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一种检测小鼠转录因子活性的微阵列芯片的构建及应用

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生物化学与生物物理进展 2009年第2期36卷 244-253页

作者：孙义民曾令琴张岩魏丽 k R MITCHElSON 张亮程京清华大学医学系统生物学研究中心清华大学医学院生物芯片北京国家工程研究中心博奥生物有限公司

转录因子是一类具有序列特异性的双链DNA结合蛋白.在哺乳动物细胞中,一个转录因子可以调控多个靶基因,一个基因也同时受到多个转录因子的调控,从而形成复杂的基因表达网络调控机制.在蛋白质水平检测特定状态下组织或细胞中的转录因子活... 详细信息

转录因子是一类具有序列特异性的双链DNA结合蛋白.在哺乳动物细胞中,一个转录因子可以调控多个靶基因,一个基因也同时受到多个转录因子的调控,从而形成复杂的基因表达网络调控机制.在蛋白质水平检测特定状态下组织或细胞中的转录因子活性对深入研究基因表达调控的分子机制具有重要的意义.针对TRANSFAC数据库提供的226个小鼠转录因子的PSSM,设计了240个转录因子结合探针,构建了可以在蛋白质水平同时检测约200个小鼠转录因子的微阵列芯片,实验结果显示:a.针对含有不同DNA结合结构域的7个转录因子,进行依赖于抗体的转录因子活性检测,结果证明该芯片具有良好的特异性;b.针对NFκB转录因子纯品,芯片的检测灵敏度可以达到0.5nmol/l,线性范围为0.5～20nmol/l,表明芯片具有较高的灵敏度和良好的定量能力;c.针对经典的Ikk,JNk信号通路和JAk/STAT信号通路,该芯片可以检测到其关键转录因子的活性变化,证明芯片的可靠性;d.针对小鼠前脂肪细胞系3T3-l1的分化,该转录因子活性芯片不但检测到了已经报道的活性发生变化的转录因子,如PPAR家族和C/EBP家族外,还发现了以前没有报道过的SRF等,充分显示了芯片技术的高通量优势和发现新数据的能力.

关键词：基因表达调控转录因子微阵列芯片 TF—ElISA

GeXP多重基因表达遗传分析系统应用于人乳头瘤病毒的分型检测

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中华预防医学杂志 2013年第12期47卷 1163-1163页

作者： Yang M J luo l Nie k 王佶不详中国疾病预防控制中心病毒病所中心实验室

本文建立了一种基于GeXP多重基因表达遗传分析系统的单管多重PCR方法，用于人乳头瘤病毒（HPV）的分型检测。以11种HPV亚型为研究对象，其中包括9种高危亚型（HPV16，18，31，33，35，39，52，58，66）和2种低危亚型（HPV6和11）。体系... 详细信息

本文建立了一种基于GeXP多重基因表达遗传分析系统的单管多重PCR方法，用于人乳头瘤病毒（HPV）的分型检测。以11种HPV亚型为研究对象，其中包括9种高危亚型（HPV16，18，31，33，35，39，52，58，66）和2种低危亚型（HPV6和11）。体系中的引物包括11对亚型特异性的嵌合引物和一对通用引物，保证了各个目的片段具有相对一致的扩增效率。该方法特异性好，

关键词：人乳头瘤病毒分型检测遗传分析基因表达多重系统 HPV亚型通用引物

肺静脉隔离术后残留医源性房间隔缺损风险是否被低估?

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世界核心医学期刊文摘（心脏病学分册） 2006年第12期2卷 12-13页

作者： Hammerstingl C. lickfett l. Jeong k.-M. 罗亮 Department of Medicine-Cardiol-ogy University of Bonn Bonn Germany Dr.

Background: According to present knowledge, pulmonary vein isolation(PⅥ) bears a low interventional risk and has a high feasibility. For completion of PⅥ, left atrial access is achieved via single or double transseptal puncture. We sought to determine the incidence and echocardiographic characteristics of persistent iatrogenic atrial septal defect(iASD) after PⅥ. Further objectives were to define clinical and periprocedural risk factors for the development of iASD. Methods: Every patient admitted for PⅥ at our hospital was screened for eligibility for study participation. Exclusion criteria were inability for undergoing transesophageal echocardiography, preexisting atrial septal defect, open-heart surgery or another transseptal procedure during the follow-up period. Transesophageal echocardiography was performed before PⅥ and after 9 months. Interatrial shunt was characterized by echocardiographic parameters; right-to-left shunting(RlS) was quantified by contrast echocardiography. Results: Forty-two patients were included, 27 patients underwent PⅥ with single transseptal puncture and additional advancement of a second electrophysiologic catheter(group A), 15 patients underwent PⅥ with double transseptal puncture(group B). In 8 patients of group A, iASD persisted after the follow-up period, including 6 patients with distinct RlS. We saw no iASD in group B(P=.011, CI-0.79 to-0.11). Preprocedural pulmonary artery pressure was significantly higher in patients with iASD and accompanying RlS, compared with patients with iASD and no evidence of RlS(23.75±0.50 vs 17.59±5.82, P=.048, CI 0.048-12.27). Conclusion: This is the first study that demonstrates a high incidence of long-term persistent iatrogenic atrial septal defect with RlS after PⅥ. All interatrial shunts occurred after single transseptal puncture with passage of 2 electrophysiologic catheters into the left atrium. Increased preprocedural pulmonary artery pressure seems to promote the occurrence of RlS across iASD

关键词：房间隔缺损先天性心脏病肺静脉隔离医源性

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全球航空业洞见:2017年全行业绩效报告

空运商务 2017年第8期 14-15页

作者： l.E.k.丹.麦科阿兰.刘易斯滕勇

全球航空业曾因无法获得足够回报,长期备受诟病。但继2015年创纪录地收获250亿美元经济利润后,2016年该行业连续第二年翻盘,实现大约170亿美元的经济利润。l.E.k.（艾意凯）咨询对企业经济利润的定义是去除资本成本后的税后营业收入盈余... 详细信息

全球航空业曾因无法获得足够回报,长期备受诟病。但继2015年创纪录地收获250亿美元经济利润后,2016年该行业连续第二年翻盘,实现大约170亿美元的经济利润。l.E.k.（艾意凯）咨询对企业经济利润的定义是去除资本成本后的税后营业收入盈余,我们将经济利润作为衡量一家航空公司随着时间的推移是否有能力满足股东财务要求的重要指标。虽然股东总回报也具有洞察性,但该数据在不同起止日期表现不定,

关键词：全球航空起止日期资本成本第二年商务舱航业科技投入落后者传统认知竞争形态

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连续油管电缆安装系统的开发

焊管 2015年第10期38卷 65-68页

作者：杨社民 NEWMAN k R HAVER N A STONE l R 中国石油集团东南亚管道有限公司北京100028

开发出了一个可以缠绕在卷筒上的连续油管内注入、注出电力电缆安装系统。介绍了该安装系统的油田需求、开发此系统所用理论、该安装系统的各部分装置以及应用案例。该电缆安装系统工作压力为51.72 MPa(7 500 psi),与以前的安装方法相比... 详细信息

开发出了一个可以缠绕在卷筒上的连续油管内注入、注出电力电缆安装系统。介绍了该安装系统的油田需求、开发此系统所用理论、该安装系统的各部分装置以及应用案例。该电缆安装系统工作压力为51.72 MPa(7 500 psi),与以前的安装方法相比,大大降低了电缆安装成本,完全满足在较长连续油管内安装电缆的需求。

关键词：连续油管电缆安装系统开发

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粘滞指进对裂缝导流能力的影响

国外油田工程 1997年第12期13卷 20-23页

作者： D.S.Pope l.k.leung 谢滨 J.Gulbis 四川石油管理局井下作业处

理解裂缝导流能力损害的原因极其重要，它有利于优化压裂设计，获得最大的经济效益，准确分析油气井生产动态。一般地，压裂液对裂缝导流能力的损害包括压裂液残渣、裂缝中残留压裂液的影响、压裂液敏感性损害等。本文将讨论裂缝中粘滞... 详细信息

理解裂缝导流能力损害的原因极其重要，它有利于优化压裂设计，获得最大的经济效益，准确分析油气井生产动态。一般地，压裂液对裂缝导流能力的损害包括压裂液残渣、裂缝中残留压裂液的影响、压裂液敏感性损害等。本文将讨论裂缝中粘滞指进的影响，介绍裂缝中瓜胶压裂液破胶后。残留压裂液对保留渗透率的影响，建立支撑裂缝保留渗透率的预测方法。

关键词：粘滞指进裂缝导流能力渗透率压裂

俄罗斯kurile-kamchatka地区安山质熔体的成分、挥发分组成和微量元素

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岩石学报 2007年第1期23卷 93-104页

作者： M.l.Tolstykh V.B.Naumov A.D.Babansky Vernadsky Institute of Geochemistry and Analytical Chemistry Russian Academy of Sciencesul.Kosygina 19Moscow119991 Russia Institute of Geology of Ore Deposits PetrographyMineralogyand GeochemistryRussian Academy of SciencesStaromonetnyi per.35Moscow119017Russia

Melt inclusions in minerals from some volcanoes of the kurile-kamchatka region were *** studied basaltic andesites and andesites were sampled from volcanoes of the Central kamchatka depression(Shiveluch and Bezymyannyi),Eastern kamchatka volcanic belt(Avachinskii and karymskii),and Iturup Island,Southern kuriles(kudryavyi).Basalts of the 1996 eruption of the karymskii volcanic center and dacites of Dikii Greben'volcano,Southern kamchatka were also *** than 260 melt inclusions from 31 rock samples were homogenized,and quenched glasses were analyzed using electron and ion *** compositions of melt inclusions in andesitic phenoerysts vary in silica contents from 56 to 80wt%.Al_2 O_3 ,FeO,MgO,CaO decrease and Na_2O and k_2O increase with increasing SiO_*** inclusions(about 80% )are dacitic or ***,the compositions of silicic glasses(>65wt% SiO_2)in andesites significantly differ in TiO2,FeO,MgO,CaO,and k_2O contents from those in dacites and ***-potassium melts(k_2O 3.8～6.8wt% )with various SiO_2 from 51.4 to 77.2wt% were found in minerals of all volcanoes *** indicates a contribution of a component selectively enriched in potassium to magmas of the whole region.A great compositional diversity of melt inclusions in plagioelase phenocrysts from the Bezymyannyi andesites suggests a complex history of plagioclase crystallization and magma evolution in the andesite *** from different volcanoes strongly vary in volatile *** highest H_2O contents are found in the melts from Shiveluch(3.0～7.2wt%,4.7wt% on average)and Avachinskii (4.7～4.8wt%);while those are lower in melts of kudryavyi(0.1～2.6wt% ),Dikii Greben'(0.4～1.8wt%),and Bezymyannyi (lorine contents are also *** lowest values are found in the Bezymyannyi melts(0.09wt% on average),the highest Cl contents are typical of melt inclusions in minerals from the karymskii andesites(0.26wt% on average).The melts from Avachinski

关键词：俄罗斯kurile-kamchatka地区安山质熔体成分挥发分组成微量元素

Profile of People living with HIV after 24 Weeks of Treatment with Dolutegravir in kinshasa

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Advances in Infectious Diseases 2023年第4期13卷 660-674页

作者： Berry I. Bongenya Benoit O. kabengele Marie-Thérèse A. S. Sombo Guy M. M. Bumoko Hippolyte N. T. Situakibanza Fridolin k. k. kodondi Gauthier k. Mesia Mariano M. lusakibanza Jean Marie N. kayembe Baudouin B. Buassa Richard l. kalala Erick N. kamangu Faculty of Medicine Bel Campus Technological University Kinshasa Democratic Republic of Congo Focus HIV/AIDS Research Group Kinshasa Democratic Republic of Congo Department of Internal Medicine Faculty of Medicine University of Kinshasa Kinshasa Democratic Republic of Congo Department of Neurology Faculty of Medicine University of Kinshasa Kinshasa Democratic Republic of Congo Biochemistry laboratory Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Clinical Pharmacology Unit Department of Pharmacology Faculty of Medicine and Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Service of Molecular Biochemistry Department of Basic Sciences Faculty of Medicine University of Kinshasa Kinshasa Democratic Republic of Congo

Introduction: The sixth month appointment (M6) is crucial because at the start of the ART course, it is an indicator of the prognosis of the evolution of care and decision-making on the continuity of treatment. Objective: The objective of this study is therefore to present the profile of People living with HIV under treatment with Dolutegravir 6 months after starting ART in kinshasa. Methods: The present study is a cross-sectional view at M6 of a prospective cohort to determine the profile of People living with HIV (PlHIV) after 6 months of ARV Treatment (ART) in kinshasa, DRC. During the M6 appointment, from April to August 2022, a sample of 5 ml of blood was taken for the various analyzes from all HIV patients included. The collection of sociodemographic data as well as biological and clinical data was carried out under the same conditions as at inclusion. The parameters recorded during M6 were: age, sex, and religion, level of study, marital status, profession, socio-economic level, height, weight, Body Mass Index (BMI), clinical profile, opportunistic infections as well as biochemical and molecular assessment. Results: In M6, 62 patients were registered including 38 women (61.3%), thus giving a sex ratio of 1.58 in favor of women. Fifty-seven (57) patients did not respond to the appointment, representing a loss rate of 47.89%. The most common age group is between 36 and 45 years old with 16 patients (26.7%). The mean age was 42.4 ± 13.3 years. The mean weight was 60.5 ± 15.4 kg with a mean BMI of 22.6 ± 5.8 kg/m2. Thirty-four (34) patients (61.82%) were in Clinical Stage 3. Thirty-six (36) patients (67.92%) had a normal clinical condition. The most common opportunistic infections among patients in M6 were: skin pruritus (25.8%), dermatitis (22.6%) and rash (21%). The mean values of biochemical parameters of patients in M6 were within normal ranges. The median Vl value was 2.92 log10 RNA copies/ml with 17.75% of patients experiencing major failure of f

关键词： Profile PlHIV 6 Months of ART Dolutegravir kinshasa

A　CONSTITUTIVE　THEORY　FOR　THE　REYNOlDS－STRESS　ClOSURES　IN　TURBUlENCE　MODEllING

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Journal of Hydrodynamics 1996年第1期8卷 103-109页

作者： l. Y. Tao,(Department of Mechanical Engineering, University of Pittsburgh, PA15261,USA)G. Q. Chen(Centre for Environmental Sciences, Peking University, Beijing 100871,P. R. China)k. R. Rajagopal(Department of Mechanical Engineering, University of Pittsbur

For the Reynolds-stress closures in turbulence modelling, a constitutive theory is developed based on the principle of frame indifference and the theory of invariants. The present study sheds light on existing closure... 详细信息

关键词： turbulence modelling Reynolds-Stress constitutive theory