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Diverse nucleotide substitutions in rice base editing mediated by novel TadA variants

Plant Communications 2024年第8期5卷 104-112页

作者： Man Yu Yongjie Kuang Chenyang Wang Xuemei Wu shaofang Li Dawei Zhang Wenxian sun Xueping Zhou Bin ren Huanbin Zhou state Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology China Agricultural UniversityBeijing 100193China scientific Observing and experimental station of Crop Pests in Guilin Ministry of Agriculture and Rural AffairsGuilin 541399China Ministry of education Key Laboratory for Bio-Resource and eco-environment College of Life ScienceState Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610064China state Key Laboratory of Vegetable Biobreeding National Engineering Research Center for VegetablesBeijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry ScienceBeijing 100097China state Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058China Key Laboratory of Gene editing Technologies(Hainan) Ministry of Agricultural and Rural AffairsSanya 572024China

CRIsPR-mediated base editors have been widely used to correct defective alleles and create novel alleles by artificial evolution for the rapid genetic improvement of *** editing capabilities of base editors strictly rely on the performance of various nucleotide modification *** with the welldeveloped adenine base editors(ABes),cytosine base editors(CBes)and dual base editors suffer from unstable editing efficiency and patterns at different genomic loci in rice,significantly limiting their ***,we comprehensively examined the base editing activities of multiple evolved TadA8e variants in *** found that both TadA-CDd and TadA-e27R/N46L achieved more robust C-to-T editing than previously reported hyperactive hAID*D,and TadA-CDd outperformed TadA-e27R/N46L.A C-to-G base editor(CGBe)engineered with TadA-CDd and OsUNG performed highly efficient C-to-G editing in rice compared with that of *** addition,a dual base editor constructed with a single protein,TadDe,enabled simultaneous,highly efficient C-to-T and A-to-G editing in ***,our results demonstrate that TadA8e derivatives improve both CBes and dual base editors in rice,providing a powerful way to induce diverse nucleotide substitutions for plant genome editing.

关键词： CRIsPR TadA variants cytosine base editing dual base editor rice

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source and level of dietary iron influence semen quality by affecting inflammation, oxidative stress and iron utilization levels in boars

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Journal of Animal science and Biotechnology 2024年第5期15卷 2044-2058页

作者： Yinghui Wu Yamei Li Yueyue Miao Hongkui Wei Hefeng Luo Chunxiao ren Yawei Zhang Juan Chen Tanghong Wei Jiyan Deng Jian Peng Department of Animal Nutrition and Feed science College of Animal Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China The Cooperative Innovation Center for sustainable Pig Production Wuhan 400700China Frontiers science Center for Animal Breeding and sustainable Production Wuhan 430070China Dekon Food and Agriculture Group Chengdu 610000China Xingjia Bio-engineering Co.Ltd Changsha 410011China

Background Boars fed a mixed form of inorganic and organic iron in excess of the NRC recommended levels still develop anemia,which suggested that the current level and form of iron supplementation in boar diets may be ***,56 healthy Topeka e line boars aged 15–21 months were randomly divided into 5 groups:basal diet supplemented with 96 mg/kg ferrous sulfate(FesO_(4))and 54 mg/kg glycine chelated iron(Gly-Fe,control);80 mg/kg or 115 mg/kg Gly-Fe;80 mg/kg or 115 mg/kg methionine hydroxyl analogue chelated iron(MHA-Fe,from CalimetFe)for 16 *** effects of dietary iron supplementation with different sources and levels on semen quality in boars were *** 1)serum Fe and hemoglobin concentrations were not affected by reduced dietary iron levels in the 80 mg/kg or 115 mg/kg Gly-Fe and MHA-Fe groups compared with the control group(P>0.05).2)serum interleukin-6(IL-6)and sperm malondialdehyde(MDA)levels in the 80 mg/kg or 115 mg/kg MHA-Fe groups were lower than those in the control group(Per serum superoxide dismutase levels and lower MDA levels in the 115 mg/kg MHA-Fe group(Ps in the 80 mg/kg or 115 mg/kg Gly-Fe and MHA-Fe groups had lower serum hepcidin(Perritin(Psferrin receptor(Pentrations,and boars in the 115 mg/kg MHA-Fe group had higher seminal plasma Fe concentrations compared with the control group.4)Boars in the 80 mg/kg and 115 mg/kg MHA-Fe groups had lower abnormal sperm rate and in situ oscillating sperm ratio compared to the control group at weeks 12 and/or 16 of the ***,the effect of Gly-Fe on improving semen quality in boars was not evident.5)serum IL-6 level was positively correlated with hepcidin concentration(Ps significantly positively correlated with abnormal sperm rate(Permore,significant correlations were also found between indicators of iron status and oxidative stress and semen quality ***

关键词： Adult boars Iron level Iron source Iron status semen quality

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single-cell RNA sequencing reveals abnormal transcriptome signature of erythroid progenitors in pure red cell aplasia

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Genes & Diseases 2024年第1期11卷 49-52页

作者： Yumei Liu Haiyue Niu Jie ren Zhiqin Wang Li Yan Limin Xing Zonghong shao Rong Fu Zhigang Cai Huaquan Wang Department of Hematology Tianjin Medical University General HospitalTianjin 300052China school of Basic Medical sciences Tianjin Medical UniversityTianjin 300070China

Pure red cell aplasia(PRCA)is a bone marrow failure syndrome characterized by severe anemia,reticulocytopenia,selective reduction,and even the absence of bone marrow erythroid precursors,while leukocyte and platelet counts are normal.1 Acquired PRCA can be divided into primary and *** are mediated by immune *** in the quantity and dysfunction of committed erythroid progenitors(ePs),including burst forming uniterythroid(BFU-e)and colony forming unit-erythroid(CFU-e),may be the source of reduced erythropoiesis and needs to be elucidated.

关键词： progenitor anemia leukocyte

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Using Copula functions to predict climatic change impacts on floods in river source regions

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Advances in Climate Change Research 2024年第3期15卷 406-418页

作者： Ting-Xing CHeN Hai-shen LYU Robert HORTON Yong-Hua ZHU ren-sheng CHeN Ming-Yue sUN Ming-Wen LIU Yu LIN The National Key Laboratory of Water Disaster Prevention College of Hydrology and Water ResourcesHohai UniversityNanjing 2098China College of Hydrology and Water Resources Hohai UniversityNanjing 210098China Departmentof Agronomy lowa State UniversityAmes IA5oollUSA Qilian Alpine eology and Hydrology Research station Northwest Instiuteof Eco-Environmentand ReourcesChinese Academy of SciencesLanzhou0000 China

Flood frequency in river source regions is significantly affected by rainfall and snowmelt as part of climatic changes.A traditional univariate flood frequency analysis cannot reflect the complexity of floods,and when used in isolation,it can only underestimate flood *** effective flood prevention and mitigation,it is essential to consider the combined effects of precipitation and *** functions can effectively quantify the joint distribution relationship between floods and their associated variables without restrictions on their distribution *** study uses copula functions to consider a multivariate probability distribution model of flood peak flow(Q)with cumulative snowmelt(Csm)and cumulative precipitation(CPr)for the Hutubi River basin located in northern Xinjiang,*** joint frequencies of rainfall and snowmelt floods are predicted using copula models based on the Coupled Model Intercomparison Project Phase 6 *** results show that Q has a significant positive correlation with 24-d Csm(r=0.559,p=0.002)and 23-d CPr(r=0.965,pequency will increase in the future,and mid-(2050e2074)and long-term(2075e2099)floods will be more severe than those in the near-term(2025e2049).The probability of flood occurrence is higher under the ssP2-4.5 and ssP1-2.6 scenarios than under *** during the historical period(1990e2014)led to extreme floods,and increasing future precipitation trends are found to be *** increases with rising temperatures and occurs earlier than estimated,leading to an earlier flood period in the basin and more frequent snowmelt *** Q under the joint return period is larger than that during the same univariate return *** difference indicates that neglecting the interaction between precipitation and snowmelt for floods leads to an underestimation of the flood risk(with underestimations ranging from 0.3%to 22%).The underestimations decrease with an increase

关键词： Climate change Flood frequency Copula CMIP6 River source regions

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Building Fe atom–cluster composite sites using a site occupation strategy to boost electrochemical oxygen reduction

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Carbon energy 2024年第3期6卷 276-286页

作者： Tingyi Zhou Yi Guan Changjie He Lei Zhang Xueliang sun Zhongxin song Qianling Zhang Chuanxin He Xiantao Jiang Zhaoyan Luo Wei Xing Xiangzhong ren College of Chemistry and environmental engineering Shenzhen UniversityShenzhenGuangdongChina Department of Mechanical and Materials engineering University of Western OntarioLondonOntarioCanada state Key Laboratory of electroanalytical Chemistry Jilin Province Key Laboratory of Low Carbon Chemical PowerChangchun Institute of Applied ChemistryChinese Academy of SciencesJilinChangchunChina

The high-temperature pyrolysis process for preparing M–N–C single-atom catalyst usually results in high heterogeneity in product structure concurrently contains multiscale metal phases from single atoms(sAs),atomic clusters to ***,understanding the interactions among these components,especially the synergistic effects between single atomic sites and cluster sites,is crucial for improving the oxygen reduction reaction(ORR)activity of M–N–C ***,herein,we constructed a model catalyst composed of both atomically dispersed FeN4 sA sites and adjacent Fe clusters through a site occupation *** found that the Fe clusters can optimize the adsorption strength of oxygen reduction intermediates on FeN4 sA sites by introducing electron-withdrawing–OH ligands and decreasing the d-band center of the Fe *** as-developed catalyst exhibits encouraging ORR activity with halfwave potentials(e1/2)of 0.831 and 0.905 V in acidic and alkaline media,***,the catalyst also represents excellent durability exceeding that of Fe–N–C sA *** practical application of Fe(Cd)–CNx catalyst is further validated by its superior activity and stability in a metalair battery *** work exhibits the great potential of synergistic effects between multiphase metal species for improvements of singleatom site catalysts.

关键词： d-band center metal clusters oxygen reduction reaction single-atom catalyst site occupations strategy

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Biochar with self-doped N to activate peroxymonosulfate for bisphenol-A degradation via electron transfer mechanism:The active edge graphitic N site

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Chinese Chemical Letters 2024年第9期35卷 351-355页

作者： Yun-Xin Huang Lin-Qian Yu Ke-Yu Chen Hao Wang shou-Yan Zhao Bao-Cheng Huang ren-Cun Jin school of engineering Hangzhou Normal UniversityHangzhou 311121China Zhejiang Provincial Key Laboratory of Urban Wetlands and Regional Change Hangzhou 311121China

environmental endocrine disruptors,represented by bisphenol A(BPA),have been widely detected in the environment,bringing potential health risks to human ***-containing biocarbon catalyst can activate peroxymonosulfate(PMs)to degrade BPA in water,but its active sites remain ***,in this work,nitrogen-containing biochar,i.e.,C–Nedge,enriched with graphitic-N defects at the edges was prepared by one-pot co-pyrolysis of chitosan and potassium *** results showed that the C–Nedge/PMs system can effectively degrade 98%of BPA(50 mg/L).The electron transfer based non-radical oxidation mechanism was responsible for BPA *** graphitic-N doping endows biochar with strong electron transfer *** catalyst had good recovery and reuse *** catalytic oxidation was also feasible for other refractory pollutants removal and worked well for treating practical *** work may provide valuable information in unraveling the N doping configurationactivity relationship during activating PMs by biochar.

关键词： Peroxymonosulfate Carbon-based catalysts edge graphitic-N Non-radicals Bisphenol A

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A tectono-thermal perspective on the petroleum generation,accumulation and preservation in the southern Ordos Basin, North China

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Petroleum science 2024年第3期21卷 1459-1473页

作者： Peng Yang Zhan-Li ren Jin-Hua Fu Hong-Ping Bao Hui Xiao Zheng shi Kun Wang Yuan-Yuan Zhang Wen-Hui Liu Wen-Hou Li state Key Laboratory of Continental Dynamics Department of GeologyNorthwest UniversityXi'an710069ShanxiChina National engineering Laboratory for exploration and Development of Low-Permeability Oil and Gas Fields Exploration and Development Research Institute of PetroChina Changqing Oilfield CompanyXi'an710018ShaanxiChina shaanxi Key Lab of Petroleum Accumulation Geology School of Earth Science and EngineeringXi'an Shiyou UniversityXian710065ShaanxiChina Wuxi Research Institute of Petroleum Geology Petroleum Exploration and Production Research InstituteSINOPECWuxi214126JiangsuChina

The southern Ordos Basin has excellent petroleum exploration ***,the tectono-thermal history and the controls on petroleum generation,accumulation and preservation conditions in southern basin are *** this study,we analyzed the present geothermal field,paleo-geothermal gradient,maturity of the hydrocarbon source rocks,uplift and cooling process and tectono-thermal evolution *** the study area,for the Ordovician,Permain and the Triassic strata,the present temperature is low(3070℃)in the southeastern area but high(80140℃)in the northwestern *** paleogeothermal gradient varied from 24℃/km to 30℃/km with a heat flow of 58—69 m W/m^(2)(i.e.,a medium-temperature basin).The paleo-temperatures are higher than the present temperatures and the maximum paleo-temperatures controlled the thermal maturity of the source *** vitrinite reflectance(Ro)values of the Triassic strata are>0.7%and the thermal maturity reached the middlemature oil generation *** Rovalues of the Permian-Ordovician strata are>1.8%and the thermal maturity reached the over-mature gas generation *** southern Ordos Basin has experienced the multiple tectonic events at the Late Ordovician Caledonian(452 Ma),Late Triassic(215 Ma),Late Jurassic(165160 Ma),end-early Cretaceous(110100 Ma)and Cenozoic(since 40 Ma).A large-scale tectonothermal event occurred at the end-early Cretaceous(110100 Ma),which was controlled by lithospheric extension,destruction and *** event led to the highest paleo-temperatures and thermal maturities and coeval with the peak period of petroleum generation and *** southern Ordos Basin has undergone rapid and large-scale uplift since the Late Cretaceous due to expansion of the northeastern margin of the Tibetan Plateau,uplift of the Qinling orogenic belt and thrust faulting in the Liupanshan tectonic *** southern Ordos Basin experienced tectonic overprinting that was strong in the south and weak in the *** str

关键词： Fission-track dating Vitrinitereflectance Geothermal field Tectono-thermal evolution Petroleum geology Ordos Basin

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selective Hydrodeoxygenation of Lignin-Derived Vanillin via Hetero-structured High-entropy Alloy/Oxide Catalysts

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energy & environmental Materials 2024年第3期7卷 202-210页

作者： Yan sun Kaili Liang ren Tu Xudong Fan Charles Q.Jia Zhiwen Jia Yingnan Li Hui Yang enchen Jiang Hanwen Liu Yonggang Yao Xiwei Xu Key Laboratory for Biobased Materials and energy of Ministry of education College of Materials and EnergySouth China Agricultural UniversityGuangzhou 510640China Department of Chemical engineering and Applied Chemistry University of Toronto200 College StreetToronto ON M5S3E5Canada state Key Laboratory of Materials Processing and Die&Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China

The chemoselective hydrodeoxygenation of natural lignocellulosic materials plays a crucial role in converting biomass into value-added *** their complex molecular structures often require multiple active sites synergy for effective activation and achieving high ***,it is reported that a high-entropy alloy(HeA)on high-entropy oxide(HeO)hetero-structured catalyst for highly active,chemoselective,and robust vanillin *** heterogenous HeA/HeO catalysts were prepared by thermal reduction of senary HeOs(NiZnCuFeAlZrO_(x)),where exsolvable metals(e.g.,Ni,Zn,Cu)in situ emerged and formed randomly dispersed HeA nanoparticles anchoring on the HeO *** catalyst exhibits excellent catalytic performance:100%conversion of vanillin and 95%selectivity toward high-value 2-methyl-4 methoxy phenol at low temperature of 120℃,which were attributed to the synergistic effect among HeO matrix(with abundant oxygen vacancies),anchored HeA nanoparticles(having excellent hydrogenolysis capability),and their intimate hetero-interfaces(showing strong electron transferring effect).Therefore,our work reported the successful construction of HeA/HeO heterogeneous catalysts and their superior multifunctionality in biomass conversion,which could shed light on catalyst design for many important reactions that are complex and require multifunctional active sites.

关键词： biomass conversion heterogeneous catalysts high-entropy oxide high-entropy alloys lignin pyrolysis

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A review:the mechanism of plant-derived polysaccharides on osteoblasts and osteoclasts

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未来食品学报(英文) 2024年第3期4卷 183-192页

作者： Mengjie ren Adel F.Ahmed Meng Li Menghan Li Zhiruo Yan Jinmei Wang National R&D Center for edible Fungus Processing Technology Henan UniversityKaifeng 475004China Joint International Research Laboratory of Food&Medicine Resource Function Henan ProvinceKaifeng 475004China Medicinal and Aromatic Plants Researches Department Horticulture Research InstituteAgricultural Research CenterGiza 12511Egypt

Bone loss and deterioration of bone microarchitecture would increase the bone fragility and fracture risk,leading to the *** and more evidences proved that plant-derived polysaccharides could have a remarkable influence on osteoblasts and osteoclasts,exerting anti-osteoporosis *** to the previous research,the extract of Cibotium barumoz,Achyranthes bidentata,Curculigo orchioides,epimedium brevicornum,Angelica sinensis,Polygonatum sibiricum,Dendrobium officinale,Morinda officinalis,Nelumbo mucifera,Diospyros kaki,Hordeum vulgare,Cistanche deserticola,Commiphora Myrrha and other plant-derived polysaccharides could benefit to the osteoblasts and *** essential mechanisms are mainly related to the activation or inhibition of many factors,including runt-related transcription factor 2(Runx2),B-catenin,osterix(Osx),activator protein-1(AP-1),osteocalcin(OCN/BGP),alkaline phosphatase(ALP),osteopontin(OPN),bone morphogenetic protein(BMP),phosphatidylinositol 3-kinase(PI3K)/C-Jun N-terminal kinase(JNK)/extracellular regulated protein kinase(eRK),osteoprotegerin(OPG),receptor activator of nuclear factor-kB(RANK),monocyte/macrophage colony-stimulating factor(M-CsF),tumor necrosis factor receptor-associated factor 6(TRAF-6),receptor activator of nuclear factor(NF)-KB ligand(RANKL),nuclear factor of activated T cells 1(NFATc1),c-Fos,matrix metallopeptidase-9(MMP-9),glycogen synthase kinase 3β(GsK3B)/B-catenin,nuclear factor e2-related factor 2(Nf2),as well as these related pathways,such as Wnt/p-catenin,BMP-2/sMAD1/5/8,PI3K/AKT,OPG/RANKL/RANK,NF-κB,MAPKs,*** plant-derived polysaccharides could improve the dynamic balance of bone formation and resorption through promoting the differentiation and maturation of osteoblast or inhibiting its *** reviewed plant-derived polysaccharides and their regulating mechanisms on the osteoclasts and osteoblasts provide the evidences for the development of osteoporosis therapeutics.

关键词： Osteoporosis Plant-derived polysaccharides Osteogenesis promotion Osteoclasts inhibition

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Data-driven systematic analysis of waterborne viruses and health risks during the wastewater reclamation process

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environmental science and ecotechnology 2024年第3期19卷 80-90页

作者： Jia-Xin Ma Xu Wang Yi-Rong Pan Zhao-Yue Wang Xuesong Guo Junxin Liu Nan-Qi ren David Butler Research Center for eco-environmental sciences Chinese Academy of SciencesBeijing100085China state Key Laboratory of UrbanWater Resource and environment School of Civil and Environmental EngineeringHarbin Institute of TechnologyShenzhenShenzhen518055China University of Chinese Academy of sciences Beijing100049China Centre for Water systems College of EngineeringMathematics and Physical SciencesUniversity of ExeterExeterEX44QFUnited Kingdom

Waterborne viral epidemics are a major threat to public *** interest in wastewater reclamation highlights the importance of understanding the health risks associated with potential microbial hazards,particularly for reused water in direct contact with *** study focused on identifying viral epidemic patterns in municipal wastewater reused for recreational applications based on long-term,spatially explicit global literature data during 2000e2021,and modelled human health risks from multiple exposure pathways using a well-established quantitative microbial risk assessment *** median viral loads in municipal wastewater ranged from 7.92×10^(4)to 1.4×10^(6)GC L^(-1)in the following ascending order:human adenovirus(HAdV),norovirus(NoV)GII,enterovirus(eV),NoV GI,rotavirus(RV),and severe acute respiratory syndrome coronavirus 2(sARsCoV-2).Following secondary or tertiary wastewater treatment,NoV GI,NoV GII,eV,and RV showed a relatively higher and more stable log reduction value with medians all above 0.8(84%),whereas sARsCoV-2 and HAdV showed a relatively lower reduction,with medians ranging from 0.33(53%)to 0.55(72%).A subsequent disinfection process effectively enhanced viral removal to over 0.89-log(87%).The predicted event probability of virus-related gastrointestinal illness and acute febrile respiratory illnesses in reclaimed recreational water exceeded the World Health Organization recommended recreational risk benchmark(5%and 1.9%,respectively).Overall,our results provided insights on health risks associated with reusing wastewater for recreational purposes and highlighted the need for establishing a regulatory framework ensuring the safety management of reclaimed waters.

关键词： Reclaimed water Waterborne virus Recreational Human health Data-driven analysis

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