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Round spermatid injection into human oocytes:a systematic review and meta-analysis

Asian Journal of Andrology 2021年第4期23卷 363-369页

作者： Brent m Hanson taylor P Kohn Alexander W Pastuszak richard t scott jr Philip J Cheng james m hotaling Department of Reproductive Endocrinology and Infertility IVI-Reproductive Medicine Associates of New JerseyBasking RidgeNJ 07920USA Department of Urology The Brady Urological Institute at Johns Hopkins UniversityBaltimoreMD 21287USA Department of Surgery University of Utah Center for Reconstructive Urology and Men's HealthSalt Lake CityUT 84108USA

many azoospermic men do not possess mature spermatozoa at the time of surgical sperm *** study is a systematic review and meta-analysis evaluating outcomes following round spermatid injection(ROSI),a technique which utilizes immature precursors of spermatozoa for *** electronic search was performed to identify relevant articles published through October *** cohort studies in English involving male patients who had round spermatids identified and used for fertilization with human oocytes were *** rate,pregnancy rate,and resultant delivery rate were assessed following ***-analysis outcomes were analyzed using a random-effects *** were extracted from 22 studies involving 1099 couples and 4218 embryo *** fertilization rate after ROSI was 38.7%(95%confidence interval[CI]:31.5%–46.3%),while the pregnancy rate was 3.7%(95%CI:3.2%–4.4%).the resultant delivery rate was low,with 4.3%of embryo transfers resulting in a delivery(95%CI:2.3%–7.7%).the pregnancy rate per couple was 13.4%(95%CI:6.8%–19.1%)and the resultant delivery rate per couple was 8.1%(95%CI:6.1%–14.4%).ROSI has resulted in clinical pregnancies and live births,but success rates are considerably lower than those achieved with mature *** this technique may be a feasible alternative for men with azoospermia who decline other options,couples should be aware that the odds of a successful delivery are greatly diminished and the prognosis is relatively poor.

关键词： azoospermia in vitro fertilization infertility spermatozoa testis

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A comparison of the relative efficiency of ICSI and extended culture with epididymal sperm versus testicular sperm in patients with obstructive azoospermia

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Asian Journal of Andrology 2020年第2期22卷 222-226页

作者： scott J morin Brent m Hanson Caroline R Juneau Shelby A Neal Jessica N Landis richard t scott jr.james m hotaling Reproductive medicine Associates of New Jersey 140 Allen RoadBasking RidgeNJ 07920USA Division of Reproductive Endocrinology and Infertility Department of Obstetrics and GynecologySidney Kimmel Medical College at Thomas Jefferson University833 Chestnut StreetPhiladelphiaPA 19107USA Foundation for Embryonic Competence 140 Allen RoadBasking RidgeNJ 07920USA Division of Urology Department of SurgeryUniversity of Utah School of Medicine30 N 1900 ESalt Lake CityUT 84132USA Department of Obstetrics and Gynecology University of Utah School of Medicine30 N 1900 ESalt Lake CityUT 84132USA.An abbreviated version of this manuscript was published and presented as an abstract at the American Society for Reproductive Medicine(ASRM)2017 Scientific Congress.

this is a retrospective cohort study comparing blastocyst transfer outcomes following intracytoplasmic sperm injection utilizing epididymal versus testicular sperm for men with obstructive *** cases at a single center between 2012 and 2016 were *** approach was selected at the surgeon’s discretion and included microepididymal sperm aspiration or testicular sperm *** culture was exclusively utilized prior to *** primary outcome was live birth *** outcomes included fertilization rate,blastulation rate,euploidy rate,and implantation rate.A mixed effects model was ***-six microepididymal sperm aspiration cases and 93 testicular sperm extraction cases were *** live birth rate was equivalent(48.6%vs 50.5%,P=0.77).However,on mixed effects model,epididymal sperm resulted in a greater likelihood of fertilization(adjusted OR:1.37,95%CI:1.05–1.81,P=0.02)and produced a higher blastulation rate(adjusted OR:1.41,95%CI:1.1–1.85,P=0.01).As a result,the epididymal sperm group had more supernumerary blastocysts available(4.3 vs 3,Pthe euploidy rate was no *** rates were no different through the first transfer ***,intracytoplasmic sperm injection following microepididymal sperm aspiration resulted in a greater number of usable blastocysts per ***,the true benefit of epididymal sperm may only be demonstrated via a comparison of cumulative pregnancy rates after multiple transfers from one cohort.

关键词： azoospermia intracytoplasmic sperm injection male infertility testicular sperm extraction

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Review of endoscopic techniques in the diagnosis and management of cholangiocarcinoma

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World Journal of Gastroenterology 2008年第19期14卷 2995-2999页

作者： Katherine Nguyen james t Sing jr scott & White Clinic Texas A&M University Health Science Center College of Medicine

Cholangiocarcinoma is a rare malignancy of the biliary tract. Key factors in determining therapeutic options include knowledge of tumor extent, anatomy and obtaining tissue diagnosis. Endoscopically, there are three modalities available to make the diagnosis of cholangiocarcinoma. these include endoscopic retrograde cholangiopancreatography, endoscopic ultrasound with fine needle aspiration and cholangioscopy. management of cholangiocarcinoma endoscopically is typically confined to stent placement for palliative purposes or as a bridge to surgery. In this article, we will review the endoscopic techniques available for the diagnosis and management of cholangiocarcinoma.

关键词： Cholangiocarcinoma Endoscopic ultrasound Endoscopic cholangiopancreatography Cholangioscopy Diagnosis Hanagement

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