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国际城市规划 2011年第2期26卷 124-124页

作者： jan K. Brueckner robert W. Helsley

城市蔓延一方面由城市人口增长和日益增长的生活水平所共同推进。因为这两者均导致了对土地需求的增长；另一方面，快速路建设和机动车拥有率的增长也降低了通勤成本。

关键词：城市蔓延衰落郊区人口增长土地需求交通拥堵市场失灵经济学家

Split-HaloTag imaging assay for sophisticated microscopy of protein–protein interactions in planta

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Plant Communications 2021年第5期2卷 148-159页

作者： Rieke Minner-Meinen jan-Niklas Weber Andreas Albrecht Rainer Matis Maria Behnecke Cindy Tietge Stefan Frank Jutta Schulze Henrik Buschmann Peter Jomo Walla Ralf-R.Mendel robert Hänsch David Kaufholdt Institut für Pflanzenbiologie Technische Universität BraunschweigHumboldtstrasse 138106 BraunschweigGermany Institut für Physikalische und Theoretische Chemie Technische Universität BraunschweigHagenring 30.023c38106 BraunschweigGermany Botany department Universität OsnabrückBarbara Strasse 1149076 OsnabrückGermany Center of Molecular Ecophysiology(CMEP) College of Resources and EnvironmentSouthwest UniversityTiansheng Road No.2Beibei District400715 ChongqingP.R.China

An ever-increasing number of intracellular multi-protein networks have been identified in plant ***-GFP-based protein–protein interaction assays combine the advantages of in vivo interaction studies in a native environment with additional visualization of protein complex *** of their simple protocols,they have become some of the most frequently used ***,standard fluorescent proteins present several drawbacks for sophisticated *** the HaloTag system,these drawbacks can be overcome,as this reporter forms covalent irreversible bonds with synthetic photostable fluorescent *** can be used in adjustable concentrations and are suitable for advanced microscopy ***,we have established the Split-HaloTag imaging assay in plants,which is based on the reconstitution of a functional HaloTag protein upon protein–protein interaction and the subsequent covalent binding of an added fluorescent *** suitability and robustness were demonstrated using a well-characterized interaction as an example of protein–protein interaction at cellular structures:the anchoring of the molybdenumcofactor biosynthesis complex to filamentous *** addition,a specific interactionwas visualized in a more distinctivemannerwith subdiffractional polarizationmicroscopy,Airyscan,and structured illumination microscopy to provide examples of sophisticated ***-GFPand Split-HaloTag can complement one another,as Split-HaloTag represents an alternative option and an addition to the large toolbox of in vivo ***,this promising new Split-HaloTag imaging assay provides a unique and sensitive approach formore detailed characterization of protein–protein interactions using specific microscopy techniques,such as 3D imaging,single-molecule tracking,and super-resolution microscopy.

关键词： advanced microscopy cytoskeleton photostable fluorescent dyes gateway cloning molybdenum cofactor biosynthesis complex protein-protein interaction Split-HaloTag imaging assay

LINE COLORED TREES WITH EXTENDABLE AUTOMORPHISMS

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数学物理学报 1982年第1期 105-110页

作者： Georg Gati,Frank Harary,robert W. Robinson Institut fur Informatik ETH Zurich 5] Austria. Supported by Swiss National Science Foundation Grants Nos. Switzerland. Present address: Institut fur Informatik Universitat Wien Ovrseas Fellow(1980-81) Churchill College University of Cambridge England.Present address: department of Mathematics University of Michigan Ann Arbor MI 48109 U.S.A. University of Newcastle New South Wales 2308 Australia. Temporary address: department of Mathematics University of Michigan Ann Arbor MI 48109 20] U.S.A. until jan.

An E-tree is a rooted line-colored tree such that every automorphism of a subtree contain-s ing the root can be extended to an automorphism of the entire tree. When only one color i used, E-trees correspond both to achiral planted trees and to partitions with successively divisible parts. The exact numbers of E-trees with n points and colors from a store of c are found, with and without the restriction that each color should be used at least one. Asymptotic formulas for these quantities are discussed for c fixed and n→∞.

关键词： tree root LINE COLORED TREES WITH EXTENDABLE AUTOMORPHISMS

PNAS：科学焉已在蝙蝠机体中发现白血病病毒的史前祖先

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现代生物医学进展 2017年第11期17卷 I0002-I0002页

作者： Helena Farkašová[a], Tomáš Hron[a], jan Pačes[b] Pavel Hulva[c],[d] Petr Benda[c],[e] robert James Gifford[f], Daniel Elleder[a], [a]Laboratory of Viral and Cellular Genetics Institute of Molecular Genetics The Czech Academy of Sciences Prague 14220 Czech Republic [b]Laboratory of Genomics and Bioinformatics Institute of Molecular Genetics The Czech Academy of Sciences Prague 14220 Czech Republic [c]department of Zoology Charles University Prague 12844 Czech Republic [d]department of Biology and Ecology University of Ostrava Ostrava 71000 Czech Republic [e]department of Zoology National Museum (Natural History) Prague 11579 Czech Republic [f]MRC-University of Glasgow Centre for Virus Research Glasgow G12 8TA United Kingdom

近日,一项刊登在国际杂志PNAS上的研究报告中,来自格拉斯哥大学和捷克科学院的研究人员通过研究在蝙蝠的基因组中发现了引发罕见类型白血病的病毒家族的古老DNA痕迹。

关键词：白血病病毒科学院蝙蝠祖先机体研究人员 DNA 基因组

自动体外除颤器在院外心脏骤停第一应答者中的应用:前瞻性对照研究

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英国医学杂志中文版 2004年第3期7卷 190-190页

作者： Anouk P van Alem Rob H Vrenken Rien de vos jan G P Tijssen Rudolph W Koster 杨靓

目的：验证警察及消防员使用自动体外除颤器可以提高院外心脏骤停患者出院率的假说。

关键词：自动体外除颤器心脏骤停临床试验院外急救

第二代佳洁士电动牙刷与手动牙刷对牙菌斑去除功效的比较

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牙体牙髓牙周病学杂志 2003年第7期13卷 400-403页

作者： jan Haun Karen Williams Lynn Friesen Anita Ferrante Patricia A. Walters robert D. Bartizek Aaron R. Biesbrok 王漪涛密苏里州大学堪萨斯市牙科学院宝洁公司健康护理研究中心北京宝洁公司佳洁士口腔护理研究院北京100084

目的 :研究新一代电动牙刷 -佳洁士SpinBrushProTM与两种先进设计的手动牙刷 -欧乐 -BCrossAction和高露洁Navigator的去除菌斑功效。方法 :本研究采用随机对照、检查者单盲、九循环交叉设计 ,为期 2个月。受试者分别使用电动牙刷和两... 详细信息

目的 :研究新一代电动牙刷 -佳洁士SpinBrushProTM与两种先进设计的手动牙刷 -欧乐 -BCrossAction和高露洁Navigator的去除菌斑功效。方法 :本研究采用随机对照、检查者单盲、九循环交叉设计 ,为期 2个月。受试者分别使用电动牙刷和两种对照牙刷各 3次。使用Turesky等改良的Quigley -Hein指数 ,在刷牙前后记录菌斑值。结果 :共 12 7名受试者完成实验。佳洁士SpinBrushProTM的菌斑去除值较高露洁Navigator和欧乐 -BCrossAction分别高 4 9%和 2 8% ,结果具有统计学意义 (P <0 .0 0 1)。结论 :佳洁士SpinBrushProTM的菌斑去除功效优于高露洁Narigator和欧乐 -B -CrossAction的菌斑去除功效。

关键词：电动牙刷手动牙刷菌斑去除

口罩对听力受损的人可能是致命的迫切需要创新,以保证在医疗机构中人们能清晰沟通

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英国医学杂志中文版 2020年第12期23卷 687-688页

作者： Chodosh Joshua Barbara E Weinstein jan Blustein 吴庆武(译) 郑瑞(译) 杨钦泰(审校) Division of Geriatrics and Palliative Care in the department of Medicine New York University Grossman School of MedicineNew YorkNYUSA department of Population Health New York University Grossman School of MedicineNew YorkNY VA New York Harbor Healthcare System New YorkNY Audiology Program Graduate CenterCity University of New YorkNY robert F Wagner Graduate School of Public Service New York UniversityNew YorkNY 中山大学附属第三医院耳鼻咽喉头颈外科和变态反应(过敏)科

新型冠状病毒肺炎(COVID-19)的大流行确实改变了医疗的面貌:临床医生、护士、其他工作人员以及患者都戴上了口罩。我们试图在保持所需距离的同时进行交流。由于口罩遮住了面孔,我们无法看清对日常交流至关重要的面部表情和嘴唇动作。

关键词：临床医生医疗机构听力受损面部表情口罩新型冠状病毒日常交流护士

国际糖尿病足工作组:指南的发展和方法学——《国际糖尿病足工作组:糖尿病足防治国际指南(2019)》的一部分

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感染．炎症．修复 2019年第4期20卷 238-243页

作者： Sicco A.Bus Jaap J.van Netten jan Apelqvist robert J.Hinchliffe Benjamin A.Lipsky Nicolaas C.Schaper 无严同(译) 许樟荣(审校) Amsterdam UMC Department of Rehabilitation MedicineAcademic Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands School of Clinical Sciences Queensland University of TechnologyBrisbaneAustralia Diabetic foot clinic Department of SurgeryZiekenhuisgroep TwenteAlmelo and HengeloThe Netherlands department of Endocrinology University Hospital of MalmoMalmoSweden Bristol Centre for Surgical Research University of BristolUK department of Medicine University of WashingtonSeattleUSA Green Templeton College University of OxfordUK Div.Endocrinology MUMC+CARIM and CAPHRI InstituteMaastrichtThe Netherlands International Working Group on the Diabetic Foot(IWGDF) 四川省成都市第三人民医院四川成都 610031 战略支援部队特色医学中心北京 100101

糖尿病足不仅给患者带来巨大的折磨,还给社会带来沉重的经济负担。本指南是以目标为导向,以循证医学为依据,且在全球得到合理实施。践行国际糖尿病足病指南有良好的经济-效益比。国际糖尿病足工作组(IWGDF)从1999年起开始发布和更新国... 详细信息

糖尿病足不仅给患者带来巨大的折磨,还给社会带来沉重的经济负担。本指南是以目标为导向,以循证医学为依据,且在全球得到合理实施。践行国际糖尿病足病指南有良好的经济-效益比。国际糖尿病足工作组(IWGDF)从1999年起开始发布和更新国际指南。IWGDF在着眼于相关临床问题和结局、严谨的文献系统评价、最大可能给予明确而清晰的推荐及其依据的基础上发布了2019年版指南更新。相关推荐均基于推荐评估和评价系统(GRAde)。本文阐述《IWGDF:糖尿病足防治国际指南(2019)》(简称《IWGDF指南》)的制定过程。《IWGDF指南》中指南共6个部分,分别由6个国际专家组编写,从预防、减压、周围动脉病变、感染、创面愈合干预及糖尿病足溃疡分级6个方面提出推荐意见。IWGDF编辑委员会分别从上述6个方面制定了一系列的实践指南,每条推荐意见均经过IWGDF编辑委员会成员和相关领域国际专家的深入评估。我们相信,只要相关专业人员遵循2019版IWGDF指南推荐,并将这些推荐与本地具体情况相结合,就能够进一步改善糖尿病足的预防和治疗水平,同时也会减轻糖尿病足给全世界患者和社会所带来的负担。

关键词：指南发展推荐评估和评价系统国际糖尿病足工作组系统评价循证糖尿病足足溃疡

国际糖尿病足工作组:糖尿病足防治实践指南——《国际糖尿病足工作组:糖尿病足防治国际指南(2019)》的一部分

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感染．炎症．修复 2019年第3期20卷 131-139页

作者： Nicolaas C.Schaper Jaap J.van Netten jan Apelqvist Sicco A.Bus robert J.Hinchliffe Benjamin A.Lipsky 无周秋红许景灿(译) 许樟荣(审校) Div.Endocrinology MUMC+CARIM and CAPHRI InstituteMaastrichtThe Netherlands Amsterdam UMC Department of Rehabilitation MedicineAcademic Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands School of Clinical Sciences Queensland University of TechnologyBrisbaneAustralia Diabetic foot clinic Department of SurgeryZiekenhuisgroep TwenteAlmelo and HengeloThe Netherlands department of Endocrinology University Hospital of MalmoSweden Bristol Centre for Surgical Research University of BristolBristolUK department of Medicine University of WashingtonSeattleUSA Green Templeton College University of OxfordOxfordUK International Working Group on the Diabetic Foot(IWGDF) 中南大学湘雅医院湖南长沙410008 战略支援部队特色医学中心北京100101

糖尿病足给全球糖尿病患者以及卫生保健系统带来沉重的负担。国际糖尿病足工作组(The International Working Group on the Diabetic Foot,IWGDF)自1999年起一直致力于制定基于循证的糖尿病足防治指南。2019年,在文献系统综述以及全球... 详细信息

糖尿病足给全球糖尿病患者以及卫生保健系统带来沉重的负担。国际糖尿病足工作组(The International Working Group on the Diabetic Foot,IWGDF)自1999年起一直致力于制定基于循证的糖尿病足防治指南。2019年,在文献系统综述以及全球多学科领域专家推荐意见的基础上,对IWGDF的所有指南进行了更新。本实践指南在IWGDF指南第六部分的基础上阐述了糖尿病足的预防、分类和治疗的基本原则。同时,阐述了如何基于这些原则从组织层面成功地预防与治疗糖尿病足,并提供了用于辅助糖尿病足筛查的附录(见附录1和附表1)。本实践指南针对的是全球参与糖尿病患者医疗照护的医务人员。全球大量的研究证实,实施这些防治原则与降低糖尿病相关的下肢截肢发生率有关。我们希望这些更新的实践指南能继续作为有效参考文件以帮助卫生保健人员减轻全球糖尿病足的负担。

关键词：糖尿病足足溃疡指南贯彻预防治疗

Is Hypothyroidism Overlooked in Cardiac Surgery Patients?

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Open Journal of Thoracic Surgery 2012年第2期2卷 29-35页

作者： Aarne Jyrala robert E. Weiss Robin A. Jeffries Gregory L. Kay Heart Institute Department of Cardiothoracic Surgery Good Samaritan Hospital Los Angeles Heart Institute Department of Cardiothoracic Surgery Good Samaritan Hospital Los Angeles USA School of Public Medicine Department of Biostatistics University of California Los Angeles USA

Objectives: to analyze adequacy of preoperative thyroid screening of cardiac surgery patients (pts) with hypothyroidism (HT) and compare with pts without HT by demographic data, EuroSCORE (ES) scores, early and late outcomes. Patients: From 1000 cardiac surgery pts from jan 1999 through May 2000 pts with HT were identified (Group 1, n = 80). 920 pts (Group 2) had no HT. Results Group 1 pts were older (p 60 years (p = 0.004), more females (p dequate laboratory tests. 15 (18.8%) had no tests and 18 (22.5%) inadequate tests. 10 (12.5%) pts had no replacement therapy. There was no operative mortality in Group 1 and 14 (1.5%) in Group 2 (p = 0.70). Hospital mortality was higher in Group 1 (6/7.5% vs 37/4.5%), p = 0.03. Stay in postoperative intensive care unit and hospital were similar (p = 0.66 and 0.53). More pts in Group 1 needed prolonged ICU and LOS (p < 0.0001 for both). Occurrence of postoperative AF was higher in Group 1, p < 0.02. Seventeen pts (23.0%) were not discharged home in Group 1 and 87 (10.2%) in Group 2, (p = 0.002). Follow-up mortality was higher in Group 1 (45/58.1% vs 378/43.5%, p = 0.02). Conclusions HT is overlooked in cardiac surgery patients. Long-term mortality is higher in pts with HT. Resource utilization is higher in pts with HT.

关键词： Hypothyroidism Cardiac Surgery Outcome Differences