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储藏温度对生姜保鲜效果的影响

江西中医药大学学报 2024年第4期36卷 58-64页

作者：孟儒缘吴毅刘微饶小勇罗晓健张爱玲何雁江西中医药大学南昌330004 江西省食品药品检验所/江西省药品与医疗器械质量工程技术研究中心南昌330046 中药固体制剂制造技术国家工程研究中心南昌330006

目的:考察储贮藏藏温度对生姜保鲜的影响,以确定最佳贮储藏藏温度。方法:利用低场核磁共振、质构仪、气相色谱-质谱联用技术对不同储藏温度下生姜保鲜效果进行评价。结果:生姜依据生长周期可分为姜母、子姜、孙姜等部位,不同部位品质具... 详细信息

目的:考察储贮藏藏温度对生姜保鲜的影响,以确定最佳贮储藏藏温度。方法:利用低场核磁共振、质构仪、气相色谱-质谱联用技术对不同储藏温度下生姜保鲜效果进行评价。结果:生姜依据生长周期可分为姜母、子姜、孙姜等部位,不同部位品质具有差异性,姜母、子姜、孙姜各部位水分状态不同,其中孙姜A_(23)最高,姜母A_(22)最高;孙姜硬度最大,姜母硬度最低。随贮藏时间的推移,不同部位生姜的自由水转化为束缚水;生姜硬度呈先增后降的趋势;生姜中烯烃类挥发性成分增加,醇类、醛类成分减少。与储存0 d相比,15℃贮藏温度生姜水分状态基本保持不变,品质变化最小,25℃或4℃储存都会导致自由水和束缚水的快速迁移,结合水基本不受温度影响;储藏温度在255℃℃时时,,生姜易腐败变质硬度下降,,4℃条件下,生姜遭受冷害质地变软;储存温度的变化对生姜挥发油中主要成分(烯烃类)无显著性影响。结论:储藏温度对生姜品质具有显著影响,在相对湿度95%、15℃条件下,生姜短期贮藏保鲜效果最好,但要实现生姜的长期储藏,需要研究新的保鲜技术和方法。

关键词：生姜储藏温度低场核磁水分迁移硬度质构保鲜

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人工诱导东北林蛙苏醒适应机制分析

安徽农学通报 2024年第11期30卷 49-55页

作者：赵红蕾张传辉孟如愿马佳欣文仁霜于翠平东北林业大学野生动物与自然保护地学院黑龙江哈尔滨150040

为了探究东北林蛙在人工诱导苏醒过程中适应温度变化的生理机制,包括不同温度、不同器官或组织的冷冻保护剂含量变化及其抗氧化应激相关生理指标在苏醒适应阶段的差异,采用梯度升温方法对冬眠东北林蛙进行人工诱导苏醒,至东北林蛙摄食... 详细信息

为了探究东北林蛙在人工诱导苏醒过程中适应温度变化的生理机制,包括不同温度、不同器官或组织的冷冻保护剂含量变化及其抗氧化应激相关生理指标在苏醒适应阶段的差异,采用梯度升温方法对冬眠东北林蛙进行人工诱导苏醒,至东北林蛙摄食标记苏醒过程结束。苏醒培养期间取样,未取样林蛙持续培养,取样结束后剩余林蛙常温养殖;采用分光光度法对其肝脏、肌肉内的葡萄糖含量、尿素氮含量、总抗氧化能力进行测定。结果表明,东北林蛙肝脏内上述物质含量与肌肉内各物质的消耗趋势基本一致,但肝脏内各物质含量及其变化幅度远大于肌肉内。冬眠东北林蛙人工诱导苏醒初期葡萄糖、尿素氮含量即冷冻保护剂含量明显降低(P<0.05)、总抗氧化能力明显增强(P<0.05),而苏醒后期,各项含量趋于稳定,无明显变化。这说明肝脏是东北林蛙冬眠过程中对抗低温冷冻环境的主要器官,且冬眠状态的东北林蛙体内会积累冷冻保护剂,随着苏醒过程的进行,温度逐渐上升,机体活动逐渐恢复,葡萄糖被大量消耗,以对抗因缺少能量来源而带来的应激损伤,抗氧化应激能力也随之增强以维持内环境稳定,保证东北林蛙在0℃以下得以生存。

关键词：东北林蛙人工诱导苏醒冷冻保护剂抗氧化能力人工养殖

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Loganin regulates glycolipid metabolism by influencing intestinal microbiota and AMPK signaling in obese mice

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Journal of Traditional Chinese Medical Sciences 2022年第3期9卷 321-329页

作者： Bingrui Xu Zimengwei Ye Tian Tian ruyuan Zhu Chenyue Liu Xin Fang Dongwei zhang Min Fu Sihua Gao Dandan Zhao School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing102488China Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing100700China Department of Endocrinology Beijing University of Chinese Medicine Third Affiliated HospitalBeijing100029China Research Institute of McGill University Health Center McGill UniversityMontrealH4A 3J1Canada

Objective: We aimed to observe the effects of loganin(Log) on serum glycolipid levels and probe the mechanisms focusing on intestinal flora and AMP-activated protein kinase(AMPK) signaling in obese ***: A high-fat diet was given for 12 consecutive weeks to generate the obesity model in institute of cancer research(ICR) mice. Body weight was measured weekly and fasting blood glucose(FBG) was determined every 2 weeks. Both the oral glucose tolerance test and the intraperitoneal insulin tolerance test were performed. The serum levels of total cholesterol(TC), triglyceride, high-density lipoproteincholesterol, low-density lipoprotein-cholesterol(LDL-C), and free fatty acids(FFA) were measured. The expression of key proteins in the AMPK signaling pathway in skeletal muscle tissue was detected by immunoblotting, and gut microbiota were characterized using 16S rDNA ***: Log significantly decreased the body weight and the FBG in obese mice(P <.05), and it could restore FBG to normal levels. The total cholesterol, LDL-C, and FFA levels were significantly reduced by Log compared with the obese controls(TC: P =.0020;LDL-C: P =.0233;FFA: P =.0127), and the glucose tolerance of animals was significantly improved(P =.0477). The western blot results showed that Log could upregulate the protein expression of Adenosine 5‘-monophosphate(AMP)-activated protein kinase(AMPKa), Sirtuin 1(SIRT1), and peroxisome proliferator-activated receptor-gamma coactivator-1 alpha(PGC1a) in skeletal muscle tissue of obese mice. 16S rDNA sequencing indicated that Log reduced the diversity of the gut flora in feces and altered the floral composition of obese ***: Log was effective in reducing body weight and improving glucolipid metabolism in obese mice, probably through activating AMPK signaling and regulating intestinal microbial diversity.

关键词： Loganin Insulin resistance Obesity Glucose metabolism Lipid metabolism Gut microbiota AMPK signaling Pathway Mechanisms

金水宝对去卵巢小鼠骨质疏松的保护作用及其机制

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现代中西医结合杂志 2024年第13期33卷 1753-1756,1893页

作者：刘海霞朱如愿马嘉轶鲁欣悦赵雨坤张晓乐张治国陈彦静中国中医科学院中医基础理论研究所北京100700 陕西省中医医院陕西西安710003

目的探讨金水宝对去卵巢小鼠骨质疏松的保护作用及其机制。方法将30只雌性C57BL/6J小鼠随机分为对照组、模型组、金水宝组,每组10只。模型组和金水宝组小鼠采用去卵巢方法构建绝经后骨质疏松症模型,造模第10天开始,金水宝组给予金水宝悬... 详细信息

目的探讨金水宝对去卵巢小鼠骨质疏松的保护作用及其机制。方法将30只雌性C57BL/6J小鼠随机分为对照组、模型组、金水宝组,每组10只。模型组和金水宝组小鼠采用去卵巢方法构建绝经后骨质疏松症模型,造模第10天开始,金水宝组给予金水宝悬液386 mg/kg灌胃,对照组和模型组给予等体积双蒸水灌胃,均1次/d,每周连续灌胃6 d,连续灌胃15周。采用micro-CT检测小鼠的股骨微结构(骨体积分数、骨小梁数量、骨小梁分离度)及骨密度,采用ELISA法测定小鼠血清I型胶原C端肽(CTX-I)、I型原胶原N端前肽(PINP)、骨碱性磷酸酶(BALP)和抗酒石酸酸性磷酸酶(TRAP)水平,采用Western blot法检测小鼠骨组织中低氧诱导因子-2α(HIF-2α)和肿瘤坏死因子受体相关因子6(TRAF6)的蛋白表达情况。结果与对照组比较,模型组小鼠股骨微结构破坏,骨体积分数、骨小梁数量、骨密度均明显降低(P均<0.05),骨小梁分离度及血清CTX-I、PINP、BALP、TRAP水平和骨组织中HIF-2α、TRAF6蛋白相对表达量均明显升高(P均<0.05);与模型组比较,金水宝组小鼠股骨微结构破坏减轻,骨体积分数、骨小梁数量、骨密度均明显升高(P均<0.05),骨小梁分离度及血清CTX-I、PINP、BALP、TRAP水平和骨组织中HIF-2α、TRAF6蛋白相对表达量均明显降低(P均<0.05)。结论金水宝可有效改善去卵巢小鼠骨质疏松,机制可能与抑制HIF-2α/TRAF6信号通路,调节骨代谢相关。

关键词：金水宝骨质疏松症去卵巢低氧诱导因子-2α 肿瘤坏死因子受体相关因子6 骨代谢

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Leaf nutrient resorption of two life-form tree species in urban gardens and their response to soil nutrient availability

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Leaf nutrient resorption of two life-form tree species in ur...

第十二届现代生态学讲座暨林草与环境学科研究生学术论坛

作者：郭晋平 ruyuan Hu Tairui Liu Yunxiang zhang Rongrong Zheng 山西农业大学林学院 College of Forestry Shanxi Agricultural University

Background. Leaf nutrient resorption is a key strategy in plant conservation that minimizes nutrient loss and enhances productivity. However,the differences of the nutrient resorption among garden tree species in urban ecosystems were not clearly understood,especially the differences of nitrogen resorption efficiency(NRE) and phosphorous resorption efficiency(PRE) between evergreen and deciduous ***. We selected 40 most generally used garden tree specie belonged two life forms(evergreen and deciduous) and investigated the nitrogen(N) and phosphorus(P) concentrations in green and senesced leaves and soil nutrient concentrations of nine samples trees for each ***,the nutrient concentrations and resorption efficiency were compared,and the soil nutrients utilization strategies were further ***. The results showed that the N concentration was significantly higher in the green and senesced leaves of deciduous trees than in the leaves of evergreen trees. The two life-form trees were both N limited and evergreen trees were more sensitive to N limitation. The NRE and PRE in the deciduous trees were significantly higher than those in the evergreen trees. The NRE was significantly positively correlated with the PRE in the deciduous trees. As the soil N and P concentrations increased,the nutrient resorption efficiency(NuRE) of the evergreen trees increased,but that of the deciduous trees decreased. Compared with the deciduous trees,the evergreen trees were more sensitive to the feedback of soil N and P concentrations. These findings reveal the N and P nutrient resorption mechanism of evergreen and deciduous trees and fill a gap in the understanding of nutrient resorption in urban ecosystems.

关键词：

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四极发电机定子绕组端部振动特性

热力发电 2014年第9期43卷 133-136页

作者：杨璋李如源福建宁德核电有限公司福建宁德315200

根据某台国产1 000MW核能机组四极发电机定子绕组端部出厂模态的试验结果及机组调试阶段的实测振动数据,探讨了其振动特性,并将其与相同容量的二极发电机进行了比较。结果表明:该四极发电机定子绕组端部固有频率有效避开了100Hz电磁激... 详细信息

根据某台国产1 000MW核能机组四极发电机定子绕组端部出厂模态的试验结果及机组调试阶段的实测振动数据,探讨了其振动特性,并将其与相同容量的二极发电机进行了比较。结果表明:该四极发电机定子绕组端部固有频率有效避开了100Hz电磁激励频率,具有良好的动态特性;其在额定转速1 500r/min空载、三相短路试验、全励磁试验、额定负荷工况下的振动最大幅值≤24μm,振动状态良好;与国产相同容量二极发电机相比,其转速减半,绕组端部的电动力也减半。

关键词： 1000MW核能机组四极发电机定子绕组端部振动特性

Specific Regulation of m^(6)A by SRSF7 Promotes the Progression of Glioblastoma

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Genomics, Proteomics & Bioinformatics 2023年第4期21卷 707-728页

作者： Yixian Cun Sanqi An Haiqing Zheng Jing Lan Wenfang Chen Wanjun Luo Chengguo Yao Xincheng Li Xiang Huang Xiang Sun Zehong Wu Yameng Hu Ziwen Li Shuxia zhang Geyan Wu Meisongzhu Yang Miaoling Tang ruyuan Yu Xinyi Liao Guicheng Gao Wei Zhao Jinkai Wang Jun Li Department of Rehabilitation Medicine Key Laboratory of Liver Disease of Guangdong ProvinceThe Third Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China Department of Biochemistry Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Department of Medical Informatics Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Center for Stem Cell Biology and Tissue Engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationSun Yat-sen UniversityGuangzhou 510080China Biosafety Level-3 Laboratory Life Sciences InstituteGuangxi Medical UniversityNanning 530020China Department of Obstetrics and Gynecology The First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China State Key Laboratory of Oncology in South China Cancer CenterSun Yat-sen UniversityGuangzhou 510080China RNA Biomedical Institute Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510080China

Serine/arginine-rich splicing factor 7(SRSF7),a known splicing factor,has been revealed to play oncogenic roles in multiple ***,the mechanisms underlying its oncogenic roles have not been well ***,based on N6-methyladenosine(m^(6)A)co-methylation network analysis across diverse cell lines,we find that the gene expression of SRSF7 is positively correlated with glioblastoma(GBM)cell-specific m^(6)A *** then indicate that SRSF7 is a novel m^(6)A regulator,which specifically facilitates the m^(6)A methylation near its binding sites on the mRNAs involved in cell proliferation and migration,through recruiting the methyltransferase ***,SRSF7 promotes the proliferation and migration of GBM cells largely dependent on the presence of the m^(6)A *** two m^(6)A sites on the mRNA for PDZ-binding kinase(PBK)are regulated by SRSF7 and partially mediate the effects of SRSF7 in GBM cells through recognition by insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2).Together,our discovery reveals a novel role of SRSF7 in regulating m^(6)A and validates the presence and functional importance of temporal-and spatial-specific regulation of m^(6)A mediated by RNA-binding proteins(RBPs).

关键词： m^(6)A Serine/arginine-rich splicing factor 7 Cell-specific regulation Glioblastoma PDZ-binding kinase

大黄提取物/聚丁二酸丁二醇酯复合材料的分子动力学模拟及抗菌性能

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高分子材料科学与工程 2019年第4期35卷 101-107,114页

作者：宋洁柯如媛张敏费贵强马晓燕陕西科技大学化学与化工学院陕西省轻化工助剂重点实验室陕西西安710021 西北工业大学理学院陕西西安710129

从大黄(Rheum palmatum L.)中提取染色抗菌有效成分,并与聚丁二酸丁二醇酯(PBS)复合,制备双功能性大黄提取物/聚丁二酸丁二醇酯(RPLE/PBS)复合材料。采用Materials Studio(MS)软件对复合材料的界面作用进行了分子动力学模拟,研究了RPLE... 详细信息

从大黄(Rheum palmatum L.)中提取染色抗菌有效成分,并与聚丁二酸丁二醇酯(PBS)复合,制备双功能性大黄提取物/聚丁二酸丁二醇酯(RPLE/PBS)复合材料。采用Materials Studio(MS)软件对复合材料的界面作用进行了分子动力学模拟,研究了RPLE与PBS界面作用对复合材料性能的影响。复合材料红外光谱显示大黄提取物与PBS产生了分子间相互作用。MS模拟说明大黄提取物中的-OH与PBS酯基作用形成了静电能、氢键2种非键合作用,从而使得复合材料的界面结合作用增强。复合材料的热性能较PBS显著提高,力学性能呈现先增大后减小的趋势,降解速率同样高于PBS,均说明了分子间非键合作用对性能的影响。随着大黄提取物添加量的增加,未与PBS发生相互作用的提取物使得复合材料的有效抗菌单元增加,抗菌作用更强。

关键词：大黄提取物聚丁二酸丁二醇酯复合材料分子动力学模拟界面相互作用

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川芎嗪对先心病肺动脉高压影响作用的研究

安徽医科大学学报 2005年第3期40卷 241-244页

作者：张飞周汝元安徽医科大学第一附属医院心血管外科合肥230022

目的探讨川芎嗪对先心病肺动脉高压的影响。方法选取先天性心脏病45例。其中中、重度肺动脉高压30例;肺动脉压正常15例。分为川芎嗪治疗组(A组,n=15)及对照组(B组,n=15)。肺动脉压正常者为空白对照组(C组,n=15)。围术期不同时点测定血浆... 详细信息

目的探讨川芎嗪对先心病肺动脉高压的影响。方法选取先天性心脏病45例。其中中、重度肺动脉高压30例;肺动脉压正常15例。分为川芎嗪治疗组(A组,n=15)及对照组(B组,n=15)。肺动脉压正常者为空白对照组(C组,n=15)。围术期不同时点测定血浆中ET1、NO、cAMP、cGMP含量,治疗组于用药前及术中测定肺动脉压。结果①A组及B组各时点血浆ET1含量明显高于C组,NO、cAMP、cGMP含量明显低于C组,差异均有显著性(P0.05)。A组术后72h血浆ET1的含量明显下降,cGMP含量明显升高,差异有显著性(P0.05)。结论川芎嗪降低血浆中ET1的含量,增加NO、cAMP及cGMP的含量,提示川芎嗪可降低肺动脉张力及肺动脉压力,减少围术期肺动脉高压危象的发生率,提高手术的成功率。

关键词：心脏缺损,先天性/中药疗法高血压,肺性/中药疗法川芎嗪/药理学