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In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients

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World Journal of Transplantation 2018年第1期8卷 23-37页

作者： Francisco Boix Santiago Llorente Jorge Eguía Gema Gonzalez-Martinez Rafael Alfaro Jose A Galián Jose A Campillo María rosa Moya-quiles Alfredo Minguela Jose A Pons Manuel Muro Department of Immunology Clinical University Hospital Virgen de la Arrixaca-IMIBClinical University Hospital‘Virgen Arrixaca’ Department of Nephrology Clinical University Hospital‘Virgen de la Arrixaca-IMIB’Clinical University Hospital‘Virgen Arrixaca’ Digestive Medicine Service Clinical University Hospital‘Virgen de la Arrixaca-IMIB’Clinical University Hospital‘Virgen Arrixaca’

AIM To validate intracellular cytokine production functional assay as means of cell-mediated immunity monitoring of post-transplant patients with opportunistic infection(OI).METHODS Intracellular cytokine-producing CD4^+ and CD8^+ T-cell monitoring was carried out in 30 liver transplant(LTr) and 31 kidney transplant(KTr) recipients from 2010 to 2012. Patients were assessed in our Department of Immunology at the Clinical University ‘Hospital Virgen de la Arrixaca-IMIB' in Murcia, Spain for one year following transplantation. FACS Canto Ⅱ flow cytometer was employed to quantify the intracellular production of IL-17, IFNγ and IL-10 cytokines on stimulated CD4^+CD69^+ and CD8^+CD69^+ T cells and BD FACS DIVA v.6 software was used to analysed the data. Statistical analysis was carried out using SPSS *** LTr with OI had significantly lower % of CD8^+CD69^+IFNγ^+T cells at 60(7.95 ± 0.77 vs 26.25 ± 2.09, P < 0.001), 90(7.47 ± 1.05 vs 30.34 ± 3.52, P < 0.001) and 180(15.31 ± 3.24 vs 24.59 ± 3.28, P = 0.01) d posttransplantation. Higher % of CD4^+CD69^+IL-10^+ as well as CD4^+CD69^+IL-17^+ T cells were yet reported at 30(14.06 ± 1.65 vs 6.09 ± 0.53, P = 0.0007 and 4.23 ± 0.56 vs 0.81 ± 0.14, P = 0.005; respectively), 60(11.46 ± 1.42 vs 4.54 ± 0.91, P = 0.001 and 4.21 ± 0.59 vs 1.43 ± 0.42, P = 0.03; respectively) and 90 d(16.85 ± 1.60 vs 4.07 ± 0.63, P < 0.001 and 3.97 ± 0.43 vs 0.96 ± 0.17, P = 0.001). Yet, KTr with OI had significantly lower percentage of CD4^+CD69^+IFNγ^+ at 30(11.80 ± 1.59 vs 20.64 ± 3.26, P = 0.035), 60(11.19 ± 1.35 vs 15.85 ± 1.58, P = 0.02), 90(11.37 ± 1.42 vs 22.99 ± 4.12, P = 0.028) and 180(13.63 ± 2.21 vs 21.93 ± 3.88, P = 0.008) d post-transplantation as opposed to CD4^+CD69^+IL-10^+ and CD8^+CD69^+IL-10^+ T cells which percentages were higher at 30(25.21 ± 2.74 vs 8.54 ± 1.64, P < 0.001 and 22.37 ± 1.35 vs 17.18 ± 3.54, P = 0.032; respectively), 90(16.85 ± 1.60 vs 4.07 ± 0.63, P < 0.001 and 23.06 ± 2.89 vs 10.19 ± 1.98, P = 0.002) and 18

关键词： Intracellular cytokine Liver transplantation Kidney transplantation Opportunistic infection

On the Use of Monotonicity-Preserving Interpolatory Techniques in Multilevel Schemes for Balance Laws

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Communications on Applied Mathematics and Computation 2024年第2期6卷 1319-1341页

作者： Antonio Baeza rosa Donat Anna Martinez-Gavara Department de Matematiques Universitat de ValenciaDr.Moliner5046100 BurjassotValenciaSpain Department d'Estadistica i Investigacio Operativa Universitat de ValenciaDr.Moliner5046100BurjassotValenciaSpain

Cost-effective multilevel techniques for homogeneous hyperbolic conservation laws are very successful in reducing the computational cost associated to high resolution shock capturing numerical *** they do not involve any special data structure,and do not induce savings in memory requirements,they are easily implemented on existing codes and are recommended for 1D and 2D simulations when intensive testing is *** multilevel technique can also be applied to balance laws,but in this case,numerical errors may be induced by the *** present a series of numerical tests that point out that the use of monotonicity-preserving interpolatory techniques eliminates the numerical errors observed when using the usual 4-point centered Lagrange interpolation,and leads to a more robust multilevel code for balance laws,while maintaining the efficiency rates observed forhyperbolic conservation laws.

关键词： Hyperbolic balance laws Well-balanced schemes Multilevel schemes Harten's multiresolution

Rheological and stability analysis of cement pastes incorporating silica-based wastes

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Particuology 2024年第6期89卷 144-152页

作者： Julia da rosa Martins Janaíde Cavalcante Rocha Dachamir Hotza Luciano Senff Graduate Program in Materials Science and Engineering(PPGMAT) Federal University of Santa Catarina(UFSC)Florianópolis88040-900SCBrazil Graduate Program in Civil Engineering(PPGEC) Federal University of Santa Catarina(UFSC)Florianópolis88040-900SCBrazil Graduate Program in Chemical Engineering(POSENQ) Federal University of Santa Catarina(UFSC)Florianópolis88040-900SCBrazil Department of Mobility Engineering Federal University of Santa Catarina(UFSC)Joinville89219-600SCBrazil

This study investigates the influence of waste characteristics,especially zeta potential,on the properties of cement pastes and *** focus is to evaluate the impact of the zeta potential of cement particles and waste materials on the sedimentation speed,rheology,and hardening time of stabilized cement *** Cement II F 40,retarder additive,silica,and fly ash were used in the *** pastes were prepared,and during the stabilization period,their rheological properties and pH were *** zeta potential and sedimentation speed of the cement and waste particles were measured at the pH that the pastes presented during the entire stabilization *** the stabilization period,the pastes were subjected to the hardening time *** zeta potential analyses revealed diverse values for the different powder types,with the cement particles exhibiting a zeta potential of−3.0 mV,the silica particles exhibiting−10.5 mV,and the fly ash particles exhibiting−20.3 *** influence of the high zeta potential modulus was observed on the sedimentation speed,with the solution containing fly ash exhibiting a speed of 40.01μm/s,whereas the solution containing only cement exhibited a speed of 99.38μm/*** the pastes,the results indicate that the presence of fly ash particles with a significantly negative zeta potential led to a 16%reduction in hardening time compared to particles with a lower modulus of zeta *** tests showed that the inclusion of fly ash particles prevented the formation of *** the zeta potential influenced agglomerate formation and hardening time,it was found to have no effect on yield stress or viscosity.

关键词： Wastes Zeta potential Setting time Rheometry

A High-Order Localized Artificial Diffusivity Scheme for Discontinuity Capturing on 1D Drift-Flux Models for Gas-Liquid Flows

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Advances in Applied Mathematics and Mechanics 2024年第1期16卷 47-74页

作者： Adyllyson H.Nascimento Eugenio S.rosa Department of Energy School of Mechanical EngineeringUniversity of CampinasCampinasBrazil

A computational code is developed for the numerical solution of onedimensional transient gas-liquid flows using drift-flux models,in isothermal and also with phase change *** these two-phase models,classical upwind schemes such as Roe-and Godunov-type schemes are generally difficult to derive and expensive to use,since there are no treatable analytic expressions for the Jacobian matrix,eigenvalues and eigenvectors of the system of *** the other hand,the highorder compact finite difference scheme becomes an attractive alternative on these occasions,as it does not make use of any wave propagation information from the system of *** present paper extends the localized artificial diffusivity method for high-order compact finite difference schemes to solve two-phase flows with *** numerical method has simple formulation,straightforward implementation,low computational cost and,most importantly,*** numerical methodology proposed is validated by solving several numerical examples given in the *** simulations are sixth-order accurate and it is shown that the proposed numerical method provides accurate approximations of shock waves and contact *** is an essential property for simulations of realistic mass transport problems relevant to operations in the petroleum industry。

关键词： Compressible two-phase flows drift-flux model localized artificial diffusivity highorder numerical methods

Lower Continental Crusts Involved in the Alpine Orogeny:New Insight from the Santa Lucia Nappe Metasediments(Corsica,France)

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Acta Geologica Sinica(English Edition) 2024年第4期98卷 904-920页

作者： Chiara FRASSI Maria DI rosa Luca PANDOLFI Michele MARRONI Dipartimento di Scienze della Terra Universitàdi PisaVia Santa Maria 5356126 PisaItaly Istituto di Geoscienze e Georisorse IGG-CNRVia Moruzzi50124 PisaItaly

In the Alpine Corsica(France),the Santa Lucia Nappe represents a peculiar unit preserving the unique relicts of Paleozoic lower to medium continental *** consists of composite basement affected by Permian granulite facies metamorphic conditions unconformably covered by a Late Cretaceous clastic sequence(Tomboni Conglomerate and Tralonca Flysch)affected by polyphase deformation and *** this work,we present a new reconstruction of the deformation events registered by the Tralonca Flysch during the Alpine *** D1 phase was testified by rare isoclinal *** D2 phase produced a continuous foliation and a map-scale sheath-fold with a top-to-W sense of *** D3 phase produced E-verging non-cylindrical folds and S3 crenulation cleavage that is not associated to metamorphic *** present the first temperature-pressure-deformation(P-T-d)path for the Tralonca Flysch,demonstrating that the Santa Lucia Nappe underwent accretion and then first stage exhumation in the Alpine wedge during the D1 phase with pressure and temperature peaks both occurred under blueschist metamorphic *** D2 phase occurred at lower pressure-temperature conditions during a second stage *** pressure-temperaturedeformation path is comparable with those of the Lower Units(i.e.,the subducted continental units of Alpine Corsica)suggesting a common geodynamic history.

关键词： blueschist-facies metamorphism P-T-d path Santa Lucia Nappe Corsica Alpine orogeny

The Ti-TAN plasmid toolbox for TurboID-based proximity labeling assays in Nicotiana benthamiana

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Journal of Integrative Plant Biology 2024年第2期66卷 166-168页

作者： Huang Tan Yu Zhou Erik Dinius rosa Lozano-Durán Department of Plant Biochemistry Center for Plant MolecularBiology(ZMBP)Eberhard Karls UniversityTubingenD‐72076Germany

Protein-protein interactions and the formation of protein complexes play a central role in the regulation of virtually all aspects of *** identification and characterization of interactions between proteins is the bas... 详细信息

关键词： proximity complexes enable

Two viral proteins translated from one open reading frame target different layers of plant defense

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Plant Communications 2024年第4期5卷 177-193页

作者： Yuzhen Mei Tao Hu Yaqin Wang rosa Lozano-Duran Xiuling Yang Xueping Zhou State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhouZhejiang 310058China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Biochemistry Center for Plant Molecular Biology(ZMBP)Eberhard Karls University72076 TubingenGermany

Multilayered defense responses are activated upon pathogen *** utilize a number of strategies to maximize the coding capacity of their small genomes and produce viral proteins for infection,including suppression of host ***,we reveal translation leakage as one of these strategies:two viral effec-tors encoded by tomato golden mosaic virus,chloroplast-localized C4(cC4)and membrane-associated C4(mC4),are translated from two in-frame start codons and function cooperatively to suppress ***4 localizes in chloroplasts,to which it recruits NbPUB4 to induce ubiquitination of the outer membrane;as a result,this organelle is degraded,and chloroplast-mediated defenses are ***,chloroplast-localized cC4 induces the production of singlet oxygen(1O2),which in turn promotes translo-cation of the 1O2 sensor NbMBS1 from the cytosol to the nucleus,where it activates expression of the CERK1 ***,an antiviral effect exerted by CERK1 is countered by mC4,localized at the plasma ***4,like cC4,recruits NbPUB4 and promotes the ubiquitination and subsequent degradation of CERK1,suppressing membrane-based,receptor-like kinase-dependent ***,this translation leakage strategy seems to be conserved in multiple viral species and is related to host *** suggests that stacking of different cellular antiviral responses could be an effective way to abrogate viral infection and engineer sustainable resistance to major crop viral diseases in thefield.

关键词： geminivirus C4 PUB4 CERK1 subcellular localization antiviral defense

Anomalous negative magnetoresistance in quantum dot Josephson junctions with Kondo correlations

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Science China(Physics,Mechanics & Astronomy) 2024年第8期67卷 3-13页

作者： Mingtang Deng Chunlin Yu Guangyao Huang rosa López Philippe Caroff Sepideh Ghalamestani Gloria Platero Hongqi Xu Institute for Quantum Information&State Key Laboratory of High Performance Computing College of Computer Science and TechnologyNational University of Defense TechnologyChangsha 410073China NanoLund and Division of Solid State Physics Lund UniversityLund S-22100Sweden China Greatwall Quantum Laboratory Changsha 410006China Institut de Física Interdisciplinària i de Sistemes Complexos IFISC Palma de Mallorca E-07122Spain Instituto de Ciencia de Materiales de Madrid Madrid 28049Spain Beijing Key Laboratory of Quantum Devices Key Laboratory for the Physics and Chemistry of Nanodevicesand Department of ElectronicsPeking UniversityBeijing 100871China Beijing Academy of Quantum Information Sciences Beijing 100193China Hefei National Laboratory Hefei 230088China

The interplay between superconductivity and the Kondo effect has stimulated significant interest in condensed matter *** compete when their critical temperatures are close and can give rise to a quantum phase transition that can mimic Majorana zero ***,we have fabricated and measured Al-InSb nanowire quantum dot-Al *** the Kondo regime,a supercurrent-induced zero-bias conductance peak *** zero-bias peak shows an anomalous negative magnetoresistance(NMR)at weak magnetic *** attribute this anomalous NMR to quasiparticle trapping at vortices in the superconductor leads as a weak magnetic field is *** trapping effect lowers the quasiparticle-caused dissipation and thus enhances the Josephson *** work connects the vortex physics and the supercurrent tunneling in Kondo regimes and can help further understand the physics of Josephson quantum dot system.

关键词： quantum dot Kondo correlation InSb nanowire quasiparticle trapping

Interactions between maternal parity and feed additives drive the composition of pig gut microbiomes in the post‑weaning period

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1241-1257页

作者： Kayla Law Eduardo rosa Medina Garcia Chad Hastad Deborah Murray Pedro E.Urriola Andres Gomez Department of Animal Science University of Minnesota1364 Eckles AvenueSaint PaulMN 55108USA New Fashion Pork 164 Industrial ParkwayJacksonMN 56143USA

Background Nursery pigs undergo stressors in the post-weaning period that result in production and welfare *** challenges disproportionately impact the offspring of primiparous sows compared to those of mul-tiparous *** is known regarding potential interactions between parity and feed additives in the post-weaning period and their effects on nursery pig ***,the objective of this study was to investigate the effects of maternal parity on sow and offspring microbiomes and the influence of sow parity on pig fecal microbi-ome and performance in response to a prebiotic *** weaning,piglets were allotted into three treat-ment groups:a standard nursery diet including pharmacological doses of Zn and Cu(Con),a group fed a commercial prebiotic only(Preb)based on an Aspergillus oryzae fermentation extract,and a group fed the same prebiotic plus Zn and Cu(Preb+ZnCu).Results Although there were no differences in vaginal microbiome composition between primiparous and mul-tiparous sows,fecal microbiome composition was different(R^(2)=0.02,P=0.03).The fecal microbiomes of primiparous offspring displayed significantly higher bacterial diversity compared to multiparous offspring at d 0 and d 21 post-weaning(P<0.01),with differences in community composition observed at d 21(R^(2)=0.03,P=0.04).When analyzing the effects of maternal parity within each treatment,only the Preb diet triggered significant microbiome distinc-tions between primiparous and multiparous offspring(d 21:R^(2)=0.13,P=0.01;d 42:R^(2)=0.19,P=0.001).Composi-tional differences in pig fecal microbiomes between treatments were observed only at d 21(R^(2)=0.12,P=0.001).Pigs in the Con group gained significantly more weight throughout the nursery period when compared to those in the Preb+ZnCu *** Nursery pig gut microbiome composition was influenced by supplementation with an Aspergillus oryzae fermentation extract,with varying effects on performance

关键词： Aspergillus prebiotic Copper Feed additives Maternal Nursery pig microbiome Parity Swine microbiome Trace minerals Zinc