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World Journal of Gastroenterology 1998年第4期4卷 22-25页

作者： WANG XingWang and XU BinKeywords oxalysine liver neoplasms fetoprotein tumor cell, cultured rna, messenger gene expression in situ hybridization immunohistochemistry

AIM To investigate alpha-fetoprotein (AFP) mrna expression in BEL-7404 human hepatoma cells and the effect of L-4-oxalysine (OXL) on the *** BEl-7404 human hepatoma cells were maintained in RPMI 1640 media. Human AFP cDNA probe was labelled with digoxigenin-11-dUTP by the random primer labelling method. The expression of AFP mrna in Bel-7404 cells was determined by an in situ hybridization technique with digoxigenin-labelled human AFP cDNA probe. The positive intensities of AFP mrna in cells were analyzed by microspectrophotometer and expressed as absorbance at 470nm. For the experiment with OXL, cells were incubated with various concentrations of the agent for *** Essentially all the hepatoma cells contained AFP mrna in the cytoplasm, although in various amounts. The specificity of the hybridization reaction was confirmed by control experiments in which the use of rnase-treated BEL-7404 cells, non-AFP-producing cells (HL-60 human leukemia cells) or a nonspecific cDNA probe resulted in negative hybridization. When the cells were treated with OXL (25, 50mg/L), the content of AFP mrna in the cytoplasm was decreased with the inhibition percentages of 34.3% and 70.1%, respectively (P＜0.05).CONCLUSION AFP mrna was expressed in BEL-7404 human hepatoma cells and OXL suppressed AFP mrna expression in the cells.

关键词： fetoprotein hepatoma rna messenger

Two novel gastric cancer-associated genes identified by differential display

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World Journal of Gastroenterology 1998年第4期4卷 62-64页

作者： YOU Han, XIAO Bing, CUI DaXiang, SHI YongQuan and FAN DaiMingKeywords stomach neoplasms gene clone nucleotides sequence analysis in situ hybridization polymerase chain reaction rna, messenger

AIM To clone novel gastric cancer-associated genes and investigate their roles in gastric cancer *** A method called differential display was used which allows the identification of differentially expressed genes by using PAGE to display PCR-amplified cDNA fragments between gastric cancer cells and normal gastric mucosa cells. These fragments were cloned into plasmid vector pUC18. Homology analysis was made after sequencing these *** Two novel genes were identified compared with sequences from GenBank. One was registered with the AD number AF 051783. In situ hybridization showed that these two novel genes expressed specifically in gastric cancer *** The two novel genes obtained by differential display were confirmed to be gastric cancer-associated genes using in situ hybridization.

关键词： differential identified genes display Two cancer-associated

Antipredator Behavioral Responses of Native and Exotic Tadpoles to Novel Predator

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Asian Herpetological Research 2015年第1期6卷 51-58页

作者： Fang ZHANG Juan ZHAO Yujie ZHANG Kevin messenger Yong WANG College of Life Sciences Anhui Normal University Department of Biological and Environmental Sciences Alabama Agricultural and Mechanical University

Factors related to the invasion process, such as high abundance of invaders, residence time, and functional distinctiveness, are well documented, but less attention has been given to the effects of antipredator strategy of invasive species during colonization. In this study, we explored the antipredator strategy of an introduced species by comparing the predator avoidance behaviors of two native anuran species and one introduced （“exotic”） species in the presence of different predators. The two native anuran species used in the study were Black-spotted Pond FrogRana nigromaculata and Terrestrial Frog Rana limnocharis. The introduced （invasive） species used was American bullfrogLithobates catesbeianus. Chinese pond turtleChinemys reevesii, Red-backed rat snakeElaphe rufodorsata, and Big-headed turtle Platysternon megacephalum were used as predator species. Chinese pond turtles and Red-backed rat snakes are native predators of Black-spotted Pond Frogs and Terrestrial Frogs, while Big-headed turtles are novel （“unfamiliar”） to the two frogs. All three predator species are novel （“unfamiliar”） to the American bullfrog. The results show that tadpoles of the two native species displayed behaviors of recognizing the two native predators, but did not display the capability of identifying the novel predator. Results from our study also suggest that American bullfrog tadpoles exhibited strong antipredator behavioral responses by displaying the capability of identifying “unfamiliar” predators without cohabitation history and prior exposure to them. Such antipredator behavioral responses could have resulted in more favorable outcomes for an invading species during the invasive introductory process.

关键词： tadpoles Bullfrog antipredator responses chemical cues novel predator

Reclassification of Oligodon ningshaanensis Yuan, 1983(Ophidia:Colubridae) into a New Genus, Stichophanes gen. nov. with Description on Its Malacophagous Behavior

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Asian Herpetological Research 2014年第3期5卷 137-149页

作者： Xiaohe WANG Kevin messenger Ermi ZHAO Chaodong ZHU Chengdu Institute of Biology Chinese Academy of Sciences Department of Biological and Environmental Sciences Alabama A and M University Institute of Zoology Chinese Academy of Sciences

The complete mitochondrial cytb gene and the partial nuclear c-mos gene of Oligodon ningshaanensis Yuan, 1983 were sequenced and used for reconstructing the phylogenetic relationship of this taxon amongst alethinophidian snakes. Its strong affinity to the New World subfamily Dipsadinae and the Old World species Thermophis baileyi was inferred. Hemipenial morphology found by authors conflicts with the original description and its similarity with those of the dipsadid snakes is in accordance with our molecular results. Feeding tests show that O. ningshaanensis is a malacophagous predator, which is another matchless character for this species. This behavior is described and compared with other known slug- and snail-feeding snakes. The discovery of the particular position of our subject indicates that erecting a new genus is necessary accommodate this unique species.

关键词： Stichophanes gen. nov. ningshaanensis Dipsadinae molecular phylogeny hemipenial morphology,mollusks -predator

湖北神农架国家级自然保护区两栖爬行动物物种多样性

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四川动物 2009年第2期28卷 286-291页

作者：杨林森 Kevin messenger 廖明尧湖北神农架国家级自然保护区管理局湖北十堰442421 美国北卡罗来那州立大学

对神农架自然保护区两栖爬行动物进行调查，整理了神农架自然保护区两栖爬行动物名录共71种，新发现湖北省新分布种6种，其中腾格里蛙Rana tenggerensis Zhao，MaceyetPapenfuss、宁陕小头蛇Oligodon ningshaanensis Yuan离原分布区较... 详细信息

对神农架自然保护区两栖爬行动物进行调查，整理了神农架自然保护区两栖爬行动物名录共71种，新发现湖北省新分布种6种，其中腾格里蛙Rana tenggerensis Zhao，MaceyetPapenfuss、宁陕小头蛇Oligodon ningshaanensis Yuan离原分布区较远，神农架新分布种3种，神农架两栖爬行动物的区系特征以东洋界（74．69％）为主，广布种（18．31％）、古北界（7％）种类次之，体现了南北混杂的特点。随着海拔的升高，两栖爬行动物生物多样性总体呈下降趋势，400～1600m两栖爬行动物生物多样性最高，随海拔下降的趋势不明显，1900m以上多样性急剧下降。

关键词：两栖爬行动物生物多样性神农架

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狂犬病死亡的出现模式及病毒传染性的增加

中国人兽共患病学报 2003年第5期 135-136页

作者： Sharon L. messenger ,徐国英

Impact of the Miocene orogenesis on Kaloula *** and implication of local refugia on genetic diversification

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Integrative Zoology 2022年第2期17卷 261-284页

作者： Siti N.OTHMAN Eggy Triana PUTRI Kevin R.messenger Yoonhyuk BAE Yi YANG Timothy BOVA Thomas REED Hina AMIN Ming-Feng CHUANG Yikweon JANG Amaël BORZÉE Department of Life Sciences and Division of EcoScience Ewha Womans UniversitySeoulRepublic of Korea Department of Biology Faculty of Mathematics and Natural ScienceAndalas UniversityIndonesia Herpetology and Applied Conservation Laboratory College of Biology and the EnvironmentNanjing Forestry UniversityNanjingJiangsuChina Laboratory of Animal Behaviour and Conservation College of Biology and the EnvironmentNanjing Forestry UniversityNanjingJiangsuChina

The phylogeography of the Kaloula genus in East Asia is still poorly *** of the difficulties is the absence of fossils to corroborate molecular dating ***,we examined the mitochondrial structure of Kaloula *** East Asia and focused on the impact of glaciations on the northernmost species:Kaloula *** determined the phylogenetic relationships,molecular dating,and genetic connectivity assessments within the genus from 1211 bp of concatenated mitochondrial 12S and *** relaxed clock analyses reveal the emergence of Kaloula *** ancestor in East and Southeast Asia between the Eocene and Oligocene,c.38.47 Ma(24.69–53.65).The genetic diversification of lineages then increased on the East Asian Mainland during the Lower Miocene,c.20.10(8.73–30.65),most likely originating from the vicariance and radiation triggered by the orogeny of the Qinghai–Tibetan ***,the dispersal towards the North East Asian Mainland during the Upper Miocene drove the population diversification of *** c.9.01 Ma(3.66–15.29).Finally,the central mainland population became isolated following orogenesis events and diverged into *** during the Pliocene,c.3.06 Ma(0.02–10.90).The combination of population genetic and barrier analyses revealed a significant genetic isolation between populations of Kaloula *** with the massive Qinling–Daba Mountain chain located in south-central ***,we highlight a young divergence within the Eastern Mainland population of ***,possibly attributed to refugia in south eastern China from which populations later expanded.

关键词： boreal digging frog East Asia genetic discontinuity Miocene qinling orogeny

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采购绩效评价与评价内容分析

国际市场 2008年第5期 52-53页

作者： Sources messenger

在一个高度全球化和高度竞争的环境下,想要保持企业的高效率运作,保证企业的持续经营,对企业运行的各个方面进行评价是不可或缺的。采购绩效评估,主要是评价采购部门、采购管理人员和专业经理,

关键词：采购绩效评价企业企业管理采购评价采购人员采购价格采购效率评价参数

吡罗克酮乙醇胺盐的蛋白酶抑制作用及与硫酸锌复配的防脱发功效研究

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日用化学工业（中英文） 2023年第4期53卷 390-397页

作者： Ranjit Kaur Bhogal 陈旭斌 David messenger 宫哲慧孙佳凝皮瑛瑛顾佳音联合利华科沃斯研发中心人类生物科学与技术英国贝德福德郡MK441LQ 联合利华上海研发中心美容健康事业部上海200335

蛋白水解机制导致棒状末端发根和毛囊之间的细胞粘附分解是头发脱落的最终步骤。在休止期晚期和脱落期,蛋白酶活性的增加导致大量脱发。利用EnzChek^(TM)蛋白酶试剂盒,基于荧光方法检测了吡罗克酮乙醇胺盐在体外对蛋白酶的抑制作用;并... 详细信息

蛋白水解机制导致棒状末端发根和毛囊之间的细胞粘附分解是头发脱落的最终步骤。在休止期晚期和脱落期,蛋白酶活性的增加导致大量脱发。利用EnzChek^(TM)蛋白酶试剂盒,基于荧光方法检测了吡罗克酮乙醇胺盐在体外对蛋白酶的抑制作用;并对含吡罗克酮乙醇胺盐和硫酸锌的洗发水进行了两组含受脱发困扰的男性和女性参与者的临床防脱发功效测试。结果表明,吡罗克酮乙醇胺盐可以有效抑制蛋白酶的活性,0.5%吡罗克酮乙醇胺盐在体外可使胰蛋白酶的活性减少最高达53%。硫酸锌可以增强低浓度吡罗克酮乙醇胺盐对胰蛋白酶活性的抑制作用。两组临床测试显示,使用含0.5%吡罗克酮乙醇胺盐和0.018%硫酸锌的洗发水4周,8周和12周后,头发密度较使用前均有显著性增加,12周后分别增加2.7根/cm^(2)和7.5根/cm^(2);较空白对照组,在8周和12周后均有显著性提高。由此提示了吡罗克酮乙醇胺盐对于棒状毛发的保留作用和含吡罗克酮乙醇胺盐和硫酸锌洗发水的防脱发功效。

关键词：脱发脱落期毛发密度蛋白酶吡罗克酮乙醇胺盐硫酸锌

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重新解读“功率”的含义

视听技术 2008年第4期 71-74页

作者： Paul messenger 关良

很多消费者都认为"功率"大小是用来衡量放大器好坏的指标之一。而实际上,功率这个词无论是从电子学角度还是从声学角度来讲,都是非常复杂的。下面这篇译文就总结了一些基本的概念,并且提出了一些比较新颖的观点,可供发烧友们参考。

关键词：功率解读放大器消费者电子学发烧友