检索结果-南通市图书馆

同方期刊数据库

在线全文

学校读者我要写书评

暂无评论

Bone Research 2024年第1期12卷 122-136页

作者： Nan-Yu Zou Ran Liu Mei Huang Yu-rui Jiao Jie Wei Yangzi Jiang Wen-Zhen He Min Huang Yi-Li Xu Ling Liu Yu-Chen Sun Mi Yang Qi Guo Yan Huang Tian Su Ye Xiao Wei-Shan Wang Chao Zeng Guang-Hua Lei Xiang-hang Luo Chang-Jun Li Department of Endocrinology Endocrinology Research CenterXiangya Hospital of Central South UniversityChangshaHunan 410008China Department of Orthopaedics Xiangya Hospital of Central South UniversityChangshaHunan 410008China Department of Epidemiology and Health Statistics Xiangya School of Public HealthCentral South UniversityChangshaChina Hunan Key Laboratory of Joint Degeneration and Injury ChangshaHunan 410008China Key Laboratory of Aging-related Bone and Joint Diseases Prevention and Treatment Ministry of EducationXiangya HospitalCentral South UniversityChangshaChina School of Biomedical Sciences Institute for Tissue Engineering and Regenerative MedicineFaculty of MedicineThe Chinese University of Hong KongHong Kong SARChina Department of Orthopaedics The First Affiliated Hospital of Shihezi UniversityShiheziXinjiangChina National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China

Skeletal stem/progenitor cell(SSPC)senescence is a major cause of decreased bone regenerative potential with aging,but the causes of SSPC senescence remain *** this study,we revealed that macrophages in calluses secrete prosenescent factors,including grancalcin(GCA),during aging,which triggers SSPC senescence and impairs fracture *** injection of human rGCA in young mice induced SSPC senescence and delayed fracture *** deletion of Gca in monocytes/macrophages was sufficient to rejuvenate fracture repair in aged mice and alleviate SSPC ***,GCA binds to the plexin-B2 receptor and activates Arg2-mediated mitochondrial dysfunction,resulting in cellular *** of Plxnb2 in SSPCs impaired fracture *** of GCA-neutralizing antibody enhanced fracture healing in aged ***,our study revealed that senescent macrophages within calluses secrete GCA to trigger SSPC secondary senescence,and GCA neutralization represents a promising therapy for nonunion or delayed union in elderly individuals.

关键词： healing union senescence

Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human Mannan-Binding Lectin Protein-Conjugated Magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2024年第4期37卷 387-398页

作者： ZHAO Zi Jin CHEN Xiao Ping HUA Shao Wei LI Feng Yu ZHAO Meng XING Chen Hao WANG Jie TIAN Feng Yu Zhang rui Qing LYU Xiao Na HAN Zhi qiang WANG Yu Xin LI Hong Yi SHEN Xin Xin MA Xue Jun TIE Yan Qing Graduate School Hebei North UniversityZhangjiakou 075000HebeiChina Department of Clinical Laboratory Hebei General HospitalShijiazhuang 050051HebeiChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases NHC Key Laboratory of Medical Virology and Viral DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Department of Clinical Laboratory Pathology Diagnostic Center Shiyan General HospitalShiyan 442000HubeiChina Graduate School Hebei Medical University Shijiazhuang 050031HebeiChina

Objective Recombinase-aided polymerase chain reaction(RAP)is a sensitive,single-tube,two-stage nucleic acid amplification *** study aimed to develop an assay that can be used for the early diagnosis of three types of bacteremia caused by Staphylococcus aureus(SA),Pseudomonas aeruginosa(PA),and Acinetobacter baumannii(AB)in the bloodstream based on recombinant human mannanbinding lectin protein(M1 protein)-conjugated magnetic bead(M1 bead)enrichment of pathogens combined with *** Recombinant plasmids were used to evaluate the assay *** blood influenza bacteria were used for the specific *** and clinical plasma samples were enriched with M1 beads and then subjected to multiple recombinase-aided PCR(M-RAP)and quantitative PCR(qPCR)*** analysis was used to evaluate the consistency between the two *** The M-RAP method had sensitivity rates of 1,10,and 1 copies/μL for the detection of SA,PA,and AB plasmids,respectively,without cross-reaction to other bacterial *** M-RAP assay obtained results for<10 CFU/mL pathogens in the blood within 4 h,with higher sensitivity than qPCR.M-RAP and qPCR for SA,PA,and AB yielded Kappa values of 0.839,0.815,and 0.856,respectively(P<0.05).Conclusion An M-RAP assay for SA,PA,and AB in blood samples utilizing M1 bead enrichment has been developed and can be potentially used for the early detection of bacteremia.

关键词： Staphylococcus aureus Pseudomonas aeruginosa Acinetobacter baumannii Human Mannan-binding lectin protein Bloodstream infection Recombinase-aided PCR assay Multiple detection

在线全文

学校读者我要写书评

暂无评论

滑坡探测电磁仪配套数据采集软件设计与实现

Applied Geophysics 2024年第1期21卷 133-146,205页

作者：李斌许强刘天翔程强汤明高郑光雷航四川省公路规划勘察设计研究院有限公司四川成都610041 成都理工大学地质灾害防护与地质环境保护国家重点实验室四川成都610059

随着人类社会的高速发展和工程建设的激增,地质环境扰动日益显著,极端气候因素影响日益增多,地质灾害日益频繁发生,对人类的威胁和造成的损失也随之日益加剧。滑坡是其中最主要的地质灾害,因此,研究滑坡发育、发展、发生整个地质过程中... 详细信息

随着人类社会的高速发展和工程建设的激增,地质环境扰动日益显著,极端气候因素影响日益增多,地质灾害日益频繁发生,对人类的威胁和造成的损失也随之日益加剧。滑坡是其中最主要的地质灾害,因此,研究滑坡发育、发展、发生整个地质过程中伴随的物理现象,深入研究其信号特征,从而间接获取滑坡发展变化过程是地质灾害探测或监测的基础性研究,是值得深入探索的科学问题。但是专门用于滑坡探测的电磁仪器关键设计及具体实现的研究,特别是电磁仪器配套数据采集软件的设计及具体实现尚较缺乏。本研究以嵌入式计算机科学、软件工程、数字信号处理技术、地球物理、工程地质等多学科交叉的理论体系为基础,设计并实现了可用于滑坡探测或监测的仪器配套数据采集软件,拥有滑坡电磁法探测的嵌入式计算机软件自主知识产权,有助于科学探查滑坡地质灾害的动态发育、发展、发生。

关键词：滑坡大地电磁法工程地质嵌入式计算机数字信号处理软件设计

Genome editing in plants using the TnpB transposase system

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

aBIOTECH 2024年第2期5卷 225-230页

作者： Qi Li Yongqiang Wang Zhuoting Hou hang Zong Xuping Wang Yong EZhang Haoyi Wang Haitao Chen Wen Wang Kang Duan School of Ecology and Environment Northwestern Polytechnical UniversityXi’an 710129China Sanjie Institute of Forage Yangling 712100China Key Laboratory of Zoological Systematics and Evolution Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China

The widely used clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas)system is thought to have evolved from IS200/IS605 *** proteins,encoded by one type of IS200/IS605 transposon,are considered to be the evolutionary ancestors of Cas12 nucleases,which have been engineered to function as RNA-guided DNA endonucleases for genome editing in bacteria and human *** nucleases,which are smaller than Cas nucleases,have been engineered for use in genome editing in animal systems,but the feasibility of this approach in plants remained ***,we obtained stably transformed genome-edited mutants in rice(Oryza sativa)by adapting three recently identified TnpB genome editing vectors,encoding distinct TnpB nucleases(ISAam1,ISDra2,and ISYmu1),for use in plants,demonstrating that the hypercompact TnpB proteins can effectively edit plant ***2 and ISYmu1 precisely edited their target sequences,with no off-target mutations detected,showing that TnpB transposon nucleases are suitable for development into a new genome editing tool for *** modifications improving the genome-editing efficiency of the TnpB system will facilitate plant functional studies and breeding programs.

关键词： ISAam1 ISDra2 ISYmu1 Plant genome editing Rice TnpB transposon endonuclease

Applying an AIE type fluorescent probe in the detection of tetracycline antibiotics

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

分子科学学报 2024年第2期40卷 146-152页

作者： HU Bo ZHENG Luying WANG rui XU Kun ZHAO Sisi YU Zhan CHEN Qingyang Zhang hang College of Chemistry and Chemical Engineering Shenyang Normal UniversityShenyang 110034China

The typical representative TPB(tetraphenyl-1,3-butadiene)molecule with AIE effect was used as a fluorescent probe,and the specific recognition of tetracycline antibiotics was achieved through the comparison of the experimental results of the quenching of TPB by nine antibiotics of different types,which indicated that tetracycline antibiotics were found to quench TPB to the greatest extent,while the quenching of other antibiotics(***,aminoglycoside,sulfonamides,and quinolone)was not *** the quantitative relationship between the concentration of antibiotic solution and the effect of fluorescence quench was investigated by taking oxytetracycline among tetracycline antibiotics as an example,and the detection limit was ***,it was found that the UV absorption spectra of tetracycline hydrochloride and the fluorescence excitation spectra of TPB had high overlap,while the overlap between the UV absorption spectra of other antibiotics,such as sulfadimethoxine,and the fluorescence laser spectrum of TPB was low,which fulfilled the fluorescence internal filtering *** fluorescence probe has stable properties,low cost and strong anti-interference ability,which makes the method more valuable in the practical detection of *** study indicates great potent in the detection of antibiotics.

关键词： fluorescence detection technology tetraphenyl-1,3-butadiene tetracycline antibiotics

南酸枣叶原花青素的提取工艺优化及抗氧化活性研究

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国饲料 2024年第5期 51-56页

作者：张强渠淑洁戴航广西中医药大学药学院公共卫生管理学院广西南宁530299

本试验以南酸枣叶为原料,丙酮为溶剂,采用超声辅助提取法提取南酸枣叶中的原花青素,考察提取时间、料液比、温度和丙酮浓度四个因素对南酸枣叶原花青素得率的影响,结合正交试验优化南酸枣叶原花青素的提取工艺。最后对南酸枣叶原花青素... 详细信息

本试验以南酸枣叶为原料,丙酮为溶剂,采用超声辅助提取法提取南酸枣叶中的原花青素,考察提取时间、料液比、温度和丙酮浓度四个因素对南酸枣叶原花青素得率的影响,结合正交试验优化南酸枣叶原花青素的提取工艺。最后对南酸枣叶原花青素的抗氧化能力进行测定。结果表明:南酸枣叶原花青素的最佳提取工艺为超声时间8 min、料液比1:25、温度45℃、丙酮体积分数0.7。该条件下,原花青素得率为16.85 mg/g。另外,南酸枣叶原花青素有一定的抗氧化活性,其对DPPH和OH自由基的IC50分别为0.83 mg/mL和1.50 mg/mL。

关键词：南酸枣叶原花青素工艺优化抗氧化活性

反应堆安全壳整体泄漏率测量阶段持续时间优化研究

在线全文

学校读者我要写书评

暂无评论

工程设计学报 2024年第1期31卷 74-80页

作者：刘锐单强梁招瑞侯秦脉生态环境部核与辐射安全中心北京100082 中广核核电运营有限公司广东深圳518000 辽宁红沿河核电有限公司辽宁大连116000

为了降低反应堆安全壳整体泄漏率测量试验风险和使泄漏率测量结果更加保守和可信,基于空气稳定准则、函数曲率准则和数据分布准则,提出了一种适用于我国核电机组的安全壳整体泄漏率测量阶段持续时间的优化方法。结合法国电力集团的安全... 详细信息

为了降低反应堆安全壳整体泄漏率测量试验风险和使泄漏率测量结果更加保守和可信,基于空气稳定准则、函数曲率准则和数据分布准则,提出了一种适用于我国核电机组的安全壳整体泄漏率测量阶段持续时间的优化方法。结合法国电力集团的安全壳整体泄漏率测量阶段持续时间调整方法,明确了安全壳整体泄漏率测量试验结束的判定要求,并提出了增加安全壳内空气稳定判定准则和整体泄漏率测量终止判定准则的优化方法。以20组安全壳打压试验样本为例,计算了安全壳整体泄漏率测量阶段的持续时间。结果表明,共有17组样本满足优化条件,其中15组样本在16h内满足整体泄漏率测量终止判定准则,其余2组样本分别在19.25h和18.25h内满足全部的验收准则。所提出的优化方法能够缩短反应堆安全壳整体泄漏率测量阶段的持续时间,其比法国的方法更为保守、可信。

关键词：安全壳整体泄漏率测量阶段持续时间优化方法

贸易便利化与沿线城市产业结构升级——基于中欧班列的准自然实验

在线全文

学校读者我要写书评

暂无评论

价格月刊 2024年第5期 39-48页

作者：强国令白瑞新疆财经大学金融学院新疆乌鲁木齐830012

中欧班列的国际货运属性导致其对中国贸易便利化和技术创新的影响有别于一般国内铁路。基于2004—2021年中国地级市的数据,将中欧班列开通视为“准自然实验”,运用多期双重差分法探究贸易便利化对沿线城市产业结构升级的影响。结果显示... 详细信息

中欧班列的国际货运属性导致其对中国贸易便利化和技术创新的影响有别于一般国内铁路。基于2004—2021年中国地级市的数据,将中欧班列开通视为“准自然实验”,运用多期双重差分法探究贸易便利化对沿线城市产业结构升级的影响。结果显示:第一,中欧班列开通能够推动沿线城市产业结构朝着高级化方向发展,在进行三种稳健性检验后结果仍然显著。第二,技术创新、融资支持和数字智能三种机制能对开通城市的产业结构升级产生显著的正向影响。第三,中欧班列对沿线城市产业结构升级的推动作用主要存在于非中心城市。未来城市应利用贸易便利化这一有力“引擎”,通过提升融资便利与数字智能水平,充分发挥交通基础设施在促进创新要素流动中的作用,为沿线城市产业结构调整提供支撑。

关键词：产业结构升级贸易便利化中欧班列现代化产业体系双重差分

同方期刊数据库

邻域自适应粒子群算法求解地源热泵区域能源系统鲁棒优化调度问题

在线全文

学校读者我要写书评

暂无评论

控制理论与应用 2024年第6期41卷 1089-1100页

作者：吴亮红王维张红强贾睿湖南科技大学信息与电气工程学院湖南湘潭411201

针对地源热泵区域能源系统中冷热负荷和机组效能的不确定性,本文提出了一种考虑双重不确定性的鲁棒优化调度方法.首先,基于多面体不确定模型描述调度模型中的鲁棒变量.然后,针对建筑冷热负荷不确定性,采用对偶原理将双层优化模型等价为... 详细信息

针对地源热泵区域能源系统中冷热负荷和机组效能的不确定性,本文提出了一种考虑双重不确定性的鲁棒优化调度方法.首先,基于多面体不确定模型描述调度模型中的鲁棒变量.然后,针对建筑冷热负荷不确定性,采用对偶原理将双层优化模型等价为单层优化模型;对于机组效能不确定性,采用场景法进行分析.最后,采用多目标优化约束处理方法处理鲁棒优化调度模型中的约束条件.同时,为更加高效、准确求解所构建的优化调度模型,提出了一种邻域自适应粒子群优化算法(NAPSO).实验结果表明,在制冷和制热工况下,与经验运行策略相比,本文所提方法可分别减少7.22%和5.55%的系统运行成本,是一种解决地源热泵区域能源系统鲁棒优化调度的有效方法.

关键词：地源热泵鲁棒优化调度邻域自适应粒子群优化不确定性

维普期刊数据库博看期刊