pecking stone consumption, A dMEAN, and A phosphorus consumption were observed. In conclusion,pecking stone consumption resulted in reduced selection and consumption of feed particles in hens housed on both farms. Further research is warranted to investigate the effect of pecking stones on sensory innervation of the beak.

关键词： Poultry Welfare Feeding behaviour Nutrition Environmental enrichment

simultaneous biodegradation of nitrogen-containing aromatic compounds in a sequencing batch bioreactor

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Journal of Environmental sciences 2007年第5期19卷 530-535页

作者： LIU Xing-yu WANG Bao-jun JIANG Cheng-ying ZHAO Ke-xin Harold L. drake LIU shuang-Jiang state Key Lab of Microbial Resources at Institute of Microbiology Chinese Academy of Sciences Beijing 100080 China Graduate University of Chinese Academy of sciences Beijing 100049 China Department of Ecological Microbiology University of Bayreuth 95445 Bayreuth Germany

Many nitrogen-containing aromatic compounds （NACs）, such as nitrobenzene （NB）, 4-nitrophenol （4-NP）, aniline （AN）, and 2,4-dinitrophenol （2,4-DNP）, are environmentally hazardous, and their removal from contaminated water is one of the main challenges facing wastewater treatment plants. In this study, synthetic wastewater containing NB, 4-NP, 2,4-DNP, and AN at concentrations ranging from 50 to 180 mg/L was fed into a sequencing batch reactor （sBR）. Analyses of the sBR system indicated that it simultaneously removed more than 99% of the NACs at loading rates of 0.36 kg NB/（m^3·d）, 0.3 kg 4-NP/（m^3·d）, 0.25 kg AN/（m^3·d）, and 0.1 kg 2,4-DNP/（m^3·d）. Bacterial groups of Bacteriodetes, Candidate division TM7, α-Proteobacteria, and β-Proteobacteria were dominant in the clone libraries of 16s rRNA genes retrieved from the microbial communities in the sBR system. ＂Cycle tests＂ designed to alter feeding and aeration parameters of the sBR system demonstrated that the resident microbial biome of the sBR system responded rapidly to changing conditions. Consumption of O2 was concomitant with the apparent mineralization of NACs. Aromatic ring-cleaving dioxygenase activities suggested that （1） AN and NB were degraded via catechol 2,3-dioxygenase; （2） 4-NP was degraded via 1,2,4-benzentriol 1,2-dioxygenase; and （3） 2,4-DNP was degraded via an unresolved pathway.

关键词： nitrogen-containing aromatic compounds biodegradation sequencing batch reactor Candidate division TM7

Isotopic niche provides an insight into the ecology of a symbiont during its geographic expansion

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Current Zoology 2022年第2期68卷 185-197页

作者： Enrique GONZALEZ-ORTEGON Marta PEREZ-MIGUEL Jose I.NAVAs Pilar drake Jose A.CUEsTA Instituto de Ciencias Marinas de Andalucia(ICMAN-CsIC) Campus Universitario Rio San PedroAvda.Republica Saharaui211519CadizPuerto RealSpain Unidad Asociada Crecimiento Azul CsIC-IFAPA El Puerto de Santa Maria SpainSpain Instituto de Investigacion y Formacion Agraria y Pesquera IFAPA-Centro Agua del PinoCtra.EI Rompido-Punta Umbriakm 3.821459 EI RompidoHuelvaSpain

The study of the recent colonization of a symbiont and its interaction with host communities in new locations is an opportunity to understand how they *** use of isotopic ratios in trophic ecology can provide measurements of a species'isotopic niche,as well as knowledge about how the isotopic niches between symbiont and host species *** isotope measurements were used to assess the sources of carbon assimilated by the host species(the bivalves Mytilus galloprovincialis and scrobicularia plana)and their associated symbiont pea crab Afropinnotheres monodi,which occurs within these bivalves'mantle *** mixing model estimates suggest that all of them assimilate carbon from similar sources,particularly from pseudofaeces and particulate organic matter in this symbiotic system based on filter *** symbiotic species occupy comparable trophic levels and its association seems to be commensal or parasitic depending on the duration of such *** pea crab *** reflects a sex-specific diet,where males are more generalist than the soft females because the latter's habitat is restricted to the host *** high isotopic overlap between soft females and *** may reflect a good commensal relationship with the host.

关键词： bivalves commensal kleptoparasitism parasitism stable isotope trophic position

Bioreactor design and validation for manufacturing strategies in tissue engineering

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Bio-Design and Manufacturing 2022年第1期5卷 43-63页

作者： Diana Lim Eric s.Renteria.drake s.sime Young Min Ju Ji Hyun Kim Tracy Criswell Thomas D.shupe Anthony Atala Frank C.Marini Metin N.Gurcan shay soker Joshua Hunsberger James J.Yoo Wake Forest Institute for Regenerative Medicine Wake Forest School of MedicineWinston-SalemNC 27157USA Center for Biomedical Informatics Wake Forest School of MedicineWinston-SalemNC 27157USA RegenMed Development Organization(ReMDO) Winston SalemNC 27106USA

The fields of regenerative medicine and tissue engineering offer new therapeutic options to restore,maintain or improve tissue function following disease or *** maximize the biological function of a tissue-engineered clinical product,specific conditions must be maintained within a bioreactor to allow the maturation of the product in preparation for ***,the bioreactor should be designed to mimic the mechanical,electrochemical and biochemical environment that the product will be exposed to in ***-time monitoring of the functional capacity of tissue-engineered products during manufacturing is a critical component of the quality management *** present review provides a brief overview of bioreactor engineering *** addition,strategies for bioreactor automation,in-line product monitoring and quality assurance are discussed.

关键词： Bioreactor Monitoring sensors and automation Manufacturing Tissue engineering Regenerative medicine

strategies for PET imaging of the receptor for advanced glycation endproducts(RAGE)

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Journal of Pharmaceutical Analysis 2020年第5期10卷 452-465页

作者： Lindsey R.drake Allen F.Brooks Jenelle stauff Phillip s.sherman Janna Arteaga Robert A.Koeppe Aimee Reed Timothy J.Montavon Marc B.skaddan Peter J.H.scott Department of Radiology University of MichiganAnn ArborMI48109USA Department of Medicinal Chemistry University of MichiganAnn ArborMI48109USA AbbVie Process Chemistry North ChicagoIL60064USA AbbVie Centralized Medicinal Chemistry North ChicagoIL60064USA AbbVie Translational Imaging North ChicagoIL60064USA

The implication of the receptor for advanced glycation end-products(RAGE)in numerous diseases and neurodegenerative disorders makes it interesting both as a therapeutic target and as an inflammatory *** the context of investigating RAGE as a biomarker,there is interest in developing radiotracers that will enable quantification of RAGE using positron emission tomography(PET)*** have synthesized potential small molecule radiotracers for both the intracellular([18F]InRAGER)and extracellular([18F]RAGER)domains of *** we report preclinical evaluation of both using in vitro(lead panel screens)and in vivo(rodent and nonhuman primate PET imaging)*** radiotracers have high affinity for RAGE and show good brain uptake,but suffer from off-target *** source of the off-target PET signal is not attributable to binding to melatonin receptors,but remains *** have also investigated use of lipopolysaccharide(LPs)-treated mice as a possible animal model with upregulated RAGE for evaluation of new imaging *** of the mouse brain sections revealed increases in RAGE in the male cohorts,but no difference in the female ***,it proves challenging to quantify the changes in RAGE due to off-target binding of the ***,they are appropriate lead scaffolds for future development of 2nd generation RAGE PET radiotracers because of their high affinity for the receptor and good CNs penetration.

关键词： RAGE Neuroimaging Positron emission tomography Radiochemistry Neuroinflammation

Identification and Antimicrobial susceptibility of <i>Yersinia enterocolitica</i>Found in Chitterlings, Raw Milk and swine Fecal samples

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Advances in Microbiology 2018年第10期8卷 804-820页

作者： F. N. drake s. Davis J. Khatiwada L. Williams North Carolina A & T state University North Carolina Research Campus Kannapolis NC USA

Foodborne illness is an escalating concern upon public health. The prevalence of Yersinia enterocolitica was assessed in chitterlings, raw milk and swine fecal from North Carolina. Uncleaned thirty chitterling samples procured from a local grocery store, forty-five swine fecal samples, and forty unpasteurized cow milk samples supplied by the University farm were evaluated for the presence of Y. enterocolitica. Isolates identified as presumptive positive were characterized as colonies with a pink or deep-red center on MacConkey and CIN agar, and verified further through polymerase chain reaction (PCR) for the presence of 16s rRNA gene for the Yersinia genera. Results showed that 4.4% swine fecal samples, 7.5% milk samples and 11.3% chitterling samples were presumptive positive for Y. enterocolitica by the direct plating method on selective agars. Of the thirty-chitterling samples examined by PCR for the 16s rRNA gene, 26% samples contained the identification gene for the bacteria of interest. After conducting virulence tests, the fecal samples were revealed as non-pathogenic. Only one of the milk samples were considered pathogenic and consisted of the following virulent genes: Yersinia heat-stable toxin (yst), invasion (inv), attachment invasion locus (ail), virulence regulon transcriptional activator (virF), Yersinia adehesin A (yadA), and the O:3 antigen gene (rfbC). seven out of the eight (87.5%) chitterling samples were shown to be pathogenic. Disc diffusion was conducted to determine the antimicrobial susceptibility of the isolates. Over half (55.5%) of the antimicrobial agents were found effective, with isolates being completely susceptible to ciprofloxacin, kanamycin, trimethoprim, cefotaxime, and gentamycin. Ampicillin was determined to be least effective, where 84.6% of the samples presented resistance to the drug. Random amplified polymorphic DNA (RAPD) analysis and ERIC-PCR techniques were used to evaluate genetic similarity among the Yersinia isolates. Th

关键词： Yersinia enterocolitica Polymerase Chain Reaction Antimicrobial

strawberry sweetness and consumer preference are enhanced by specific volatile compounds

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Horticulture Research 2021年第1期8卷 870-884页

作者： Zhen Fan Tomas Hasing Timothy s.Johnson drake M.Garner Michael L.schwieterman Christopher R.Barbey Thomas A.Colquhoun Charles A.sims Marcio F.R.Resende Vance M.Whitaker Horticultural sciences Department University of FloridaIFAS Gulf Coast Research and Education CenterWimaumaFLUSA Elo Life systems DurhamNCUSA Department of Environmental Horticulture and Plant Innovation Center Institute of Food and Agricultural SciencesUniversity of FloridaGainesvilleFLUSA Plenty Unlimited Inc.South San FranciscoCA 94080USA Food science and Human Nutrition Department University of FloridaGainesvilleFLUSA Horticultural sciences Department University of FloridaGainesvilleFLUSA

Breeding crops for improved flavor is challenging due to the high cost of sensory evaluation and the difficulty of connecting sensory experience to chemical *** main goal of this study was to identify the chemical drivers of sweetness and consumer liking for fresh strawberries(Fragaria×ananassa).Fruit of 148 strawberry samples from cultivars and breeding selections were grown and harvested over seven years and were subjected to both sensory and chemical *** panel consisted of at least 100 consumers,resulting in more than 15,000 sensory data points per *** sugars,two acids and 113 volatile compounds were *** liking was highly associated with sweetness intensity,texture liking,and flavor intensity,but not sourness *** least square analyses revealed 20 volatile compounds that increased sweetness perception independently of sugars;18 volatiles that increased liking independently of sugars;and 15 volatile compounds that had positive effects on *** learning-based predictive models including sugars,acids,and volatiles explained at least 25%more variation in sweetness and liking than models accounting for sugars and acids *** compounds such asγ-dodecalactone;5-hepten-2-one,6-methyl;and multiple medium-chain fatty acid esters may serve as targets for breeding or quality control attributes for strawberry products.A genetic association study identified two loci controlling ester production,both on linkage group 6 ***-segregating makers in these regions can be used for increasing multiple esters *** study demonstrates a paradigm for improvement of fruit sweetness and flavor in which consumers drive the identification of the most important chemical targets,which in turn drives the discovery of genetic targets for marker-assisted breeding.

关键词： volatile sugar esters

Oral mucosal lipids are antibacterial against Porphyromonas gingivalis, induce ultrastructural damage, and alter bacterial lipid and protein compositions

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International Journal of Oral science 2013年第3期5卷 130-140页

作者： Carol L Fischer Katherine s Walters David R drake Deborah V Dawson Derek R Blanchette Kim A Brogden Philip W Wertz Dows Institute for Dental Research College of Dentistry The University of Iowa Central Microscopy Research Facility College of Dentistry The University of Iowa Department of Endodontics College of Dentistry The University of Iowa Department of Pediatric Dentistry College of Dentistry The University of Iowa Department of Periodontics College of Dentistry The University of Iowa Department of Oral Pathology Radiology & Medicine College of Dentistry The University of Iowa

Oral mucosal and salivary lipids exhibit potent antimicrobial activity for a variety of Gram-positive and Gram-negative bacteria;however,little is known about their spectrum of antimicrobial activity or mechanisms of action against oral *** this study,we examine the activity of two fatty acids and three sphingoid bases against Porphyromonas gingivalis,an important colonizer of the oral cavity implicated in *** inhibitory concentrations,minimal bactericidal concentrations,and kill kinetics revealed variable,but potent,activity of oral mucosal and salivary lipids against ***,indicating that lipid structure may be an important determinant in lipid mechanisms of activity against bacteria,although specific components of bacterial membranes are also likely *** micrographs showed ultrastructural damage induced by sapienic acid and phytosphingosine and confirmed disruption of the bacterial plasma *** information,coupled with the association of treatment lipids with *** lipids revealed via thin layer chromatography,suggests that the plasma membrane is a likely target of lipid antibacterial *** a combination of two-dimensional in-gel electrophoresis and Western blot followed by mass spectroscopy and N-terminus degradation sequencing we also show that treatment with sapienic acid induces upregulation of a set of proteins comprising a unique *** stress response,including proteins important in fatty acid biosynthesis,metabolism and energy production,protein processing,cell adhesion and *** or therapeutic lipid treatments may be beneficial for intervention of infection by supplementing the natural immune function of endogenous lipids on mucosal surfaces.

关键词： antimicrobial lipid fatty acid Porphyromonasgingivalis sphingoid base sphingolipid ultrastructure