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Biosynthesis and regulation of diterpenoids in medicinal plants

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Chinese Journal of Natural Medicines 2022年第10期20卷 761-772页

作者： REN Junze WU Yu HU Zhanpin chen ruibing ZHANG Lei Department of Pharmaceutical Botany School of PharmacyNaval Medical UniversityShanghai 200433China Institute of Interdisciplinary Integrative Medicine Research School of MedicineNantong UniversityNantong 226001China Biomedical Innovation R&D Center School of MedicineShanghai UniversityShanghai 20444China

Plant diterpenoids are widely distributed and abundant natural products with diverse structures and functions in nature,which have been commonly used in pharmaceutical,agricultural and industrial *** recent years,plant diterpenoids have attracted increasing attention,including their biosynthetic pathways,transcriptional regulatory networks,and biological ***,the biosynthetic pathways of diterpenoids are summarized in a modular ***,the regulatory network between diterpene biosynthesis and environmental factors is *** into diterpene metabolism may drive elucidation of complex active diterpene pathways and serve as a knowledge repository for metabolic engineering and cell factory construction.

关键词： Diterpenoid Pharmaceutical value Biosynthesis Regulation Medicinal plant

The ERF transcription factor LTF1 activates DIR1 to control stereoselective synthesis of antiviral lignans and stress defense in Isatis indigotica roots

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Acta Pharmaceutica Sinica B 2024年第1期14卷 405-420页

作者： ruibing chen Jian Yu Luyao Yu Liang Xiao Ying Xiao Junfeng chen Shouhong Gao Xianghui chen Qing Li Henan Zhang Wansheng chen Lei Zhang Department of Pharmaceutical Botany School of PharmacyNaval Medical UniversityShanghai 200433China Research and Development Center of Chinese Medicine Resources and Biotechnology Shanghai University of Traditional Chinese MedicineShanghai 201203China Department of Pharmacy Shanghai Changzheng HospitalNaval Medical UniversityShanghai 200003China School of Medicine Shanghai UniversityShanghai 200433China Institute of Edible Fungi Shanghai Academy of Agricultural SciencesNational Engineering Research Center of Edible FungiKey Laboratory of Edible Fungi Resources and Utilization(South)Ministry of AgricultureShanghai 201403China College of Life Sciences and Medicine Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang ProvinceZhejiang Sci-Tech UniversityHangzhou 310018China State Key Laboratory of Dao-di Herbs Beijing 100700China

Lignans are a powerful weapon for plants to resist stresses and have diverse bioactive functions to protect human *** the mechanisms of stereoselective biosynthesis and response to stresses of lignans is important for the guidance of plant ***,we identified the complete pathway to stereoselectively synthesize antiviral(-)-lariciresinol glucosides in Isatis indigotica roots,which consists of three-step sequential stereoselective enzymes DIR1/2,PLR,and ***1 was further identified as the key gene in respoJanuary 2024nse to stresses and was able to trigger stress defenses by mediating the elevation in lignan ***,the phytohormone-responsive ERF transcription factor LTF1 colocalized with DIR1 in the cell periphery of the vascular regions in mature roots and helped resist biotic and abiotic stresses by directly regulating the expression of *** systematic results suggest that DIR1 as the first common step of the lignan pathway cooperates with PLR and UGT71B2 to stereoselectively synthesize(-)-lariciresinol derived antiviral lignans in *** roots and is also a part of the LTF1-mediated regulatory network to resist *** conclusion,the LTF1-DIR1 module is an ideal engineering target to improve plant Defenses while increasing the content of valuable lignans in plants.

关键词： Lignans Stereoselective synthesis Stress resistance Dirigent protein ERF

Simultaneous determination of eight bioactive bakkenolides of Petasites tatewakianus Kitam by HPLC–UV

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Acta Pharmaceutica Sinica B 2013年第5期3卷 354-360页

作者： Jianming He Qin Wang Yan Wang ruibing chen Yuyang Zhang Meili Guo Department of Pharmacognosy School of PharmacySecond Military Medical UniversityShanghai 200433China

A high-performance liquid chromatography coupled with variable wavelength detection(HPLC–UV)was developed to evaluate the quality of Petasites tatewakianus Kitam through a simultaneous determination of eight bakkenolides:bakkenolide-L,bakkenolide-D,bakkenolide-B,bakkenolide-Ⅰa,bakkenolide-Ⅶa,bakkenolide-Ⅳa,bakkenolide-Ⅲa and *** a C18 analytical column,the eight analytes were efficiently separated using tetrahydrofuran–acetonitrile–water as the mobile phase in a constant *** limits of detection and limits of quantitation of the method ranged 0.42–2.56μg/mL and 1.22–8.40μg/mL,*** intra-and inter-day precisions of the method were all less than 1.83%.All the recoveries for the spiked analytes ranged 97.8%–102.4%.There were statistically significant differences among the contents of the eight bakkenolides in different parts and origins of ***(Po0.01).The content of total bakkenolides in rhizome was higher than that in other parts of the *** content of total bakkenolides in rhizome from Baishan was higher than those in other *** result suggested that rhizome may be the most valuable part of ***,and *** from Baishan may be the best plant *** results might serve as a sound foundation for further study and application of this plant.

关键词： HPLC–UV Bakkenolide Petasites tatewakianus Kitam Quantitative determination

TRICHOMEAND ARTEMISININ REGULATOR 1 Is Required for Trichome Development and Artemisinin Biosynthesis in Artemisia annua

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Molecular Plant 2015年第9期8卷 1396-1411页

作者： Hexin Tan Ling Xiao Shouhong Gao Qing Li Junfeng chen Ying Xiao Qian Ji ruibing chen Wansheng chen Lei Zhang Department of Pharmaceutical Botany School of Pharmacy Second Military Medical University Shanghai 200433 China Department of Pharmacy Changzheng Hospital Second Military Medical University Shanghai 200003 China These authors contributed equally to this article.

Trichomes, small protrusions on the surface of many plant species, can produce and store various secondary metabolic products. Artemisinin, the most famous and potent medicine for malaria, is synthesized, stored, and secreted by Artemisia annua trichomes. However, the molecular basis regulating the biosynthesis of artemisinin and the development of trichomes in A. annua remains poorly understood. Here, we report that an AP2 transcription factor, TRICHOME AND ARTEMISININ REGULATOR 1 （TAR1）, plays crucial roles in regulating the development of trichomes and the biosynthesis of artemisinin in A. annua. TAR1, which encodes a protein specially located in the nucleus, is mainly expressed in young leaves, flower buds, and some trichomes. In TAR1-RNAi lines, the morphology of trichomes and the composition of cuticular wax were altered, and the artemisinin content was dramatically reduced, which could be significantly increased by TAR1 oeverexpression. Expression levels of several key genes that are involved in artemisinin biosynthesis were altered when TAR1 was silenced or overexpressed. By the electrophoretic mobility shift, yeast one-hybrid and transient transformation β-glucuronidase assays, we showed that ADS and CYP71AV1, two key genes in the biosynthesis pathway of artemisinin, are likely the direct targets of TAR1. Taken together, our results indicate that TAR1 is a key component of the molecular network regulating trichome development and artemisinin biosynthesis in A. annua.

关键词： Artemisia annua artemisinin trichome AP2 transcription factor wax

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使用国产设备器材维修引进清梳联

棉纺织技术 2000年第4期28卷 43-44页

作者：陈瑞兵安徽省蚌埠天马纺织(集团)有限公司

我公司于1994年引进德国特吕茨施勒尔清梳联一套,其工艺流程如下:FA006型抓棉机(国产)—→MRO型吸铁杂装置—→LVSAB型高效凝棉器(附重物落杂)—→AFC型双轴流开棉机—→MM6型多仓混棉机—→CVT3型三辊开棉机—→DX型强力除尘器—→FBK... 详细信息

我公司于1994年引进德国特吕茨施勒尔清梳联一套,其工艺流程如下:FA006型抓棉机(国产)—→MRO型吸铁杂装置—→LVSAB型高效凝棉器(附重物落杂)—→AFC型双轴流开棉机—→MM6型多仓混棉机—→CVT3型三辊开棉机—→DX型强力除尘器—→FBK533型喂棉箱×8—→DK760型梳棉机×8

关键词：德国清梳联设备维修国产设备器材

Utilizing engineered extracellular vesicles as delivery vectors in the management of ischemic stroke:a special outlook on mitochondrial delivery

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Neural Regeneration Research 2025年第8期20卷 2181-2198页

作者： Jiali chen Yiyang Li Xingping Quan Jinfen chen Yan Han Li Yang Manfei Zhou Greta Seng Peng Mok ruibing Wang Yonghua Zhao Institute of Chinese Medical Sciences State Key Laboratory of Quality Research in Chinese MedicineUniversity of MacaoTaipaMacao Special Administrative RegionChina Department of Pharmacy Hunan Provincial People’s Hospitalthe First Affiliated Hospital of Hunan Normal UniversityChangshaHunan ProvinceChina Department of Electrical and Computer Engineering University of MacaoTaipaMacao Special Administrative RegionChina Department of Pharmaceutical Sciences Faculty of Health SciencesUniversity of MacaoTaipaMacao Special Administrative RegionChina

Ischemic stroke is a secondary cause of mortality worldwide,imposing considerable medical and economic burdens on *** vesicles,serving as natural nanocarriers for drug delivery,exhibit excellent biocompatibility in vivo and have significant advantages in the management of ischemic ***,the uncertain distribution and rapid clearance of extracellular vesicles impede their delivery *** utilizing membrane decoration or by encapsulating therapeutic cargo within extracellular vesicles,their delivery efficacy may be greatly ***,previous studies have indicated that microvesicles,a subset of large-sized extracellular vesicles,can transport mitochondria to neighboring cells,thereby aiding in the restoration of mitochondrial function post-ischemic *** extracellular vesicles have also demonstrated the capability to transfer mitochondrial components,such as proteins or deoxyribonucleic acid,or their sub-components,for extracellular vesicle-based ischemic stroke *** this review,we undertake a comparative analysis of the isolation techniques employed for extracellular vesicles and present an overview of the current dominant extracellular vesicle modification *** the complex facets of treating ischemic stroke,we also delineate various extracellular vesicle modification approaches which are suited to different facets of the treatment ***,given the burgeoning interest in mitochondrial delivery,we delved into the feasibility and existing research findings on the transportation of mitochondrial fractions or intact mitochondria through small extracellular vesicles and microvesicles to offer a fresh perspective on ischemic stroke therapy.

关键词： delivery engineering extracellular vesicles identification ischemic stroke isolation mitochondria targeting strategy therapeutic effects

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代谢酶与RNA相互作用的研究进展

中国生物工程杂志 2024年第2期44卷 124-133页

作者：张竹玉姜娜陈瑞冰天津大学药物科学与技术学院天津300072

细胞代谢重编程对维持细胞稳态、细胞生长与增殖等细胞过程发挥着重要作用,并广泛参与恶性转化等病理过程。随着高通量分子检测技术的发展,人们发现有些代谢酶不仅能通过催化细胞内各种生化反应参与细胞代谢调控,同时还能结合RNA分子。... 详细信息

细胞代谢重编程对维持细胞稳态、细胞生长与增殖等细胞过程发挥着重要作用,并广泛参与恶性转化等病理过程。随着高通量分子检测技术的发展,人们发现有些代谢酶不仅能通过催化细胞内各种生化反应参与细胞代谢调控,同时还能结合RNA分子。这些代谢酶不具备经典的RNA结合域。已有研究显示它们可能通过一种负反馈机制调控其结合mRNA的运输、稳定性或翻译,从而将基因表达调控与细胞代谢联系起来。除此之外,酶的代谢产物也可能参与RNA与代谢酶相互作用的调控。重点从近年来发现的具备RNA结合能力的代谢酶、代谢酶与RNA的相互作用方式、RNA结合蛋白的鉴定与验证、代谢调控机制以及这些代谢酶与RNA相互作用如何调控复杂的细胞活动和疾病的发生发展过程进行综述。

关键词： RNA结合蛋白代谢酶细胞代谢蛋白质组学质谱

结合亲和质谱与生物信息学分析构建TP53BP1的蛋白相互作用网络

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中国科学：生命科学 2018年第2期48卷 232-239页

作者：王浩王春晴陈瑞冰天津医科大学基础医学院遗传学系天津300070

蛋白质与蛋白质相互作用(PPIs)是两个或更多蛋白质分子之间通过静电作用、范德华力等建立的高度特异性的物理接触.细胞内的各种蛋白分子通过PPIs进行彼此之间的功能调节、信号通路的交互作用等,从而实现各种生物进程,而异常的PPIs也将... 详细信息

蛋白质与蛋白质相互作用(PPIs)是两个或更多蛋白质分子之间通过静电作用、范德华力等建立的高度特异性的物理接触.细胞内的各种蛋白分子通过PPIs进行彼此之间的功能调节、信号通路的交互作用等,从而实现各种生物进程,而异常的PPIs也将导致疾病的发生、发展,其中就包括肿瘤.因此围绕某些和疾病密切相关的关键蛋白构建其相互作用生物网络(interactome)将有助于更好地分析该关键蛋白在疾病中的作用和可能的分子机制.本研究针对抑癌基因p53结合蛋白1(TP53BP1),利用亲和质谱分析鉴定了15个潜在的TP53BP1相互作用蛋白.同时,结合PPI数据库检索构建了与TP53BP1相互作用的蛋白质网络,并对该网络中的蛋白进行了功能富集分析、通路分析,结果显示,TP53BP1相互作用蛋白主要富集在细胞周期、同源重组、错配修复等重要通路,该研究为深入解析TP53BP1的生物学功能及其在肿瘤中的作用奠定了基础.

关键词： TP53BP1 蛋白质相互作用功能富集分析亲和质谱分析

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塑料模具设计的塑件摆正方法探讨

模具制造 2022年第9期22卷 31-36,39页

作者：陈瑞兵东莞理工学校广东东莞523470

在进行塑料模具设计之前,需要先将塑件摆正,即保证塑件的脱模方向为+Z向,并根据塑件的尺寸、结构布局尺寸等确定X轴、Y轴的方向。通常模具设计师在设计塑件时,塑件的方向、角度、位置并不一定直接适用于模具设计。因此,模具设计师在得... 详细信息

在进行塑料模具设计之前,需要先将塑件摆正,即保证塑件的脱模方向为+Z向,并根据塑件的尺寸、结构布局尺寸等确定X轴、Y轴的方向。通常模具设计师在设计塑件时,塑件的方向、角度、位置并不一定直接适用于模具设计。因此,模具设计师在得到3D塑件模型后,需要先将塑件摆正,保证塑件相对于绝对坐标系处于一个正确的位置。详细介绍了几种常用的摆正方法,分享给大家参考。

关键词： UG Pro/E 模具设计塑件摆正

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