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基于皮尔逊相关系数的动态签名验证方法

仪器仪表学报 2022年第7期43卷 279-287页

作者：刘若男辛义忠李岩沈阳工业大学信息科学与工程学院沈阳110142 沈阳体育学院基础部沈阳110102

针对动态签名验证中存在的动态特征长度不等、动态签名验证方法较复杂以及识别率较低等问题,提出了一种基于皮尔逊相关系数的动态签名验证方法。首先通过划分原始特征区域,筛选并计算对应区域内的特征权重和,然后利用皮尔逊相关分析法... 详细信息

针对动态签名验证中存在的动态特征长度不等、动态签名验证方法较复杂以及识别率较低等问题,提出了一种基于皮尔逊相关系数的动态签名验证方法。首先通过划分原始特征区域,筛选并计算对应区域内的特征权重和,然后利用皮尔逊相关分析法计算各签名特征间的相关系数;再将皮尔逊相关系数作为新特征,分析真伪签名的皮尔逊相关系数分布情况;最后结合高斯密度函数模型,并通过设置个体判别阈值来进行签名验证。实验结果表明,真签名内的皮尔逊相关系数普遍高于真伪签名间的皮尔逊相关系数,且本方法在SVC和xLongSignDB数据集上均展现了较优的签名验证性能,其中xLongSignDB数据集上的误拒率和误识率分别为2.1%和1.7%。

关键词：皮尔逊相关系数动态签名验证特征权重高斯密度函数

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不同视觉反馈对笔倾斜输入性能的影响

计算机辅助设计与图形学学报 2017年第8期29卷 1525-1535页

作者：辛义忠刘若男李岩沈阳工业大学信息科学与工程学院沈阳110870 沈阳体育学院体育信息技术系沈阳110102

针对笔倾斜输入过程中存在遮挡、视角错觉和缺乏反馈等问题,分析了视觉反馈的增加对笔倾斜输入性能的影响.首先结合笔倾斜的特点设计了图形全跟踪、图形部分跟踪、图形无跟踪、数字全跟踪、数字部分跟踪和数字无跟踪6种不同的笔倾斜视... 详细信息

针对笔倾斜输入过程中存在遮挡、视角错觉和缺乏反馈等问题,分析了视觉反馈的增加对笔倾斜输入性能的影响.首先结合笔倾斜的特点设计了图形全跟踪、图形部分跟踪、图形无跟踪、数字全跟踪、数字部分跟踪和数字无跟踪6种不同的笔倾斜视觉反馈;然后通过对比实验考察了经过视觉反馈后,在直接和间接设备上完成不同角间距的目标选择任务时笔倾斜输入的性能.实验结果表明,视觉反馈可以有效地提升目标选择的速度,而跟踪机制可以有效地提高目标选择的准确度;图形反馈的速度快于数字反馈;全跟踪反馈的错误率低于部分跟踪反馈;在直接设备上完成目标选择任务速度快,但在间接设备上完成目标选择任务的准确度更高.最后给出了笔倾斜输入和视觉反馈的使用原则和建议,对笔式界面中的笔倾斜交互和视觉反馈设计具有指导意义.

关键词：笔式交互笔倾斜视觉反馈

新型动脉粥样硬化斑块示踪剂^(68)Ga-NOTA-CD44的制备与生物学评价

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中华核医学与分子影像杂志 2024年第2期44卷 104-108页

作者：王波李莉宇雪张楚欣鄢敏李慧玲茹慧宾武萍王若楠武志芳李思进山西医科大学第一医院核医学科、分子影像精准诊疗省部共建协同创新中心太原030001 山西医科大学医学影像学院太原030001

目的构建靶向透明质酸(HA)新型动脉粥样硬化(AS)示踪剂^(68)Ga-1,4,7-三氮杂环壬烷-1,4,7-三乙酸(NOTA)-CD44,并进行生物学评价及分子显像研究。方法选取小分子人重组CD44蛋白,在该蛋白羧基末端(C端)通过磺基化修饰后偶联双功能配体NOTA... 详细信息

目的构建靶向透明质酸(HA)新型动脉粥样硬化(AS)示踪剂^(68)Ga-1,4,7-三氮杂环壬烷-1,4,7-三乙酸(NOTA)-CD44,并进行生物学评价及分子显像研究。方法选取小分子人重组CD44蛋白,在该蛋白羧基末端(C端)通过磺基化修饰后偶联双功能配体NOTA,合成靶向HA的核素标记分子探针^(68)Ga-NOTA-CD44。研究探针的标记率、体外稳定性等生物学性质,并对3只AS斑块模型小鼠及3只正常C57BL/6小鼠行^(68)Ga-NOTA-CD44 microPET/CT显像及病理对照研究。结果合成的探针^(68)Ga-NOTA-CD44放化纯大于99%,比活度为62.22 MBq/nmol;探针在PBS中稳定性良好,放置3 h放化纯大于90%;探针经静脉注射后主要经肾代谢,在肝、肺及血液中代谢依次减低。AS模型小鼠microPET/CT显像示,该探针注射后60 min腹主动脉斑块处摄取较高,SUV_(max)与靶/本底比(TBR)_(max)分别为1.14±0.02及4.95±0.93,具有一定的AS侵蚀斑块靶向性,与病理结果一致。结论新型分子探针^(68)Ga-NOTA-CD44的制备简便且标记率高,具有较好的理化性质及体内生物学性质,靶向显示AS侵蚀斑块的灵敏度高,其分子影像在早期无创识别AS侵蚀斑块方面具有良好应用前景。

关键词：斑块,动脉粥样硬化抗原,CD44 同位素标记镓放射性同位素小鼠

A systematic identification of cold tolerance genes in peanut using yeast functional screening system

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Oil Crop Science 2023年第3期8卷 184-190页

作者： Xin Wang Yue Liu ruonan Yao Lei Ouyang Tingting Yu Liying yan Yuning Chen Dongxin Huai Zhihui Wang yanping Kang Qianqian Wang Huifang Jiang Yong Lei Boshou Liao Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural AffairsOil Crops Research Institute of the Chinese Academy of Agricultural SciencesWuhan430062PR China State Key Laboratory of Biocatalysis and Enzyme Engineering School of Life SciencesHubei UniversityWuhan430062PR China

Peanut(Arachis hypogaea L.)is a thermophilic crop,and low temperature leads to a significant reduction in annual *** a few cold tolerant germplasms or cultivars have been discovered and developed,molecular mechanisms governing peanut cold tolerance is poorly *** of keys genes involved in cold tolerance is the first step to address the underlying *** this study,we isolated and characterized 157 genes with potentials to confer cold tolerance in peanut by using a yeast functional screening ***(Gene ontology)and KEGG(Kyoto encyclopedia of genes and genomes)enrichment analysis of these genes revealed that ribosome and photosynthesis proteins might play essential roles in peanut cold *** results indicated that 60 cold tolerance candidate genes were significantly induced or depressed by low ***-PCR analysis demonstrated that several candidate genes could be also regulated by salt or drought *** overexpression of two UDP-glycosyltransferases(AhUGT2 and AhUGT268)in transgenic yeast cells could enhance their tolerance to multiple abiotic *** conclusion,this study advances our understanding of the mechanisms associated with the cold stress responses in peanut,and offers valuable gene resources for genetic improvement of abiotic stress tolerance in crops.

关键词： Peanut Yeast screening system Cold tolerance UDP-glycosyltransferase Abiotic stress

The inflammatory microenvironment and the urinary microbiome in the initiation and progression of bladder cancer

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Genes & Diseases 2021年第6期8卷 781-797页

作者： Xingxing Huang Ting Pan Lili yan Ting Jin ruonan Zhang Bi Chen Jiao Feng Ting Duan Yu Xiang Mingming Zhang Xiaying Chen Zuyi yang Wenzheng Zhang Xia Ding Tian Xie Xinbing Sui Department of Medical Oncology the Affiliated Hospital of Hangzhou Normal UniversityCollege of MedicineHangzhou Normal UniversityHangzhouZhejiang Province310015PR China College of Pharmacy School of MedicineHangzhou Normal UniversityHangzhouZhejiang Province311121PR China Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines Engineering Laboratory of Development and Application of Traditional Chinese MedicinesCollaborative Innovation Center of Traditional Chinese Medicines of Zhejiang ProvinceHangzhou Normal UniversityHangzhouZhejiang Province311121PR China School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing100029PR China

Accumulating evidence suggests that chronic inflammation may play a critical role in various malignancies,including bladder *** hypothesis stems in part from inflammatory cells observed in the urethral *** inflammation may drive neoplastic transformation and the progression of bladder cancer by activating a series of in-flammatory molecules and ***,it has been shown that the microbiome also plays an important role in the development and progression of bladder cancer,which can be mediated through the stimulation of chronic *** effect,the urinary microbiome can play a role in establishing the inflammatory urethral microenvironment that may facilitate the development and progression of bladder *** other words,chronic inflammation caused by the urinary microbiome may promote the initiation and progression of bladder ***,we provide a detailed and comprehensive account of the link between chronic inflammation,the microbiome and bladder ***,we highlight that targeting the urinary microbiome might enable the development of strategies for bladder cancer prevention and personalized treatment.

关键词： Bladder cancer Carcinogenesis Inflammatory microenvironment Pathogenesis Progression Urinary microbiome

An ATF24 peptide-functionalized β-elemene-nanostructured lipid carrier combined with cisplatin for bladder cancer treatment

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Cancer Biology & Medicine 2020年第3期17卷 676-692页

作者： Bingtao Zhai Peng Chen Wengang Wang Shuiping Liu Jiao Feng Ting Duan Yu Xiang ruonan Zhang Mingming Zhang Xuemeng Han Xiaying Chen Qiujie Li Guohua Li Ying Liu Xingxing Huang Wenzheng Zhang Ting Pan Lili yan Ting Jin Tian Xie Xinbing Sui Department of Medical Oncology the Affiliated Hospital of Hangzhou Normal UniversityCollege of MedicineHangzhou Normal UniversityHangzhou 310018China Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Provinee and Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province Hangzhou Normal UniversityHangzhou 310018China College of Pharmacy Chengdu University of Traditional Chinese MedicineChengdu 611137China Department of Medical Oncology Sir Run Run Shaw HospitalZhejiang UniversityHangzhou 310016China

Objective:In this study,we aimed to develop an amino-terminal fragment(ATF)peptide-targeted liposome carryingβ-elemene(ATF24-PEG-Lipo-β-E)for targeted delivery into urokinase plasminogen activator receptor-overexpressing bladder cancer cells combined with cisplatin(DDP)for bladder cancer ***:The liposomes were prepared by ethanol injection and high-pressure microjet *** liposomes were characterized,and the drug content,entrapment efficiency,andin vitro release were *** targeting efficiency was investigated using confocal microscopy,ultra-fast liquid chromatography,and an orthotopic bladder cancer *** effects of ATF24-PEG-Lipo-β-E combined with DDP on cell viability and proliferation were evaluated by a Cell Counting Kit-8(CCK-8)assay,a colony formation assay,and cell apoptosis and cell cycle *** anticancer effects were evaluated in a KU-19-19 bladder cancer xenograft ***:ATF24-PEG-Lipo-β-E had small and uniform sizes(~79 nm),high drug loading capacity(~5.24 mg/mL),high entrapment efficiency(98.37±0.95%),and exhibited sustained drug release ***24-PEG-Lipo-β-E had better targeting efficiency and higher cytotoxicity than polyethylene glycol(PEG)ylatedβ-elemene liposomes(PEG-Lipo-β-E).DDP,combined with ATF24-PEG-Lipo-β-E,exerted a synergistic effect on cellular apoptosis and cell arrest at the G2/M phase,and these effects were dependent on the caspase-dependent pathway and Cdc25C/Cdc2/cyclin B1 ***,thein vivo antitumor activity showed that the targeted liposomes effectively inhibited the growth of tumors,using the combined ***:The present study provided an effective strategy for the targeted delivery ofβ-elemene(β-E)to bladder cancer,and a combined strategy for bladder cancer treatment.

关键词： uPAR β-elemene active targeting liposome bladder cancer DDP

Functional Identification of the Stable Transfection C5aR Cell Line Molt-4

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Cellular & Molecular Immunology 2007年第6期4卷 461-465页

作者： Chunmei Zhang ruonan Xu Jianan Wang Gencheng Han Guojiang Chen Renxi Wang Huawei Wei Beifen Shen Yuanfang Ma yan Li Institute of Basic Medical Sciences Beijing China Laboratory of Cellular and Molecular Immunology Henan University Kaifeng China

The complement C5 anaphylatoxin receptor is a member of the seven transmembrane-spanning G protein-coupled receptor superfamily that signals through Gcxi and Gtz16. C5aR is mostly expressed on neutrophils, macrophages and endothelial cells. C5a and C5aR interaction plays an important role in numerous biological effects such as in vivo cytokine storm which results in inflammatory damage. Considering the limitation of collection of human peripheral blood neutrophils and their short half life, the stably transfected cell line for studying the biological effects of C5aR is needed. In this study, we transfected C5aR gene into Molt-4 cell line and examined the function of ectopic C5aR. Our results showed stable expression of the C5aR in Molt-4 cell line and their interaction with human C5a induced ERKI/2 phosphorylation, Ca＋＋ influx. This stable transfected cell line may provide a useful tool for studying signal pathways related to C5a and C5aR interplay and antibody development specific for C5aR. Cellular ＆ Molecular Immunology.

关键词： complement C5a C5aR Molt-4

Rice FLOURY ENDOSPERM 18 encodes a pentatricopeptide repeat protein required for 5′ processing of mitochondrial nad5 messenger RNA and endosperm development

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Journal of Integrative Plant Biology 2021年第5期63卷 834-847页

作者： Mingzhou Yu Mingming Wu Yulong Ren Yihua Wang Jingfang Li Cailin Lei Yinglun Sun Xiuhao Bao Hongming Wu Hang yang Tian Pan Yongfei Wang ruonan Jing Mengyuan yan Houda Zhang Lei Zhao Zhichao Zhao Xin Zhang Xiuping Guo Zhijun Cheng Bing yang Ling Jiang Jianmin Wan State Key Laboratory for Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Pentatricopeptide repeat(PPR) proteins, composing one of the largest protein families in plants,are involved in RNA binding and regulation of organelle RNA metabolism at the posttranscriptional level. Although several PPR proteins have been implicated in endosperm development in rice(Oryza sativa), the molecular functions of many PPRs remain obscure. Here, we identified a rice endosperm mutant named floury endosperm 18(flo18) with pleiotropic defects in both reproductive and vegetative ***-based cloning and complementation tests showed that FLO18 encodes a mitochondriontargeted P-type PPR protein with 15 PPR *** function was disrupted in the flo18 mutant, as evidenced by decreased assembly of Complex I in the mitochondrial electron transport chain and altered mitochondrial morphology. Loss of FLO18 function resulted in defective 5′-end processing of mitochondrial nad5 transcripts encoding subunit 5 of nicotinamide adenine dinucleotide hydrogenase. These results suggested that FLO18 is involved in 5′-end processing of nad5 messenger RNA and plays an important role in mitochondrial function and endosperm development.

关键词： aleurone layer endosperm development mitochondria PPR nad5,5′end processing

Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1

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Acta Pharmaceutica Sinica B 2021年第12期11卷 4045-4054页

作者： Na Kong Xiaying Chen Jiao Feng Ting Duan Shuiping Liu Xueni Sun Peng Chen Ting Pan Lili yan Ting Jin Yu Xiang Quan Gao Chengyong Wen Weirui Ma Wencheng Liu Mingming Zhang Zuyi yang Wengang Wang ruonan Zhang Bi Chen Tian Xie Xinbing Sui Wei Tao College of Pharmacy and Department of Medical Oncology the Affiliated Hospital of Hangzhou Normal UniversitySchool of MedicineHangzhou Normal UniversityHangzhou 311121China Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines Engineering Laboratory of Development and Application of Traditional Chinese MedicinesCollaborative Innovation Center of Traditional Chinese Medicines of Zhejiang ProvinceHangzhou Normal UniversityHangzhou 311121China Center for Nanomedicine and Department of Anesthesiology Brigham and Women’s HospitalHarvard Medical SchoolBostonMA 02115USA

Ferroptosis is a non-apoptotic regulated cell death caused by iron accumulation and subsequent lipid ***,the therapeutic role of ferroptosis on cancer is gaining increasing *** an active component in Scutellaria baicalensis Georgi with anticancer potential various cancer types;however,the effects of baicalein on bladder cancer and the underlying molecular mechanisms remain largely *** the study,we investigated the effect of baicalin on bladder cancer cells5637 and *** a result,we show baicalin exerted its anticancer activity by inducing apoptosis and cell death in bladder cancer ***,we for the first time demonstrate baicalin-induced ferroptotic cell death in vitro and in vivo,accompanied by reactive oxygen species(ROS) accumulation and intracellular chelate iron *** ferroptosis inhibitor deferoxamine but not necrostatin-1,chloroquine(CQ),N-acetyl-L-cysteine,L-glutathione reduced,or carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone(Z-VAD-FMK) rescued baicalin-induced cell death,indicating ferroptosis contributed to baicalin-induced cell ***,we show that ferritin heavy chain1(FTH1) was a key determinant for baicalin-induced *** of FTH1 abrogated the anticancer effects of baicalin in both 5637 and KU19-19 *** together,our data for the first time suggest that the natural product baicalin exerts its anticancer activity by inducing FTH1-dependent ferroptosis,which will hopefully provide a prospective compound for bladder cancer treatment.

关键词： Baicalin Ferroptosis Bladder cancer FTH1 Deferoxamine