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Comparative study on production,purification of penicillin by Penicillium chrysogenum isolated from soil and citrus samples

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Asian Pacific Journal of Tropical Biomedicine 2011年第1期1卷 15-19页

作者： s anto jeya dayalan Pramod Darwin Prakash s Department of Biotechnology Udaya School of Engineering

Objective:To explore various unexplored locations where Penicillium *** be available and study the production of penicillin from the isolated Penicillium *** different media with altered carbohydrate ***:The collected soil samples were screened for the isolation of Penicillium chrysogenum(***) by soil dilution *** isolated Penicillium species were further grown in different production media with changes in the carbohydrate *** extracted penicillin from various isolates was analyzed by HPLC for the efficacy of the *** the products were screened with various bacterial species including methicillin resistant staphylococcus aureus(MRsA).And the work was extended to find the possible action on MRsA,along with characterization using other ***:From the various soil and citrus samples used for analysis,only the soil sample from Government General Hospital of Bangalore,India,and sanjay Gandhi Hospital,Bangalore,India,showed some potential growth of the desired fungi *** production media showed varied range of growth of *** production of penicillin was obtained in maltose which proved maximum zone of inhibition during *** of penicillin on pathogens,like wild Escherichia coli strain,Klebsiella spp.,and MRsA,gave quite interesting results such as no activity on the later strain as it is *** data provided the analytical and confirmation details of the penicillin ***,the penicillin produced from the soil sample of Government General Hospital had the high milli absorbance unit of 441.5 mAu compared with that of the penicillin produced from sanjay Gandhi Hospital sample,8s.s2 ***,there was a considerable change in quantity of the penicillin produced from both the ***: The Penicillium *** be possibly rich in hospital contaminants and its *** research focuses on various unexplored sources o

关键词： Penicillium chrysogenum soil Citrus Penicillin Bioefficacy Antibiotic activity Penicillium spp. Production soil dilution plate staphy lococcus aureus Characterization Production media Zone of inhibition Carbohydrate source Isolation Efficacy Pathogen

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Enzymatic and non-enzymatic antioxidant, anti-inflammatory and anticancer activities of Floscopa scandens Lour

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Journal of Traditional Chinese Medicine 2020年第3期40卷 473-483页

作者： anto suganya Regis jeya Jothi Gabriel Department of Plant Biology and Biotechnology Loyola CollegeNungambakkamChennai-600034Tamil NaduIndia

OBJECTIVE: To explore the total phenolic and flavonoid content, enzymatic, non-enzymatic antioxidant properties, anti-inflammation and anticancer activities of hexane, ethyl acetate and methanol extracts of Floscopa scandens(F. scandens).METHODs: Non-enzymatic antioxidant activity was examined by 2, 2-diphenyl-1-picrylhydrazyl assay, nitric oxide scavenging assay, hydroxyl radical scavenging assay, reducing power assay, hydrogen peroxide scavenging assay, superoxide scavenging assay and metal chelating assay. Enzymatic antioxidant ability was screened for the antioxidant enzymes such as ascorbate oxidase, peroxidase, catalase and polyphenol oxidase. The anti-inflammatory property was proved with the inhibition of protein denaturation and protease inhibitory assays. In vitro anticancer activity was assessed by cell viability ***: Methanol extract contained high amount of phenols(198.41 mg catechol equivalent/gram extract) and flavonoids(101.70 mg quercetin equivalent/gram extract) showed higher activity than hexane and ethyl acetate extracts in all experiments. Fresh plant showed considerable enzymatic antioxidant ***: The results revealed that the methanol extracts of F. scandens could be used as a potential source of antioxidant, anti-inflammatory and anticancer bioactive compounds.

关键词： Anti-inflammatory agents Antineoplastic agents Antioxidant Floscopa scandens

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