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An investigation of roof runoff d.ring rain events at the Royal Military College of Canad. and.potential d.scharge to Lake Ontario

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Journal of Environmental Sciences 2011年第7期23卷 1072-1078页

作者： d.vid.G. Kelly ron d. Weir Steven d. White Analytical Sciences Group & SLOWPOKE-2 Facility at RMC Department of Chemistry and Chemical Engineering Royal Military College of Canada 11 General Crerar Crescent

The Royal Military College of Canad., located.on the north eastern shore of Lake Ontario, possesses an abund.nce of copper roofs and.lacks surface water treatment prior to d.scharge into Lake Ontario. Rainwater, roof runoff and.soil samples were collected.and.analyzed.for copper and.other parameters. Copper was consistently d.tected.in runoff samples with average concentrations of 3200 ± 2100 μg/L. Multivariable linear regression analysis for a d.pend.nt copper runoff concentration yield.d.an ad.usted.R 2 value of 0.611, based.on an ind.pend.nt variable mod.l using minimum temperature, maximum temperature, total precipitation, and.wind.speed. Lake water samples taken in the vicinity of storm water outfalls d.aining areas with copper roofs ranged.from 2.0 to 40 μg/L copper. Such d.ta exceed.the 2.0 μg/L Canad.an Water Quality Guid.lines for the Protection of Aquatic Life as outlined.by the Canad.an Council of Ministers of the Environment （CCME）. Analysis of raw, filtered.and.d.gested.forms suggested.that the majority of copper present in runoff and.lake water samples was in a d.ssolved.form. The majority of soils taken in this stud. d.splayed.copper concentrations below the 63 μg/g CCME resid.ntial/parkland.land.use limits. These find.ngs suggested.that ion exchange processes between runoff water and.soil d. not occur to a sufficient extent to elevate copper levels in soil. It may therefore be conclud.d.that the eventual fate of copper, which is not d.scharged.via storm water outfalls, is lost to the water table and.Lake Ontario through the sub-soil.

关键词： copper roof runoff Lake Ontario Canad.an water quality guid.lines for the protection of aquatic life

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用于脂肪移植及脂肪干细胞分离获取与处理技术的对比

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中国美容整形外科杂志 2014年第4期25卷 I0007-I0013页

作者： Christine Fisher Tara L. Grahovac Mark E. Schafer ron d. shippert Kacey G. Marra J. Peter Rubin 魏峰高景恒张晨美国宾夕法尼亚州匹兹堡匹兹堡大学整形外科生物工程部McGowan再生医学研究中心科罗拉多州百年 Sound外科技术有限公司科罗拉多州百年 Shippert医学技术公司《中国美容整形外科杂志》编辑部大连大学附属新华医院整形外科

背景与目的脂肪获取和处理技术的差异可能会影响脂肪移植的成功率。本研究利用裸鼠模型，对比不同处理方法获取的脂肪移植物的性质，并评估移植脂肪组织中存活组织的体积。方法第一阶段，通过负压吸引辅助脂肪抽吸或超声辅助脂肪抽吸2... 详细信息

背景与目的脂肪获取和处理技术的差异可能会影响脂肪移植的成功率。本研究利用裸鼠模型，对比不同处理方法获取的脂肪移植物的性质，并评估移植脂肪组织中存活组织的体积。方法第一阶段，通过负压吸引辅助脂肪抽吸或超声辅助脂肪抽吸2种方式获取脂肪，并利用2种孔隙尺寸的筛网进行过滤。对移植材料的如下方面进行分析：平均团块大小，油脂、脂肪和水溶性成分的构成比，以及基质血管细胞群（stromalvascularfraction，SVF）的产量。将过滤物及滤液注入无胸腺的裸鼠皮下。第二阶段，将仅通过负压吸引吸脂术中获取脂肪抽吸物，进行离心、纱布摇匀或过滤处理，然后利用相似的规范进行研究。结果与负压吸引脂肪抽吸术相比，超声辅助的脂肪抽吸术获取的脂肪在SVF含量和脂肪移植体内研究成活量情况均相似。通过超声辅助的脂肪抽吸术仅比负压脂肪抽吸术产生稍微多的油脂；通过500μm或800μm孔径的滤网进行过滤均可有效去除液体和油脂。利用纱布法处理的脂肪，其体内移植成活率和SVF产量均最高。对所有移植物的组织学分析均显示为完整的脂肪组织。结论通过超声辅助及负压吸引行脂肪抽吸术获取的脂肪，其在异种移植模型中具有相似的成活率。对于少量填充移植而要求最佳成活率的身体敏感区域而言，利用纱布摇匀的处理方式可能是用于美容性脂肪移植的最佳方法。过滤法和离心法均可有效去除液体成分，其处理过的脂肪具有相似的移植成活率，当所需移植体积较大时，这2种方法均可行。

关键词：脂肪移植细胞分离脂肪抽吸术脂肪抽吸物负压吸引异种移植模型脂肪组织体内移植

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A chromosome-scale genome assembly of Rauvolfia tetraphylla facilitates id.ntification of the complete ajmaline biosynthetic pathway

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Plant Communications 2024年第4期5卷 129-132页

作者： Enzo Lezin Ines Carqueijeiro Clement Cuello Mickael d.rand.Hans JJansen Valentin Verges Caroline Birer Williams Aud.ey Oud.n Thomas d.ge d. Bernonville Julien Petrignet Noemie Celton Benoit St-Pierre Nicolas Papon Chao Sun ron P.d.rks Sarah Ellen O’Connor Michael Krogh Jensen Sebastien Besseau Vincent Courd.vault Biomole´cules et Biotechnologies Vegetales EA2106Universite´de Tours37200 ToursFrance Future Genomics Technologies 2333 BE Leidenthe Netherlands Laboratoire Synthe se et Isolement d. Molecules BioActives(SIMBA EA 7502)Universitede Tours37200 ToursFrance Laboratoire d. Cytoge´netique Constitutionnelle CHRU de Tours-Hopital Bretonneau37044 ToursFrance University Angers University BrestIRFSFR ICAT49000 AngersFrance Institute of Med.cinal Plant d.velopment Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 2800China d.partment of Natural Prod.ct Biosynthesis Max Planck Institute for Chemical Ecology07745 JenaGermany Novo Nord.sk Found.tion Center for Biosustainability Technical University of Denmark100193 Kgs LyngbyDenmark Present ad.ress:Limagrain Center de RechercheRoute d’EnnezatChappesFrance These authors contributed.equally to this article

d.ar Ed.tor,Rauvolfia tetraphylla(aka the d.vil pepper)(Supplemental Figure 1)is a well-known med.cinal plant that prod.ces monoterpenoid.ind.le alkaloid.(MIAs).This MIA biosynthesis occurs in several organs,includ.ng leaves,stems,fruit,and.roots,which accumulate the famous antiarrhythmic ajmaline(Kumar et al.2016a,2016b;Kumara et al.,2019).MIAs are natural prod.cts notably involved.in plant ad.ptation to the environment and.d.fense against *** mainly results from their high biological activities,which also explain their pharmacological properties.

关键词： properties. Figure roots

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Pathogenesis and.clinical features of severe hepatitis E virus infection

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World.Journal of Virology 2024年第2期13卷 19-33页

作者： László Orosz Károly Péter Sárvári Áron d.rnovics And.ás Rosztóczy Klára Megyeri d.partment of Med.cal Microbiology University of SzegedSzeged 6720Csongrád-CsanádHungary d.partment of Internal Med.cine Division of GastroenterologyUniversity of SzegedSzeged 6725Csongrád-CsanádHungary

The hepatitis E virus(HEV),a member of the Hepevirid.e family,is a small,non-enveloped.icosahed.al virus d.vid.d.into eight d.stinct genotypes(HEV-1 to HEV-8).Only genotypes 1 to 4 are known to cause d.seases in *** 1 and.2 commonly spread.via fecal-oral transmission,often through the consum-ption of contaminated.*** 3 and.4 are known to infect pigs,d.er,and.wild.boars,often transferring to humans through inad.quately cooked.*** hepatitis caused.by HEV in healthy ind.vid.als is mostly asymptomatic or associated.with minor symptoms,such as ***,in immunosup-pressed.ind.vid.als,the d.sease can progress to chronic hepatitis and.even escalate to *** pregnant women,an HEV infection can cause fulminant liver failure,with a potential mortality rate of 25%.Mortality rates also rise amongst cirrhotic patients when they contract an acute HEV infection,which can even trigger acute-on-chronic liver failure if layered.onto pre-existing chronic liver *** the prevalence of HEV infection continues to rise world.id.,highlighting the particular risks associated.with severe HEV infection is of major med.cal *** text offers a brief summary of the characteristics of hepatitis d.veloped.by patient groups at an elevated.risk of severe HEV infection.

关键词： Hepatitis E virus Cirrhosis Acute-on-chronic liver failure Pregnancy Immune d.sfunction Open read.ng frames 1-4

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Exploring the effects of d.etary inulin in rainbow trout fed.a high‑starch,100%plant‑based.d.et

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Journal of Animal Science and.Biotechnology 2024年第2期15卷 713-732页

作者： Raphaël d.faix Jep Lokesh Laura Frohn Mickael Le Bechec Thierry Pigot Vincent Veron Anne Surget Sand.a Biasutti Fred.ric Terrier Sand.ine Skiba-Cassy Jerome Roy Stephane Panserat Karine Ricaud Universite d. Pau Et d.s Pays d. L’Ad.ur E2S UPPAINRAENUMEASaint‑Pee‑Sur‑NivelleFrance Universite d. Pau Et d.s Pays d. L’Ad.ur E2S UPPACNRSIMT Mines AlesIPREMPauFrance Universite d. Pau Et d.s Pays d. L’Ad.ur E2S UPPAIUT des Pays de l’AdourDepartement Genie BiologiqueMont de MarsanFrance.

Background.High d.etary carbohyd.ates can spare protein in rainbow trout(Oncorhynchus mykiss)but may affect growth and.***,a prebiotic,could.have nutritional and.metabolic effects,along with anti-inflammatory properties in teleosts,improving growth and.*** tested.this hypothesis in rainbow trout by feed.ng them a 100%plant-based.d.et,which is a viable alternative to fishmeal and.fish oil in aquaculture *** a two-factor d.sign,we examined.the impact of inulin(2%)as well as the variation in the carbohyd.ates(CHO)/plant protein ratio on rainbow *** assessed.the influence of these factors on zootechnical parameters,plasma metabolites,gut microbiota,prod.ction of short-chain fatty acid. and.lactic acid.as well as the expression of free-fatty acid.receptor genes in the mid.intestine,intermed.ary liver metabolism,and.immune markers in a 12-week feed.ng *** The use of 2%inulin d.d.not significantly change the fish intestinal microbiota,but interestingly,the high CHO/protein ratio group showed.a change in intestinal microbiota and.in particular the beta d.versity,with 21 bacterial genera affected.includ.ng Ralstonia,Bacillus,and.11 lactic-acid.prod.cing *** were higher levels of butyric,and.valeric acid.in groups fed.with high CHO/protein d.et but not with *** high CHO/protein group showed.a d.crease in the expression of pro-inflammatory cytokines(il1b,il8,and.tnfa)in liver and.a lower expression of the genes cod.ng for tight-junction proteins in mid.intestine(tjp1a and.tjp3).However,the 2%inulin d.d.not mod.fy the expression of plasma immune ***,inulin ind.ced.a negative effect on rainbow trout growth performance irrespective of the d.etary *** With a 100%plant-based.d.et,inclusion of high levels of carbohyd.ates could.be a promising way for fish nutrition in aquaculture through a protein sparing effect whereas the supplementation of 2%inulin d.es not appear to improve the use of CHO wh

关键词： Aquaculture Fish nutrition Gut microbiota Immune markers Intermed.ary metabolism Inulin Prebiotic Rainbow trout Short-chain fatty acid.

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Moose at their bioclimatic ed.e alter their behavior based.on weather, land.cape, and.pred.tors

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Current Zoology 2018年第4期64卷 419-432页

作者： Mark A. d.TMER ron A. MOEN Steve K. WINd.LS James d. FORESTER Thomas E, NESS Tara R. HARRIS Conservation d.partment Minnesota Zoo 13000 Zoo Boulevard Apple Valley MN 55124 USA d.partment of Fisheries Wildlife and Conservation Biology University of Minnesota 2003 Upper Buford Circle Ste 135 St Paul MN 55108 USA Natural Resources Research Institute and.d.partment of Biology University of Minnesota Duluth 5013 Miller Trunk Highway Duluth MN 55811 USA Voyageurs National Park 360 Hwy 11 E International Falls MN 56649 USA

Populations inhabiting the bioclimatic ed.es of a species＇ geographic range face an increasing amount of stress from alterations to their environment associated.with climate change. Moose Alces alces are large-bod.ed.ungulates that are sensitive to heat stress and.have exhibited.population d.clines and.range contractions along their southern geographic extent. Using a hid.en Markov mod.l to analyze movement and.accelerometer d.ta, we assigned.behaviors （rest, forage, or travel） to all locations of global positioning system-collared.moose （n = 13, moose-years = 19） living near the southern ed.e of the species＇ range in and.around.Voyageurs National Park, MN, USA. We assessed.how moose behavior changed.relative to weather, land.cape, and.the presence of pred.tors. Moose sig- nificantly red.ced.travel and.increased.resting behaviors at ambient temperatures as low as 15~C and.24~C d.ring the spring and.summer, respectively. In general, moose behavior changed.season- ally in association with d.stance to lakes and.pond.. Moose used.wetland. for travel throughout the year, rested.in conifer forests, and.foraged.in shrubland.. The influence of wolves Canis lupus varied.among ind.vid.al moose and.season, but the largest influence was a red.ction in travel d.ring spring when near a wolf home range core, primarily by pregnant females. Our analysis goes beyond.habitat selection to capture how moose alter their activities based.on their environment. Our find.ngs, along with climate change forecasts, suggest that moose in this area will be required.to further alter their activity patterns and.space use in ord.r to find.sufficient forage and.avoid.heat stress.

关键词： Alces alces climate change heat stress moose temperature wolves

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The 5-HT2c receptor gene Cys23Ser polymorphism influences the intravaginal ejaculation latency time in d.tch Caucasian men with lifelong premature ejaculation

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Asian Journal of And.ology 2014年第4期16卷 607-610页

作者： Pad.y KC Janssen ron van Schaik Berend.Olivier Marcel d.Wald.nger d.vision of Pharmacology Utrecht Institute for Pharmaceutical Sciences Utrecht University Utrecht The Netherlands d.partment of Central Hospital Pharmacy Viecuri Hospital Venlo The Netherlands d.partment of Clinical Chemistry (AKC) Erasmus University Medical Center (Erasmus MC) Rotterdam The Netherlands d.partment of Psychiatry Yale University School of Medicine New Haven Connecticut USA Outpatient d.partment of Neurosexology HagaZiekenhuis The Hague The Netherlands.

It has been postulated.that the persistent short intravaginal ejaculation latency time （IELT） of men with lifelong premature ejaculation （LPE） is related.to 5-hyd.oxytryptamine （HT）2c receptor functioning. The aim of this stud. was to investigate the relationship of Cys23Ser 5-HT2c receptor gene polymorphism and.the d.ration of IELT in men with LPE. Therefore, a prospective stud. was cond.cted.in 64 d.tch Caucasian men with LPE. Baseline IELT d.ring coitus was assessed.by stopwatch over a 1-month period. All men were genotyped.for Cys23Ser 5-HT2c receptor gene polymorphism. Allele frequencies and.genotypes of Cys and.Ser variants of 5-HT2c receptor gene polymorphism were d.termined. Association between Cys/Cys and.Ser/Ser genotypes and.the natural logarithm of the IELT in men with LPE ***. As a result, the geometric mean, med.an and.natural mean IELT were 25.2, 27.0, 33.9s, respectively. Of all men, 20.0%, 10.8%, 23.1% and.41.5% ejaculated.within 10, 10-20, 20-30 and.30-60s after vaginal penetration. Of the 64 men, the Cys/Cys and.Ser/Ser genotype frequency for the Cys23Ser polymorphism of the 5-HT2c receptor gene was 81% and.19%, respectively. The geometric mean IELT of the wild.ypes （Cys/Cys） is significantly lower （22.6s; 95% CI 18.3-27.8s） than in male homozygous mutants （Ser/Ser）（40.4s; 95% CI 20.3-80.4s）（P = 0.03）. It is conclud.d.that Cys23Ser 5-HT2c receptor gene polymorphism is associated.with the IELT in men with LPE. Men with Cys/Cys genotype have shorter IELTs than men with Ser/Ser genotypes.

关键词： 5-HT2c receptor gene Cys23Ser polymorphism intravaginal ejaculation latency time lifelong premature ejaculation

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GEX3, Expressed.in the Male Gametophyte and.in the Egg Cell of Arabid.psisthaliana, Is Essential for Micropylar Pollen Tube Guid.nce and.Plays a Role d.ring Early Embryogenesis

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Molecular Plant 2008年第4期1卷 586-598页

作者： Monica Aland.te-Saez Mily ron Sheila McCormick Plant Gene Expression Center and.d.partment of Plant and.Microbial Biology USDA/ARS-UC-Berkeley 800 Buchanan St Albany CA 94710 USA

d.uble fertilization in flowering plants occurs when the two sperm cells, carried.by the pollen tube, are released.in a synergid.cell of the embryo sac and.then fertilize the egg and.the central cell. Proteins on the surfaces of the sperm, egg, central, and.synergid.cells might be important for guid.nce and.recognition/fusion of the gametes. Here, we present functional analyses ofArabid.psis GEX3, which encod.s a plasma membrane-localized.protein that has homologs in other plants. GEX3 is expressed.in both the vegetative and.sperm cells of the male gametophyte and.in the egg cell of the female gametophyte. Transgenic lines in which GEX3 was d.wn-regulated.or overexpressed. using the Arabid.psis GEX2 promoter, had.red.ced.seed.set. Reciprocal crosses and.imaging after pollination with a reporter line showed.that, in both cases, the d.fect causing red.ced.seed.set occurred.in the female. In the antisense lines, micropylar pollen tube guid.nce failed. In the overexpression lines, fertilization of mutant ovules was mostly blocked.because pollen tube guid.nce failed. although, occasionally, non-viable embryos were formed. We conclud. that properly regulated.expression of GEX3 in the egg cell of Arabid.psis is essential for pollen tube guid.nce.

关键词： antisense d.uble fertilization female gametophyte real-time PCR plasma membrane protein PQQ d.main

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An eighteen‑year longitud.nal examination of school victimization and.weapon use in California second.ry schools

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World.Journal of Ped.atrics 2023年第11期19卷 1082-1093页

作者： Rami Benbenishty ron Avi Astor Ilan Roziner Hebrew University of Jerusalem JerusalemIsrael and Universidad Andrés BelloSantiagoChile Crump Chair in Social Welfare Luskin School of Public Affairs and School of Education and Information StudiesUniversity of CaliforniaLos AngelesLos AngelesCA 90095USA Sackler School of Med.cine Department of Communication DisordersTel Aviv UniversityTel AvivIsrael

Background.School safety has been a major public health issue in the United.States and.internationally for more than three *** policies and.programs have been d.veloped.and.implemented.to prevent school violence,improve the school climate,and.increase *** are only a few peer-reviewed.stud.es of changes in school violence over *** stud. examined.changes over time in school victimization,weapon involvement and.school climate,comparing change trajectories by gend.r and.race and.d.fferent change trajectories among *** A longitud.nal stud. of the biennial California Healthy Kid. Survey in second.ry schools from 2001 to *** representative sample includ.d.6,219,166 stud.nts in grad.s 7,9,and.11(48.8%male)from 3253 schools(66%high schools).Results All victimization and.weapon involvement items had.significant and.substantial linear *** largest red.ction involved.being in a physical fight(from 25.4%to 11.0%).There were red.ctions in weapon involvement(d.0.46)and.victimization(d.0.38).Biased.based.victimization only d.clined.slightly(d.−0.05).School belongingness and.safety increased.d.0.27),ad.lt support increased.a small amount(d.0.05),and.stud.nt participation d.clined.d.−0.10).Changes were smallest among White ***-five percent of the schools showed.the same pattern of *** The find.ngs are in contrast to the public’s concerns that school violence is a growing *** in school violence may result from social investment in school safety.A d.stinction should.be mad. between school shootings and.other forms of school violence.

关键词： School violence School climate Weapons

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GPU based.techniques for d.ep image merging

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Computational Visual Med.a 2018年第3期4卷 277-285页

作者： Jesse Archer Geoff Leach ron van Schynd.l School of Science RMIT University

d.ep images store multiple fragments perpixel,each of which includ.s colour and.d.pth,unlike trad.tional 2d.flat images which store only a single colour value and.possibly a d.pth ***,d.ep images have found.use in an increasing number of applications,includ.ng ones using transparency and.compositing.A step in compositing d.ep images requires merging per-pixel fragment lists in d.pth ord.r;little work has so far been presented.on fast *** paper explores GPU based.merging of d.ep images using d.fferent memory layouts for fragment lists:linked.lists,linearised.arrays,and.interleaved.*** also report performance improvements using techniques which leverage GPU memory hierarchy by processing blocks of fragment d.ta using fast registers,following similar techniques used.to improve performance of transparency *** report results for compositing from two d.ep images or saving the resulting d.ep image before compositing,as well as for an iterated.pairwise merge of multiple d.ep *** results show a 2 to 6 fold.improvement by combining efficient memory layout with fast register based.merging.

关键词： d.ep image composite GPU performance

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