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The ability to manipulate ROS metabolism in pepper may affect aphid virulence

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Horticulture Research 2020年第1期7卷 2720-2732页

作者： Mengjing Sun Roeland E.Voorrips Martijn van Kaauwen richard g.fvisser Ben Vosman Plant Breeding Wageningen University&ResearchP.O.Box 3866700 AJ WageningenNetherlands

Myzus persicae has severe economic impact on pepper(Capsicum)***,we identified two populations of ***,NL and SW,that were avirulent and virulent,respectively on *** accession *** transcriptomics approach used in the current study,which is the first study to explore the pepper−aphid interaction at the whole genome gene expression level,revealed genes whose expression is differentially regulated in pepper accession PB2013071 upon infestation with these *** *** NL population induced ROS production genes,while the SW population induced ROS scavenging genes and repressed ROS production *** also found that the SW population can induce the removal of ROS which accumulated in response to preinfestion with the NL population,and that preinfestation with the SW population significantly improved the performance of the NL *** paper supports the hypothesis that *** can overcome the resistance in accession PB2013071 probably because of its ability to manipulate plant defense response especially the ROS metabolism and such ability may benefit avirulent conspecific aphids.

关键词： metabolism pepper overcome

利用gBSSI和SBD作为“锚”将荧光素酶定位到淀粉粒中效果的比较

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自然科学进展 2006年第6期16卷 679-683页

作者：季勤 Jean-Paul Vincken Krit Raemakers Luc C.J.M.Suurs richard g.f.visser 淮阴师范学院生物系淮安223300 Laboratory of Plant Breeding Department of Plant SciencesWageningen UniversityWageningenThe Netherlands

利用马铃薯淀粉粒结合型淀粉合成酶Ⅰ(gBSSI)作为“锚”,在马铃薯淀粉粒形成时将荧光素酶(LUC)定位到淀粉粒中．并对gBSSI／LUC重组蛋白质在转基因淀粉粒中的定位与积累、重组蛋白质中gBSSI与淀粉粒的亲和性以及荧光素酶的活性等进行了... 详细信息

利用马铃薯淀粉粒结合型淀粉合成酶Ⅰ(gBSSI)作为“锚”,在马铃薯淀粉粒形成时将荧光素酶(LUC)定位到淀粉粒中．并对gBSSI／LUC重组蛋白质在转基因淀粉粒中的定位与积累、重组蛋白质中gBSSI与淀粉粒的亲和性以及荧光素酶的活性等进行了分析．结果表明,gBSSI／ LUC重组蛋白质在马铃薯淀粉粒中获得了特异性表达,但与以微生物的淀粉粒结合结构域(SBD) 作为“锚”的SBD／LUC重组蛋白质相比较,它与淀粉粒的亲和性以及荧光素酶的活性均下降．由此认为,在淀粉粒形成过程中,利用SBD作为“锚”将荧光素酶蛋白质定位到淀粉粒中的效果要优于gBSSI．

关键词：荧光素酶 gBSSI SBD amf马铃著

Phased,chromosome-scale genome assemblies of tetraploid potato reveal a complex genome,transcriptome,and predicted proteome landscape underpinning genetic diversity

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Molecular Plant 2022年第3期15卷 520-536页

作者： genevieve Hoopes Xiaoxi Meng John P.Hamilton Sai Reddy Achakkagari fernanda de Alves freitas guesdes Marie E.Bolger Joseph J.Coombs Danny Esselink Natalie R.Kaiser Linda Kodde Maria Kyriakidou Brian Lavrijssen Natascha van Lieshout Rachel Shereda Heather K.Tuttle Brieanne Vaillancourt Joshua C.Wood Jan Mde Boer Nolan Bornowski Peter Bourke David Douches Herman Jvan Eck Dave Ellis Max J.feldman Kyle M.gardner Johannes C.P.Hopman Jiming Jiang Walter S.De Jong Joseph C.Kuhl richard g.Novy Stan Oome Vidyasagar Sathuvalli Ek Han Tan Remco A.Ursum M.Isabel Vales Kelly Vining richard g.f.visser Jack Vossen g.Craig Yencho Noelle L.Anglin Christian W.B.Bachem Jeffrey B.Endelman Laura M.Shannon Martina V.Stromvik Helen H.Tai Bjorn Usadel C.Robin Buell richard finkers Department of Plant Biology Michigan State UniversityEast LansingMI 48824USA Department of Horticultural Science University of MinnesotaSt.PaulMN 55108USA Department of Plant Science McGill UniversitySainte-Anne-de-BellevueQC H9X 3V9Canada Plant Breeding Wageningen University&ResearchPlant Breeding6708 PB Wageningenthe Netherlands IBg-4 Bioinformatics Forschungszentrum JülichWilhelm Johnen Str52428 JülichGermany Department of Plant Soiland Microbial SciencesMichigan State UniversityEast LansingMI 48824USA Bayer Crop Science WoodlandCA 95695USA Averis Seeds B.V. 9640 AA Veendamthe Netherlands International Potato Center 1895 Avenida La MolinaLimaPeru USDA-ARS ProsserWA 99350USA Agriculture and Agri-food Canada fredericton Research and Development Centre FrederictonNB E3B 4Z7Canada School of Integrative Plant Science Cornell UniversityIthacaNY 14853-1901USA Department of Plant Sciences University of IdahoMoscowID 83844USA USDA-ARS Small Grains and Potato Germplasm ResearchAberdeenID 83210USA HZPC Research B.V. Edisonweg 58501 XG Jourethe Netherlands Department of Crop and Soil Science Oregon State UniversityHermistonOR 97838USA School of Biology and Ecology University of Maine5735 Hitchner Hall OronoME 04469USA Department of Horticultural Sciences Texas A&M UniversityCollege StationTX 77843-2133USA Department of Horticulture Oregon State UniversityCorvallisOR 97331USA Department of Horticultural Science North Carolina State UniversityRaleighNC 27695-7609USA Department of Horticulture University of Wisconsin-MadisonMadisonWI 53706USA Department of Horticulture Michigan State UniversityEast LansingMI 48824USA Institute for Biological Data Science Heinrich Heine UniversityDusseldorf40225 DusseldorfGermany Plant Resilience Institute Michigan State UniversityEast LansingMI 48824USA Present address:genNovation B.V. Wageningenthe Netherlands

Cultivated potato is a clonally propagated autotetraploid species with a highly heterogeneous *** assemblies of six cultivars including two chromosome-scale phased genome assemblies revealed extensive allelic diversity,including altered coding and transcript sequences,preferential allele expression,and structural variation that collectively result in a highly complex transcriptome and predicted proteome,which are distributed across the homologous *** species contribute to the extensive allelic diversity in tetraploid cultivars,demonstrating ancestral introgressions predating modern breeding *** a clonally propagated autotetraploid that undergoes limited meiosis,dysfunctional and deleterious alleles are not purged in tetraploid *** a quarter of the loci bore mutations are predicted to have a high negative impact on protein function,complicating breeder’s efforts to reduce genetic *** StCDf1 locus controls maturity,and analysis of six tetraploid genomes revealed that 12 allelic variants of StCDf1 are correlated with maturity in a dosage-dependent *** of the complexity of the tetraploid potato genome with its rampant structural variation and embedded deleterious and dysfunctional alleles will be key not only to implementing precision breeding of tetraploid cultivars but also to the construction of homozygous,diploid potato germplasm containing favorable alleles to capitalize on heterosis in f1 hybrids.

关键词： potato allele diversity phased assembly polyploid pan-genome wild introgressions

Microbial starch-binding domains are superior to granule-bound starch synthase I for anchoring luciferase to potato starch granules

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Progress in Natural Science:Materials International 2006年第12期16卷 1295-1299页

作者： Jean-Paul VINCKEN Luc C.J.M. SUURS richard g.f. visser Laboratory of Plant Breeding Department of Plant Sciences Wageningen University Wageningen The Netherlands

Microbial starch-binding domains (SBD) and granule-bound starch synthase I (gBSSI) are proteins which are accumulated in potato starch granules. The efficiency of SBD and gBSSI for targeting active luciferase reporter proteins to granules during starch biosynthesis was compared. gBSSI or SBD sequences were fused to the N- or C-terminus of the luciferase (LUC) gene, via an artificial Pro-Thr encoding linker sequence. The genes were introduced into an amylose-free (amf) potato mutant. It appeared that SBD was superior to gBSSI as a targeting sequence, mainly because the luciferase retained higher activity in the SBD-containing fusion proteins than in the gBSSI-containing ones.

关键词： luciferase gBSS I SBD amf potato.

The Bemisia tabaci species complex： Additions from different parts of the world

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Insect Science 2013年第6期20卷 723-733页

作者： Syarifin firdaus Ben Vosman Nurul Hidayati Ence Darmo Jaya Supena richard g.f. visser Adriaan Willem van Heusden Research .Center for Bioresources and Biotechnology Bogor Agricultural University Bogor PT East West Seed Indonesia (EWlNDO) Purwakarta West Java Indonesia Wageningen UR Plant Breeding Wageningen UR Droevendaalsesteeg 1 Wageningen the Netherlands graduate School Experimental Plant Sciences Wageningen UR Droevendaalsesteeg 1 Wageningen the Netherlands

Bemisia tabaci is one of the most threatening pests in many crops. We sequenced part of the mitochondrial cytochrome oxidase I gene from fifty whitefly populations collected in Indonesia, Thailand, India and China. Nineteen unique sequences （haplotypes） of the cytochrome oxidase I were identified in these populations. They were combined with sequences available in databases, resulting in a total of 407 haplotypes and analyzed together with nine outgroup accessions. A phylogenetic tree was calculated using the maximum likelihood method. The tree showed that all groups that were found in previous studies were also present in our study. Additionally, seven new groups were identified based on the new haplotypes. Most B. tabaci haplotypes grouped based on their geographical origin. Two groups were found to have a worldwide distribution. Our results indicate that our knowledge on the species complex around B. tabaci is still far from complete.

关键词： dendrogram haplotype mtCOI species complex whitefly

Analysis of the meiosis in the f_1 hybrids of Longiflorum × Asiatic(LA) of lilies(Lilium) using genomic in situ hybridization

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Journal of genetics and genomics 2008年第11期35卷 687-695页

作者： Shujun Zhou Munikote S. Ramanna richard g.E visser Jaap M. van Tuyl Institute of Landscape College of Agriculture ＆ Biotechnology Zhejiang University Hangzhou 310029 China Plant Breeding Lab Wageningen University P.O. Box 386 6700 AJ Wageningen The Netherlands

Longiflorum and Asiatic lilies of the genus Lilium of the family Liliaceae are two important groups of modem lily cultivars. One of the main trends of lily breeding is to realize introgression between these groups. With cut style pollination and embryo rescue, distant hybrids between the two groups have been obtained. However, the fI hybrids are highly sterile or some of them could produce a small number of 2n gametes, and their BC1 progenies are usually triploids. Dutch lily breeders have selected many cultivars from these BC1 progenies based on their variation. It is presumably suggested that such variation could be caused by intergenomic recombination and abnormal meiosis during gamete formation in f1 hybrids of Longiflorum × Asiatic （LA） hybrids in Lilium. Therefore, the meiotic process of ten f1 LA hybrids was cytologically investigated using genomic in situ hybridization and traditional cytological methods in the present research. The results showed that： at metaphase I, the homoeologous chromosome pairing among different f1 hybrids ranged from 2.0 to 11.4 bivalents formed by homoeologous chromosomes per pollen mother cell （PMC）, and very few multivalents, and even very few bivalents were formed by two chromosomes within one genome rather than homoeologous chromosomes in some PMCs; at anaphase I, all biva- lents were disjoined and most univalents were divided. Both the disjoined bivalents （half-bivalents） and the divided univalents （sister chromatids） moved to the opposite poles, and then formed two groups of chromosomes; because the two resulting half-bivalents retained their axes in the cell undisturbed, many crossover types, including single crossovers, three strand double crossovers, four strand double crossovers, four strand triple crossovers, and four strand multiple crossovers between the non-sister chromatids in the tetrads of bivalents, were clearly inferred by analyzing the breakpoints on the disjoined bivalents. The present investigation not only ex

关键词： Lilium genomic in situ hybridization abnormal meiosis crossover 2n gamete

folate Biofortification of Potato by Tuber-Specific Expression of four folate Biosynthesis genes

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Molecular Plant 2018年第1期11卷 175-188页

作者： Jolien De Lepeleire Simon Strobbe Jana Verstraete Dieter Blancquaert Lars Ambach richard g.f. visser Christophe Stove Dominique Van Der Straeten Laboratory of functional Plant Biology Department of Biology Ghent University K.L. Ledeganckstraat 35 9000 Ghent Belgium Laboratory of Toxicology Department of BioanalysisGhent University Ottergemsesteenweg 460 9000 Ghent Belgium Plant Breeding Wageningen University & Research Droevendaalsesteeg 16700 Wageningen the Netherlands These authors contributed equally to this article.

Insufficient dietary intake of micronutHents, known as ＂hidden hunger＂, is a devastating global burden, affecting two billion people. Deficiency of folates （vitamin B9）, which are known to play a central role in Cl metabolism, causes birth defects in at least a quarter million people annually. Biofortification to enhance the level of naturally occurring folates in crop plants, proves to be an efficient and cost-effective tool in fighting folate deficiency. Previously, introduction of folate biosynthesis genes gTPCHI andADCS, proven to be a successful biofortification strategy in rice and tomato, turned out to be insufficient to adequately increase folate levels in potato tubers. Here, we provide a proof of concept that additional introduction of HPPK/DHPS and/or fPgS, downstream genes in mitochonddal folate biosynthesis, enables augmenta- tion of folates to satisfactory levels （12-fold） and ensures folate stability upon long-term storage of tubers. In conclusion, this engineering strategy can serve as a model in the creation of folate-accumulating potato cultivars, readily applicable in potato-consuming populations suffedng from folate deficiency.

关键词： folate vitamin Bg malnutrition biofortification potato metabolic engineering

High-density SNP-based genetic maps for the parents of an outcrossed and a selfed tetraploid garden rose cross, inferred from admixed progeny using the 68k rose SNP array

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Horticulture Research 2016年第1期3卷 68-75页

作者： Mirjana Vukosavljev Paul Arens Roeland E Voorrips Wendy P C van't Westende g D Esselink Peter M Bourke Peter Cox W Eric van de Weg richard g.f.visser Chris Maliepaard Marinus J M Smulders Wageningen UR Plant Breeding Wageningen University&ResearchWageningenNL-6700 AJThe Netherlands Roath BV EindhovenThe Netherlands Pheno geno Roses D.O.O.Bulevar oslobođenja 65a 21000 Novi SadSerbia

Dense genetic maps create a base for QTL analysis of important traits and future implementation of marker-assisted *** tetraploid rose,the existing linkage maps include<300 markers to cover 28 linkage groups(4 homolog... 详细信息

Dense genetic maps create a base for QTL analysis of important traits and future implementation of marker-assisted *** tetraploid rose,the existing linkage maps includege groups(4 homologous sets of 7 chromosomes).Here we used the 68k WagRhSNP Axiom single-nucleotide polymorphism(SNP)array for rose,in combination with SNP dosage calling at the tetraploid level,to genotype offspring from the garden rose cultivar‘Red New Dawn’.The offspring proved to be not from a single bi-parental *** rose breeding,crosses with unintended parents occur *** developed a strategy to separate progeny into putative populations,even while one of the parents was unknown,using principle component analysis on pairwise genetic distances based on sets of selected SNP markers that were homozygous,and therefore uninformative for one *** of the inferred populations was consistent with self-fertilization of‘Red New Dawn’.Subsequently,linkage maps were generated for a bi-parental and a self-pollinated population with‘Red New Dawn’as the common maternal *** densest map,for the selfed parent,had 1929 SNP markers on 25 linkage groups,covering 1765.5 cM at an average marker distance of 0.9 *** with the strawberry(fragaria vesca)genome was *** ICM1 corresponded to *** pseudochromosome 7(fv7),ICM4 to fv4,ICM5 to fv3,ICM6 to fv2 and ICM7 to *** ICM2 corresponded to parts of *** pseudochromosomes 1 and 6,whereas ICM3 is syntenic to the remainder of fv6.

关键词： breeding linkage dosage

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番茄新品种寿研905的选育

中国蔬菜 2023年第7期 105-107页

作者：于彩云刘欣庆程琳张尚斌魏美甜张娜韩宇睿理查德李传友许勇国家进山东寿光蔬菜种业集团有限公司山东省设施蔬菜分子育种重点实验室山东寿光262700 山东省寿光蔬菜产业集团有限公司农业农村部设施蔬菜种质创新重点实验室山东寿光262700 中国科学院北京100101 北京市农林科学院北京100010 山东省蔬菜工程技术研究中心山东寿光262700

寿研905是以自交系M538为母本、f43为父本杂交选育而成的中早熟、粉果型番茄一代杂种。无限生长型,长势旺盛;果实圆形,果面光滑,着色均匀,单果质量220~250 g,果实硬度中等,耐贮运;高抗番茄黄化曲叶病毒病,抗根结线虫;每667 m^(2)产量9 0... 详细信息

寿研905是以自交系M538为母本、f43为父本杂交选育而成的中早熟、粉果型番茄一代杂种。无限生长型,长势旺盛;果实圆形,果面光滑,着色均匀,单果质量220~250 g,果实硬度中等,耐贮运;高抗番茄黄化曲叶病毒病,抗根结线虫;每667 m^(2)产量9 000 kg左右,适宜山东、山西、河南、河北等地早春和秋延保护地栽培。

关键词：番茄寿研905 一代杂种保护地栽培