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Macrophages are the primary effector cells in IL-7-induced arthritis

Cellular & Molecular Immunology 2020年第7期17卷 728-740页

作者： Seung-jae kim Huan J.Chang Michael VVolin Sadiq Umar katrien Van Raemdonck Aimee Chevalier karol Palasiewicz John W.Christman Suncica Volkov Shiva Arami Mehrdad Maz Anjali Mehta ryan k.Zomorrodi David A.Fox Nadera Sweiss Shiva Shahrara Division of Rheumatology Jesse Brown VAMedical CenterChicagoIL 60612USA Department of Medicine Division of RheumatologyUniversity of Illinois at ChicagoChicagoIL 60612USA Department of Microbiology and Immunology Chicago College of Osteopathic MedicineMidwestern UniversityDowners GroveIL 60515USA Pulmonary Critical Care and Sleep MedicineOhio State University Wexner Medical CenterDavis Heart and Lung Research InstituteColumbusOH 43210USA Division of Allergy Clinical Immunology and RheumatologyThe University of Kansas Medical CenterKansas CityKS 66160USA Division of Rheumatology and Clinical Autoimmunity Center of Excellence University of MichiganAnn ArborMI 481096USA

Synovial macrophages are crucial in the development of joint inflammation and bone damage;however, the pathways that controlmacrophage remodeling in inflammatory M1 cells or bone-eroding osteoclasts are not fully understood. We determined thatelevated IL-7R/CD127 expression is the hallmark of rheumatoid arthritis (RA) M1 macrophages and that these cells are highlyresponsive to interleukin-7 (IL-7)-driven osteoclastogenesis. We established that lipopolysaccharide (LPS), interferon-γ (IFNγ), andtumor necrosis factor-α (TNFα), the classic M1 macrophage mediators, enhance IL-7R expression in RA and murine *** local expression of IL-7 provokes arthritis, predominantly through escalating the number of F480^(+)iNOS^(+) cells rather than CD3^(+)T cells. Ectopic LPS injection stabilizes IL-7-induced arthritis by increasing myeloid IL-7R expression, in part via IFNγ ***, in RAG−/− mice, IL-7-mediated arthritis is suppressed because of the reduction in myeloid IL-7R expression due to the lackof IFNγ. Moreover, the amelioration of IL-7-induced arthritis by anti-TNF therapy is due to a decrease in the number of cells in theunique F480^(+)iNOS^(+)IL-7R^(+)CCL5^(+) subset, with no impact on the F480^(+)Arginase^(+) cell or CD3^(+) T cell frequency. Consistent with thepreclinical findings, the findings of a phase 4 study performed with RA patients following 6 months of anti-TNF therapy revealedthat IL-7R expression was reduced without affecting the levels of IL-7. This study shifts the paradigm by discovering that IL-7-induced arthritis is dependent on F480^(+)iNOS^(+)IL-7R^(+)CCL5^(+) cell function, which activates TH-1 cells to amplify myeloid IL-7Rexpression and disease severity.

关键词： myeloid elevated inflammation

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Sclerostin antibody improves alveolar bone quality in the Hyp mouse model of X-linked hypophosphatemia

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International Journal of Oral Science 2023年第4期15卷 640-648页

作者： kelsey A.Carpenter Delia O.Alkhatib Bryan A.Dulion Elizabeth Guirado Shreya Patel Yinghua Chen Anne George ryan D.Ross Department of Anatomy&Cell Biology Rush University Medical CenterChicagoILUSA Department of Oral Biology The University of Illinois at ChicagoChicagoILUSA Department of Orthopedic Surgery Rush University Medical CenterChicagoILUSA Department of Microbial Pathogens and Immunity Rush University Medical CenterChicagoILUSA

X-linked hypophosphatemia (XLH) is a rare disease of elevated fibroblast growth factor 23 (FGF23) production that leads tohypophosphatemia and impaired mineralization of bone and teeth. The clinical manifestations of XLH include a high prevalence ofdental abscesses and periodontal disease, likely driven by poorly formed structures of the dentoalveolar complex, including thealveolar bone, cementum, dentin, and periodontal ligament. Our previous studies have demonstrated that sclerostin antibody (SclAb) treatment improves phosphate homeostasis, and increases long bone mass, strength, and mineralization in the Hyp mousemodel of XLH. In the current study, we investigated whether Scl-Ab impacts the dentoalveolar structures of Hyp mice. Male andfemale wild-type and Hyp littermates were injected with 25 mg·kg−1 of vehicle or Scl-Ab twice weekly beginning at 12 weeks of ageand euthanized at 20 weeks of age. Scl-Ab increased alveolar bone mass in both male and female mice and alveolar tissue mineraldensity in the male mice. The positive effects of Scl-Ab were consistent with an increase in the fraction of active(nonphosphorylated) β-catenin, dentin matrix protein 1 (DMP1) and osteopontin stained alveolar osteocytes. Scl-Ab had no effecton the mass and mineralization of dentin, enamel, acellular or cellular cementum. There was a nonsignificant trend towardincreased periodontal ligament (PDL) attachment fraction within the Hyp mice. Additional PDL fiber structural parameters were notaffected by Scl-Ab. The current study demonstrates that Scl-Ab can improve alveolar bone in adult Hyp mice.

关键词： alveolar impaired elevated

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Taming Reactive Oxygen Species:Mitochondria-Targeting Aggregation-Induced Emission Luminogen for Neuron Protection via Photosensitization-Triggered Autophagy

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CCS Chemistry 2022年第7期4卷 2249-2257页

作者： Chao Chen Ruoyao Zhang Jianyu Zhang Yufan Zhang Haoke Zhang Zaiyu Wang Xiaolin Huang Sijie Chen ryan T.k.kwok Jacky W.Y.Lam Dan Ding Ben Zhong Tang Department of Chemistry Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and ReconstructionInstitute for Advanced StudyState Key Laboratory of Molecular NanoscienceDivision of Life ScienceDepartment of Chemical and Biological EngineeringThe Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong 999077 State key Laboratory ofMedicinal Chemical Biology Key Laboratory of Bioactive MaterialsMinistry of EducationCollege of Life SciencesNankai UniversityTianjin 300071 Ming Wai Lau Centre for Reparative Medicine Karolinska InstitutetHong Kong 999077 Center for Aggregation-Induced Emission SCUT-HKUST Joint Research InstituteState Key Laboratory of Luminescent Materials and DevicesSouth China University of TechnologyGuangzhou 510640 Shenzhen Institute of Aggregate Science and Technology School of Science and EngineeringThe Chinese University of Hong KongShenzhen 518172 AIE Institute Guangzhou Development DistrictHuangpuGuangzhou 510530

Oxidative damage to cells leads to accumulated harmful wastes,which in turn aggravate the imbalance of reactive oxygen species(ROS)and related ***,provoking the cellular defense system against severe oxidation and maintaining ROS homeostasis are ***,we designed and synthesized a powerful mitochondria-targeting aggregation-induced emission photosensitizer(named DTCSPY)by maximal restriction of heat *** is demonstrated that taming ROS generation within mitochondria through photosensitization-triggered autophagy via DTCSPY achieved a better neuroprotective effect against oxidative damages than Nacety-L-cysteine and vitamin *** work not only provides a new way to design high-performance photosensitizers by regulating the photophysical property,but also verifies the concept that taming ROS can be used for cell protection against destructive oxidation,thereby displaying broad prospects for alleviating oxidation-related diseases and promoting cell-based therapy.

关键词： aggregation-induced emission mitochondrial targeting photosensitizer reactive oxygen species neuron protection

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Genomics and introgression： Discovery and mapping of thousands of species-diagnostic SNPs using RAD sequencing

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Current Zoology 2015年第1期61卷 146-154页

作者： Brian k. HAND Tyler D. HETHER ryan P. kOVACH Clint C. MUHLFELD Stephen J. AMISH Matthew C. BOYER Sean M. O'ROURkE Michael R. MILLER Winsor H. LOWE Paul A. HOHENLOHE Gordon LUIkART Flathead Lake Biological Station Fish and Wildlife Genomics Group University of Montana Poison MT 59860 USA Institute for Bioinformatics and Evolutionary Studies Department of Biological Sciences University of Idaho Moscow ID 83844 USA U. S. Geological Survey Northem Rocky Mountain Science Center Glacier National Park West Glacier Montana 59936 USA Fish and Wildlife Genomics Group Division of Biological Sciences The University of Montana Missoula Montana 59812USA Montana Fish Wildlife and Parks Kalispell Montana 59901 USA Department of Animal Science University of California Davis One Shields Avenue Davis CA 95616 USA University of Montana Division of Biological Sciences Missoula Montana 59812 USA

Invasive hybridization and introgression pose a serious threat to the persistence of many native species. Understand- ing the effects of hybridization on native populations （e.g., fitness consequences） requires numerous species-diagnostic loci dis- tributed genome-wide. Here we used RAD sequencing to discover thousands of single-nucleotide polymorphisms （SNPs） that are diagnostic between rainbow trout （RBT, Oncorhynchus mykiss）, the world＇s most widely introduced fish, and native westslope cutthroat trout （WCT, （9. clarkii lewisi） in the northern Rocky Mountains, USA. We advanced previous work that identified 4,914 species-diagnostic loci by using longer sequence reads （100 bp vs. 60 bp） and a larger set of individuals （n = 84）. We sequenced RAD libraries for individuals from diverse sampling sources, including native populations of WCT and hatchery broodstocks of WCT and RBT. We also took advantage of a newly released reference genome assembly for RBT to align our RAD loci. In total, we discovered 16,788 putatively diagnostic SNPs, 10,267 of which we mapped to anchored chromosome locations on the RBT genome. A small portion of previously discovered putative diagnostic loci （325 of 4,914） were no longer diagnostic （i.e., fixed between species） based on our wider survey of non-hybridized RBT and WCT individuals. Our study suggests that RAD loci mapped to a draft genome assembly could provide the marker density required to identify genes and chromosomal regions in- fluencing selection in admixed populations of conservation concern and evolutionary interest [Current Zoology 61 （1）： 146-154, 2015].

关键词： Conservation genetics Hybridization Invasive species Next generation sequencing Salmonid fish SNP discovery

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Canadian children’s and youth’s adherence to the 24-h movement guidelines during the COVID-19 pandemic: A decision tree analysis

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Journal of Sport and Health Science 2020年第4期9卷 313-321页

作者： Michelle D.Guerrero Leigh M.Vanderloo ryan E.Rhodes Guy Faulkner Sarah A.Moore Mark S.Tremblay Children’s Hospital of Eastern Ontario Research Institute OttawaON K1H 8L1Canada ParticipACTION TorontoON M5S 1M2Canada Child Health and Evaluative Sciences Hospital for Sick ChildrenTorontoON M5G 0A4Canada Behavioral Medicine Laboratory School of Exercise SciencePhysical and Health EducationUniversity of VictoriaVictoriaBC V8W 2Y2Canada School of kinesiology University of British ColumbiaVancouverBC V6T 1Z1Canada Department of Therapeutic Recreation Faculty of ChildFamilyand Community StudiesDouglas CollegeCoquitlamBC V3B 7X3Canada School of Health and Human Performance Dalhousie UniversityHalifaxNS B3H 4R2Canada Department of Pediatrics University of OttawaOttawaON K1H 8L1Canada

Purpose:The purpose of this study was to use decision tree modeling to generate profiles of children and youth who were more and less likely to meet the Canadian 24-h movement guidelines during the coronavirus disease-2019(COVID-19)***:Data for this study were from a nationally representative sample of 1472 Canadian parents(Meanage=45.12,SD=7.55)of children(511 years old)or youth(1217 years old).Data were collected in April 2020 via an online *** items assessed demographic,behavioral,social,micro-environmental,and macro-environmental *** decision trees of adherence and non-adherence to all movement recommendations combined and each individual movement recommendation(physical activity(PA),screen time,and sleep)were ***:Results revealed specific combinations of adherence and non-adherence *** associated with adherence to the recommendation(s)included high parental perceived capability to restrict screen time,annual household income ofCAD 100,000,increases in children’s and youth’s outdoor PA/sport since the COVID-19 outbreak began,being a boy,having parents younger than 43 years old,and small increases in children’s and youth’s sleep duration since the COVID-19 outbreak *** associated with non-adherence to the recommendation(s)included low parental perceived capability to restrict screen time,youth aged 1217 years,decreases in children’s and youth’s outdoor PA/sport since the COVID-19 outbreak began,primary residences located in all provinces except Quebec,low parental perceived capability to support children’s and youth’s sleep and PA,and annual household income ofCAD 99,***:Our results show that specific characteristics interact to contribute to(non)adherence to the movement behavior *** highlight the importance of targeting parents’perceived capability for the promotion of children’s and youth’s movement behaviors during challengin

关键词： Decision tree analysis Parental perceived capability Physical activity Screen time Sleep

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Dystrophic calcification and heterotopic ossification in fibrocartilaginous tissues of the spine in diffuse idiopathic skeletal hyperostosis(DISH)

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Bone Research 2020年第2期8卷 209-218页

作者： Dale E.Fournier Patti k.kiser ryan J.Beach S.Jeffrey Dixon Cheryle A.Séguin Health and Rehabilitation Sciences(Physical Therapy) Faculty of Health SciencesThe University of Western OntarioLondonON N6A 5B9Canada Bone and Joint Institute The University of Western OntarioLondonON N6G 2V4Canada Department of Laboratory Medicine and Pathology Schulich School of Medicine&DentistryThe University of Western OntarioLondonON N6A 5C1Canada Department of Physiology and Pharmacology Schulich School of Medicine&DentistryThe University of Western OntarioLondonON N6A 5C1Canada

Diffuse idiopathic skeletal hyperostosis(DISH) is a prevalent noninflammatory spondyloarthropathy characterized by ectopic mineral formation along the anterolateral aspect of the vertebral column, yet little is known about its underlying pathogenesis. Our objective was to evaluate the histopathological features and composition of ectopic mineral within spinal tissues affected by DISH in humans. Thoracic spine segments from six embalmed cadaveric donors(one female and five males;median age 82 years)meeting the radiographic diagnostic criteria for DISH were evaluated using radiological, histological, and physical analyses. Overall,the histological features of ectopic mineralization at individual motion segments were heterogeneous, including regions of heterotopic ossification and dystrophic calcification. Heterotopic ossifications were characterized by woven and lamellar bone,multifocal areas of metaplastic cartilage, and bony bridges along the anterior aspect of the intervertebral disc space. Dystrophic calcifications were characterized by an amorphous appearance, a high content of calcium and phosphorus, an X-ray diffraction pattern matching that of hydroxyapatite, and radiodensities exceeding that of cortical bone. Dystrophic calcifications were found within the anterior longitudinal ligament and annulus fibrosus in motion segments both meeting and not meeting the radiographic criteria for DISH. In summary, our findings indicate that in DISH, ectopic mineral forms along the anterior aspect of the spine by both heterotopic ossification and dystrophic calcification of fibrocartilaginous tissues. Although both types of ectopic mineralization are captured by current radiographic criteria for DISH, dystrophic calcification may reflect a distinct disease process or an early stage in the pathogenesis of DISH.

关键词： DISH ossification calcification

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Reversible lithium storage in sp^(2) hydrocarbon frameworks

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Journal of Energy Chemistry 2022年第3期31卷 161-167,I0006页

作者： Zhangxiang Hao Junrun Feng Yiyun Liu Liqun kang Bolun Wang Junwen Gu Lin Sheng Ruoyu Xu Sushila Marlow Dan J.L.Brett Yunhui Huang Feng ryan Wang Materials and Catalysis Laboratory Department of Chemical EngineeringUniversity College LondonLondon WC1E 7JEUnited Kingdom School of Materials Science and Engineering Huazhong University of Science and TechnologyWuhan 430074HubeiChina Electrochemical Innovation Lab Department of Chemical EngineeringUniversity College LondonLondon WC1E 7JEUnited Kingdom Department of Chemistry University College LondonLondon WC1H 0AJUnited Kingdom

Polymer materials offer controllable structure-dependent performances in separation,catalysis and drug *** molecular structures can be precisely tailored to accept Li^(+)for energy storage *** the design of sp^(2)carbon-based polyphenylene(PPH)with high lithium-ion uptakes and long-term stability is ***-PPH(L-PPH)exceeds the performance of crosslink-PPH(C-PPH),due to the fact that it has an ordered lamellar structure,promoting the Li^(+)intercalation/deintercalation *** L-PPH cell shows a clear charge and discharge plateau at 0.35 and 0.15 V ***^(+)/Li,respectively,which is absent in the C-PPH *** Li^(+)storage capacity of L-PPH is five times that of the *** reversible storage capacity is further improved to 261 m Ah g;by functionalizing the L-PPH with the–SO_(3)H *** addition,the Li-intercalated structures of C-PPH and L-PPH are investigated via near-edge X-ray absorption fine structure(NEXAFS),suggesting the high reversible Li^(+)–C=C bond interaction at *** strategy,based on new insight into sp^(2)functional groups,is the first step toward a molecular understanding of the structure storage-capacity relationship in sp^(2)carbon-based polymer.

关键词： Lithium-ions battery Organic anode Topological structure sp^(2)hydrocarbon

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Dominance network structure across reproductive contexts in the cooperatively breeding cichlid fish Neolamprologus pulcher

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Current Zoology 2015年第1期61卷 45-54页

作者： Cody J. DEY Q.Y. Joanne TAN Constance M. O'CONNOR Adam R. REDDON J. ryan CALDWELL Sigal BALSHINE Department of Biology McMaster University Hamilton Ontario ON L8S 4K1 Canada Department of Psychology Neuroscience and Behaviour McMaster University Hamilton Ontario L8S 4L8 Canada

While a large number of studies have described animal social networks, we have a poor understanding of how these networks vary with ecological and social conditions. For example, reproductive periods are an important life-history stage that may involve changes in dominance relationships among individuals, yet no study to date has compared social networks of do- minance interactions （i.e. dominance networks） across reproductive contexts. We first analyzed a long-term dataset on captive so- cial groups of the cooperatively breeding cichlid Neolamprologuspulcher, and found that eviction events were significantly more common around reproduction than expected by chance. Next, we compared the structure of dominance networks during early pa- rental care and non-reproductive periods, using one of the first applications of exponential random graph models in behavioral biology. Contrary to our predictions, we found that dominance networks showed few changes between early parental care and non-reproductive periods. We found no evidence that dominance interactions became more skewed towards larger individuals, became more frequent between similar-sized individuals, or became more biased towards a particular sex during parental care. However, we did find that there were relatively more dominance interactions between opposite-sex dyads in the early parental care period, which may be a by-product of increased sexual interactions during this time. This is the first study in behavioral ecology to compare social networks using exponential random graph modeling, and demonstrates a powerful analytical framework for future studies in the field [Current Zoology 61 （1）： 45-54, 2015].

关键词： Social network Aggression Submissive Hierarchy Parental care P* model

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Filamentous Green Algae Reduce Walleye Fingerling Production in Earthen-Substrate Ponds

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Open Journal of Animal Sciences 2021年第2期11卷 157-163页

作者： Matthew J. Ward ryan A. Rasmus South Dakota Department of Game Fish and Parks Blue Dog State Fish Hatchery Waubay USA

Reduced walleye (Sander vitreus) fingerling production possibly related to nuisance filamentous green algae and overly stable water chemistry patterns is a concern in earthen-substrate ponds at Blue Dog State Fish Hatchery, South Dakota, USA. We describe the success of alfalfa (Medicago sativa) meal (AFM, n = 2), alfalfa meal plus soybean (Glycine max) meal (AFM + SBM, n = 1), and alfalfa meal plus liquid 28N:0P:0K (AFM + 28:0:0, n = 1) at achieving walleye production objectives (>104,000 walleye and 32 kilograms/hectare) as well as the occurrence of filamentous green algae and the associated patterns of am

关键词： Filamentous Green Algae Walleye Pond Culture

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A versatile AIE fluorogen with selective reactivity to primary amines for monitoring amination,protein labeling,and mitochondrial staining

聚集体(英文)

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聚集体(英文) 2023年第1期4卷 250-259页

作者： Xinyuan He Huilin Xie Lianrui Hu Pengchao Liu Changhuo Xu Wei He Wutong Du Siwei Zhang Hao Xing Xinyue Liu Hojeong Park Tsz Shing Cheung Min-Hui Li ryan T.k.kwok Jacky W.Y.Lam Jian Lu Ben Zhong Tang Department of Chemistry Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and ReconstructionState Key Laboratory of Molecular NeuroscienceDivision of Life Science and Department of Chemical and Biological EngineeringThe Hong Kong University of Science and TechnologyKowloonHong KongChina Department of Mechanical Engineering City University of Hong KongGreater Bay Joint DivisionShenyang National Laboratory for Materials ScienceKowloonHong KongChina Chimie ParisTech PSL University Paris CNRSInstitut de Recherche de Chimie ParisParisFrance Department of Chemistry Guangdong-Hongkong-Macou Joint Laboratory of Optoelectronic and Magnetic Functional MaterialsThe Hong Kong University of Science and TechnologyKowloonHong KongChina Center for Aggregation-Induced Emission SCUT-HKUST Joint Research InstituteState Key Laboratory of Luminescent Materials and DevicesSouth China University of TechnologyGuangzhouChina School of Science and Engineering Shenzhen Key Laboratory of Functional Aggregate MaterialsThe Chinese University of Hong KongShenzhen CityGuangdongChina

Specific bioconjugation for native primary amines is highly valuable for both chemistry and biomedical *** all the efforts,scientists lack a proper strategy to achieve high selectivity for primary amines,not to mention the requirement of fast response in real ***,we report a chromone-based aggregation-induced emission(AIE)fluorogen called CMVMN as a self-reporting bioconjugation reagent for selective primary amine identification,and its applications for monitoring bioprocesses of amination and protein *** is AIE-active and capable of solid-state ***,its electrospun films are manufactured for visualization of amine diffusion and leakage *** also shows good biocompatibility and potential mitochondria-staining ability,which provides new insight for organelle-staining probe *** with its facile synthesis and good reversibility,CMVMN would not only show wide potential applications in biology,but also offer new possibilities for molecular engineering.

关键词： aggregation-induced emission(AIE) bioconjugation mitochondria-staining primary amines protein labeling

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