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Journa. of Forestry Resea.ch 2015年第3期26卷 635-640页

作者： Silvia.Sa.cedo rodrigo a. contreras a.eja.dra.Moenne University of Sa.tia.o of Chile Santiago Chile Fa.ulta. de Ciencia. a.ra.ia. Universidad Te'cnica Estatal de Quevedo 63 Quevedo Ecuador

Oligo-ca.ra.eena.s （OCs） obta.ned from pure ca.ra.eena.s extra.ted from ma.ine red a.ga. stimula.e growth by enha.cing photosynthesis a.d ba.a. meta.olism in toba.co pla.ts a.d Euca.yptus trees. In a.dition, OCs stimula.e seconda.y meta.olism, increa.ing the level of meta.olites involved in defense a.a.nst pa.hogens. In this work, we a.a.yzed the effect of OC ka.pa.on the increa.e in height, in a.tivities of ba.a. meta.olism enzymes in- volved in ca.bon, nitrogen a.d sulphur a.simila.ion, ribu- lose 1,5 biphospha.e ca.boxyla.e/oxygena.e （rubisco）, gluta.a.e dehydrogena.e （GDH） a.d O-a.etylserine thiol- lya.e （Oa.TL）, a.d in the level of growth-promoting hormones, the a.xin indole a.etic a.id （Ia.） a.d the gibberellin Ga., in pine （Pinus ra.ia.a. trees trea.ed with OC ka.pa.a. concentra.ions of 1 a.d 5 mg mL-1 a.d cultiva.ed for 9 months without a.ditiona. trea.ment. Pines trea.ed with OC ka.pa.a. 1 mg mL-1 showed a.simila. increa.e in height but displa.ed a.higher increa.ed in tota. chlorophyll, a.tivities of rubisco, GDH a.d Oa.TL a.d level of Ia. a.d Ga. tha. those trea.ed with OC ka.pa.a. 5 mg mL-1. Thus, OC ka.pa.stimula.es growth a.d ba.a. meta.olism a.d increa.es the level of growth-promoting hormones in pine trees, ma.nly a. 1 mg mL-1.

关键词： a.xin Ba.a. meta.olism C, N a.d Sa.simila.ion Gibberellin Oligo-ca.ra.eena. ka.pa.inus ra.ia.a

Identifica.ion of ISSR ma.kers linked to flowering tra.ts in a.representa.ive sa.ple of Euca.yptus cla.oca.yx

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Journa. of Forestry Resea.ch 2016年第2期27卷 239-245页

作者： rodrigo contreras-Soto Pa.lina.Ba.lesta.Edua.do Ruiz Freddy Mora Depa.tment of a.ronomy State University of Maring Depa.tment of Bota.y University of Concepción Institute of Biologica. Sciences University of Talca

Ea.ly flowering a.d flower a.unda.ce ha.e long been considered desira.le tra.ts in euca.ypt breeding progra.s. In pa.ticula., flowers of Euca.yptus cla.oca.yx provide a.necta. source for the production of honey in a.id ecosystems. To identify inter-simple sequence repea. （ISSR） ma.kers tha. a.e a.socia.ed with ea.ly flowering a.d flower a.unda.ce in the southern a.a.a.a.Desert, we used a.sa.ple of 47 trees, representing five a.stra.ia. prove- na.ces of E. cla.oca.yx. a.unified mixed linea. model （which considered the effect of genetic structure a.d the kinship rela.ionship a.ong trees） revea.ed tha. three loci were significa.tly a.socia.ed with ea.ly flowering, which a.counted for 10-16 % of the phenotypic va.ia.ion, while two loci a.counted for 11-13 % in flowering intensity. Locus ISO1-500 bp wa. a.socia.ed with both flowering tra.ts. This result is consistent with our previous findings indica.ing tha. ma.ker-a.sisted selection on ea.ly flowering should ha.e significa.t a.d positive impa.t on flowering intensity. The a.plica.ion of ma.ker-a.sisted selection to identify trees tha. flower ea.ly a.d intensively ma. increa.e honey production, a.resource tha. genera.es a.ditiona. income for the loca. fa.mers of the southern a.a.a.a.Desert.

关键词： Euca.yptus cla.oca.yx ISSR Floweringtra.ts Ma.ker-a.sisted selection

a.tered expression pa.terns of syndeca.-1 a.d ,2 predicl biochemica. recurrence in prosta.e ca.cer

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a.ia. Journa. of a.drology 2011年第3期13卷 476-480,514页

作者： rodrigo Ledezma.Federico Cifuentes Iva. Ga.legos Jua. Fulla.Enrique Ossa.don Enrique a.Ca.tellon Hector R contreras La.ora.ory of Molecula. a.d Cellula. a.drology Physiology and Biophysics Program. Institute of Biomedical Sciences Faculty of Medicine University of Chile Santiago 70005 Chile pa.hology Service Clinic Hospital University of Chile Santiago 70005 Chile Urology Service Clinic Hospital University of Chile Santiago 70005 Chile

The clinica. fea.ures of prosta.e ca.cer do not provide a. a.cura.e determina.ion of pa.ients undergoing biochemica. rela.se a.d a.e therefore not suita.le a. indica.ors of prognosis for recurrence. New molecula. ma.kers a.e needed for proper pre-trea.ment risk stra.ifica.ion of pa.ients. Our a.m wa. to a.sess the va.ue of a.tered expression of syndeca.-1 a.d -2 a. a.ma.ker for predicting biochemica. rela.se in pa.ients with clinica.ly loca.ized prosta.e ca.cer trea.ed by ra.ica. prosta.ectomy. The expression of syndeca.-1 a.d -2 wa. exa.ined by immunohistochemica. sta.ning in a.series of 60 pa.a.fin-embedded tissue sa.ples from pa.ients with loca.ized prosta.e ca.cer. Ten specimens from pa.ients with benign prosta.ic hyperpla.ia.were used a. non-ma.igna.t controls. Semiqua.tita.ive a.a.ysis wa. performed to eva.ua.e the sta.ning pa.terns. To investiga.e the prognostic va.ue, Ka.la.-Meier surviva. curves were performed a.d compa.ed by a.log-ra.k test. In benign sa.ples, syndeca.-1 wa. expressed in ba.a. a.d secretory epithelia. cells with ba.ola.era. membra.e Ioca.isa.ion, wherea. syndeca.-2 wa. expressed preferentia.ly in ba.a. cells. In prosta.e ca.cer sa.ples, the expression pa.terns of both syndeca.s shifted to gra.ula.-cytopla.mic Ioca.isa.ion. Surviva. a.a.ysis showed a.significa.t difference （P〈0.05） between norma. a.d a.tered expression of syndeca.-1 a.d -2 in free prosta.e-specific a.tigen recurrence surviva. curves. These da.a.suggest tha. the expression of syndeca.-1 a.d -2 ca. be used a. a.prognostic ma.ker for pa.ients with clinica.ly loca.ized orosta.e ca.cer, improving the la.osta.e-specific a.tigen recurrence risk stra.ifica.ion.

关键词： biochemica. recurrence prosta.e ca.cer syndeca.s

Ma.a.ement of nona.coholic fa.ty liver disea.e:a. evidence-ba.ed clinica. pra.tice review

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World Journa. of Ga.troenterology 2014年第34期20卷 12182-12201页

作者： Jua. P a.a. Roberto Ca.dia.rodrigo Za.a.a.Cristián Mu?oz Jua. P a.a.cibia.Ja.me Ponia.hik a.eja.dro Soza.Fra.cisco Fuster Ja.ier Bra.m Edga. Sa.hueza.Jorge contreras M Ca.olina.Cuella. Ma.co a.rese a.noldo Riquelme Depa.ta.ento de Ga.troenterología Escuela de MedicinaPontificia Universidad Católica de Chile8330024 SantiagoChile Sección de Ga.troenterología Hospital del SalvadorEscuela de MedicinaUniversidad de Chile7500922 SantiagoChile Unida. de Ga.troenterología Universidad del DesarrolloClínica Alemana7650568 SantiagoChile Unida. de Tra.pla.te Hepático Hospital del SalvadorUnidad de GastroenterologíaClínica Las Lilas7510524 SantiagoChile Sección de Ga.troenterología Departamento de MedicinaHospital Clínico Universidad de ChileChile8380456 SantiagoChile Sección de Ga.troenterología Hospital Clínico Universidad de ChileEscuela de MedicinaUniversidad de Chile8380456 SantiagoChile Digestive Disea.es Unit Clínica Re?aca2540364 Vi?a del MarChile Ga.troenterology Depa.tment Clínica Las Condes6770128 SantiagoChile Centre for Medica. Educa.ion Escuela de MedicinaPontificia Universidad Católica de Chile8330024 SantiagoChile

a.M: To build a.consensus a.ong Chilea. specia.ists on the a.propria.e ma.a.ement of pa.ients with nona.coholic fa.ty liver disea.e (Na.LD) in clinica. pra.tice.

关键词： Nona.coholic fa.ty liver disea.e Stea.osis Consensus Evidence-ba.ed medicine Trea.ment

Thera.eutic a.terna.ives for the trea.ment of type 1 hepa.orena. syndrome: a.Delphi technique-ba.ed consensus

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World Journa. of Hepa.ology 2016年第25期8卷 1075-1086页

作者： Jua. P a.a. Jua. C Cla.o Jua. P a.a.cibia.Jorge contreras Ferna.do Gomez Cristia. Munoz Leyla.Na.a. Eric Roessler rodrigo Wolff Ma.co a.rese Ca.los Benítez Depa.ta.ento de Ga.troenterología Escuela de Medicina Pontificia Universidad Católica de Chile Depa.ta.ento de Medicina.Interna.a.d Progra.a.de Sa.ud Ba.a.a.en la.Evidencia.Escuela.de Medicina Pontificia Universidad Católica de Chile Sección de Ga.troenterología Departamento de Medicina Hospital Clínico Universidad de Chile and Clínica Santa María Unida. de Ga.troenterología Universidad del Desarrollo Clínica Alemana Servicio de Ga.troentero-logía Unidad de Trasplante Hepático Hospital del Salvador Facultad de Medicina Universidad de Chile and Unidad de Gastroenterología Clínica Alemana de Santiago Clínica Indisa Clínica.La. Condes y Servicio de Ga.troenterología Hospital Fuerza Aérea de Chile Depa.ta.ento de Nefrología Facultad de Medicina Pontificia Universidad Católica de Chile

a.M To propose severa. a.terna.ives trea.ment of type 1 hepa.orena. syndrome(HRS-1) wha. is the most severe expression of circula.ory dysfunction on pa.ients with porta. *** a.group of eleven ga.troenterologists a.d nephrologists performed a.structured a.a.ysis of a.a.la.le *** expert wa. designa.ed to review a.d a.swer a.*** genera.ed dra.t sta.ements for eva.ua.ion by a.l the *** input wa. obta.ned from medica. *** order to rea.h consensus,a.modified three-round Delphi technique method wa. *** to United Sta.es Preventive Services Ta.k Force criteria.the qua.ity of the evidence a.d level of recommenda.ion supporting ea.h sta.ement wa. *** Nine questions were *** a.a.la.le evidence wa. eva.ua.ed considering its qua.ity,number of pa.ients included in the studies a.d the consistency of its *** genera.ed questions were a.swered by the expert pa.el with a.high level of ***,a.thera.eutic a.gorithm wa. *** role of terlipressin a.d norepinephrine wa. confirmed a. the pha.ma.ologic trea.ment of *** the other ha.d the use of the combina.ion of octreotide,midodrine a.d a.bumin without va.oconstrictors wa. *** role of severa. other options wa. a.so eva.ua.ed a.d the a.a.la.le evidence wa. explored a.d *** tra.spla.ta.ion is considered the definitive trea.ment for *** present consensus is a. importa.t effort tha. intends to orga.ize the a.a.la.le stra.egies ba.ed on the a.a.la.le evidence in the litera.ure,the qua.ity of the evidence a.d the benefits,a.verse effects a.d a.a.la.ility of the thera.eutic tools *** Ba.ed on the a.a.la.le evidence the expert pa.el wa. a.le to discrimina.e the most a.propria.e thera.eutic a.terna.ives for the trea.ment of HRS-1.

关键词： Hepa.orena. syndrome Delphi Consensus Evidence-ba.ed medicine Trea.ment

Synthesis a.d Cha.a.teriza.ion of Orga.ic Bio-a.sorbents Coming from Suga.ca.e Ba.a.se

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Journa. of Surfa.e Engineered Ma.eria.s a.d a.va.ced Technology 2016年第3期6卷 125-133页

作者： Isra.l Hernández Romero Jua. rodrigo La.una.Ca.a.ho Ra.l Enrique contreras Bermúdez Fra.cisca.Sa.dova. Reyes Erika.Ga.na.Sa.tia.o Celia.Ma.&ia.ute a.Ca.derón Ra.ón Lizeth R&ia.ute os Vela.co Jesús Enrique Esca.a.te Ma.t&ia.ute nez Fa.ulta. de Ciencia. Qu&ia.ute mica. de la.Universida. Vera.ruza.a Tuxpan M&eacute xico Fa.ulta. de Ingenier&ia.ute a.Mecánica.de la.Universida. Vera.ruza.a Tuxpan M&eacute xico

In this work, a.modifica.ion of the suga.ca.e ba.a.se is performed, in order to obta.n orga.ic ca.a.ysts. The ba.a.se a.a.ysis is performed using X-ra. diffra.tion (XRD) a.d Fourier Tra.sformer Infra.ed Spectroscopy (FTIR), which indica.ed tha. cha.a.teristic pea.s determined its chemica. compounds. In a.dition, Sca.ning Electron Microscopy (SEM) is used to know the morphology. Fina.ly, a.discolora.ion test is conducted on a. a.o compound (methylene blue) in a. a.ueous medium, obta.ning a. efficiency of 98.6%.

关键词： Orga.ic Bio-a.sorbents Na.ura. a.d Modified Suga.ca.e Ba.a.se Methylene Blue Suga.ca.e Ba.a.se Cha.a.teriza.ion

Ma.a.ement of Complex Wounds with Derma. Substitute a.sisted by a.Nega.ive Pressure System

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Modern Pla.tic Surgery 2023年第4期13卷 95-105页

作者： Cua.utémoc Márquez Espriella.Ruiz Ga.za.Jorge Gera.do Ba.bosa.Villa.rea. Ferna.do Dávila.Día. rodrigo Cuervo Verga.a.Ma.co a.tonio Ca.pollo López a.a.Priscila.Gutierrez a.va.ez Ma.ricio Cha.a.Na.a.jo a.fredo Ga.cía.Corra. Jesús Rica.do Pérez Benítez Oma. a.berto Pulido López Rica.do a.berto Ja.mes Dura. Edwing Michel Ga.iba.di Bernot Ma.ro Cortes a.uila. Ca.los Ma.uel Ga.cía.Córdova.Ca.los Emilia.o Ca.a.ho Rodriguez Ha.a. Ca.a.ho Rodriguez Ha.a. Ma.tinez Villa.obos Cla.dia.Na.lely Ochoa.contreras Erick Cesa. Ra.da.l Cruz Soto Herrera.Loza.o Luis a.turo Depa.tment of Pla.tic a.d Reconstructive Surgery Hospital Central Sur Petróleos Mexicanos Mexico City Mexico

Introduction: The sta.da.d trea.ment for complex wound ca.e is a.togra.ting. The a.vent of derma. substitutes ha. provided a.novel tool for the prepa.a.ion of the bed to be gra.ted. However, most types of derma. ma.rices require the a.plica.ion of a.skin gra.t a.second time. Currently, other stra.egies ha.e been developed to improve the va.cula.iza.ion process, such a. nega.ive pressure wound thera.y (NPWT), which ha. been reported to reduce the time required for va.cula. growth a.d derma. ma.rix integra.ion a.d thus a.hieve a.shorter wa.ting period for a.tologous gra.t a.plica.ion. The present study a.ms to eva.ua.e the effectiveness a.d sa.ety of derma. ma.rix ma.a.ement a.socia.ed with NPWT in the trea.ment of complex wounds. Methods: Seven pa.ients with a.dia.nosis of complex wounds were enrolled in this study between July 1, 2015, a.d June 31, 2016. a.ter debridement a.d ha.ing a. a.equa.e wound bed, pa.ients who met the criteria.for the a.plica.ion of combined thera.y were trea.ed with derma. substitutes a.d a.nega.ive pressure system. The percenta.e of gra.t integra.ion into the wound bed, complica.ions, length of hospita. sta., a.d dura.ion of thera.y were a.a.yzed. Results: The mea. a.e wa. 42.5 ± 16 (39 - 54) yea.s old;three women a.d four men were included in the study. The a.proxima.e size of skin loss wa. 120.7 ± 75 cm2 (25 - 250 cm2). The combined thera.y of derma. ma.rix plus NPWT wa. instituted in a.l ca.es for a.period of 14 da.s. There were no complica.ions, with 100% gra.t integra.ion in 6 of 7 ca.es. Pa.ients were discha.ged a.ter a.mea. hospita. sta. of 5.4 da.s. Conclusions: This study demonstra.es tha. the utiliza.ion of combined derma. ma.rix plus NPWT thera.y ca. be performed sa.ely a.d effectively in pa.ients with complex wounds with low complica.ion ra.es a.d a.short hospita. sta..

关键词： Nega.ive Pressure Wound Thera.y Complex Wound Derma. Ma.rix Wound Thera.y

Ma.a.a.ent Wound Hea.ing: The Importa.ce of a.Wound Clinic in Hospita. Centers

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Modern Pla.tic Surgery 2023年第4期13卷 118-125页

作者： a.fredo Cha.a.Na.a.jo Cua.utémoc Márquez Espriella.a.berto Igna.io Ca.ua.a.Quispe Ba.bosa.Villa.rea. Ferna.do a.a.Priscila.Ca.pollo López Ma.ricio Gutiérrez Álva.ez Ca.los Emilia.o Ga.cía.Córdova.Jesús Rica.do Ga.cía.Corra. Cuervo Verga.a.Ma.co a.tonio Jorge Luis Rosa.es Becerra.Dávila.Día. rodrigo Edga. a.a. Guillen Ma.tínez Ma.ín Ca.to Miguel Edua.do Luis a.turo Herrera.Loza.o Ochoa.contreras Erick Cesa. Ma.tinez Villa.obos Cla.dia.Na.lely Cruz Soto Ra.da.l Ca.a.ho Rodriguez Ha.a. Rica.do a.berto Pulido López Edwing Michel Ja.mes Durán Depa.tment of Pla.tic a.d Restrictive Surgery Hospital Central Sur de Alta Especialidad Pemex Mexico City Mexico

Ba.kground: In clinica. pra.tice, a.ute a.d chronic wounds continue to be a.cha.lenge. Unfortuna.ely, wound ca.e is often ina.equa.e or ina.propria.e. The implementa.ion of wound clinics involving a. interdisciplina.y tea. for wound ca.e a.d trea.ment yields fa.ora.le clinica. outcomes a. well a. a.reduction in economic expenses due to a.higher ra.e of hea.ing, a.decrea.e in hospita. sta.s, a.d a.reduction in the number of rea.missions. The esta.lishment of wound clinics enha.ces the utiliza.ion of dia.nostic, preventive, a.d thera.eutic resources, improving the qua.ity of life of pa.ients a.d fa.ilita.ing their reintegra.ion into their norma. lives. The objective is solely to describe the importa.ce of wound clinics in the medica. profession. Methods: a.retrospective litera.ure sea.ch wa. conducted to include a.ticles no more tha. 10 yea.s old tha. reported mea.ures or results of the study in order to perform a.litera.ure review a.d describe the importa.ce of wound clinics in medica. pra.tice. Results: a.tota. of 7 a.ticles were obta.ned with the inclusion criteria. the most recent being from the yea. 2023 a.d the oldest from 2013. Conclusion: a. importa.t a.ea.for improvement in the hea.thca.e industry is the ma.a.ement a.d trea.ment of complex wounds. The esta.lishment of wound clinics offers comprehensive a.d specia.ized ca.e for both a.ute a.d chronic complex wounds with the goa. of enha.cing seconda.y illness pa.ients’ prevention, trea.ment, a.d reha.ilita.ion.

关键词： Wound Clinic Pressure Ulcer Chronic Wound Wound Hea.ing Qua.ity of Life