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Uric formaldehyde levels are negatively correlated with cognitive abilities in healthy older adults

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Neuroscience Bulletin 2014年第2期30卷 172-184页

作者： Jing Yu Tao Su Ting Zhou Yingge he Jing Lu Juan Li rongqiao he Center on Ageing Psychology Key Laboratory of Mental Health Institute of Psychology Chinese Academy of Sciences Faculty of Psychology Southwest University State Key Laboratory of Brain and Cognitive Science Institute of Biophysics Chinese Academy of Sciences The University of Chinese Academy of Sciences

Recent studies have shown that the abnormal accumulation of endogenous formaldehyde could be a critical factor in age-related cognitive decline. The aim of this study was to estimate the correlation between uric formaldehyde and general cognitive abilities in a community-based elderly population, and to measure the extent and direction in which the correlation varied with demographic characteristics. Using a double-blind design, formaldehyde in human urine was analyzed by high-performance liquid chromatography （n = 604）, and general cognitive abilities were measured using the Montreal Cognitive Assessment （MoCA）. Demographic characteristics, in terms of age, gender, residential region, and education were taken into consideration. We found that uric formaldehyde levels were inversely correlated with the MoCA score, and the concentration varied with demographic features： higher odds of a high formaldehyde level occurred among the less educated and those living in old urban or rural areas. In cytological experiments, the level of cellular formaldehyde released into the medium increased as SH-SY5Y and BV2 cells were incubated for three days. Formaldehyde in excess impairedthe processes of N2a cells and neurites of primary cultured rat hippocampal cells. However, removal of formaldehyde markedly rescued and regenerated the processes of N2a cells. These results demonstrated a negative correlation between the endogenous formaldehyde and general cognitive abilities. High formaldehyde levels could be a risk factor for cognitive impairment in older adults, and could be developed as a non-invasive marker for detection and monitoring of age-related cognitive impairment.

关键词： uric formaldehyde the MontrealCognitive Assessment education learning livingregion

Transcriptome profile of human neuroblastoma cells in the hypomagnetic field

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Science China(Life Sciences) 2014年第4期57卷 448-461,1-3页

作者： MO WeiChuan LIU Ying BARTLETT Perry F he rongqiao Institute of Biophysics Chinese Academy of Sciences Queensland Brain Institute The University of QueenslandBrisbane

Research has shown that the hypomagnetic field(HMF)can affect embryo development,cell proliferation,learning and memory,and in vitro tubulin *** the present study,we aimed to elucidate the molecular mechanism by which the HMF exerts its effect,by comparing the transcriptome profiles of human neuroblastoma cells exposed to either the HMF or the geomagnetic field.A total of 2464 differentially expressed genes(DEGs)were identified,216 of which were up-regulated and2248 of which were down-regulated after exposure to the *** DEGs were found to be significantly clustered into several key processes,namely macromolecule localization,protein transport,RNA processing,and brain *** DEGs were verified by real-time quantitative PCR,and the expression levels of nine of these DEGs were measured every 6 *** notably,MAPK1 and CRY2,showed significant up-and down-regulation,respectively,during the first 6 h of HMF exposure,which suggests involvement of the MAPK pathway and cryptochrome in the early bio-HMF *** results provide insights into the molecular mechanisms underlying the observed biological effects of the HMF.

关键词： hypomagnetic field geomagnetic field transcriptome profile massively parallel sequencing MAPK1 CRY2

A lumbrokinase isozyme targets hepatitis B e-antigen

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Science China(Life Sciences) 2018年第12期61卷 1596-1598页

作者： Yuan Zhou Xiumei Wang Shichao Fan rongqiao he Institute of Biophysics University of Chinese Academy of Sciences

Dear Editor, hepatitis B Virus(HBV)infection remains a major health problem worldwide despite the availability of a highly ef- fective preventive *** genome codes several distinct proteins,among which the non-particulate e-antigen(HBeAg)is one of the important markers in HBV infection. Sera containing HBeAg are highly infectious but those with anti-HBeAg have little infectivity.

关键词： hepatitis B Virus(HBV) little infectivity

D-ribose increases triglyceride via upregulation of DGAT in the liver

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Science China(Life Sciences) 2019年第6期62卷 858-861页

作者： Yao Chen Lexiang Yu Yan Wei Yang Long Yong Xu Tao he rongqiao he School of Basic Medical Sciences of Southwest Medical University Luzhou 646000 China State Key Laboratory of Brain and Cognitive Science Institute of Biophysics University of Chinese Academy of Sciences Beijing 100101 China Affiliated Hospital of Southwest Medical University Luzhou 646000 China CAS Key Laboratory of Mental health Institute of Psychology Chinese Academy of Sciences Beijing 100101 China

Dear Editor, Fatty liver disease or hepatic steatosis, recognized as the hepatic component of metabolic syndrome, can progress to cirrhosis and hepatocellular carcinoma (Youngwanichsetha, 2018).

关键词： Dear Editor hepatic steatosis progress to cirrhosis

Autofluorescence of MDA-modified proteins as an in vitro and in vivo probe in oxidative stress analysis

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Protein & Cell 2014年第6期5卷 484-487页

作者： Qiang, Min Xu, Yajie Lu, Yang he, Yingge Han, Chanshuai Liu, Ying he, rongqiao Chinese Acad Sci Inst Biophys State Key Lab Brain & Cognit Sci Beijing 100101 Peoples R China Chinese Acad Sci Inst Psychol Key Lab Mental Hlth Beijing 100101 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China

Malondialdehyde （MDA） is regarded as a biomarker for oxidative stress （Del Rio et al., 2005; Stancliffe et al., 2011 ）, and is generated in the oxidative degradation process of polyunsaturated lipids （Stancliffe et al., 2011）. This compound, a reactive electrophile species, contains two aldehyde groups and exhibits cytotoxic effects. As a product of lipid peroxidation, MDA was found to accumulate during many pathophysiological processes （Miller et al., 2011 ）,

关键词： Bovine Serum Albumin Coomassie Brilliant Blue Staining Bovine Serum Albumin Sample Oxidative Stress Analysis Oxidative Degradation Process

Visualizing the microtubule-associated protein tau in the nucleus

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Science China(Life Sciences) 2014年第4期57卷 422-431页

作者： LU Jing LI Ting he rongqiao BARTLETT Perry F GTZ Jürgen Clem Jones Centre for Ageing Dementia Research Queensland Brain Institute The University of QueenslandBrisbane Institute of Biophysics Chinese Academy of Sciences

Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular *** tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse *** human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s ***,in wild-type mice,despite its abundance,tau is difficult to visualize even in the *** is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from *** establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse *** depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma *** a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated *** either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear *** subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic *** our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form.

关键词： Alzheimer's disease fractionation microtubule-associated protein neuroblastoma nucleus phosphorylation tau

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早期综合干预有助延缓阿尔茨海默症的发生

科学通报 2016年第32期61卷 3420-3427页

作者：赫荣乔中国科学院生物物理研究所脑与认知科学国家重点实验室北京100101

阿尔茨海默症(Alzheimer’s disease,AD)的典型病变包括认知异常、脑内amyloid?(A?)沉积形成的老年斑(senile plaque)、异常磷酸化Tau蛋白形成的神经纤维缠结(neurofibrillary tangles,NFTs)、胶质细胞激活以及脑萎缩.在近100多年的研... 详细信息

阿尔茨海默症(Alzheimer’s disease,AD)的典型病变包括认知异常、脑内amyloid?(A?)沉积形成的老年斑(senile plaque)、异常磷酸化Tau蛋白形成的神经纤维缠结(neurofibrillary tangles,NFTs)、胶质细胞激活以及脑萎缩.在近100多年的研究过程中,国内外同行把研究重点集中在AD的临床阶段,追求单一靶点的药物.然而,这些努力尚未在临床治疗上实现重大突破.当前,AD临床前阶段(preclinical phases)的早期病理变化和干预措施研究受到了相当的重视.为了延缓老年痴呆的发生,在强调多靶点药物研发的同时,其他干预方法,包括改善生活习惯、调节饮食、参与社会活动、进行适当的体育锻炼等,也在AD的防治中得以研究与应用.

关键词：阿尔茨海默症认知功能损害老年斑神经纤维缠结多靶点干预

D-ribose,an overlooked player in type 2 diabetes mellitus?

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Science China(Life Sciences) 2014年第3期57卷 361-361页

作者： SU Tao he rongqiao State Key Laboratory of Brain and Cognitive Science Institute of BiophysicsChinese Academy of Sciences University of Chinese Academy of Sciences

Type 2 diabetes mellitus is a metabolic disorder that is characterized by high blood glucose due to either insulin resistance or insulin deficiency[1].A direct correlation between D-glucose and diabetic complications has long been established,and is the focus of most research in this *** contrast,D-Ribose has been overlooked so far as a potential risk player in the development of diabetes.

关键词： 2型糖尿病 D-核糖胰岛素抵抗球员糖尿病并发症 D-葡萄糖代谢紊乱潜在风险

The herbal compound geniposide rescues formaldehyde-induced apoptosis in N2a neuroblastoma cells

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Science China(Life Sciences) 2014年第4期57卷 412-421,1-3页

作者： CheN JinYan SUN MengRu WANG XingHua LU Jing WEI Yan TAN Yan LIU Ying GTZ Jürgen he rongqiao HUA Qian Beijing University of Chinese Medicine Clem Jones Centre for Ageing Dementia Research Queensland Brain Institute The University of QueenslandBrisbane Institute of Biophysics Chinese Academy of Sciences

The herbal medicine Tong Luo Jiu Nao （TLJN） contains geniposide （GP） and ginsenoside Rgl at a molar ratio of i0：1. Rgl is the major component of another herbal medicine, panax notoginseng saponin （PNS）. TLJN has been shown to strengthen brain function in humans, and in animals it improves learning and memory. We have previously shown that TLJN reduces amyloi- dogenic processing in Alzheimer＇s disease （AD） mouse models. Together this suggests TLJN may be a potential treatment for patients with dementia. Because chronic damage of the central nervous system by formaldehyde （FA） has been presented as a risk factor for age-associated cognitive dysfunction, in the present study we investigated the protective effect of both TLJN and GP in neuron-like cells exposed to FA. FA-exposed murine N2a neuroblastoma cells were incubated with TLJN, its main in- gredient GP, as well as PNS, to measure cell viability and morphology, the rate of apoptosis and expression of genes encoding Akt, FOXO3, Bcl2 and p53. The CCK-8 assay, cytoskeletal staining and flow cytometry were used to test cell viability, mor- phology and apoptosis, respectively. Fluorescent quantitative real-time PCR （qRT-PCR） was used to monitor changes in gene expression, and HPLC to determine the rate of FA clearance. Treatment of N2a cells with 0.09 mmol L-1 FA for 24 h signifi- cantly reduced cell viability, changed cell morphology and promoted apoptosis. Both TLJN and GP conferred neuroprotection to FA-treated N2a cells, whereas PNS, which had to be used at lower concentrations because of its toxicity, did not. Our data demonstrate that TLJN can rescue neuronal damage caused by FA and that its main ingredient, GP, has a major role in this ef- ficacy. This presents purified GP as a drug or lead compound for the treatment of AD.

关键词： Alzheimer's disease apoptosis formaldehyde geniposide neuroprotection panax notoginseng saponin Tong Luo Jiu Nao