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A novel method through solid phase extraction combined with gradient elution for concentration and separation of 66(ultra)trace persistent toxic pollutants in Antarctic waters

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Chinese Chemical Letters 2016年第3期27卷 405-411页

作者： li Zhang Guang-Shui Na Chun-Xiang He rui-jing li Hui Gao lin-Ke Ge Yan-Jie Wang Yao Yao School of Chemistry and Chemical Engineering Liaoning Normal University Key Laboratory for Ecological Environment in Coastal Areas (SOA) National Marine Environmental Monitoring Center

This study developed a method to perform the simultaneous concentration and selective separation of 66 （ultra） trace persistent toxic substances in Antarctic waters. The substances included 30 polychlorinated biphenyls, 17 organochlorine pesticides, 16 polycyclic aromatic hydrocarbons, 3 hexabromocyclododecanes. Solid phase extraction was performed using a C18 membrane and silica gel column. Gradient elution was conducted using organic solvents with different polarities; as a result, the efficiency of the C18 film is improved and the interferences from impurities and target compounds are eliminated. Extracts were subsequently analyzed through gas chromatography or liquid and gas chromatography coupled to tandem mass spectrometry. Method validation yielded the following values： recoveries of all target analytes in the Antarctic water ranged from 87.3% to 117.6% and reproducibility as percent relative standard deviation was lower than 5%. Quantification limits ranged from 0.004 μg L^-1 to 0.030 μg L^-1. The established method improved the recoveries and reduced the limits of detection. Results indicated the method exhibited good performance in the simultaneous concentration and selective separation of 66 （ultra） trace organic pollutants; Therefore, the proposed sample pretreatment can potentially eliminate the effects of various classes of impurities to some extent.

关键词： Polychlorinated biphenyls(PCBs) Organochlorine pesticides(OCPs) Polycyclic aromatic hydrocarbons(PAHs) Hexabromocyclododecanes(HBCDs) Solid phase extraction(SPE) Gradient elution

Construction of a lentiviral vector for RNA interference of human VIM gene and its silencing effect in pancreatic cancer cells

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The Chinese-German Journal of Clinical Oncology 2009年第3期8卷 145-149页

作者： Jianxin Jiang Ming Shen Renyi Qin rui Tian jing li Min Wang Department of Biliary-Pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Scienceand Technology Wuhan 430030 China

Objective： To construct a lentiviral expression vector for RNA interference （RNAi） of human VIM gene; and assess its gene silencing effect in pancreatic cancer cell line Panc-1. Methods： Three pairs of human VIM gene short hairpin RNA（shRNA） sequences were designed using a software available on-line and one pair came from document. After synthesis and annealing, four double-stranded oligonucleotides （dsOligo） were cloned into the pGCL-GFP/U6 plasmid, which were subsequently confirmed by polymerase chain reaction （PCR） and DNA sequencing analysis. Real-time PCR and Westemblotting were used to screen the effective pGCL-GFP-shRNA plasmid in 293T cells, then the most effective one was packed into the recombinant lentivirus Lv-VIM-shRNA with lentiviral packing materials pHelper 1.0 and pHelper 2.0 in 293T cells. The titer of lentivirus was determined by hole-by-dilution titer assay. The silencing effect of Lv-VIM-shRNA in Panc-1 calls were validated by real-time PCR and Western-blotting. Results： An effective Lv-VIM-shRNA was successfully constructed. The titer of lentivirus was determined on 2× 10^9TU/mL. The expressions of VIM mRNA and vimentin were down-regluated in the Panc-1 cells infected with Lv-VIM-shRNA. Conclusion： An effective Lv-VIM-shRNA could inhibit the expression of VIM gene in Panc-1 cells in vitro, which provides a tool for investigating the role of VIM gene in the signaling pathway involved in tumorigenesis and progression of pancreatic cancer and searching new therapeutic targets.

关键词： vimentin RNA interference lentivirus pancreatic carcinoma

Increase in the variability of terrestrial carbon uptake in response to enhanced future ENSO modulation

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 32-38页

作者： Younong li li Dan jing Peng Qidong Yang Fuqiang Yang Key Laboratory of Regional Climate-Environment for Temperate East Asia Institute of Atmospheric PhysicsChinese Academy of SciencesBeijingChina Key Laboratory of Atmospheric Environment and Processes in the Boundary Layer over the Low-Latitude Plateau Region Department of Atmospheric ScienceYunnan UniversityKunmingChina

El Niño-Southern Oscillation(ENSO)is a major driver of climate change in middle and low latitudes and thus strongly influences the terrestrial carbon cycle through land-air *** the ENSO modulation and carbon flux variability are projected to increase in the future,but their connection still needs further *** investigate the impact of future ENSO modulation on carbon flux variability,this study used 10 CMIP6 earth system models to analyze ENSO modulation and carbon flux variability in middle and low latitudes,and their relationship,under different scenarios simulated by CMIP6 *** results show a high consistency in the simulations,with both ENSO modulation and carbon flux variability showing an increasing trend in the *** higher the emissions scenario,especially SSP5-8.5 compared to SSP2-4.5,the greater the increase in *** flux variability in the middle and low latitudes under SSP2-4.5 increases by 30.9%compared to historical levels during 1951-2000,while under SSP5-8.5 it increases by 58.2%.Further analysis suggests that ENSO influences mid-and low-latitude carbon flux variability primarily through *** occurrence may potentially be attributed to the increased responsiveness of gross primary productivity towards regional temperature fluctuations,combined with the intensified influence of ENSO on land surface temperatures.

关键词： Carbon variability ENSO modulation CMIP6 models

山东滨海盐渍区植物群落与土壤化学因子的关系

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应用生态学报 2018年第11期29卷 3521-3529页

作者：尹德洁张洁荆瑞董丽国家花卉工程技术研究中心/城乡生态环境北京实验室/北京林业大学园林学院北京100083

在山东滨海盐渍区选取81个样方进行植物群落调查,对土壤化学性质进行分析,研究耐盐植物分布与土壤pH、可溶性盐总量、全氮、全磷、有机质、K^+、Na^+、Ca^(2+)、Mg^(2+)、Cl^-、SO_4^(2-)和HCO_3^-12个土壤化学因子之间的关系.结果表明... 详细信息

在山东滨海盐渍区选取81个样方进行植物群落调查,对土壤化学性质进行分析,研究耐盐植物分布与土壤pH、可溶性盐总量、全氮、全磷、有机质、K^+、Na^+、Ca^(2+)、Mg^(2+)、Cl^-、SO_4^(2-)和HCO_3^-12个土壤化学因子之间的关系.结果表明:共记录耐盐植物30科67属85种,排名前三的优势科为菊科、禾本科、藜科.群落类型分为4类,分别是芦苇-白茅+茵陈蒿群落、盐地碱蓬+芦苇-茵陈蒿+地肤群落、芦苇-香蒲+罗布麻群落和芦苇-罗布麻群落.CCA排序表明,土壤可溶性盐总量和水溶性离子含量是影响该区域耐盐植被分布的关键土壤化学因子,其次是土壤全氮含量.优势种为芦苇,同时伴生白茅和茵陈蒿的群落在4个群落类型中具有较丰富的物种多样性,所蕴含的耐盐植物资源丰富.群落的物种多样性与土壤可溶性盐总量和水溶性盐离子含量呈显著负相关,高盐分土壤会降低群落的物种多样性指数,影响群落的物种多样性.

关键词：滨海盐渍区土壤化学因子空间异质性耐盐植物

Relationship between parenting stress and behavioral and emotional problems in preschool children: A mediation effect analysis

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World Journal of Psychiatry 2025年第1期15卷 128-136页

作者： Zhi-Wei Fu Yue-jing li Ran Yu rui-Qing Guo li-Xia Gao Sheng-Xia Zhao Department of Children's Health Shijiazhuang Maternity and Child Healthcare HospitalShijiazhuang 050000Hebei ProvinceChina

BACKGROUND Emotional reactions,such as anxiety,irritability,and aggressive behavior,have attracted clinical attention as behavioral and emotional problems in preschool-age *** To investigate the current status of family rearing,parental stress,and behavioral and emotional problems of preschool children and to analyze the mediating effect of the current status of family rearing on parental stress and behavioral/emo-tional *** We use convenience sampling to select 258 preschool children in the physical examination center of our hospital from October 2021 to September *** children and their parents were evaluated using a questionnaire ***'s correlation was used to analyze the correlation between child behavioral and emotional problems and parental stress and family rearing,and the structural equation model was constructed to test the mediating *** The score for behavioral/emotional problems of 258 preschool children was(27.54±3.63),the score for parental stress was(87.64±11.34),and the score for parental family rearing was(31.54±5.24).There was a positive correlation between the behavioral and emotional problems of the children and the“hostile/mandatory”parenting style;meanwhile,showed a negative correlation with the“support/participation”parenting style(all Plinical medical staff should provide correct and reasonable parenting advice to their parents to promote the mental health of preschool-age children.

关键词： Preschool children Parenting stress Children's behavioral and emotional problems Family rearing Mediating effect

4例EB病毒感染相关急性肝功能衰竭患儿的临床特点分析

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中国当代儿科杂志 2018年第12期20卷 1030-1033页

作者：刘瑞海李晶曲妮燕李自普青岛市妇女儿童医院重症医学中心山东青岛266034

4例EB病毒感染相关急性肝功能衰竭患儿中男2例、女2例,年龄10(8.5～44)个月。3例诊断为传染性单核细胞增多症(IM),其中2例符合噬血细胞淋巴组织细胞增生症(HLH)诊断标准;1例诊断为EBV既往感染。4例患儿的血EBV-DNA载量均阳性。4例患儿... 详细信息

4例EB病毒感染相关急性肝功能衰竭患儿中男2例、女2例,年龄10(8.5～44)个月。3例诊断为传染性单核细胞增多症(IM),其中2例符合噬血细胞淋巴组织细胞增生症(HLH)诊断标准;1例诊断为EBV既往感染。4例患儿的血EBV-DNA载量均阳性。4例患儿入院时均有发热、肝大、黄疸,3例患儿具有脾大、腹水或者呕吐症状,2例患儿有颈淋巴结大、皮疹或胸水,1例患儿出现消化道出血或者2期肝性脑病。4例患儿的异型淋巴细胞计数均<10%,仅1例出现白细胞升高和血小板减少;4例患儿的转氨酶10～100倍升高、以直接胆红素为主的总胆红素3～5倍升高、乳酸脱氢酶数10倍升高、凝血酶原时间显著延长。4例患儿均静脉给予阿昔洛韦或更昔洛韦抗病毒、促肝细胞生长因子促进肝细胞生长以及激素减轻炎症反应等治疗;2例加用血浆置换治疗,其中1例联合连续性静脉-静脉血液透析滤过治疗;2例HLH患儿按照HLH2004方案化疗。3例存活,1例HLH因多脏器功能衰竭死亡。EB病毒感染可以引起儿童急性肝功能衰竭,早期给予包括血液净化治疗在内的综合治疗手段可能对预后有益。

关键词：急性肝功能衰竭病毒性肝炎 EB病毒儿童

An efficient quantum proactive incremental learning algorithm

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 49-57页

作者： lingxiao li jing li Yanqi Song Sujuan Qin Qiaoyan Wen Fei Gao State Key Laboratory of Networking and Switching Technology Beijing University of Posts and Telecommunications School of Cyberspace Security Beijing University of Posts and Telecommunications

In scenarios where a large amount of data needs to be learned, incremental learning can make full use of old knowledge, significantly reduce the computational cost of the overall learning process, and maintain high performance. In this paper, taking the MaxCut problem as our example, we introduce the idea of incremental learning into quantum computing, and propose a Quantum Proactive Incremental Learning algorithm(QPIL). Instead of a one-off training of quantum circuit, QPIL contains a multi-phase training on gradually-increased subgraphs of all vertices, proactively reducing large-scale problems to smaller ones to solve in steps, providing an efficient solution for MaxCut. Specifically, some vertices and corresponding edges are randomly selected for training to obtain optimized parameters of the quantum circuit at first. Then, in each incremental phase, the remaining vertices and corresponding edges are gradually added and the parameters obtained from the previous phase are reused in the parameter initialization of the current phase. We perform experiments on 120 different small-scale graphs, and it shows that QPIL performs superior to prevalent quantum and classical baselines in terms of approximation ratio(AR), time cost, anti-forgetting, and solving stability. In particular, QPIL's AR surpasses 20% of mainstream quantum baselines, and the time cost is less than 1/5 of them. The idea of QPIL is expected to inspire efficient and high-quality solutions in large-scale Max Cut and other combinatorial optimization problems.

关键词： variational quantum algorithm incremental learning multi-phase training MaxCut quantum computing

Unraveling post-growth mechanisms of monolayer CsPbBr3 nanocubes:Laser-enhanced transformations and cathodoluminescence-electron microscopy correlations

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Journal of Energy Chemistry 2025年第1期100卷 146-156页

作者： Mingxing li Xiaoge Wang Xiaofan Cao Zhiqun He Chunjun liang Mingxing Chen jing Ju Fangtian You Key Laboratory of Luminescence and Optical Information Ministry of Education Institute of Optoelectronic Technology Beijing Jiaotong University Beijing National Laboratory for Molecular Sciences (BNLMS) College of Chemistry and Molecular Engineering Peking University

Lead-halide perovskite nanoparticles（LHP NPs） are highly promising materials for next-generation displays and solid-state lighting due to their exceptional optical properties. However, their inherent instability presents a significant challenge. Recent advances have demonstrated that optoelectronic devices based on monolayer nanoparticle films exhibit both high luminescence efficiency and long-term *** research demonstrates that mobility limitations and anisotropic alignments in CsPbBr3nanocube monolayer films are key to their stabilization, hindering spontaneous growth through face-to-face fusion and resulting in the formation of connecting necks in a diagonal direction. Introducing laser irradiation confirmed this by significantly accelerating nanocubes growth, increasing mobility, and enhancing local structural ordering, leading to larger and more regularly shaped nanosheets. Fourier transform infrared spectroscopy and energy dispersive spectroscopy line-scan analyses indicated that laser irradiation did not disrupt the ligand structure. Transmission electron microscopy and correlative cathodoluminescence electron microscopy revealed the effects of post-growth and heterogeneous structures, including enhanced luminescence and inhomogeneous intensity in the nanosheets. These findings deepen the understanding of the post-growth mechanism of monolayer nanoparticles and the structure-emission correlation and highlight the unique role of laser irradiation in directing the formation of well-defined and regular nanostructures.

关键词： Post-growth mechanism Heterogeneous structure Single-particle spectroscopy Laser irradiation

Repetitive traumatic brain injury–induced complement C1–related inflammation impairs long-term hippocampal neurogenesis

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Neural Regeneration Research 2025年第3期20卷 821-835页

作者： jing Wang Bing Zhang Lanfang li Xiaomei Tang Jinyu Zeng Yige Song Chao Xu Kai Zhao Guoqiang liu Youming Lu Xinyan li Kai Shu Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Neurosurgery Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Pathophysiology School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Institute for Brain Research Wuhan Center of Brain ScienceHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Graduate Student Chongqing Medical UniversityChongqingChina Department of Basic Medicine School of Medical ScienceHubei University for NationalitiesEnshiHubei ProvinceChina Department of Anatomy School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Repetitive traumatic brain injury impacts adult neurogenesis in the hippocampal dentate gyrus,leading to long-term cognitive ***,the mechanism underlying this neurogenesis impairment remains *** this study,we established a male mouse model of repetitive traumatic brain injury and performed long-term evaluation of neurogenesis of the hippocampal dentate gyrus after repetitive traumatic brain *** results showed that repetitive traumatic brain injury inhibited neural stem cell proliferation and development,delayed neuronal maturation,and reduced the complexity of neuronal dendrites and *** with repetitive traumatic brain injuryalso showed deficits in spatial memory ***,following repetitive traumatic brain injury,neuroinflammation was enhanced in the neurogenesis microenvironment where C1q levels were increased,C1q binding protein levels were decreased,and canonical Wnt/β-catenin signaling was *** inhibitor of C1 reversed the long-term impairment of neurogenesis induced by repetitive traumatic brain injury and improved neurological *** findings suggest that repetitive traumatic brain injury–induced C1-related inflammation impairs long-term neurogenesis in the dentate gyrus and contributes to spatial memory retrieval dysfunction.

关键词： complement C1 dendrite dentate gyrus hippocampus neural stem cell neurogenesis neuroinflammation neurological function neuron traumatic brain injury