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Dermatophytes Species Isolated of HIV-Infected Patients Identified by ITS-RFLP and ITS Region Sequencing from Triangulo Mineiro, Minas Gerais State of Brazil

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Advances in Microbiology 2019年第9期9卷 790-803页

作者： Thatiana Bragine-Ferreira Luiz Sé rgio de Lima-Jú nior Larissa Beatriz Silva Leonardo Eurí pedes Andrade-Silva Delio José Mora Bruno Siqueira Prudente Kennio Ferreira-Paim Fabiana Almeida Araú jo santos Luiz ricardo Goulart Mario Leó n Silva-Vergara Infectious Diseases Unit Internal Medicine Department Triangulo Mineiro Federal University Uberaba Brazil Department of Microbiology Tri&acire ngulo Mineiro Federal University Uberaba Brazil Laboratory of Nanobiotechnology Institute of Biotechnology Federal University of Uberl&acire ndia Uberl&acire ndia Brazil

Dermatomycoses are a group of diseases worldwide distributed and affect skin, hair and nails. Several fungal species can be envolved and keratinophilic fungi belonging to the genera Trichophyton, Microsporum and Epidermophyton are very common. These infections are globally distributed and their incidence has progressively increased. Despite their high incidence, few studies have been carried out in HIV-infected patients, regarding frequency, clinical presentations, and species identification. This study aimed to evaluate some epidemiological and clinical aspects of dermatophytosis in HIV-infected patients and to attempt phenotypical and molecular characteristics of their agents. Of 398 patients included, 306 were HIV-infected. Clinical data and samples of skin, hair and nail lesions were simultaneously obtained. The dermatophytes yielded in culture were phenotypically identified and evaluated by ITS-RFLP and ITS (Internal Transcribed Space) sequencing. Dermatophytes frequency in HIV-infected patients with cutaneous lesions was 11.76% similar to the 15.22% observed in non-HIV individuals. Trichophyton rubrum was the most common isolated species in both groups. Throught ITS-RFLP, 29/32 (90.63%) of dermatophytes isolates were identified at the species level. From the 10 isolates randomly selected for ITS sequencing, seven confirmed the ITS-RFLP results while three Trichophyton sp. were not identified as dermatophytes. The identification of these species in HIV-infected patients is highly desirable in order to improve the knowledge of their frequency, geographical distribution and relation to clinical presentation.

关键词： Dermatophytosis Dermatophytes ITS-RFLP ITS-Sequencing HIV-Infected Patients Trichophyton rubrum

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上颌前牙区即刻种植修复的红白美学评分

中国口腔医学继续教育杂志 2019年第2期22卷 72-78页

作者： Guaracilei Maciel Vidigal Mario Groisman Victor Grover Rene Clavijo Igor Guimaraes Barros Paulinelli santos ricardo Guimaraes Fischer 杨宗澄(译) 徐新(审) 不详山东省口腔医院种植科山东省济南市250000

目的:应用红色美学评分(PES)和白色美学评分(WES)评价上颌前牙即刻种植体与即刻修复的美学效果。材料与方法:回顾性分析患者资料。该评估由两名观察者使用12个评估标准进行。结果:在53名患者(22名男性与31名女性)的53例图像中,红色美学... 详细信息

目的:应用红色美学评分(PES)和白色美学评分(WES)评价上颌前牙即刻种植体与即刻修复的美学效果。材料与方法:回顾性分析患者资料。该评估由两名观察者使用12个评估标准进行。结果:在53名患者(22名男性与31名女性)的53例图像中,红色美学评分PES的均值为8.63±2.4(范围1-14),而白色美学评分WES均值为6.92±1.67(范围,2-10)。近中牙龈乳头得到最大的PES均值为(1.39±0.4)。远端牙龈乳头得到最小的PES均值(0.87±0.54)。牙齿表面得到最大的WES均值为(1.54土0.34)且牙齿形态得到最小的WES均值为(1.25土0.43)。结论:根据PES/WES值评估.针对上颌前牙,配合即刻修复的即刻种植体具有良好的临床美学效果。PES和WES评估值显示出较强的观察者本身差异一致性,但较弱的观察者间差异一致性。

关键词：上颌前牙区美学效果评分种植修复即刻种植体即刻修复牙龈乳头评估标准

Acellular Amniotic Membrane Patch Regeneration for Burn Injury: The Future for Wound Healing

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Journal of Biomedical Science and Engineering 2019年第8期12卷 409-413页

作者： Tayná Dias Kalyane Machado Kátia Renata Antunes Kochla ricardo Cunha Marco André Cardoso Rossana Baggio Simeoni Vinicius Coneglian santos Péricles Varella Gomes Luiz Claudio Sobrinho do Nascimento Maria Elisa Brum do Nascimento Luiz Cesar Guarita-Souza Alessandro Brawerman Julio Cesar Francisco School of Health Sciences Positivo University Curitiba Brazil School of Medical Sciences Pontifical Catholic University of Paraná Curitiba Brazil The Paraná Institute of Technology Curitiba Brazil Gel Lab Michigan State University East Lansing USA

Acellular amniotic membrane (AAM) is a biodegradable and biocompatible natural that has a wide range of applications in the field of pharmaceutics, biomedical, chemical and cosmetics industry. One of the most interesting characteristics of AAM is its antibacterial and anti-inflammatory, anti-angiogenic, anti-fibrosis activity is an attractive approach for treating inflammatory disorders, wounds, and burns. The isolated use of AAM may not be sufficient to produce an adequate anti-inflammatory and antimicrobial effect to fulfill different purposes, has attracted considerable attention that has similar properties to the native tissues. This mini-review offers a focuses on modern aspects of the biomaterials, growth factors, biocompatibility, and acellular matrix which are the basic elements for use in the tissue regeneration and replacement after an injury.

关键词： Skin Regeneration Amniotic Membrane Wound Healing Tissue Engineering

Lipid Accumulation Product: Reliable Marker for Cardiovascular Risk Detection?

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Risk Analysis of the Economic Benefits of Ochroma pyramidale:A Case Study of Forest Planting in Brazil

Open Journal of Epidemiology（流行病学期刊） 2021年第3期

作者： Lidiane Aparecida Vila Pires ricardo José Tofano Sandra Maria Barbalho Claudia Rucco Penteado Detregiachi Marcelo Dib Bechara Viviane Alessandra Capelluppi Tofano Jesselina Francisco dos santos Haber Eduardo Federighi Baisi Chagas Ana Maria Gonçalvez Milla Karina Quesada

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Journal of Agricultural Science and Technology(B) 2018年第7期8卷 444-453页

作者： ricardo Hideaki Miyajima Vitória Castro santos Barreto Paulo Andréde Oliveira Gislaine Cristina Batistela Danilo Simoes Department of Forestry Science Sao Paulo State University(Unesp)School of AgricultureBotucatuSao Paulo 18610-307Brazil Department of Agribusiness State Center for Technological Education Paula SouzaBotucatuSao Paulo 18606-855Brazil Department of Production Engineering Sao Paulo State University(Unesp)Campus of ItapevaSao Paulo 18409-010Brazil

The forest investment projects,analyzed through the application of simulation techniques,allow measuring more accurately the benefits or losses,considering the systemic uncertainties related to forest *** this perspective,the objective of this study was to identify the relative importance of sources of uncertainties in determining the net present value(NPV)of planting of Ochroma pyramidale on a forested property located in the state of S?o Paulo,***,it assigns probability distributions to the parameters with uncertainties and applies the Monte Carlo technique to obtain the stochastic behavior of the NPV,of the modified internal rate of return(MIRR)and the profitability index(PI).The probabilistic results of the quantitative methods analyzed allowed us to conclude that for the planting ***,with 5-year cutting age,indicated economic *** addition,there is a probability that 84.6%of the project will add economic value to the invested capital.

关键词： Balsa wood forest plantation forest management net present value Monte Carlo

Technical Evaluation of Hybrid Clones of Corymbia spp. to Produce Market Pulp

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Study of Wastewater Treatment Plants in Operation with UASB Reactors in the Municipality of Juazeiro do Norte-Ceará

造纸与生物质材料（英文版） 2022年第3期

作者： Marcelo Moreira da Costa ricardo de Carvalho Bittencourt Thales Augusto Pinto Coelho Nogueira Larissa Soares Silva Weslley Henrique Martins da Silva Sebasti?o Renato Valverde Gleison Augusto dos santos Daniel Alexander Fernandes Coelho Claudilene Aparecida Alves Pena

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Journal of Environmental Science and Engineering(B) 2018年第6期7卷 215-223页

作者： Francisco de Assis Martins Ponce Joao Paulo Leite Félix AndréGadelha de Oliveira ricardo Leandro santos Araújo Center of Technological Sciences University of FortalezaFortaleza 60811-905Brazil Department of Civil Construction Federal Institute of EducationScience and Technology of CearáFortaleza 60040-531Brazil

The indiscriminate release of effluents without any type of treatment,or previous treatment without adequate disinfection,can cause several inconveniences for the water body and society,including water-borne *** the present study,the physicochemical and biological aspects of three WWTPs(Wastewater Treatment Plants)operated with UASB(Upflow Anaerobic Sludge Blanket)technology in Juazeiro do Norte were analyzed in order to verify the state of CearáState legislation,compliance with standards for the release of domestic effluents into bodies’*** the research,it verified that the stations did not meet the effluent release standards for TSS(Total Suspended Solids)and removal of Escherichia coli in several analyzed *** also verified that the UASB stations need a post-treatment in order to assist in the process of removal of organic matter and pathogens.

关键词： Effluent UASB analyze

Experimental salt cavern in offshore ultra-deep water and well designevaluation for CO_(2) abatement

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International Journal of Mining Science and Technology 2019年第5期29卷 641-656页

作者： Alvaro Maia da Costa Pedro V.M.Costa Antonio C.O.Miranda Mariana B.R.Goulart Okhiria D.Udebhulu Nelson F.F.Ebecken ricardo C.Azevedo Sérgio M.de Eston Giorgio de Tomi Andre B.Mendes Julio R.Meneghini Kazuo Nishimoto Claudio Mueller Sampaio Camila Brandao Alexandre Breda University of Sao Paulo(USP) Sao Paulo 01000-000Brazil Modecom-Technology in Geomechanics and Computing Modeling Rio de Janeiro 27570-000Brazil Department of Civil and Environment Engineering University of Brasília(UnB)Brasília 70000-000Brazil COPPE Federal University of Rio de JaneiroRio de Janeiro 27570-000Brazil Technology Division of Shell Shell BrazilRio de Janeiro 27570-000Brazil

This paper presents a proposal for an experimental salt cavern in offshore ultra-deep water for CO2 abatement,including the instrumentation plan and well conceptual design evaluated for carbon capture and storage(CCS)*** studies are based on applied computational mechanics associated with field experimentation that has contributed to the technical feasibility of the underground potash mine at the State of Sergipe in *** knowhow allowed the stability analysis of several salt caverns for brine production at the State of Alagoas in Brazil and to the drilling through stratified thick layers of salt of the pre-salt reservoirs in santos ***,this knowledge has been applied in the design of onshore and offshore salt caverns opened by dissolution for storage of natural gas and *** geomechanical study,through the application of computational mechanics,of offshore giant salt caverns of 450 m high by 150 m in diameter,shows that one cavern can store about 4 billion Sm3 or 7.2 million tons of *** the construction of the giant cavern,which will be the first gas storage offshore in the world,it has been decided to develop an experimental one,with smaller size,to obtained field *** experimental cavern will allow the calibration of parameters to be used in the structural integrity analysis of the cavern and well for storage of natural gas which is rich in CO2 under high pressure.

关键词： Salt cavern Pre-salt reservoir Geomechanical study Carbon capture and storage

In situ Colonization of Marine Biofilms on UNS S32760 Duplex Stainless Steel Coupons in Areas with Different Water Qualities： Implications for Corrosion Potential Behavior

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Journal of Marine Science and Application 2014年第3期13卷 346-353页

作者： Luciana V. R. de Messano Barbara L. Ignacio Maria H. C. B. Neves ricardo Coutinho Fundagao Carlos Chagas Filho de Amparo Pesquisa do Estado do Rio de Janeiro (FAPERJ). Av. Erasmo Braga 118/6 andar Centro. CEP: 20020-000. Rio de Janeiro. RJ. Brazil. Present address: lnstituto do Mar Universidade Federal de Sao Paulo. Av. Almirante Saldanha da Gama 89. Ponta da Praia. CEP11030-400. Santos. SP. Brazil. Marine Biotechnology Division Instituto de Estudos do Mar Almirante Paulo Moreira Marinha do Brasil. Rua Kioto 253. Praia dosAnjos. CEP: 28930-000. Arraial do Cabo. RJ. Brazil.

In the presence of biofilms, stainless steels （SS） exhibits an increase in corrosion potential, called ennoblement. In the present study, the corrosion potential （Eco,） behavior of the duplex SS UNS S32760 was recorded simultaneously with the in situ marine biofilm formation in two areas at Arraial do Cabo, Southeastern Brazil. The biofilm at Forno Harbor （an anthropogenically disturbed area） was characterized by higher relative abundances of Bacteria at day 2, followed by diatoms （especially Navicula sp.） on day 10 and dinoflagellates on day 18, whereas no clear trend was recorded at Cabo Frio Island （an undisturbed area）. The ennoblement of Ecor values was site-dependent. In a complementary laboratory assay, biofilms were removed and the Eco values registered in sterile conditions for the subsequent 10 days and corroborated in situ results. Understanding biofilms and SS interactions has important implications for materials science and engineering decisions as well as helping to fill in imnortant gaps in this knowledge.

关键词： duplex stainless steel microbiologically influencedcorrosion corrosion potential ennoblement marine biofilm fieldexperiment