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Spatial analysis of the osteoarthritis microenvironment: techniques, insights, and applications

Bone Research 2024年第1期12卷 1-19页

作者： Xiwei Fan Antonia Rujia Sun Reuben S.E.Young Isaac O.Afara Brett R.Hamilton Louis Jun Ye Ong ross crawford Indira Prasadam Centre for Biomedical Technologies Queensland University of TechnologyBrisbaneQLDAustralia School of Mechanical Medical&Process EngineeringQueensland University of TechnologyBrisbaneQLDAustralia Central Analytical Research Facility Queensland University of TechnologyBrisbaneQLDAustralia Molecular Horizons University of WollongongWollongongNSWAustralia Department of Technical Physics University of Eastern FinlandKuopioFinland School of Electrical Engineering and Computer Science Faculty of EngineeringArchitecture and Information TechnologyUniversity of QueenslandBrisbaneQLDAustralia Centre for Microscopy and Microanalysis University of QueenslandBrisbaneQLDAustralia The Prince Charles Hospital BrisbaneQLDAustralia

Osteoarthritis(OA)is a debilitating degenerative disease affecting multiple joint tissues,including cartilage,bone,synovium,and adipose *** presents diverse clinical phenotypes and distinct molecular endotypes,including inflammatory,metabolic,mechanical,genetic,and synovial ***,innovative technologies are needed to support the development of effective diagnostic and precision therapeutic *** analysis of bulk OA tissue extracts has limitations due to technical constraints,causing challenges in the differentiation between various physiological and pathological phenotypes in joint *** issue has led to standardization difficulties and hindered the success of clinical *** insights into the spatial variations of the cellular and molecular structures in OA tissues,encompassing DNA,RNA,metabolites,and proteins,as well as their chemical properties,elemental composition,and mechanical attributes,can contribute to a more comprehensive understanding of the disease *** resolved biology enables biologists to investigate cells within the context of their tissue microenvironment,providing a more holistic view of cellular *** advances in innovative spatial biology techniques now allow intact tissue sections to be examined using various-omics lenses,such as genomics,transcriptomics,proteomics,and metabolomics,with spatial *** fusion of approaches provides researchers with critical insights into the molecular composition and functions of the cells and tissues at precise spatial ***,advanced imaging techniques,including high-resolution microscopy,hyperspectral imaging,and mass spectrometry imaging,enable the visualization and analysis of the spatial distribution of biomolecules,cells,and *** these molecular imaging outputs to conventional tissue histology can facilitate a more comprehensive characterization of disease *** review summarizes the rec

关键词： insight Spatial enable

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Clonal Characterization of Bone Marrow Derived Stem Cells and Their Application for Bone Regeneration

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International Journal of Oral Science 2010年第3期2卷 127-135页

作者： Shobha Mareddy ross crawford Institute of Health and Biomedical Innovation Queensland University of Technology

Tissue engineering allows the design of functionally active cells within supportive bio-scaffolds to promote the development of new tissues such as cartilage and bone for the restoration of pathologically altered tissues. However, all bone tissue engineering applications are limited by a shortage of stem cells. The adult bone marrow stroma contains a subset of nonhematopoietic cells referred to as bone marrow mesenchymal stem cells （BMSCs）. BMSCs are of interest because they are easily isolated from a small aspirate of bone marrow and readily generate singlecell-derived colonies. These cells have the capacity to undergo extensive replication in an undifferentiated state ex vivo. In addition, BMSCs have the potential to develop either in vitro or in vivo into distinct mesenchymal tissues,including bone, cartilage, fat, tendon, muscle, and marrow stroma. Thus, BMSCs are an attractive cell source for tissue engineering approaches. However, BMSCs are not homogeneous and the quantity of stem cells decreases in the bone marrow in aged population. A sequential loss of lineage differentiation potential has been found in the mixed culture of bone marrow stromal cells due to a heterogenous popu- lation. Therefore, a number of studies have proposed that homogenous bone marrow stem cells can be generated from clonal culture of bone marrow cells and that BMSC clones have the greatest potential for the application of bone regeneration in vivo.

关键词： bone morrow stem cell meserichymal clonalculture regenerative medicine differentiation

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Arthroscopic scene segmentation using multispectral reconstructed frames and deep learning

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Intelligent Medicine 2023年第4期3卷 243-251页

作者： Shahnewaz Ali ross crawford Ajay K.Pandey School of Electrical Engineering and Robotics Faculty of EngineeringQueensland University of TechnologyGardens PointBrisbaneQLD 4000Australia

Background Knee arthroscopy is one of the most complex minimally invasive surgeries,and it is routinelyperformed to treat a range of ailments and injuries to the knee *** complex ergonomic design imposesvisualization and navigation constraints,consequently leading to unintended tissue damage and a steep learningcurve before surgeons gain *** lack of robust visual texture and landmark frame features furtherlimits the success of image-guided approaches to knee arthroscopy Feature-and texture-less tissue structures ofknee anatomy,lighting conditions,noise,blur,debris,lack of accurate ground-truth label,tissue degeneration,and injury make semantic segmentation an extremely challenging *** address this complex research problem,this study reported the utility of reconstructed surface reflectance as a viable piece of information that could beused with cutting-edge deep learning technique to achieve highly accurate segmented *** We proposed an intraoperative,two-tier deep learning method that makes full use of tissue reflectanceinformation present within an RGB frame to segment texture-less images into multiple tissue types from kneearthroscopy video *** study included several cadaver knees experiments at the Medical and EngineeringResearch Facility,located within the Prince Charles Hospital campus,Brisbane *** were collectedfrom a total of five cadaver knees,three were males and one from a *** age range of the donors was 56–93 ***-related tissue degeneration and some anterior cruciate ligament injury were observed in mostcadaver *** arthroscopic image dataset was created and subsequently labeled by clinical *** also included validation of a prototype stereo arthroscope,along with conventional arthroscope,to attainlarger field of view and stereo *** reconstructed surface reflectance from camera responses that exhibiteddistinct spatial features at different wavelengths ranging from 3

关键词： Knee arthroscopy Segmentation Multispectral reconstructed Deep learning

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Dietary saturated fatty acid consumption and cholesterol level in osteoarthritis development

Dietary saturated fatty acid consumption and cholesterol lev...

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中华口腔医学会第十五次全国颞下颌关节病学及牙合学学术研...

作者： Indira Prasadam Sunderajhan Sekar Lindsay Brown Sunil Panchal ross crawford Yin Xiao Bone Group Institute of Health and Biomedical Innovation Queensland University of TechnologyKelvin Grove Campus Orthopaedic department Prince Charles Hospital School of Health University of Southern Queensland

A growing body of evidence suggests that osteoarthritis (OA) is not simply a disease related to aging or mechanical stress of joints but rather a"metabolic disorder"in which various interrelated lipid and metabolic mediators contribute to the initiation and progression of the disease process. One such metabolic risk factor could be high cholesterol

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加压植骨技术在先天性髋关节发育不良患者全髋置换术中修复髋臼缺损的应用

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中国现代手术学杂志 2013年第4期17卷 252-255页

作者：毛新展赵耀超 Sujoy Roychowdhury ross W crawford 中南大学湘雅二医院骨科长沙410011 昆士兰查尔斯王子医院

目的介绍加压植骨技术在先天性髋关节发育不良（congenital hip dysplasia，CDH）全髋关节置换术中修复髋臼缺损的应用，并对其临床早期效果进行评估。方法2012年8月～2013年5月我院对15例（15髋）CDH患者施行该术式。对患者手术前后X... 详细信息

目的介绍加压植骨技术在先天性髋关节发育不良（congenital hip dysplasia，CDH）全髋关节置换术中修复髋臼缺损的应用，并对其临床早期效果进行评估。方法2012年8月～2013年5月我院对15例（15髋）CDH患者施行该术式。对患者手术前后X线结果进行分析，应用Harris评分评价手术前后髋关节功能。结果本组患者术前患髋Harris评分平均为（45．2±10．6）分，术后3月随访提高到（81．1±16．5）分，术后未出现感染、脱位等早期并发症。结论通过联合应用加压植骨、钛网固定及骨水泥加压技术，患者髋臼形态得以重建，术后恢复良好。多种技术的联合应用在CDH患者全髋置换术中具有重要意义。

关键词：髋脱位,先天性加压植骨骨水泥关节成形术,置换,髋

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用含TGF-和OP-1的培养基诱导骨髓基质干细胞的双相分化修复骨软骨缺损

用含TGF-和OP-1的培养基诱导骨髓基质干细胞的双相分化修复骨软骨...

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第七次全国颞下颌关节病学及（牙合）学研讨会暨《颞下颌关节紊乱病及口颌面疼痛的基础与临床进展》国家级继续教育学习班

作者：李健龙星 Shobha Mareddy Dawn Meifang Tan ross crawford 武汉大学口腔医学院昆士兰科技大学健康和生物医学研究院

临床上骨关节炎的患者关节区会形成软骨和软骨下骨的缺损,形成疼痛并导致患者的运动障碍。为重建骨软骨缺损,拟使用组织工程的方法对骨髓基质干细胞(BMSCs)在不同载体材料但同一培养基系统内进行双相分化的诱导;培养基系统含地塞米松和...

临床上骨关节炎的患者关节区会形成软骨和软骨下骨的缺损,形成疼痛并导致患者的运动障碍。为重建骨软骨缺损,拟使用组织工程的方法对骨髓基质干细胞(BMSCs)在不同载体材料但同一培养基系统内进行双相分化的诱导;培养基系统含地塞米松和维生素C等可溶性物质,作为诱导成软骨和成骨形成的基础培养基(DCM)。DCM辅以能诱导成骨和成软骨的特定生长因长,在二维培养和三维培养系统内检测BMSCs形成骨和软骨的能力。TGF-β3被加入DCM,BMSCs形成软骨样细胞,表达糖胺多糖和Ⅱ型胶原;而加入OP-1后,BMSCs发生成骨样变化,表达出明显增高的骨桥素和骨钙素蛋白。在三维支架培养系统内,相应的成软骨和成骨分化的标志物的表达较在二维培养系统有显著增加。这些结果为将来形成双相复合物打下良好的理论基础,应用于治疗骨软骨全厚度缺损的修复。

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