检索结果-南通市图书馆

Hydrophobe-substituted bPEI derivatives: boosting transfection on primary vascular cells

Science China Materials 2017年第6期60卷 529-542页

作者： Daniele Pezzoli Eleni K.Tsekoura Remant Bahadur K.C. Gabriele Candiani Diego mantovani Hasan Uludag Laboratory for Biomaterials and Bioengineering CRC- I Department of Mining Metallurgical and Materials Engineering and CHU de Quebec Re- search Centre Laval University Quebec City QC GIV 0A6 Canada Department of Chemical & Material Engineering Faculty of Engineering University of Alberta Edmonton AB T6G 1H9 Canada Department of Chemistry Materials and Chemical Engineering "Giulio Natta" Politecnico di Milano Milan 20131 Italy

Gene therapy targeted to vascular cells repre- sents a promising approach for prevention and treatment of pathological conditions such as intimal hyperplasia, in-stent and post-angioplasty restenosis. In this context, polymeric non-viral gene delivery systems are a safe alternative to viral vectors but a further improvement in efficiency and cytocom- patibility is needed to improve their clinical success. Herein, a library of 24 branched polyethylenimine （bPEI） derivatives modified with hydrophobic moieties was synthesised, char- acterised and tested in vitro on primary vascular cells, aim- ing to identify delivery agents with superior transfection effi- ciency and low cytotoxicity. Low molecular weight PEIs （0.6, 1.2 and 2 kDa） were grafted with long （C18） and short （C3） aliphatic chains, featuring different unsaturation degrees and degrees of substitution. 0.6 kDa bPEI-based derivatives were generally ineffective in transfection on vascular smooth mus- cle cells （VSMCs）, while among the other derivatives some promising vectors were identified. Forcing polyplexes on the cell surface by means of centrifugation invariably boosted transfection levels but increased cytotoxicity as well. Of note, a propionyl-snbstituted derivative （PEI2-PrA1, C3：0） was the most effective on both VSMCs and endothelial cells （ECs）, with higher and more sustained gene expression in combi- nation with markedly lower cytotoxicity with respect to the gold standard 25 kDa bPEI. In addition, a linoleoyl-substi- tuted derivative （PEI1.2-LA6, C18：2） owing to its high effi- ciency in VSMCs and relative inefficacy in ECs, combined with tolerable cytotoxicity was proposed as a vector for spe- cific VSMCs targeting.

关键词： non-viral gene delivery polyethylenimine hy- drophobic substitution smooth muscle cells endothelial cells transfection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Myostatin gene promoter:structure,conservation and importance as a target for muscle modulation

引用

Journal of Animal Science and Biotechnology 2019年第2期10卷 255-273页

作者： Carla Vermeulen Carvalho Grade Carolina Stefano mantovani Lúcia Elvira Alvares Universidade Federal da Integracao Latino-Americana UNILAInstituto Latino-Americano de Ciências da Vida e da NaturezaAvenida Tarquínio Joslin dos Santos1000Foz do IguacuPR CEP 85870-901Brazil Departamento de Bioquímica e Biologia Tecidual Universidade Estadual de Campinas-UNICAMPRua Monteiro Lobato255CampinasSP CEP 13083-862Brazil.

Myostatin(MSTN) is one of the key factors regulating myogenesis. Because of its role as a negative regulator of muscle mass deposition, much interest has been given to its protein and, in recent years, several studies have analysed MSTN gene regulation. This review discusses the MSTN gene promoter, focusing on its structure in several animal species, both vertebrate and invertebrate. We report the important binding sites considering their degree of phylogenetic conservation and roles they play in the promoter activity. Finally, we discuss recent studies focusing on MSTN gene regulation via promoter manipulation and the potential applications they have both in medicine and agriculture.

关键词： CAAT box E-box Gene promoter Myogenesis Myostatin SNP Transcription factors TATA box

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Present Velocity Field in the Italian Region by GPS Data: Geodynamic/Tectonic Implications

引用

International Journal of Geosciences 2015年第12期6卷 1285-1316页

作者： Enzo mantovani Marcello Viti Nicola Cenni Daniele Babbucci Caterina Tamburelli Dipartimento di Scienze Fisiche della Terra e dell’Ambiente Università degli Studi di Siena Siena Italy Dipartimento di Fisica ed Astronomia Università degli Studi di Bologna Bologna Italy

The analysis of geodetic observations carried out by 478 continuous GPS stations in the Italian region since 2001 has allowed a fairly good definition of the ongoing horizontal velocity field with respect to Eurasia. It is argued that such evidence can provide important insights into the geodynamic context in the central Mediterranean area. Numerous velocity vectors in the Apulia zone coherently indicate that the southern Adriatic domain is moving roughly NE ward. Since no significant decoupling zone between this domain and Nubia has so far been recognized, one could expect that the kinematics of these two plates is compatible. However, this condition is not fulfilled if the Nubia-Eurasia relative motion is taken from the global kinematic models, either deduced by long-term evidence?[1]?or short-term geodetic data?[2]?[3]. This problem is considerably reduced if the alternative Nubia-Eurasia rotation pole suggested by?[4]?is taken into account. This choice is also suggested by other major long-term evidence in the Mediterranean region. The numerous geodetic vectors available in two Adriatic sectors, the Apulia zone and the Venetian plain, would imply an Adria-Eurasia rotation pole incompatible with all Nubia-Eurasia Eulerian poles so far proposed. Since a significant relative motion between these plates is not compatible with the absence of a tectonic decoupling zone, we suppose that the short-term kinematics of Adria might be influenced by a transient non-rigid behaviour of that plate. This hypothesis is compatible with the expected effects (post seismic relaxation) of the major decoupling earthquakes that have occurred along Periadriatic zones in the past tens of years. The compatibility of the GPS kinematic pattern in the Apennine belt, Calabria Arc and Sicily with the implications of the geodynamic/tectonic interpretations so far proposed for the central Mediterranean area is then discussed.

关键词： Geodesy Geodynamics Italy Central Mediterranean

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Possible Location of the Next Major Earthquakes in the Northern Apennines: Present Key Role of the Romagna-Marche-Umbria Wedge

引用

International Journal of Geosciences 2017年第11期8卷 1301-1314页

作者： Enzo mantovani Marcello Viti Daniele Babbucci Caterina Tamburelli Nicola Cenni Dipartimento di Scienze Fisiche della Terra e dell’Ambiente Università degli Studi di Siena Siena Italy Dipartimento di Fisica e Astronomia Università degli Studi di Bologna Bologna Italy Dipartimento di Ingegneria Civile Chimica Ambientale e Meccanica Università degli Studi di Bologna Bologna Italy Abstract

It is argued that in some zones of the Northern Apennines, in particular the Rimini-Ancona thrust system, the Romagna Apennines and the Alta Valtiberina trough, the probability of major earthquakes is now higher than in other Apennine zones. This hypothesis is suggested by the comparison of the present short-term kinematics of the Romagna-Marche-Umbria wedge in the Northern Apennines, deduced by the distribution of major shocks in the last tens of years, with the previous repeated behavior of the same wedge, evidenced by the distribution of major earthquakes in the last seven centuries. The seismotectonics of the Apennine region here considered is closely connected with the larger context that involves the progressive migration (from south to north) of seismicity along the peri-Adriatic zones. The information provided by this study can be used to better manage the resources for prevention in Italy.

关键词： Peri-Adriatic Seismicity Northern Apennines Romagna-Marche-Umbria Seismotectonics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Geodynamics of the South Balkan and Northern Aegean Regions Driven by the Westward Escape of Anatolia

引用

International Journal of Geosciences 2023年第5期14卷 480-504页

作者： Enzo mantovani Marcello Viti Daniele Babbucci Caterina Tamburelli Ismail Hoxha Luigi Piccardi Dipartimento di Scienze Fisiche della Terra e dell’Ambiente Università di Siena Siena Italy Institute of Geosciences Polytechnic University of Tirana Tirana Albania CNR-Institute of Geosciences and Earth Resources Florence Italy

The Plio-Quaternary deformation pattern of the northern Aegean and south Balkan regions is interpreted as an effect of the interaction between the Anatolian-Aegean-Pelagonian system (Tethyan belt), undergoing westward extrusion and strong deformation, and the surrounding plates (Nubia, Europe and Adriatic). Since the middle-late Miocene, the collision of the Tethyan belt with the continental Adriatic domain has caused strong E-W shortening in the outer Hellenides and Albanides, also involving the southward extrusion of the Peloponnesus wedge, at the expense of the Ionian oceanic domain. The roughly E-W extension recognized in the western South Balkan zones (Macedonia and eastern Albania) is related to the divergence between the Pelagonian belt (Albanides and Hellenides) and the Rhodope-Moesia domain. Stressed by the westward displacement of the central Anatolian plateau and by the southward bowing of the Cycladic Arc, the northern Aegean zone has contemporaneously undergone E-W compression and N-S extension, which has generated a series of dextral shear faults, delimiting a number of slats. The westward displacement and deformation of such slats can explain the morphological features of the northern Aegean zone. During this phase, the push of the central Anatolian plateau also caused the separation of the Rhodope massif from the Moesian European domain, with the consequent formation of the upper Thrace basin. This hypothesis can explain the Plio-Quaternary compressional deformations recognized in a sector of the North Anatolian fault system, the Ganos-Gelibolu zone. The proposed geodynamic/tectonic interpretation may help to explain some features of the time-space distribution of major earthquakes in the study area.

关键词： South Balkan North Aegean Geodynamics Tectonics Seismicity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Robotic approach with neoadjuvant chemotherapy in adult Wilms’ tumor: A feasibility study report and a systematic review of the literature

引用

Asian Journal of Urology 2023年第2期10卷 128-136页

作者： Simone Sforza Valeria Emma Palmieri Maria Rosaria Raspollini Giandomenico Roviello Alberto mantovani Umberto Basso Maria Carmen Affinita Alberto D’Angelo Lorenzo Antonuzzo Marco Carini Andrea Minervini Lorenzo Masieri Department of Oncologic Minimally-Invasive Urology and AndrologyCareggi HospitalUniversity of FlorenceFlorenceItaly Department of Pediatric Urology Meyer Children HospitalUniversity of FlorenceFlorenceItaly Clinical Oncologic Unit Careggi HospitalUniversity of FlorenceFlorenceItaly Histopathology and Molecular Diagnostics Careggi HospitalUniversity of FlorenceFlorenceItaly Medical Oncology Unit 1 Istituto Oncologico Veneto IOV IRCCSPaduaItaly Hematology Oncology Division Department of Women’s and Children’s HealthPadova University HospitalPaduaItaly Department of Biology and Biochemistry University of BathBathUK

Objective:The incidence of Wilms’tumor(WT)among adult individuals accounts for less than 1%of kidney cancer cases,with a prognosis usually less favorable when compared to younger individuals and an overall survival rate of 70%for the adult patients versus 90%for the pediatric *** diagnosis and treatment of WT are complex in the preoperative setting;neoadjuvant chemotherapy(NAC)or robotic surgery has rarely been *** study aimed to review the literature of robotic surgery in WT and report the first adult WT management using both NAC and robotic ***:We reported a case of WT managed in a multidisciplinary ***,according to Preferred Reporting Items for Systematic reviews and Meta-Analyses recommendations,a systematic review of the literature until August 2020 of WT treated with a robotic approach was carried ***:A 33-year-old female had a diagnosis of *** was scheduled to NAC,and according to the clinical and radiological response to a robotic radical nephrectomy with aortic lymph nodes dissection,she was managed with no intraoperative rupture,a favorable surgical outcome,and a follow-up of 25 months,which did not show any *** systematic review identified a total number of 230 cases of minimally invasive surgery reported in the literature for *** these,approximately 15 patients were carried out using robotic surgery in adolescents while none in ***,NAC has not been administered before minimally invasive surgery in adults up until ***:WT is a rare condition in adults with only a few cases treated with either NAC or minimally invasive approach so *** advantage of NAC followed by the robotic approach could lead to favorable outcomes in this complex ***,additional cases of adult WT need to be identified and investigated to improve the oncological outcome.

关键词： Nephroblastoma Nephrectomy Renal neoplasm Minimally invasive surgery Rare tumor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Short-Term Kinematics of the Adria Plate and Space-Time Distribution of Major Peri-Adriatic Earthquakes

引用

International Journal of Geosciences 2022年第12期13卷 1057-1081页

作者： Enzo mantovani Marcello Viti Daniele Babbucci Caterina Tamburelli Massimo Baglione Vittorio D’Intinosante Dipartimento di Scienze Fisiche della Terra e dell’Ambiente Università di Siena Siena Italy Prevenzione Sismica Settore Sismica della Regione Toscana Italy

Seismic activity is quite strong in the peri-Adriatic zones, whereas the internal part of the Adria plate is almost aseismic. This pattern suggests that Adria is a solid block that interacts with the surrounding belts, trying to move roughly northward. Each major earthquake in a peri-Adriatic zone triggers the acceleration of the decoupled Adria sector, which induces a perturbation of the stress/strain fields in the still blocked boundaries of the plate. Step by step, the displacement of Adria involves more and more northern zones to finally reach the northern front of the plate (eastern Southern Alps). This interpretation seems to be compatible with the time patterns of seismic activity in the main peri-Adriatic zones since 1600 A.D., which may suggest repeated northward migrations of seismic crises. Each supposed migrating sequence involves major earthquakes in most zones. The main features of the first 4 seismic sequences (1600-1930) are used to get insights into possible regularities in the progressive activations of the peri-Adriatic zones. This information and the main features of the ongoing migrating sequence (since 1931) are then used to tentatively recognize the peri-Adriatic zones where the occurrence of next major earthquakes may be most likely.

关键词： Tectonics Adriatic Short-Term Plate Kinematics Seismicity Distribution

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Big Data Analytics for Earth Sciences:the EarthServer approach

引用

International Journal of Digital Earth 2016年第1期9卷 3-29页

作者： Peter Baumann Paolo Mazzetti Joachim Ungar roberto Barbera Damiano Barboni Alan Beccati Lorenzo Bigagli Enrico Boldrini Riccardo Bruno Antonio Calanducci Piero Campalani Oliver Clements Alex Dumitrua,Mike Grant Pasquale Herzig George Kakaletris John Laxton Panagiota Koltsida Kinga Lipskoch Alireza Rezaei Mahdiraji Simone mantovani Vlad Merticariu Antonio Messina Dimitar Misev Stefano Natali Stefano Nativi Jelmer Oosthoek Marco Pappalardo James Passmore Angelo Pio Rossi Francesco Rundo Marcus Sen Vittorio Sorbera Don Sullivan Mario Torrisi Leonardo Trovato Maria Grazia Veratelli Sebastian Wagner Large-Scale Scientific Information Systems Jacobs UniversityBremenGermany Rasdaman GmbH BremenGermany CNR-IIA National Research Council of ItalyInstitute of Atmospheric Pollution ResearchFlorenceItaly EOX IT Services GmbH ViennaAustria Consorzio COMETA CataniaItaly Division of Catania Italian National Institute for Nuclear PhysicsCataniaItaly Department of Physics and Astronomy University of CataniaCataniaItaly MEEO S.r.l. FerraraItaly Plymouth Marine Laboratory PlymouthUK Fraunhofer IGD DarmstadtGermany Athena Research and Innovation Center in Information Communication&Knowledge Technologies AthensGreece British Geological Survey EdinburghUK Software Engineering Italia S.r.l. CataniaItaly British Geological Survey KeyworthUK NASA Ames Research Center Moffett FieldCAUSA

Big Data Analytics is an emerging field since massive storage and computing capabilities have been made available by advanced *** and Environmental sciences are likely to benefit from Big Data Analytics techniques supporting the processing of the large number of Earth Observation datasets currently acquired and generated through observations and ***,Earth Science data and applications present specificities in terms of relevance of the geospatial information,wide heterogeneity of data models and formats,and complexity of ***,Big Earth Data Analytics requires specifically tailored techniques and *** EarthServer Big Earth Data Analytics engine offers a solution for coverage-type datasets,built around a high performance array database technology,and the adoption and enhancement of standards for service interaction(OGC WCS and WCPS).The EarthServer solution,led by the collection of requirements from scientific communities and international initiatives,provides a holistic approach that ranges from query languages and scalability up to mobile access and *** result is demonstrated and validated through the development of lighthouse applications in the Marine,Geology,Atmospheric,Planetary and Cryospheric science domains.

关键词： big data Big Data Analytics array databases Earth Sciences interoperability standards

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

创建宽带数字家庭

引用

今日电子 2001年第7期 28-29页

作者： Alberto mantovani

一、概述目前人们谈论的"宽带数字家庭"更强调的可能是因特网的宽带接入.实际上,这是一个简单化的概念.宽带家庭其实有更广泛的涵义,它不仅包括因特网接入,还包括通过宽带传送到家庭的一整套捆绑服务,如,本地电话、长话、蜂窝电话、寻... 详细信息

一、概述目前人们谈论的"宽带数字家庭"更强调的可能是因特网的宽带接入.实际上,这是一个简单化的概念.宽带家庭其实有更广泛的涵义,它不仅包括因特网接入,还包括通过宽带传送到家庭的一整套捆绑服务,如,本地电话、长话、蜂窝电话、寻呼和娱乐服务,以及在家庭内传送这些服务并确保家庭网免受非法接入的必要技术.面对这一更为丰富的连接环境,线缆和ADSL调制解调器不能再以简单的因特网接入速度判断优劣,而必须以新的标准进行评估.但随之而来的问题是,每种技术能支持多少现有服务?客户如何定购服务?他们如何得到所需的硬件并进行安装?在目前和将来,硬件能在何种程度上支持客户需要的各式各样的捆绑宽带服务?它们能与多功能宽带数字家庭进行天衣无缝的集成吗?

关键词：宽带网 ADSL 通信网

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Present Tectonic Setting and Spatio-Temporal Distribution of Seismicity in the Apennine Belt

引用

International Journal of Geosciences 2015年第4期6卷 429-454页

作者： Enzo mantovani Marcello Viti Daniele Babbucci Caterina Tamburelli Nicola Cenni Massimo Baglione Vittorio D’Intinosante Dipartimento di Scienze Fisiche della Terra e dell’Ambiente Università degli Studi di Siena Siena Italy Dipartimento di Scienze Biologiche Geologiche e Ambientali Università degli Studi di Bologna Bologna Italy Settore Sismica Regione Toscana Firenze Italy

In previous papers, we have argued that a close connection may exist between the discontinuous northward displacement of the Adria plate and the spatio-temporal distribution of major earthquakes in the?periAdriatic?regions?[1]-[3]. In particular, five seismic sequences are tentatively recognized in the post 1400 A.D. seismic history, each characterized by a progressive migration of major shocks along the eastern (Hellenides, Dinarides), western (Apennines) and northern (Eastern Southern Alps) boundaries of Adria. In this work, we describe an attempt at gaining insights into the short-term evolution of the strain field that underlies the migration of seismicity in the Apennine belt. The results of this study suggest that seismicity in the study area is mainly conditioned by the fact that the outer (Adriatic) sector of the Apennine belt, driven by the Adria plate, is moving faster than the inner (Tyrrhenian) belt. This kinematics is consistent with the observed Pleistocene deformation pattern and the velocity field inferred by GPS data. The spatio-temporal distribution of major shocks during the last still ongoing seismic sequence (post 1930) suggests that at present the probability of next major shocks is highest in the Northern Apennines. Within this area, we suggest that seismic hazard is higher in the zones located around the outer sector of the Romagna-Marche-Umbria units (RMU), since that wedge is undergoing an accelerated relative motion with respect to the inner Apennine belt. This hypothesis may also account for the pattern of background seismicity in the Northern Apennines. This last activity might indicate that the Upper Tiber Valley fault system is the most resisted boundary sector of the RMU mobile wedge, implying an higher probability of major earthquakes.

关键词： Seismotectonics Seismic Hazard GPS Apennines Mediterranean

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：