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禽白血病病毒p27蛋白单克隆抗体制备及抗原表位的鉴定

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动物医学进展 2025年第1期46卷 23-28页

作者：陈桂娥容芳苟鑫孙荣航李作生陈瑞爱岭南现代农业科学与技术广东省实验室肇庆分中心广东肇庆526238 华南农业大学生命科学学院广东广州510642 华南农业大学兽医学院广东广州510642 华农(肇庆)生物产业技术研究院有限公司广东肇庆526238 肇庆大华农生物药品有限公司广东肇庆526238

为制备禽白血病病毒(ALV)p27蛋白特异性单克隆抗体,利用原核表达系统表达并纯化ALV p27蛋白,将其作为免疫原免疫注射Balb/c小鼠,用杂交瘤细胞融合和ELISA等筛选出阳性单克隆抗体细胞,对制备的单克隆抗体的抗体亚型、效价及特异性进行鉴... 详细信息

为制备禽白血病病毒(ALV)p27蛋白特异性单克隆抗体,利用原核表达系统表达并纯化ALV p27蛋白,将其作为免疫原免疫注射Balb/c小鼠,用杂交瘤细胞融合和ELISA等筛选出阳性单克隆抗体细胞,对制备的单克隆抗体的抗体亚型、效价及特异性进行鉴定,将目的蛋白截短成4段,初步鉴定单克隆抗体识别的抗原表位区域。结果表明,共筛选出4株单克隆细胞,其抗体亚类皆为IgG1型,其中3A1抗体效价为1∶64000,3B2为1∶256000,5F1为1∶128000,5G2为1∶8000;4株单克隆抗体识别的抗原表位在1-60 aa之间。研究结果为进一步建立ALV相关免疫学检测方法提供了重要的材料。

关键词：禽白血病病毒 p27蛋白单克隆抗体抗原表位

A Biomimetic Study of Discontinuous-Constraint Metamorphic Mechanism for Gecko-Like Robot

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Journal of Bionic Engineering 2007年第2期4卷 91-95页

作者： Zhen-dong Dai Jiu-rong sun Institute of Bio-inspired Structure and Surface Engineering The Academy of Frontier Science Nanjing University of Aeronautics and Astronautics Nanjing 210016 P. R. China College of Life Sciences Peking University Beijing 100871 P. R. China

Locomotion ability, efficiency and reliability are key targets for a good robot. The linkage mechanism for robot locomotion is a discontinuous-constraint metamorphic mechanism. Here we set up equations to present the discontinuous-constraint, point out that driving and controlling are the key points to improve the performance and efficiency of the linkage mechanism. Inspired by controlling strategy of the motor nervous system in peripheral vertebrae to the locomotion, we draw off motor control and drive strategy.

关键词： biomimetic robotics gecko-like robot metamorphic mechanism

Improved astrometry of space debris with image restoration

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Research in Astronomy and Astrophysics 2014年第8期14卷 992-1000页

作者： rong-Yu sun Chang-Yin Zhao Key Laboratory of Space Object and Debris Observation Chinese Academy of Sciences Purple Mountain Observatory Chinese Academy of Sciences

In order to implement an observing strategy, image degradation that occurs during optical observation of space debris is ineluctable and has distinct characteris- tics. Image restoration is presented as a way to remove the influence of degradation in CCD images of space debris, based on assumed PSF models with the same F-WHM as images of the object. In the process of image restoration, the maximum entropy method is adopted. The results of reduction using observed raw CCD images indi- cate that the precision in estimating positions of objects is improved and the effects of degradation are reduced. Improving the astrometry of space debris using image restoration is effective and feasible.

关键词： techniques image processing -- space vehicles -- astrometry -- methods data analysis

A new source extraction algorithm for optical space debris observation

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Research in Astronomy and Astrophysics 2013年第5期13卷 604-614页

作者： rong-Yu sun Chang-Yin Zhao Purple Mountain Observatory Chinese Academy of Sciences Key Laboratory of Space Object and Debris Observation Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences

Specific challenges arise in the task of real-time automatic data reduction of optical space debris observations. Here we present an automatic technique that optimally detects and measures the sources from images of optical space debris ob- servations. We show that highly reliable and accurate results can be obtained on most images produced by our specific sensors, and due to optimizations, the whole pipeline works fast and efficiently. Tests demonstrate that the technique performs better than SExtractor from the point of view of fast and accurate detection, therefore it is well suited for data reduction of optical space debris observations.

关键词： techniques: image processing -- space vehicles -- astrometry

Alcohol and Metabolic-associated Fatty Liver Disease

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Journal of Clinical and Translational Hepatology 2021年第5期9卷 719-730页

作者： Fu-rong sun Bing-Yuan Wang Department of Elderly Gastroenterology The First Hospital of China Medical UniversityShenyangLiaoningChina

The diagnosis of metabolic-associated fatty liver disease is based on the detection of liver steatosis together with the presence of metabolic *** to this new definition,the diagnosis of metabolic-associated fatty liver disease is independent of the amount of alcohol ***,alcohol and its metabolites have various effects on metabolicassociated abnormalities during the process of alcohol *** have shown improved metabolic function in light to moderate alcohol *** are several studies focusing on the role of light to moderate alcohol intake on metabolic ***,the results from studies are diverse,and the conclusions are often *** review systematically discusses the effects of alcohol consumption,focusing on light to moderate alcohol consumption,obesity,lipid and glucose metabolism,and blood pressure.

关键词： Alcohol Metabolic-associated fatty liver disease Obesity Insulin resistance Hypertension

Effect of the combination of high-frequency repetitive magnetic stimulation and neurotropin on injured sciatic nerve regeneration in rats

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Neural Regeneration Research 2020年第1期15卷 145-151页

作者： Jie Chen Xian-Ju Zhou rong-Bin sun Department of Orthopedics the Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical UniversityChangzhouJiangsu ProvinceChina Laboratory of Neurological Diseases Department of Neurologythe Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical UniversityChangzhouJiangsu ProvinceChina Department of Neurology Integrated Hospital of Traditional Chinese MedicineSouthern Medical UniversityGuangzhouGuangdong ProvinceChina

Repetitive magnetic stimulation is effective for treating posttraumatic neuropathies following spinal or axonal *** is a potential treatment for nerve injuries like demyelinating *** study sought to observe the effects of high-frequency repetitive magnetic stimulation,neurotropin and their combined use in the treatment of peripheral nerve injury in 32 adult male Sprague-Dawley *** create a sciatic nerve injury model,a 10 mm-nerve segment of the left sciatic nerve was cut and rotated through 180°and each end restored continuously with interrupted *** rats were randomly divided into four *** control group received only a reversed autograft in the left sciatic nerve with no *** the high-frequency repetitive magnetic stimulation group,peripheral high-frequency repetitive magnetic stimulation treatment(20 Hz,20 min/d)was delivered for 10 consecutive days after *** the neurotropin group,neurotropin therapy(0.96 NU/kg per day)was administrated for 10 consecutive days after *** the combined group,the combination of peripheral high-frequency repetitive magnetic stimulation(20 Hz,20 min/d)and neurotropin(0.96 NU/kg per day)was given for 10 consecutive days after the *** Basso-Beattie-Bresnahan locomotor rating scale was used to assess the behavioral recovery of the injured *** sciatic functional index was used to evaluate the recovery of motor *** blue staining was performed to determine the number of myelinated fibers in the distal and proximal *** staining was used to detect the length of axons marked by neurofilament *** results reveal that the Basso-Beattie-Bresnahan locomotor rating scale scores,sciatic functional index,the number of myelinated fibers in distal and proximal grafts were higher and axon lengths were longer in the high-frequency repetitive magnetic stimulation,neurotropin and combined groups compared with the control ***

关键词： axon myelinated nerve fibers nerve regeneration neurological rehabilitation neurotropin peripheral nerve injury repetitive magnetic stimulation sciatic nerve trauma

Emerging low-density polyethylene/paraffin wax/aluminum composite as a form-stable phase change thermal interface material

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International Journal of Minerals,Metallurgy and Materials 2023年第4期30卷 772-781页

作者： Chuanchang Li Weixuan Wang Xiaoliang Zeng Chunxuan Liu rong sun School of Energy and Power Engineering Changsha University of Science and TechnologyChangsha 410114China Shenzhen Institute of Advanced Electronic Materials Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Hunan Xiangtou Light Material Technology Co. Ltd.Changsha 410012China

Thermal interface materials(TIMs) play a vital role in the thermal management of electronic devices and can significantly reduce thermal contact resistance(TCR). The TCR between the solid–liquid contact surface is much smaller than that of the solid–solid contact surface, but conventional solid–liquid phase change materials are likely to cause serious leakage. Therefore, this work has prepared a new formstable phase change thermal interface material. Through the melt blending of paraffin wax(PW) and low-density polyethylene(LDPE), the stability is improved and it has an excellent coating effect on PW. The addition of aluminum(Al) powder improves the low thermal conductivity of PW/LDPE, and the addition of 15wt% Al powder improves the thermal conductivity of the internal structure of the matrix by 67%. In addition, the influence of the addition of Al powder on the internal structure, thermal properties, and phase change behavior of the PW/LDPE matrix was systematically studied. The results confirmed that the addition of Al powder improved the thermal conductivity of the material without a significant impact on other properties, and the thermal conductivity increased with the increase of Al addition. Therefore, morphologically stable PW/LDPE/Al is an important development direction for TIMs.

关键词： paraffin wax low-density polyethylene phase change materials thermal interface materials form stability

Temporal and Spatial Distribution of DIN and DIP Concentrations and Source Apportionment Along the Bohai Sea of China During 2015-2022

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Chinese Geographical Science 2024年第6期34卷 1004-1016页

作者： ZHANG Xinjie TIAN Chongguo sun Zeyu YIN Xuehua sun rong WANG Juying Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China State Environmental Protection Key Laboratory of Land and Sea Ecological Governance and Systematic Regulation Shandong Academy for Environmental PlanningJinan 250101China Shandong Key Laboratory of Coastal Environmental Processes Yantai 264003China University of Chinese Academy of Sciences Beijing 100049China National Marine Environmental Monitoring Center Dalian 116023China

The Bohai Sea is one of the most polluted sea areas in *** this study,we used 2184 integrated concentrations of dissolved inorganic nitrogen(DIN)and dissolved inorganic phosphorus(DIP)in the Bohai Sea of China during spring(March,April,and May),summer(June,July,and August),and autumn(October and November)from 2015 to 2022 to explore the trends and sources of nutrients *** 2015 to 2022,DIN showed a downward trend until 2020 and then an upward trend,whereas DIP exhibited a stable trend with a slight *** concentrations of DIN and DIP had similar seasonal pattern which was the highest in autumn(0.292±0.247 mg/L for DIN and 0.013±0.016 mg/L for DIP)but lower in spring(0.267±0.238 mg/L for DIN and 0.006±0.010 mg/L for DIP)and summer(0.263±0.324 mg/L for DIN and 0.008±0.010 mg/L for DIP).Sources of DIN and DIP apportioned by the positive matrix factorization(PMF)model were riverine input,sediment resuspension,sewage discharge,atmospheric deposition,and underground *** 2015-2022,the largest contributor to DIN was sewage discharge(28.7%)and the largest contributor to DIP was sediment resuspension(44.6%).Seasonally,DIN in spring and autumn was dominated by sewage discharge(45.4%and 27.8%,re-spectively).Whereas in summer,it was dominated by riverine input(32.4%)and atmospheric deposition(29.7%).DIP was dominated by sediment resuspension during all three seasons(35.8%-52.5%).In addition,the increase in DIN concentrations in 2021 and 2022 were mainly due to the incremental input of river discharge and atmospheric deposition caused by increased precipitation during sum-mer and autumn.

关键词： dissolved inorganic nitrogen(DIN) dissolved inorganic phosphorus(DIP) source apportionment positive matrix factoriza-tion(PMF)model the Bohai Sea of China

Deep Neural Network Closed-loop with Raw Data for Optical Resident Space Object Detection

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Research in Astronomy and Astrophysics 2024年第11期24卷 95-103页

作者： He Zhao rong-Yu sun Sheng-Xian Yu Purple Mountain Observatory Chinese Academy of SciencesNanjing 210023China School of Astronomy and Space Science University of Science and Technology of ChinaHefei 230026China

Optical survey is an important means for observing resident space objects and space situational *** the application of astronomical techniques and reduction method,wide field of view telescopes have made significant contributions in discovering and identifying resident space ***,with the development of modern optical and electronic technology,the detection limit of instruments and infrastructure has been greatly extended,leading to an extensive number of raw images and many more sources in these *** arise when reducing these data in terms of traditional measurement and *** on the amount of data,it is particularly feasible and reliable to apply machine learning *** an end-to-end deep learning framework is developed,it is trained with a priori information on raw detections and the automatic detection task is performed on the new data *** closed-loop is evaluated based on consecutive CCD images obtained with a dedicated space debris survey *** is demonstrated that our framework can achieve high performance compared with the traditional method,and with data fusion,the efficiency of the system can be improved without changing hardware or deploying new *** technique deserves a wider application in many fields of observational astronomy.

关键词： methods data analysis-techniques image processing-surveys