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Genotyping ex situ trees of Abies nebrodensis translocated from the original Sicilian population to enrich the gene pool

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Journal of Forestry Research 2023年第4期34卷 1095-1106页

作者： Anna De Rogatis Fulvio Ducci Serena Guerri Angela Teani roberta proietti CREA Research Centre for Agriculture and EnvironmentVia di Saliceto 8040123 BolognaItaly CREA Research Centre for Forestry and WoodViale Santa Margherita 8052100 ArezzoItaly Comprehensive Institute Venturino Venturi of Loro Ciuff enna Arezzo Via Genova 1252024 Loro Ciuff enna(AR)Italy

As a dynamic ex situ conservation strategy,a clonal seed orchard was started in a nursery in Pomaio(POM)in central Italy in 1993 for an assisted migration experiment of Abies nebrodensis(Lojac.)*** artifi cial ex situ populations were planted with this gene pool:a seedling arboretum in Pieve Santo Stefano(PSS)and a small dendrological collection in Papiano(PAP),both originating from the Sicilian relict ***,using AFLP markers,we estimated the relatedness among the relocated genotypes of the three collections to check whether the three collections had suffi cient genetic variability to be considered as additional sources of variability to the original gene pool for the assisted migration *** individual genetic variability was found in the collections;each plant had a diff erent genotype and was confi rmed to belong to its population of *** and PSS trees were shown to be only from the original population of *** species and were derived from a limited set of maternal fertile *** on the Sicilian fi r population inventory,nursery production in Sicily,and structure clustering analysis,close genetic relationships among POM,PAP and several PSS trees(1–35)were ***,the PSS group(36–78)was genetically close to tree 1 of POM and in a lesser proportion to plants 7 and 9 of *** sampling of seedlings used to form batches in the nursery might have infl uenced the structure of the resultant *** genotypes will be useful for enriching the original gene pool.

关键词： Sicilian fir Genotyping Ex situ conservation Gene pool Assisted migration

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Antisense therapy:a potential breakthrough in the treatment of neurodegenerative diseases

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Neural Regeneration Research 2024年第5期19卷 1027-1035页

作者： roberta Romano Cecilia Bucci Department of Biological and Environmental Sciences and Technologies(DiSTeBA) University of SalentoLecceItaly

Neurodegenerative diseases are a group of disorders characterized by the progressive degeneration of neurons in the central or peripheral nervous ***,there is no cure for neurodegenerative diseases and this means a heavy burden for patients and the health system ***,it is necessary to find new therapeutic approaches,and antisense therapies offer this possibility,having the great advantage of not modifying cellular genome and potentially being *** preclinical and clinical studies aim to test the safety and effectiveness of antisense therapies in the treatment of neurodegenerative *** objective of this review is to summarize the recent advances in the development of these new technologies to treat the most common neurodegenerative diseases,with a focus on those antisense therapies that have already received the approval of the *** and Drug Administration.

关键词： Alzheimer’s disease amyotrophic lateral sclerosis antisense oligonucleotide Huntington’s disease neurodegenerative disorders Parkinson’s disease siRNA

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Oil-in-water nanoemulsions loaded with lycopene extracts encapsulated by spray drying:Formulation,characterization and optimization

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Chinese Journal of Chemical Engineering 2024年第6期70卷 73-81页

作者： Junyang Li roberta Campardelli Giuseppe Firpo Jingtao Zhang Patrizia Perego Department of Civil Chemical and Environmental Engineering(DICCA)University of GenoaVia Opera Pia 15Genova 16145Italy Department of Physics University of GenoaVia Dodecaneso 32Genova 16146Italy School of Food and Bioengineering Zhengzhou University of Light IndustryZhengzhou 450002China

Lycopene is very susceptible to degradation once released from the protective chromoplast *** this study,oil-in-water(O/W)nanoemulsions coupled with spray drying technology were applied for the encapsulation and stabilization of lycopene extracted from tomato *** extract was obtained by ultrasound-assisted *** were prepared by a high-speed rotor stator using isopropyl myristate as the oil phase and Pluronic F-127 as the emulsifier for the aqueous external *** effect of emulsification process parameters was *** drying of the produced emulsions was attempted to obtain a stabilized dry powder after the addition of a coating *** effect of different coating agents(maltodextrin,inulin,gum arabic,pectin,whey and polyvinylpyrrolidone),drying temperature(120-170℃),and feed flow rate(3-9 ml·min^(-1))on the obtained particles was *** revealed that the emulsion formulation of 20/80(O/W)with 1.5%(mass fraction)of Pluronic F-127 as stabilizer in the aqueous phase resulted in a stable nanoemulsion with droplet sizes in the range of 259-276 nm with a unimodal and sharp size *** extract in the nanoemulsion was well protected at room temperature with a degradation rate of lycopene of about 50%during a month of storage *** most stable emulsions were then processed by spray drying to obtain a dry *** drying was particularly successful when using maltodextrin as a coating agent,obtaining dried spherical particles with mean diameters of(4.87±0.17)μm with a smooth *** possibility of dissolving the spray dried powder in order to *** original emulsion was also successfully verified.

关键词： Emulsions Powders Stability Isopropyl myristate Lycopene Spray-drying

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Transanal eco-Doppler evaluation after hemorrhoidal artery embolization

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World Journal of Gastroenterology 2024年第17期30卷 2332-2342页

作者： roberta Tutino Tommaso Stecca Fabrizio Farneti Marco Massani Giulio Aniello Santoro Dipartimento Chirurgia Generale e Specialistica AOU Cittàdella Salute e della Scienza di TorinoTorino 10126Italy Division of General Surgery 1 Treviso Regional HospitalAULSS 2 Marca TrevigianaTreviso 31100Italy Division of Radiology Treviso Regional HospitalAULSS 2 Marca TrevigianaTreviso 31100Italy Tertiary Referral Pelvic Floor Center Division of General Surgery 2AULSS 2 Marca TrevigianaTreviso 31100Italy

BACKGROUND Hemorrhoidal artery embolization(Emborrhoid)is a novel method for the treatment of severe hemorrhoidal *** having a technical success rate of 93%-100%,the clinical success ranges between 63%and 94%,with a rebleeding rate of 13.6%.AIM To evaluate the effectiveness of this procedure in reducing hemorrhoidal flow and hemorrhoidal *** This prospective observational pilot study was conducted at Division of General Surgery 1 and Tertiary Referral Pelvic Floor Center,Treviso Regional Hospital,*** a 2 months period(February-March 2022),consecutive patients with hemorrhoidal bleeding scores(HBSs)≥4,Goligher scores of II or III,failure of non-operative management,and a candidate for Emborrhoid were *** ultrasound with eco-Doppler was performed preoperatively and 1 month after the *** primary endpoint was to quantify the changes in arterial hemorrhoidal flow after *** secondary endpoint was to evaluate the correlation between the flow changes and the *** Eleven patients underwent *** overall pretreatment mean systolic peak(MSP)was 14.66 cm/*** highest MSP values were found in the anterior left lateral(17.82 cm/s at 1 o’clock and 15.88 cm/s at 3 o’clock)and in the posterior right lateral(14.62 cm/s at 7 o’clock and 16.71 cm/s at 9 o’clock)quadrants of the anal *** treatment,the overall MSP values were significantly reduced(P=0.008)although the correlation between MSP and HBS changes was weak(P=0.570).A statistical difference was found between distal embolization compared with proximal embolization(P=0.047).However,the coil landing zone was not related to symptoms improvement(P=1.000).A significant difference in MSP changes was also reported between patients with type 1 and type 2 superior rectal artery(SRA)anatomy(P=0.040).No relationship between hemorrhoidal grades(P=1.000),SRA anatomy(P=1.000)and treatment outcomes was *** The preliminary findings of this pilo

关键词： Hemorrhoidal artery embolization Hemorrhoidal embolization Hemorrhoidal vascularization Transanal eco-Doppler Transanal ultrasound

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Profiles of the Headspace Volatile Organic and Essential Oil Compounds from the Tunisian Cardaria draba (L.) Desv. and Its Leaf and Stem Epidermal Micromorphology

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Phyton-International Journal of Experimental Botany 2024年第4期93卷 725-744页

作者： Wissal Saadellaoui Samiha Kahlaoui Kheiria Hcini Abir Haddada Noomene Sleimi roberta Ascrizzi Guido Flamini Fethia Harzallah-Skhiri Sondes Stambouli-Essassi Laboratory of Biodiversity Biotechnology and Climate Change(LR11-ES09)Faculty of Sciences of TunisUniversity of Tunis El ManarTunis1060Tunisia Laboratory RME-Resources Materials and EcosystemsFaculty of Sciences of BizerteUniversity of CarthageJarzounaBizerte7021Tunisia Dipartimento di Farmacia Universitàdi PisaVia Bonanno 33Pisa56126Italy Laboratory of Bioresources:Integrative Biology and Valorization(LR14-ES06) High Institute of Biotechnology of MonastirUniversity of MonastirMonastir5000Tunisia

In this work, we investigated aroma volatiles emanated by dry roots, stems, leaves, flowers, and fruits of Cardariadraba (L.) Desv. growing wild in Tunisia and its aerial part essential oils (EOs) composition. A total of 37 volatileorganic compounds (96.7%–98.9%) were identified;4 esters, 4 alcohols, 7 hydrocarbons, 12 aldehydes, 5 ketones,1 lactone, 1 organosulfur compound, 2 organonitrogen compounds, and 1 acid. The hydrocarbons form the maingroup, representing 49.5%–84.6% of the total detected volatiles. The main constituent was 2,2,4,6,6-pentamethylheptane(44.5%–76.2%) reaching the highest relative percentages. Forty-two compounds were determined in thetwo fractions of EOs, representing 98.8% and 97.2% of the total oil composition, respectively. The principal componentswere hexadecanoic acid (34.6%), 6-methyl-5-hepten-2-one (18.3%), decanal (15.0%), 6,10,14-trimethyl-2-pentadecanone (13.2%), and n-pentacosane (13%). Micromorphological details of the leaf and stem epidermisusing light microscopy revealed polygonal cells with sinuous walls in the adaxial and abaxial leaf surfaces andnearly rectangular and long ones with linear and thick walls for the stem epidermis. The stomata complexes wereanisocytic in the leaf epidermis and mainly anisocytic and rarely paracytic in the stem epidermis. Non-glandulartrichomes were unbranched and long with an acute apex or short with a convex apex. The glandular ones wereidentified for the first time in this species. They were short-stalked with a large secretory head. The highest stomatalindex (17.02%) was recorded in the abaxial leaf surface. The identification of headspace volatiles and essentialoil compounds can be used to characterize this species, and the various epidermis micromorphologicalfeatures are very useful for biosystematics taxonomic studies within Brassicaceae.

关键词： Cardaria draba aroma profile essential oils epidermis trichomes

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Persicaria lanigera (Polygonaceae) leaf extract exhibits antiulcerogenic and antiproliferative activities against acetic acid-induced ulcerative colitis and cotton pellet-induced granuloma tissue in rats

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Asian Pacific Journal of Tropical Biomedicine 2024年第7期14卷 269-278页

作者： Meshack Antwi-Adjei Ernest Obese Emmanuel Awintiig Adakudugu Benjamin Aboagye Daniel Anokwah Akua Afriyie Karikari roberta Antwi-Adjei Elvis Ofori Ameyaw Department of Pharmacology School of Medical SciencesUniversity of Cape CoastCape CoastGhana Department of Pharmacotherapeutics and Pharmacy Practice School of Pharmacy and Pharmaceutical SciencesUniversity of Cape CoastCape CoastGhana Department of Forensic Sciences School of Biological SciencesUniversity of Cape CoastCape CoastGhana Department of Pharmacognosy and Herbal Medicine School of Pharmacy and Pharmaceutical SciencesUniversity of Cape CoastCape CoastGhana Department of Biomedical Sciences School of Allied Health SciencesCollege of Agriculture and Natural SciencesUniversity of Cape CoastCape CoastGhana

Objective:To assess the effect of leaf extract of Persicaria lanigera on cotton pellet-induced granuloma tissue formation and acetic acid-induced ulcerative ***:Rats were randomly divided into six groups:normal control,negative control,positive control(dexamethasone or sulfasalazine)as well as Persicaria lanigera(100-600 mg/kg)-treated *** effects of the extracts on body weight,antioxidant,and hematological parameters,as well as mast cell proliferation,were *** addition,a histological evaluation was ***:Persicaria lanigera extract significantly decreased the mean exudate amount and suppressed granuloma tissue formation in a concentration-dependent manner in rats(P<0.05).Additionally,the extract significantly increased body weight,improved hematological profile,reduced the disease activity index score and malondialdehyde level,as well as enhanced catalase and superoxide dismutase activities(P<0.05).Histological evaluation showed Persicaria lanigera extract alleviated acetic acid-induced colonic damages,as evidenced by decreased cell necrosis,edema,and inflammatory cell ***:Persicaria lanigera extract possesses antiproliferative,antioxidative,and anti-colitis ***,its underlying mechanisms of action need further investigation.

关键词： Colitis Persicaria lanigera Antioxidant Cotton pellets Mast cells Granuloma tissue formation

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Epidemiology,therapy and outcome of hepatocellular carcinoma between 2010 and 2019 in Piedmont,Italy

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World Journal of Gastrointestinal Oncology 2024年第3期16卷 761-772页

作者： Christian Bracco Marta Gallarate Marco Badinella Martini Corrado Magnino Salvatore D'Agnano roberta Canta Giulia Racca Remo Melchio Cristina Serraino Valentina Polla Mattiot Giovanni Gollè Luigi Fenoglio Department of Internal Medicine Santa Croce e Carle General HospitalCuneo 12100Italy Department of Medical Sciences "City of Health and Science"University HospitalTorino 10100Italy

BACKGROUND Hepatocellular carcinoma(HCC)is the most common primary liver malignancy and the second leading cause of cancer deaths *** is often diagnosed at an advanced stage and therefore its prognosis remains poor with a low 5-year survival *** patients have increasingly complex and constantly changing characteristics,thus up-to-date and comprehensive data are *** To analyze the epidemiology and main clinical characteristics of HCC patients in a referral center hospital in the northwest of Italy between 2010 and *** In this retrospective study,we analyzed the clinical data of all consecutive patients with a new diagnosis of HCC recorded at"Santa Croce e Carle"Hospital in Cuneo(Italy)between 1 January 2010 and 31 December *** highlight possible changes in HCC patterns over the 10-year period,we split the population into two 5-year groups,according to the diagnosis period(2010-2014 and 2015-2019).RESULTS Of the 328 HCC patients who were included(M/F 255/73;mean age 68.9±11.3 years),154 in the first period,and 174 in the *** C virus infection was the most common HCC risk factor(41%,135 patients).The alcoholic etiology rate was 18%,the hepatitis B virus infection etiology was 5%,and the non-viral/non-alcoholic etiology rate was 22%.The Child-Pugh score distribution of the patients was:class A 75%,class B 21%and class C 4%.The average Mayo end-stage liver disease score was 10.6±3.7.A total of 55 patients(17%)were affected by portal vein thrombosis and 158(48%)by portal *** average nodule size of the HCC was 4.6±3.1 cm.A total of 204 patients(63%)had more than one nodule<3,and 92%(305 patients)had a non-metastatic stage of the *** Barcelona Clinic Liver Cancer(BCLC)staging distribution of all patients was:4%very early,32%early,23%intermediate,34%advanced,and 7%*** survival rate was 1.6±0.3 *** 20%of the patients underwent ***,presence of ascites,BCLC stage and therapy w

关键词： Hepatocellular carcinoma Hepatitis virus Alcoholic hepatitis Non-alcoholic fatty liver disease Diagnosis Etiology Treatment

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Cold ischemia time in liver transplantation:An overview

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World Journal of Hepatology 2024年第6期16卷 883-890页

作者： Manuela Cesaretti Alessandro Izzo roberta Anna Pellegrino Alessandro Galli Orestes Mavrothalassitis Department of HPB and Liver Transplantation Brotzu HospitalCagliari 09122Italy Department of Nanophysic Istituto Italiano di TecnologiaGenova 16163Italy Department of Critical Care Medicine and Anesthesia ASST Papa Giovanni XXIIIBergamo 24100Italy Department of Anesthesia and Perioperative Care University of California San FranciscoSan FranciscoCA 94143United States

The standard approach to organ preservation in liver transplantation is by static cold storage and the time between the cross-clamping of a graft in a donor and its reperfusion in the recipient is defined as cold ischemia time(CIT).This simple definition reveals a multifactorial time frame that depends on donor hepatectomy time,transit time,and recipient surgery time,and is one of the most important donor-related risk factors which may influence the graft and recipient’s ***,the growing demand for the use of marginal liver grafts has prompted scientific exploration to analyze ischemia time factors and develop different organ preservation *** review details the CIT definition and analyzes its different *** also explores the most recent strategies developed to implement each timestamp of CIT and to protect the graft from ischemic injury.

关键词： Cold ischemia time Liver transplantation Organ donation Donation after cardiac death Warm ischemia time Machine perfusion

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Participatory Technique Supporting Environmental Management: A Systematic and Bibliometric Review

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Journal of Environmental Protection 2024年第7期15卷 781-795页

作者： Barbara Rentes Barbosa Fabiana Da Silva Soares Luiz Carlos De Faria Kaline De Mello roberta Averna Valente Department of Environmental Science Federal University of Sao Carlos Sorocaba Brazil Research Institute for the Environment and Livelihoods Charles Darwin University Darwin Australia

The increasing need to manage natural resources sustainably, driven by population growth, requires the simultaneous use of Participatory Techniques (PT) and landscape planning for structured decision-making. We conducted a bibliometric and systematic review to provide an overview of PT usage, identifying evolution in scientific production. We considered the number of publications and citations, prominent journals, and highly cited articles on scientific papers published in the Web of Science database between 1993 and 2023. A total of 415 articles related to PT were identified. After content evaluation, 19 critical articles were selected that underpin the growing combined use of models and indices with PT, enhancing the robustness and credibility of decision-making processes.

关键词： Participation Landscape Planning Decision-Making Process Stakeholders Sustainable Management

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Bioremediation Potential of the Macroalga Ulva lactuca (Chlorophyta) for Ammonium Removal in Elastomer Industry Wastewater

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Advances in Bioscience and Biotechnology 2024年第6期15卷 325-343页

作者： Diego Lelis Alex Enrich-Prast Camille R. Chaves Thuane Mendes Anacleto roberta R. C. Pereira Vinicius P. de Oliveira Programa de Ps-Graduao em Biotecnologia Vegetal e Bioprocessos Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil Unidade Multiusurio de Anlises Ambientais Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil Institute of Marine Science Federal University of So Paulo (IMar/UNIFESP) Santos Brazil Department of Thematic StudiesEnvironmental Change and Biogas Solutions Research Center (BSRC) Linkping University Linkping Sweden Department of Marine Biology Institute of Biology Federal University of Rio de Janeiro Rio de Janeiro Brazil

During the production of nitrile rubber, significant amounts of nitrogen in the form of ammonium are generated in the wastewater. The discharge of this high-nitrogen wastewater can lead to serious environmental issues, including eutrophication, disruption of aquatic ecosystems, and groundwater contamination. To mitigate these impacts, this research explored the bioremediation capabilities of the macroalgae Ulva lactuca (Chlorophyta) for removing nitrogen from nitrile rubber production wastewater. The study employed single-phase and Michaelis-Menten decay models based on ammonium consumption, using various dilutions of wastewater to identify the optimal concentration for treatment. The physiological state of the macroalgae was monitored by measuring the photosynthetic capacity and specific growth rate during the experiments. In the presence of U. lactuca, ammonium concentrations decreased in all treatment groups, confirming that the ammonium kinetics conformed to both applied models. Our results show that U. lactuca effectively reduces ammonium concentrations, with an approximate removal rate of 0.020 µM·g−1·min−1 across different wastewater concentrations (70%, 80%, 90%, and 100%). Notably, the treatments with 70%, 80%, and 90% wastewater strength achieved about 67% reduction in ammonium, demonstrating the alga’s capacity to treat high-nitrogen wastewater. The photosynthetic performance of U. lactuca initially declined in control conditions but stabilized across all treatments, highlighting its adaptability. The kinetic analysis using the Michaelis-Menten model indicated a Vmax of 1342 μM·g−1·DMh−1, suggesting a robust capacity for ammonium uptake when fully saturated. Our study underscores the potential of Ulva lactuca as a cost-effective and efficient agent for wastewater bioremediation, particularly in settings with high nitrogen loads.

关键词： Photosynthetic Quantum Yield One-Phase Decay Model Michaelis-Menten Model Nitrogen Physiological Parameters Elastomers

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