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Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli

Asian Pacific Journal of Tropical Medicine 2022年第2期15卷 71-78页

作者： Guilherme S.Machado rosane l.lopes Simone S.C.Oliveira Simone P.C.Freitas Jacenir R.Santos-Mallet Andrél.S.Santos Denise Feder Suzete A.O.Gomes Programa de Pós-Graduação em Ciências e Biotecnologia Instituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil laboratório de Biodiversidade de Insetos e Patógenos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes Departamento de Microbiologia GeralInstituto de Microbiologia Paulo de GóesUniversidade Federal do Rio de JaneiroRJBrazil Fundação Oswaldo Cruz Piauí TeresinaPIBrazil laboratório Interdisciplinar de Vigilância Entomológica em Díptera e Hemíptera Instituto Oswaldo CruzFundação Oswaldo CruzRio de JaneiroRJBrazil Universidade Iguaçu Nova IguaçuRJBrazil laboratório de Biologia de Insetos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil

Objective:To investigate the morphological structure of ovarian follicular cells and biochemical parameters of both ovaries and fat bodies(sites of vitellogenesis)from Rhodnius(R.)prolixus infected with Trypanosoma(T.)***:Adult virgin females of *** were fed upon a membrane apparatus containing heat-inactivated citrated rabbit blood and a suspension of *** epimastigotes(Macias strain).Females from the control group and all the males received parasite-free *** electron microscopy was used to reveal the morphological aspects of ovarian follicle cells in both control and parasite-infected *** profile,proteolytic activities and Western blotting analyses were performed in either ovary or fat body samples of control and parasite-infected ***:According to the ultrastructural data,*** infection elicited a degeneration process in the ovarian follicular cells of *** assays indicated a reduction in the activity of aspartic peptidases in the ovary and fat body from parasite-infected group,while a significant increase in the cysteine peptidase activity was measured in both insect ***,immunoblotting revealed that vitellogenin was overexpressed in the ovary of parasite-infected ***:*** infection seems to elicit an early programmed cell death in the ovarian follicle cells as well as induces the modulation on the activities of different peptidase classes in either ovaries or fat bodies and the overexpression of the vitellogenin in the ovary of ***.

关键词： Trypanosoma rangeli Rhodnius prolixus Ovarian follicle Vitellogenin Aspartic protease Cysteine protease

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Extracellular vesicles-coupled miRNAs from oviduct and uterus modulate signaling pathways related to lipid metabolism and bovine early embryo development

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1528-1549页

作者： rosane Mazzarella Karina Canon‑Beltran Yulia N.Cajas Meriem Hamdi Encina M.Gonzalez Juliano C.da Silveira Claudia l.V.leal D.Rizos Department of Animal Reproduction INIA-CSICMadridSpain Department of Biochemistry and Molecular Biology Veterinary FacultyComplutense University of Madrid(UCM)MadridSpain Department Agrarian Production Technical University of MadridUPMMadridSpain Departamento de Ciencias Biologicas Universidad Tecnica Particular de LojaUTPLLojaEcuador Department of Anatomy and Embryology FV-UCMMadridSpain Department of Veterinary Medicine FZEA-USPPirassunungaBrazil

Background Extracellular vesicles(EVs) present in oviductal(OF) and uterine fluid(UF) have been shown to enhance bovine embryo quality during in vitro culture by reducing lipid contents and modulating lipid metabolism-related genes(lMGs), while also influencing cell proliferation, suggesting their involvement on the regulation of different biological pathways. The regulation of signaling pathways related to cell differentiation, proliferation, and metabo-lism is crucial for early embryo development and can determine the success or failure of the pregnancy. Bioactive molecules within EVs in maternal reproductive fluids, such as micro RNAs(miRNAs), may contribute to this regulatory process as they modulate gene expression through post-transcriptional *** This study evaluated miRNA cargo in OF-EVs from the early luteal phase and UF-EVs from the mid-luteal phase, coinciding with embryo transit within oviduct and uterus in vivo, and its possible influence on lMGs and sign-aling pathways crucial for early embryo development. A total of 333 miRNAs were detected, with 11 exclusive to OF, 59 to UF, and 263 were common between both groups. From the 20 differentially expressed miRNAs, 19 up-regulated in UF-EVs(bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 and bta-miR-708) and one(bta-miR-148b) in OF-EVs. These miRNAs were predicted to modulate several pathways such as Wnt, Hippo, MAPK, and lipid metabolism and degradation. Differ-ences in miRNAs found in OF-EVs from the early luteal phase and UF-EVs from mid-luteal phase may reflect differ-ent environments to meet the changing needs of the embryo. Additionally, miRNAs may be involved, particularly in the uterus, in the regulation of embryo lipid metabolism, immune system, and *** Our study su

关键词： Embryo-maternal interaction Epigenetic Exosomes Mammalian Preimplantation Reproductive fluids

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Rainfall partitioning in young clonal plantations Eucalyptus species in a subtropical environment,and implications for water and forest management

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International Soil and Water Conservation Research 2021年第3期9卷 474-484页

作者： Decio Oscar Cardoso Ferreto JoséMiguel Reichert rosane Barbosa lopes Cavalcante Raghavan Srinivasan Soils Department Universidade Federal de Santa Maria(UFSM).Avenida Romima 1000Santa MariaRSBrazil Instituto Tecnoldgico Vale.Rua Boaventura da Silva 955UmarizalBelemPABrazil Department of Ecology and Conservation Biology Texas A&M University(TAMU)534 John Kimbrough Blvd.Room 305College StationTXUSA

Different canopy characteristics of industrial eucalyptus may lead to differences in water evaporation and availability to *** study aims to understand rainfall partitioning in a young clonal plantation(age of 2-4.5 years)of three eucalyptus species by relating tree parameters:diameter at breast height,total height,and leaf area *** measured rainfall,throughfall,stemflow and litter interception,along with the tree *** eucalyptus trees had rainfall interception varying between 22 mm(for 178 mm of rainfall)and 42 mm(for 87 mm of te rainfall),throughfall between 106 mm(for 186 mm of rainfall)and 44 mm(for 74 mm of rainfall),and stemflow between 0.5 mm(for 92 mm of rainfall)and 1.4 mm(for 24 mm of rainfall).For the three species,rainfall interception varied between 12 and 48%,throughfall between 57 and 90%,and stemflow between 0.3 and 5.4%.The coefficient of determination between interception and rainfall was 0.76,indicating interception depends on other variables,possibly including antecedent rainfall,rainfall intensity,and *** decreased with a reduction in leaf area index caused by eucalyptus *** *** had 0.75 mm of throughfall per 1 mm of rainfall,whereas in *** and E saligna,these ratios were 0.71 and 0.68,*** in *** and *** had a higher positive relationship with the diameter at breast height of the trees,whereas in *** the highest relationship was with the *** results contribute to establishing management strategies,such as choosing suitable eucalyptus species to local climate,and to improve the synchronization of crop-demand versus soil-water-supply while maintaining streamflow to fulfill ecological and production needs.

关键词： Water balance Blue-green water Forest hydrology Rainfall interception Rainfall throughfall

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Hyperonic neutron stars: reconciliation between nuclear properties and NICER and lIGO/VIRGO results

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Communications in Theoretical Physics 2022年第1期74卷 57-63页

作者： luiz l.lopes Centro Federal de Educagao Tecnologica de Minas Gerais Campus VIII CEP 37.022-560Varginha-MG-Brasil

Using an extended version of quantum hadrodynamics,I propose a new microscopic equation of state(EoS)that is able to correctly reproduce the main properties of symmetric nuclear matter at the saturation density,as well as produce massive neutron stars and satisfactory results for the radius and the tidal parameter.I show that this EoS can reproduce at least a 2.00 solar mass neutron star,even when hyperons are *** constraints about the radius of a 2.00 M_(⊙) and the minimum mass that enables a direct Urea effect are also checked.

关键词： neutron star nuclear matter tidal deformability DU process hyperons

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COMPARISON　OF　SEDIMENT　DISCHARGE　PREDICTIONS　FOR　SMAll　WATERSREDS　IN　THE　SOUTRWESTERN　UNITED　STATES

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International Journal of Sediment Research 1996年第1期 22-33页

作者： Yakov WIGDOR Vicente l.lopes and Peter F.FFOllIOTT

ＣＯＭＰＡＲＩＳＯＮ　ＯＦ　ＳＥＤＩＭＥＮＴ　ＤＩＳＣＨＡＲＧＥ　ＰＲＥＤＩＣＴＩＯＮＳ　ＦＯＲ　ＳＭＡＬＬ　ＷＡＴＥＲＳＲＥＤＳ　ＩＮ　ＴＨＥ　ＳＯＵＴＲＷＥＳＴＥＲＮ　ＵＮＩＴＥＤ　ＳＴＡＴＥＳＹａｋｏｖＷＩＧＤ... 详细信息

ＣＯＭＰＡＲＩＳＯＮ　ＯＦ　ＳＥＤＩＭＥＮＴ　ＤＩＳＣＨＡＲＧＥ　ＰＲＥＤＩＣＴＩＯＮＳ　ＦＯＲ　ＳＭＡＬＬ　ＷＡＴＥＲＳＲＥＤＳ　ＩＮ　ＴＨＥ　ＳＯＵＴＲＷＥＳＴＥＲＮ　ＵＮＩＴＥＤ　ＳＴＡＴＥＳＹａｋｏｖＷＩＧＤＯＲ；ＶｉｃｅｎｔｅＬ．ＬＯＰＥＳ...

关键词： Sediment discharge Total-load formulas Upland watersheds Southwestern United States

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Multidimensional Projections for Visual Analysis of Social Networks

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Journal of Computer Science & Technology 2012年第4期27卷 791-810页

作者： Rafael Messias Martins Gabriel Faria Andery Henry Heberle Fernando Vieira Paulovich Alneu de Andrade lopes Helio Pedrini rosane Minghim Institute of Mathematics and Computer Science University of Sao PauloSao Carlos 13566-590Brazil CPM Braxis Capgemini Sao Paulo 04543-900Brazil Institute of Computing University of CampinasCampinas-SP 13083-852Brazil

Visual analysis of social networks is usually based on graph drawing algorithms and tools. However, social networks are a special kind of graph in the sense that interpretation of displayed relationships is heavily dependent on context. Context, in its turn, is given by attributes associated with graph elements, such as individual nodes, edges, and groups of edges, as well as by the nature of the connections between individuals. In most systems, attributes of individuals and communities are not taken into consideration during graph layout, except to derive weights for force-based placement strategies. This paper proposes a set of novel tools for displaying and exploring social networks based on attribute and connectivity mappings. These properties are employed to layout nodes on the plane via multidimensional projection techniques. For the attribute mapping, we show that node proximity in the layout corresponds to similarity in atgribute, leading to easiness in locating similar groups of nodes. The projection based on connectivity yields an initial placement that forgoes force-based or graph analysis algorithm, reaching a meaningful layout in one pass. When a force algorithm is then applied to this initial mapping, the final layout presents better properties than conventional force-based approaches. Numerical evaluations show a number of advantages of pre-mapping points via projections. User evaluation demonstrates that these tools promote ease of manipulation as well as fast identification of concepts and associations which cannot be easily expressed by conventional graph visualization alone. In order to allow better space usage for complex networks, a graph mapping on the surface of a sphere is also implemented.

关键词： social network visual exploration multidimensional visualization

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Event-based hydrology and sedimentation in paired watersheds under commercial eucalyptus and grasslands in the Brazilian Pampa biome

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International Soil and Water Conservation Research 2021年第2期9卷 180-194页

作者： Ederson Diniz Ebling Jose Miguel Reichert Jhon Jairo Zuluaga Pelaez Miriam Fernanda Rodrigues Mirian lago Valente rosane Barbosa lopes Cavalcante Paolo Reggiani Raghavan Srinivasan Soils Department Universidade Federal de Santa MariaAvenida Roraima1000 Bairro CamobiSanta MariaRS97105-900Brazil Corporacion Colombiana de Investigacion Agropecuaria(AGROSAVIA) Centro de Investigacion NataimaEspinalColombia CMPC Celulose Riograndense Rua Sao Geraldo1680GuaibaRS92500-000Brazil Department of Civil Engineering University of SiegenPaul Bonatz Strasse 9-11Siegen D57068Germany Department of Ecology and Conservation Biology Texas A&M University(TAMU)534 John Kimbrough Blvd.Room 305College StationTX77843-2120USA Current address:Instituto Tecnologico Vale.Rua Boaventura da Silva 955UmarizalBelemPABrazil.

Ecological and hydrological impacts are expected when watersheds are under commercial *** this study we evaluated event-based hydrology and sedimentation in paired watersheds under commercial eucalyptus and grasslands ***,suspended sediment concentration,and sediment production were measured and simulated with the limburg Soil Erosion Model(lISEM)in paired hydrographic watersheds located in southern Brazil;one occupied with 7-years old Eucalyptus saligna plantation(forest watershed-FW;0.83 km^(2))and another Pampa biome with native and exotic grasses used for beef cattle production(grassland watershed-GW;1.10 km^(2)).The effect of rainfall on hydrological and sedimentological variables was more evident in GW compared with *** shape of the hydrograph was steepest in GW,with rapid,marked increase and decrease in flow during *** parameters changed for model calibration in FW and GW were soil saturated hydraulic conductivity,initial soil moisture,and Manning roughness coefficient,whereby the statistical performance was classified as good(NSE>0.75).Fourteen events different from those used in the calibration process were validated for FW and GW,with NSE index classified as“good”and“very good”for *** study shows limitations of lISEM in calibrating and validating the sediment yield for individual events due to the spatial variability of factors controlling soil *** validated modeling results of this study may be applied in simulating soil and crop scenarios in watersheds cultivated with commercial forest and grassland for grazing.

关键词： Southern grasslands Paired watersheds Runoff Erosion lISEM

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Housing flexibility problem： Review of recent limitations and solutions

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Frontiers of Architectural Research 2018年第1期7卷 80-91页

作者： Sabine Ritter De Paris Carlos Nuno l. lopes Centro de Inovaco em Arquitectura e Modos de Habitar (CIAMH) University of Porto Porto 4150-564 Portugal

The economic, technological, and cultural transformations of our present society influence various scales of our daily lives. In housing, these transformations include changes in the family nucleus and the activities performed in living spaces. Housing flexibility seeks to meet the muitipte needs of users by changing their living spaces and functions. This study aims to explore housing flexibility through a review of relevant literature, discussion of valuable findings, and presentation of a contemporary panorama of the theme. Notions of flexibility are integrated to reinterpret the contemporary sense of dwelling. However, the lack of a well-defined concept of flexibility hinders the integration of methods and theories on the subject.

关键词： Housing Fiexibility Building

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Extracellular vesicles-coupled miRNAs from oviduct and uterus modulate signaling pathways related to lipid metabolism and bovine early embryo development.

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畜牧与生物技术杂志(英文版) 2024年第4期 1528页

作者： rosane Mazzarella KarinaCañón-Beltrán Yulia N.Cajas Meriem Hamdi Encina M.González Juliano C.da Silveira Claudia l.V.leal D.Rizos

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Evaluation of calcined textile sludge as a stabilizing material for highway soil

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Journal of Traffic and Transportation Engineering(English Edition) 2020年第5期7卷 688-699页

作者： Alice Gonzaga de Oliveira Amanda Diniz Barros leda Christiane de Figueiredo lopes lucena Adriano Elisio de Figueiredo lopes lucena Jonny Dantas Patricio Department of Civil Engineering Federal University of Campina GrandeCampina Grande 58429140Brazil

The textile industry is a pioneer in the industrialization process in Brazil and is relevant to the Brazilian economy. However, it also generates as operational residue a solid waste,commonly called textile sludge. Pavement construction, which mobilizes large volumes of soil, constitutes an alternative to the reuse of residue, especially when stabilization is involved. The use of stabilized soils with the addition of waste can provide technical,economic and environmentally friendly advantages. This research aimed to study the use of chemical stabilization technique with sludge and three additives(lime, cement and asphalt emulsion) applied in base and subbase of pavements. The scope of this study was divided into four phases, i) characterization tests, ii) physical stabilization, iii) chemical stabilization and determination of heavy metals in the sludge, and iv) addition of 10% of textile sludge and chemical stabilizers(lime, cement, and asphalt emulsion) in amounts of 3%, 5% and 7% as additives in a soil, based on the results obtained in the second phase. Test results indicated that the stabilization of mixtures of soil with textile sludge has the potential to be used in pavement layers(base and subbase). The addition of cement promotes the best chemical stabilization for the sludge. The use of textile sludge stabilization presents greater environmental benefits, given the malfunctions and problems that the incorrect waste disposal can cause to the environment.

关键词： Road engineering Soil stabilization Additives Textile sludge Pavement materials Chemical stabilization

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