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Arachnoid cell involvement in the mechanism of coagulation-initiated inflammation in the subarachnoid space after subarachnoid hemorrhage

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第7期11卷 516-523页

作者： Zhao-liang XIN Xiao-kang WU Jian-rong xu Xi LI Department of Neurosurgery Zhejiang Medical College Hangzhou 310053 China Department of Neurosurgery Shanghai Tenth People's Hospital Shanghai 200072 China Department of Neurosurgery Yiwu Central Hospital Yiwu 322000 China

Objective： To assess if arachnoid cells have the capability to present antigen and activate T-lymphocytes after stimulation by bloody cerebrospinal fluid （CSF）, and to illuminate the mechanism of coagulation-initiated inflammation in the subarachnoid space after subarachnoid hemorrhage （SAH）. Methods： Arachnoid cells were cultured, characterized, and examined by immunofluorescence for the basal expression of human leukocyte antigen-DR （HLA-DR）, Expression of HLA-DR, after co-culturing arachnoid cells in vitro with bloody CSF, was investigated by immunofluorescence and flow cytometry （FCM）. The variation of arachnoid cells＇ ultrastructure was observed by transmission electron microscope （TEM）. Arachnoid cells were co-cultured with peripheral blood mononuclear cells （PBMCs）. The content of soluble interleukin-2 receptor （slL-2r） in culture medium was detected by enzyme-linked immunosorbent assay （ELISA）. Results：（1） Arachnoid cells were successfully cultured for many passages. The immunofluorescent staining was positive for HLA-DR in over 95% of the human arachnoid cells. The punctate HI_A-DR was distributed in cytoplasm and not in the karyon. （2） After co-culturing arachnoid cells in vitro with bloody CSF, numerous particles with strong fluorescence appeared in the cytoplasm on Day 6. On Day 8, the quantity of particles and fluorescent intensity were maximal. FCM showed that the percentage of HLA-DR expressing cells was （2.5＋_0.4）% at the first 5 d, increasing to （60.8＋_3.6）% on Day 7. （3） After co-culturing arachnoid cells in vitro with bloody CSF, many lysosome and secondary lysosome particles were present in the cytoplasm. Hyperplasia of rough endoplasmic re- ticulum and enlarged cysts were observed, with numerous phagocytizing vesicles also observed at the edge of the arachnoid cells. （4） Arachnoid cells stimulated by bloody CSF were co-cultured in vitro with PBMCs. The content of slL-2r in the culture medium, having been maintained at around 1.30 ng/ml during t

关键词： Arachnoid cells Cell culture Human leukocyte antigen-DR (HLA-DR) Soluble intedeukin-2 receptor (slL-2r)

Two new nimbolinin-and trichilin-class limonoids isolated from the fruits of Melia azedarach

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Chinese Journal of Natural Medicines 2019年第3期17卷 227-230页

作者： QIU Lu HENG Li xu rong LUO Jun LI Yi Testing & Analysis Center Nanjing Normal University Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines China Pharmaceutical University

Two new furan fragment isomerized limonoids, meliazedalides A and B(compounds 1 and 2), were isolated from the fruits of Melia azedarach Linn.. Their chemical structures were elucidated on the basis of HR-ESI-MS and 1D and 2D NMR data, which belonged to nimbolinin-and trichilin-class, respectively. Compound 2 exhibited weak inhibitory effect on NO production in lipopolysaccharide(LPS)-activated RAW 264.7 macrophages with IC_(50) being 37.41 μmol·L^(–1).

关键词： Melia azedarach Meliaceae Limonoid Anti-inflammatory

Domain interactions of C-terminal Src Kinase determined through NMR spectroscopy with segmental isotope labeling

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Protein & Cell 2017年第1期8卷 67-71页

作者： Dongsheng Liu Ya Yuan rong xu David Cowburn Human Institute ShanghaiTech University 99 Haike RoadPudong Shanghai 201210 China Department of Biochemistry Albert Einstein College of Medicine1300 Morris Park Avenue Bronx NY 10461 USA

Dear Editor, Proteins with large molecular weight exhibit increased number of NMR signals, and the resulting spectral overlap typically imposes limitations.

关键词： Domain determined labeling

Effects of photoperiod and temperature on diapause induction in Conogethes punctiferalis （Lepidoptera： Pyralidae）

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Insect Science 2014年第5期21卷 556-563页

作者： Li-rong xu Xinzhi Ni Zhen-Ying Wang Kang-Lai He The State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences China USDA-ARS Crop Genetics and Breeding Research Unit University of Georgia-Tifton Campus Tifton GeorgiaUSA

The yellow peach moth, Conogethes punctiferalis （Guenee）, a multivoltine species that overwinters as diapausing larvae, is one of the most serious insect pests on maize in China. Effect of photoperiod and temperature on larval diapause was examined under empirical laboratory conditions. Short-day treatments caused larval diapause at 25℃, and the critical photoperiod was between 12 and 13 h （or 12 h 51 min） light per day. No sensitive instar was identified for diapause induction under alternated short- （L ： D 11 ： 13 h） and long-day （L ： D 14 ： 10 h） treatments at different larval stages. However, accumulative treatment of three instars and 10 d under short-day treatment was required for the induction of 50% larval diapause. All larvae entered diapause at 20℃, whereas less than 3% did so at 30℃, irrespective of the long- or short-day treatment. Furthermore, under the short-day treatment, more than 90% of larvae went into diapause with temperatures ≤ 25℃, but less than 17% did so at 28℃. In contrast, under the long-day treatment, less than 19% of larvae went into diapause with temperatures ≥23 ℃. The forward shift （5℃） of critical temperature under the long-day regime demonstrated the compensatory effect of temperature and photoperiod on diapause induction. In conclusion, C. punctiferalis had a temperature-dependent type Ⅰ photoperiodic diapause response; there was no sensitive instar for diapause determination, but the photoperiodic accumulation time countermeasures both of the short-day cycles and the number ofinstars exposed, and the photoperiodic diapause response, was a temperature-compensated phenomenon.

关键词： Conogethes punctiferalis diapause induction photoperiod sensitive larval instar temperature dependence yellow peach moth

EXPERIMENTAL AND THEORETICAL STUDY OF CAVITATION-INDUCED MECHANICAL EFFECT ON A SOLID BOUNDARY

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Journal of Hydrodynamics 2005年第6期17卷 724-729页

作者： xu rong-qing CHEN Xia SHEN Zhong-hua LU Jian NI Xiao-wu School of Electronics and information Jiangsu University of Science and Technology Zhenjiang 212003 China Department of Applied Physics Nanjing University of Science and Technology Nanjing 210094 China

The mechanical effect induced by the cavitati- on bubble collapse in the neighborhood of a solid boundary was investigated by focusing a Q-switched laser pulse on a metal target in water. By means of a fiber-coupling optical beam deflection technique, the displacement generated by liquid jet impact at the final stage of the bubble collapse was detected at the epicenter of the rear metal surface. Furthermore, by combining a widely used laser ablation model with the detection principles of this detector, the transient impact force exerting on the target material could be easily estimated. Besides, according to experimental results and the modified Ray leigh theory, the maximum bubble radius and the liquid-jet pressure were also obtained, which are in good agreement with previous results,

关键词： liquid jet impact optical beam deflection laser ablation model cavitation

Tetraphenylethylene dicycle helicates: circularly polarized luminescence and chiral recognition based on emission wavelength change

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Science China Chemistry 2024年第11期67卷 3817-3824页

作者： Zhi-rong xu Miao-Li Gao Ming Hu Wei Yu Yan-Song Zheng Key Laboratory of Material Chemistry for Energy Conversion and Storage Ministry of EducationSchool of Chemistry and Chemical EngineeringHuazhong University of Science and TechnologyWuhan 430074China

Chiral tetraphenylethylene(TPE) dicycle tetraaldehyde and TPE dicycle tetraacids bearing optically pure groups on the cycles were designed and synthesized. Due to the propeller-like conformation immobilization of TPE unit, this new class of TPE dicycle was resolved into M-and P-enantiomers, which could emit strong circular polarized luminescence(CPL). Interestingly,these TPE helicates displayed exceptional ability of molecule recognition. While the TPE dicycle tetraaldehyde could detect the microscale water in anhydrous tetrahydrofuran, the chiral TPE dicycle tetraacid could display different fluorescent color after interacting with two enantiomers of a wide variety of chiral amines including monoamines, diamines, and aminols, which could be applied to chiral recognition of these chiral amines. Furthermore, the emission wavelength of TPE dicycle tetraacid was found to change linearly with the enantiomer purity of chiral amine from enantiomer excess(ee) percent-100% to +100%, for the first time showing the potential for quantitative chiral analysis of chiral amines based on emission wavelength change. The emission wavelength was affected less by environmental factors than fluorescence intensity, which would enable the chiral analysis based on wavelength change with higher accuracy and repeatability.

关键词： TPE dicycle helicate CPL chiral recognition enantiomer excess analysis emission wavelength change

On-site variety discrimination of tomato plant using visible-near infrared reflectance spectroscopy

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第2期10卷 126-132页

作者： Hui-rong xu Peng YU Xia-ping FU Yi-bin YING College of Biosystems Engineering and Food Science Zhejiang University Hangzhou 310029 China

The use of visible-near infrared (NIR) spectroscopy was explored as a tool to discriminate two new tomato plant varieties in China (Zheza205 and Zheza207). In this study, 82 top-canopy leaves of Zheza205 and 86 top-canopy leaves of Zheza207 were measured in visible-NIR reflectance mode. Discriminant models were developed using principal component analysis (PCA), discriminant analysis (DA), and discriminant partial least squares (DPLS) regression methods. After outliers detection, the samples were randomly split into two sets, one used as a calibration set (n=82) and the remaining samples as a validation set (n=82). When predicting the variety of the samples in validation set, the classification correctness of the DPLS model after optimizing spectral pretreatment was up to 93%. The DPLS model with raw spectra after multiplicative scatter cor- rection and Savitzky-Golay filter smoothing pretreatments had the best satisfactory calibration and prediction abilities (correlation coefficient of calibration (Rc)=0.920, root mean square errors of calibration=0.196, and root mean square errors of predic- tion=0.216). The results show that visible-NIR spectroscopy might be a suitable alternative tool to discriminate tomato plant varieties on-site.

关键词： Visible-NIR spectroscopy Tomato plant variety Discrimination Principal component analysis (PCA) Discriminant analysis (DA) Discriminant partial least squares (DPLS)

Improving Water Use Efficiency of Wheat Crop Varieties in the North China Plain: Review and Analysis

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Journal of Integrative Agriculture 2013年第7期12卷 1243-1250页

作者： MEI xu-rong ZHONG Xiu-li Vadez Vincent LIU Xiao-ying Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural Sciences/State Engineering Laboratory of Efficient Water Use and Disaster Mitigation for Crops/Key Laboratory for Dryland Agriculture Ministry of Agriculture International Crop Research Institute for the Semi-Arid Tropics (ICRISAT)

The North China Plain （NCP）, one of the most important agricultural regions in China, is facing a major water-resource crisis evoked by excessive exploitation of groundwater. To reduce water use while maintaining high crop production level, improving variety water use efficiency （WUE） is an urgent need, especially because other water-saving measures such as water delivery, irrigation, and agricultural practices have already achieved most possible progresses. Evaluation of variety WUE can be performed accurately at the individual plant level （WUEp）. Reviewing the studies on physiological factors affecting WUE p performed up to date, stomatal conductance was considered to be an important trait associating closely with WUE p . The trait showed a large degree of varietal variability under well-watered conditions. Crop varieties differ highly in sensitivity of stomata to soil and air drying, with some varieties strongly reducing their stomatal conductance in contrast with those lightly regulating their stomata. As a result, difference among varieties in WUE p was enlarged under water deficit conditions in contrast with those under well-watered conditions. The relationship between stomatal conductance and yield depends on water availability of whole growing period in local areas. Usually, large stomatal conductance results in a high yield under good irrigation system, whereas a low stomatal conductance can lead to yield benefit under limited stored soil moisture conditions. In the NCP, winter wheat is the largest consumer of irrigation water, improvement strategies for high WUE aiming at wheat crops are in urgent need. We suggest, for the well-irrigated areas with excessive exploitation of groundwater, the wheat breeding program need to combine medium stomatal conductance （0.35 mmol H2O m-2 s-1 or so）, high carboxylation efficiency, and high harvest index. Areas with partial/full access to irrigation, or infrequent drought, should target wheat varieties with high stomatal

关键词： water use efficiency yield stomatal conductance water deficit

Usefulness of CT angiography in diagnosing acute gastrointestinal bleeding:A meta-analysis

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World Journal of Gastroenterology 2010年第31期16卷 3957-3963页

作者： Lian-Ming Wu Jian-rong xu Yan Yin Xin-Hua Qu Department of Radiology Ren Ji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127China

AIM: To analyze the accuracy of computed tomography (CT) angiography in the diagnosis of acute gastrointestinal (GI) bleeding. METHODS: The MEDLINE, EMBASE, Cancerlit, Cochrane Library database, Sciencedirect, Springerlink and Scopus, from January 1995 to December 2009, were searched for studies evaluating the accuracy of CT angiography in diagnosing acute GI bleeding. Studies were included if the ycompared CT angiography to a reference standard of upper GI endoscopy, colonoscopy, angiography or surgery in the diagnosis of acute GI bleeding. Meta-analysis methods were used to pool sensitivity and specificity and to construct summary receiver-operating characteristic. RESULTS: A total of 9 studies with 198 patients were included in this meta-analysis. Data were used to form 2 × 2 tables. CT angiography showed pooled sensi-tivity of 89% (95% CI: 82%-94%) and specificity of 85% (95% CI: 74%-92%), without showing significant heterogeneity (χ2 = 12.5, P = 0.13) and (χ2 = 22.95, P = 0.003), respectively. Summary receiver operating characteristic analysis showed an area under the curve of 0.9297. CONCLUSION: CT angiography is an accurate, costeffective tool in the diagnosis of acute GI bleeding and can show the precise location of bleeding, thereby directing further management.

关键词： Angiography Gastrointestinal hemorrhage Meta-analysis Sensitivity and specifi city Tomography