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A novel saliva-based miRNA profile to diagnose and predict oral cancer

International Journal of Oral science 2024年第1期16卷 97-109页

作者： Jaikrishna Balakittnen Chameera Ekanayake Weeramange Daniel F.Wallace Pascal H.G.Duijf alexandre s.Cristino Gunter Hartel Roberto A.Barrero Touraj Taheri Liz Kenny sarju Vasani Martin Batstone Omar Breik Chamindie Punyadeera saliva&Liquid Biopsy Translational Laboratory Griffith Institute for Drug DiscoveryGriffith UniversityNathanQLDAustralia Department of Medical Laboratory sciences Faculty of Allied Health SciencesUniversity of JaffnaJaffnaSri Lanka Menzies Health Institute Griffith UniversityGold CoastQLDAustralia school of Biomedical sciences Faculty of HealthQueensland University of TechnologyBrisbaneQLDAustralia Centre for Cancer Biology Clinical and Health SciencesUniversity of South Australia&SA PathologyAdelaideSAAustralia Institute of Clinical Medicine Faculty of MedicineUniversity of OsloOsloNorway Department of Medical Genetics Oslo University HospitalOsloNorway Griffith Institute for Drug Discovery Griffith UniversityNathanQLDAustralia QIMR Berghofer Medical Research Institute Statistics UnitBrisbaneQLDAustralia school of Public Health The University of QueenslandBrisbaneQLDAustralia school of Nursing Queensland University of TechnologyBrisbaneQLDAustralia eResearch Research InfrastructureAcademic DivisionQueensland University of TechnologyBrisbaneQLDAustralia Department of Anatomical Pathology Royal Brisbane and Women’s HospitalHerstonQLDAustralia Faculty of Medicine The University of QueenslandBrisbaneQLDAustralia Royal Brisbane and Women’s Hospital Cancer Care ServicesHerstonQLDAustralia Department of Otolaryngology Royal Brisbane and Women’s HospitalHerstonQLDAustralia Department of Oral and Maxillofacial surgery Royal Brisbane and Women’s HospitalHerstonQLDAustralia

Oral cancer (OC) is the most common form of head and neck cancer. Despite the high incidence and unfavourable patient outcomes, currently, there are no biomarkers for the early detection of OC. This study aims to discover, develop, and validate a novel saliva-based microRNA signature for early diagnosis and prediction of OC risk in oral potentially malignant disorders (OPMD).The Cancer Genome Atlas (TCGA) miRNA sequencing data and small RNA sequencing data of saliva samples were used to discover differentially expressed miRNAs. Identified miRNAs were validated in saliva samples of OC (n=50), OPMD (n=52), and controls(n=60) using quantitative real-time PCR. Eight differentially expressed miRNAs (miR-7-5p, miR-10b-5p, miR-182-5p, miR-215-5p,miR-431-5p, miR-486-3p, miR-3614-5p, and miR-4707-3p) were identified in the discovery phase and were validated. The efficiency of our eight-miRNA signature to discriminate OC and controls was:area under curve (AUC):0.954, sensitivity:86%, specificity:90%,positive predictive value (PPV):87.8%and negative predictive value (NPV):88.5%whereas between OC and OPMD was:AUC:0.911,sensitivity:90%, specificity:82.7%, PPV:74.2%and NPV:89.6%. We have developed a risk probability score to predict the presence or risk of OC in OPMD patients. We established a salivary miRNA signature that can aid in diagnosing and predicting OC,revolutionising the management of patients with OPMD. Together, our results shed new light on the management of OC by salivary miRNAs to the clinical utility of using miRNAs derived from saliva samples.

关键词： specificity salivary diagnosis

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simulated rainfall in Brazil:An alternative for assesment of soil surface processes and an opportunity for technological development

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International soil and Water Conservation Research 2024年第1期12卷 29-42页

作者： Camila Calazans da silva Luz Wilk sampaio de Almeida Adilson Pacheco de souza Nivaldo schultz Jamil alexandre Ayach Anache Daniel Fonseca de Carvalho Postgraduate Program in Agronomy-soil sciences Federal Rural University of Rio de JaneiroSeropédicaRJBrazil Agronomy Department Federal Institute of EducationScience and Technology of RondôniaAriquemesROBrazil Federal University of Mato Grosso SinopMTBrazil Federal Rural University of Rio de Janeiro SeropédicaRJBrazil Federal University of Mato Grosso do sul Campo GrandeMSBrazil

Rainfall simulators(Rs)have been used,above all,to evaluate hydrological processes related to soil water infiltration,surface runoff and soil *** allow repeatability of rain application with different precipitation intensities in field and/or laboratory conditions and should produce events with physical characteristics similar to natural *** this manuscript,we carried out a bibliometric and scientometric analysis of studies with simulated rainfall in Brazil to assess the temporal evolution of publications,the main topics addressed and the degree of technological development of the *** searched for the terms“rainfall simulator”OR“simulated rainfall”AND“Brazil”in the scopus,Web of science,sciELO and Google scholar *** found 143 articles published in the last 37 years(1985-2022).Our findings indicate that the main research areas covered in papers are soil erosion(57.34%),soil water infiltration(24.47%),nutrient losses(9.8%)and Rs development and calibration/assessment(8.39%).In recent years,the number of published papers in international high-impact factor journals has *** of the papers(49.65%)refer to studies carried out by institutions located in the south and southeast regions of ***,there is a large gap of studies on simulated rainfall in other regions of Brazil,where important biomes such as the Cerrado,Amazon,Caatinga,and their transitions are *** study informs research priorities on soil erosion under simulated rainfall and provides a bibliographic database that can assist in more detailed future analyses.

关键词： Rainfall simulator soil and water losses soil water infiltration Bibliometrics scientific review

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Cerebral and muscle tissue oxygenation during exercise in healthy adults: A systematic review

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Journal of sport and Health science 2024年第4期13卷 459-471,F0003页

作者： Diego Orcioli-silva Victor spiandor Beretta Paulo Cezar Rocha santos Felipe Marroni Rasteiro Anita Brum Marostegan Rodrigo Vitorio Claudio alexandre Gobatto Fulvia Barros Manchado-Gobatto Laboratory of Applied sport Physiology(LAFAE) School of Applied Sciences(FCA)University of Campinas(UNICAMP)Limeira 13484-350Brazil Posture and Gait studies Laboratory(LEPLO) Institute of BiosciencesSao Paulo State University(UNESP)Rio Claro 13506-900Brazil Physical Education Department School of Technology and SciencesSao Paulo State University(UNESP)Presidente Prudente 19060-900Brazil Department of Computer science&Applied Mathematics Weizmann Institute of ScienceRehovot 7610001Israel Center of Advanced Technologies in Rehabilitation Sheba Medical CenterRamat Gan 5265601Israel Department of sport Exercise and RehabilitationNorthumbria UniversityNewcastle upon Tyne NE18STUK

Background:Near-infrared spectroscopy(NIRs)technology has allowed for the measurement of cerebral and skeletal muscle oxygenation simultaneously during *** this technology has been growing and is now successfully used in laboratory and sports settings,this systematic review aimed to synthesize the evidence and enhance an integrative understanding of bloodﬂow adjustments and oxygen(O_(2))changes(i.e.,the balance between O_(2) delivery and O_(2) consumption)within the cerebral and muscle systems during ***:A systematic review was conducted using PubMed,Embase,scopus,and Web of science databases to search for relevant studies that simultaneously investigated cerebral and muscle hemodynamic changes using the near-infrared spectroscopy system during *** review considered manuscripts written in English and available before February 9,*** step of screening involved evaluation by 2 inde-pendent authors,with disagreements resolved by a third *** Joanna Briggs Institute Critical Appraisal Checklist was used to assess the methodological quality of the ***:Twenty studies were included,of which 80%had good methodological quality,and involved 290 young or middle-aged *** types of exercises were used to assess cerebral and muscle hemodynamic changes,such as cycling(n=11),treadmill(n=1),knee extension(n=5),isometric contraction of biceps brachii(n=3),and duet swim routines(n=1).The cerebral hemodynamics anal-ysis was focused on the frontal cortex(n=20),while in the muscle,the analysis involved vastus lateralis(n=18),gastrocnemius(n=3),biceps brachii(n=5),deltoid(n=1),and intercostal muscle(n=1).Overall,muscle deoxygenation increases during exercise,reaching a plateau in voluntary exhaustion,while in the brain,oxyhemoglobin concentration increases with exercise intensity,reaching a plateau or declining at the exhaustion ***:Muscle and cerebral oxygenation respond differently to exercise,with muscle

关键词： Near-infrared spectroscopy Hemodynamic Blood flow Brain Muscle metabolism

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Determination of Total Heavy Metals Content in Local Antidote Manufacturing Resources

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Open Journal of Applied sciences 2024年第8期14卷 2144-2153页

作者： alexandre Ngama Mwabi Pierre Yoniene Yassa Vestine Ntakarutimana Doctoral school University of Burundi Bujumbura Burundi Research Center in Natural science and Environment (CRsNE) University of Burundi Bujumbura Burundi Faculty of Medicine University of Kaziba (UNIKAZ) Bukavu Democratic Republic of Congo (DRC) Department of Chemistry Faculty of Sciences University of Burundi Bujumbura Burundi

The present work was initiated for searching and evaluating the total heavy metal content in some selected local resources used by traditional healers in locally made antidotes to search for their toxicity. Elekat public slaughterhouse in the city of Bukavu, the central market of Kadutu and in the Ruzizi plain, precisely in sange city, have been the purchased centers. The samples of bull and cow horns, oil palm nut shells, cow bones, ginger, black pepper and Mwenga salt were used. The spectrometric method has been used to carry out heavy metal content in each local resource using the atomic absorption spectrometer (VARIAN 1275). The heavy metals found and their average content are as follows: copper (Cu: 124.79 mg/kg), lead (Pb: 76.41 mg/kg), chromium (Cr: 33.9 mg/kg), zinc (Zn: 190.86 mg/kg), Arsenic (As: 107.41 mg/kg) and cadmium (Cd: 0.05 mg/kg). The averages of total heavy metal contents for all samples are below the standard of each one. The determination of the heavy metal contents in these samples allows evaluating human intoxication risk. These results show that antidote technology can be run without any toxicity risk and showed statistically significant differences compared to the controls (p 0.05) for chromium, zinc and cadmium have been recorded. However, most of the heavy metals molecules being thioloprives and then bioccumulatives, antidotes made from them shall not be consumed during a long period.

关键词： Heavy Metals Local Resources Contamination Antidote

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Chemical Analysis of Activated Carbon from Bull and Cow Horns Pyrolysis to Be Used as Antidotes

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Open Journal of Applied sciences 2024年第8期14卷 2133-2143页

作者： alexandre Ngama Mwabi Pierre Yoniene Yassa Vestine Ntakarutimana Doctoral school University of Burundi Bujumbura Burundi Research Center in Natural science and Environment (CRsNE) University of Burundi Bujumbura Burundi Faculty of Medicine University of Kaziba (UNIKAZ) Bukavu Eastern Democratic Republic of Congo (DRC) Department of Chemistry Faculty of Sciences University of Burundi Bujumbura Burundi

The purpose of this study is to compare the results of chemical analysis of two types of activated from the pyrolysis of bull horn and that of cow. six samples were used to measure pH, carbon, calcium and to determine adsorbent power. The pH was measured at a temperature of 20˚C using an “ANION 7010 ionomer” pH meter, the carbon (C) content was analyzed using a “EURO EA 3000” analyzer. and the electronic balance: “sartorius CP-2P”, calcium (Ca) was analyzed using a DFs-8 spectrograph. For the adsorbency test, the 0.15% methylene blue R solution was used. At the end of this study, we found that the activated carbon from the bull horn demonstrated a carbon content that is higher than that of the cow horn (20.79% against 15.63%), activated carbon of cow horn is richer in calcium than that of bull horn (16.27% against 3.69%) and then the pH. The cow horn is higher than that of the bull horn (7.43 versus 6.5). For the adsorbent power, the sample (75% bull horn and 25% cow horn) was recorded with the greatest adsorbent power. Thus, from this study, it can be recommended as an activated carbon antidote to be used for poisonings treatment.

关键词： Activated Carbon Bull Horn Cow Horn Oil Palm Nut shells Absorbent Power

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Human immunodeficiency virus cascade–continuum of care stages and outcomes in a hospital in southern Brazil

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World Journal of Virology 2024年第3期13卷 65-75页

作者： Manoela Badinelli Vaucher Patrícia Fisch Dimas alexandre Kliemann Department of Hepatology Universidade Federal de Ciências da Saúde de Porto AlegrePorto Alegre 90050-170Rio Grande do SulBrazil Department of Medicine Universidade do Vale do Taquari(UNIVATES)Lajeado 95914-014Rio Grande do SulBrazil Department of Life sciences Universidade de Santa Cruz do Sul(UNISC)Santa Cruz do Sul 96815-900Rio Grande do SulBrazil Department of Medicine Pontifícia Universidade Católica do Rio Grande do SulPorto Alegre 90619-900Rio Grande do SulBrazil Department of Epidemiology Hospital Nossa Senhora da ConceiçãoPorto Alegre 91350-250Rio Grande do SulBrazil Department of Infectious Disease Hospital Nossa Senhora da ConceiçãoPorto Alegre 91350-250Rio Grande do SulBrazil

BACKGROUND The human immunodeficiency virus(HIV)continuum of care cascade illustrates the 90-90-90 goals defined by the Joint United Nations Program on HIV/acquired immunodeficiency syndrome(UNAIDs).The care cascade includes the following five steps:Diagnosis,linkage to care,retention in care,adherence to antiretroviral therapy(ART),and viral *** To elaborate the HIV cascade of patients diagnosed with HIV at the Nossa senhora da Conceição Hospital(HNsC)and to determine possible local causes for the loss of patients between each step of the *** This retrospective cohort study included patients diagnosed with HIV infection from January 1,2015 to December 31,2016 and followed up until July 31,*** data were analyzed by IBM sPss software version 25,and Poisson regression with simple robust variance was used to analyze variables in relation to each step of the *** with Psis,and Ps considered ***’sχ^(2) test was used to compare the groups of patients followed up at the HNsC and those followed up at other *** The results were lower than those expected by the UNAIDs,with 94%of patients linked,91%retained,81%adhering to ART,and 84%in viral *** and site of follow-up were the variables with the highest statistical significance.A comparison showed that the cascade of patients from the HNsC had superior results than outpatients,with a significant difference in the last step of the *** The specialized and continued care provided at the HNsC was associated with better results and was closer to the goals set by the *** development of the HIV cascade using local data allowed for the stratification and evaluation of risk factors associated with the losses occurring between each step of the cascade.

关键词： Cascade Continuum care Human immunodeficiency virus Antiretroviral therapy Adherence

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Unsuccessful tuberculosis treatment outcomes across Brazil's geographical landscape before and during the COVID-19 pandemic:are we truly advancing toward the sustainable development/end TB goal?

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Infectious Diseases of Poverty 2024年第1期13卷 105-106页

作者： Reginaldo Bazon Vaz Tavares Thais Zamboni Berra Yan Mathias Alves Marcela Antunes Paschoal Popolin Antonio Carlos Vieira Ramos Ariela Fehr Tartaro Clara Ferreira de souza Ricardo alexandre Arcencic Department of Maternal-Infant and Public Health Nursing University of São Paulo at Ribeirão Preto College of Nursing (USP/RPCN) Ribeirão Preto São Paulo Brazil Federal University of Tocantins Palmas Campus (FUT) Palmas Brazil state University of Minas Gerais Passos Campus (SUMG) Passos Brazil

Background Tuberculosis is one of the most significant infectious diseases for global public *** reallocation of healthcare resources and the restrictions imposed by the COVID-19 pandemic have hindered access to TB diag-nosis and *** in unfavorable outcomes of the disease have been observed in *** objective of this study was to analyze the spatial distribution of unfavorable TB treatment outcomes in Brazil before and during the *** An ecological study with spatial analysis was conducted with all 5569 municipalities in *** reported cases of tuberculosis between January 2010 and December 2021,as well as reported cases of COVID-19 from Febru-ary 2020 to December 2021,were *** outcomes studied encompass loss to follow-up,drug-resistant tuber-culosis,and *** Getis Ord GI*technique was employed to assess spatial association,and the Kernel density estimator was used to identify areas with concentrated increases or decreases in *** Local Moran's/was used to examine the spatial association between outcomes and cOVID-19 *** study was approved by the Research Ethics Committee of Ribeirao Preto Nursing school,University of sao *** There were 134,394 cases of loss to follow-up,10,270 cases of drug resistance,and 37,863 *** of high and low values were identified for all three outcomes,indicating significant changes in the spatial distribu-tion *** in concentrations were observed for lost to follow-up cases in the southeast,while reductions occurred in the Northeast,south,and ***-resistant tuberculosis experienced an increase in the southern and southeastern regions and a decrease in the Northeast and ***-related deaths showed notable concentra-tions in the Midwest,Northeast,south,and *** was an increase in high occurrence clusters for deaths after 2020 and 2021 in the *** The pandemic has brought additional chal

关键词： Tuberculosis COVID-19 spatial analysis Ecological studies

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Assessment of Dose and Lifetime Risk of Exposure Induced Cancer in Adult Common Computed Tomography scans in Douala-Cameroon

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Open Journal of Radiology 2024年第3期14卷 135-146页

作者： Celestin Mpeke Mokubangele alexandre Ngwa Ebongue Daniel Bongue Boniface Moifo Centre for Atomic Molecular Physics and Quantum Optics (CEPAMOQ) University of Douala Douala Cameroon Department of Physics Faculty of Science University of Douala Douala Cameroon Department of Radiology and Radiation Oncology Faculty of Medicine and Biomedical Sciences University of Yaounde 1 Yaounde Cameroon Radiology Department Yaounde Gynaeco-Obstetric and Pediatric Hospital Yaounde Cameroon

Background: Among medical technologies that use ionizing radiation, CT is currently the radio diagnostic technic that can deliver the highest radiation to the Patient compared with other conventional procedures. In developing countries, the uses and risks of CT have not been well characterized. Objective: To estimate the lifetime attributable risk (LAR) incidence and mortality for cancer for each procedure for adult’s patients who had Computed Tomography examinations in 10 imaging centers in the city of Douala-Cameroon so as to provide a reference data. Materials and Methods: We conducted a cross-sectional study describing radiation dose associated with the 8 most common types of diagnostic CT studies performed on 1287 consecutive adult patients at 10 Douala radiology department. We estimated lifetime attributable risks of cancer by study type from these measured doses. Estimation of LAR for cancer incidence and mortality was based on the effective dose, patient’s sex and age at exposure using the BIER VII preferred models. Results: Mean effective dose from CT scans examinations varied from: 0.30 and 8.81 msv. The highest doses were observed for lumbar spine CT (8.81 msv), followed by abdomen-pelvis procedure (6.46 msv), chest-abdomen-pelvic CT (6.61 msv), chest CT (3.90 msv), cervical spine CT (3.05 msv), head CT (1.7 msv) and lower for sinus CT (0.30 msv). The LAR values of all cancer from patients’ CT scans obtained vary from 67.13 excess per 100,000 (about 1 in 1489) and 0.45 excess per 100,000 (about 1 in 222,222). All cancer risk was high for lumbar spine CT in women 20 years old (67.13 excess deaths in 100,000 scans) followed by chest-abdomen-pelvic CT (50.36 excess deaths in 100,000 scans) and abdomen-pelvic CT (49.22 excess deaths in 100,000 scans) for the same age group. The LAR of incidence and mortality values were higher from female’s patients than males and higher for younger than older patients. Conclusion: This study was set out to estimate the LAR v

关键词： Patient Dose CT scan BEIIR VII Report Cancer Risk Assessment

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Effect of Eu3+,Bi3+,and Li+ doping on luminescent property of GdNbO4

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Journal of Rare Earths 2024年第1期42卷 66-75页

作者： s.W.Wi Y.s.Lee Department of Physics and Integrative Institute of Basic sciences Soongsil University

We investigated the emission properties of Eu3+-doped GdNbO4-based oxide phosphors,aiming at improving the Eu3+emission by codoping of Bi3+and Li+.Eu3+singly doped,Eu3+and Bi3+doubly doped,and Eu3+,Bi3+,and Li+triply doped GdNbO4were prepared using a high-temperature solid-state reaction *** red-orange emissions from Eu3+in the singly doped samples are significantly improved by the codoping of Bi3+,mainly due to the energy transfer from Bi3+to Eu3+.The additional codoping of Li+is found to increase the Eu3+emission *** improvement might be attributed to the increase in the photoluminescent quantum yield originating from larger grain sizes and better crystallinity.A detailed analysis of the X-ray diffraction pattern and the asymmetric ratio estimated from the photoluminescence spectra show that the local lattice environment around Eu3+in GdNbO4does not change significantly with the codoping of Bi3+and Li+.Our results indicate that Li+doping is a promising way to improve the emission properties of rare-earth ion-doped GdNbO4phosphors.

关键词： Eu 3+ GdNbO 4 Codoping of Li + Photoluminescence sensitizer Rare earths

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Liver transplantation as an alternative for the treatment of perihilar cholangiocarcinoma: A critical review

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Hepatobiliary & Pancreatic Diseases International 2024年第2期23卷 139-145页

作者： Wellington Andraus Francisco Tustumi alexandre Chagas santana Rafael soares Nunes Pinheiro Daniel Reis Waisberg Liliana Ducatti Lopes Rubens Macedo Arantes Vinicius Rocha santos Rodrigo Bronze de Martino Luiz Augusto Carneiro D’Albuquerque Department of Gastroenterology Transplantation UnitUniversidade de São PauloSão PauloBrazil

Background:Perihilar cholangiocarcinoma(phCCC)is a dismal *** is no consensus regard-ing the best treatment for patients with unresectable *** present review aimed to gather the current pieces of evidence for liver transplantation and liver resection as a treatment for phCCC and to build better guidance for clinical *** sources:The search was conducted in PubMed,Embase,Cochrane,and *** related references were searched *** criteria were:reports in English or Portuguese literature that a)patients with confirmed diagnosis of phCCC;b)patients treated with a curative intent;c)patients with the outcomes of liver resection and liver *** reports,reviews,letters,editorials,conference abstracts and papers with full-text unavailability were excluded from the ***:Most of the current literature is based on observational retrospective studies with low grades of *** resection has better long-term outcomes than systemic chemotherapy or palliation ther-apy and liver transplantation is a good alternative for selected patients with unresectable *** candidates for resection or transplantation should be medically fit and free of intrahepatic or extrahep-atic *** a general rule,patients presenting with a tumor having a longitudinal size>3 cm or extending below the cystic duct,lymph node disease,confirmed extrahepatic dissemination;intraoper-atively diagnosed metastatic disease;a history of other malignancies within the last five years,and did not complete chemoradiation regimen and were medically unfit should not be considered for *** of these criteria should be individually *** transplantation or resection should only be considered in highly experienced hepatobiliary centers,and any decision-making must be based on a multidisciplinary ***:phCCC is a complex condition with high *** therapies,including hepatec-tomy and liv

关键词： Liver transplantation Cholangiocarcinoma Liver neoplasms

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