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Protective effects of Amaranthus hybridus against aflatoxin B1 and fumonisin B1-induced genotoxicity in H4IIE-luc cells

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Hepatoma Research 2015年第1期1卷 136-146页

作者： Mohamed I.M.Ibrahim rialet pieters Sekena H.Abdel-Aziem Anna M.vander Walt Cornelius C.Bezuidenhout John P.Giesy Mosaad A.Abdel-Wahhab Food Toxicology and Contaminants Department National Research CenterDokkiCairo 12622Egypt Unit of Environmental Sciences and Management North-West UniversityPrivate Bag X6001Potchefstroom 2520South Africa Cell Biology Department National Research CenterDokkiCairo 12622Egypt Department of Veterinary Biomedical Sciences and Toxicology Centre University of SaskatchewanSaskatoonSaskatchewan S7N 5B4Canada Department of Zoology and Center for Integrative ToxicologyMichigan State UniversityEast LansingMI 48824USA Department of Biology and Chemistry and State Key Laboratory in Marine Pollution City University of Hong KongKowloonHong KongChina School of Biological Sciences University of Hong KongHong KongChina State Key Laboratory of Pollution Control and Resource Reuse School of the EnvironmentNanjing UniversityNanjing 210093JiangsuChina Department of Biology Hong Kong Baptist UniversityHong KongChina

Aim:Protective effects of aqueous extract of Amaranthus hybridus against afl atoxin B1(AFB_(1))and/or fumonisin B1(FB_(1))on the H4IIE-luc cell line were determined by use of the methyl thiazol tetrazolium viability assay and disruption of DNA ***:H4IIE-luc cells were incubated with different concentrations of AFB_(1) and/or FB_(1) for 24 and 48 h with or without aqueous extract of ***:AFB_(1) decreased the viability of cells after 24 and 48 h of ***_(50)values for AFB_(1) were 10.5 and 1.8μmol/L for the two periods,*** the 48 h exposure to mycotoxin repeated with a pre-treatment of 20 and 40μg/mL extract of ***,the EC_(50)changed to 3.88 and 7.67μmol/L,respectively.H4IIE-luc cells exposed to FB_(1) for 24 h responded more than those incubated for 48 *** treated with a combination of AFB_(1) and FB_(1) were less viable with a signifi cant decrease in the greater *** mixture of AFB_(1) and FB_(1) resulted in a signifi cant threat to H4IIE-luc as indicated by the absence or appearance of new bands in random amplifi ed polymorphic DNA analysis,which demonstrated damage to *** protective effects were probably due to greater content of total phenolics,carotenoids,β-carotene,folic-,linolenic-,linoleic and palmitic acids,as well as calcium,magnesium,iron,zinc,and selenium observed in the ***:Exposure to 40μg/mL of extract of *** protected cells from damage to DNA by stabilizing DNA.

关键词： Afl atoxin B1 Amaranthus hybridus cytotoxicity DNA fumonisin B1 hepatoma cells methyl thiazol tetrazolium assay

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Aqueous extract of Corchorus olitorius decreases cytotoxicityof aflatoxin B1 and fumonisin B1 in H4IIE-luc cells

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Hepatoma Research 2015年第1期1卷 75-85页

作者： Mohamed I.M.Ibrahim rialet pieters Sekena H.Abdel-Aziem Anna M.van der Walt Cornelius C.Bezuidenhout John P.Giesy Mosaad A.Abdel-Wahhab Department of Food Toxicology and Contaminants National Research CentreDokkiCairo 12622Egypt Unit of Environmental Sciences and Management North-West UniversityPrivate Bag X6001Potchefstroom 2520South Africa Department of Cell Biology National Research CentreDokkiCairo 12622Egypt Department of Veterinary Biomedical Sciences and Toxicology Centre University of SaskatchewanSaskatoonSaskatchewan S7N 5B4Canada Department of Zoology and Center for Integrative ToxicologyMichigan State UniversityEast LansingMI 48824USA Department of Biology and Chemistry and State Key Laboratory in Marine Pollution City University of Hong KongKowloonHong KongChina School of Biological Sciences University of Hong KongHong KongChina State Key Laboratory of Pollution Control and Resource Reuse School of the EnvironmentNanjing UniversityNanjing 210093JiangsuChina Department of Biology Hong Kong Baptist UniversityHong KongChina

Aim:Afl atoxin B1(AFB1)and fumonisin B1(FB1)are important food-borne ***-contamination of foodstuffs with these two mycotoxins is well-known and has been implicated in a possible development of hepatocellular carcinoma in humans living in regions of the world where exposures to these mycotoxins in grain are *** aim of the current study was to evaluate the potential protective effects of an aqueous extract of Cochorus olitorius(***,moroheiya)against cytotoxicity of AFB1 and/or FB1 in H4IIE-luc rat hepatoma cells,using assays to measure cell viability and disruption of DNA *** this transactivation assay was originally developed to specifi cally respond to aryl hydrocarbon agonists,this cell line was used because of its hepatic ***:H4IIE-luc cells were incubated with different concentrations of AFB1 and/or FB1 for 24 and 48 h with or without aqueous extract of ***:Both mycotoxins decreased cell viability and increased DNA *** was more pronounced when cells were exposed simultaneously to AFB1 and ***:Aqueous extract of *** protected cells against cytotoxicity of *** contains a water-soluble,natural chemo-preventative agent for cancer that should be isolated and identifi ed.

关键词： Anticancer cytotoxicity DNA liver moroheiya mycotoxins

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Modelling the bed characteristics in fluidised-beds for top-spray coating processes

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Particuology 2012年第6期10卷 649-662页

作者： Mike Vanderroost Frederik Ronsse Koen Dewettinck Jan G.pieters Department of Biosystems Engineering Ghent UniversityCoupure links 6539000 GhentBelgium Laboratory of Food Technology and Engineering Ghent UniversityCoupure links 6539000 GhentBelgium

A particle sub-model describing the bed characteristics of a bubbling fluidised bed is presented. Atomisation air, applied at high pressures via a nozzle positioned above the bed for spray formation, is incorporated in the model since its presence has a profound influence on the bed characteristics, though the spray itself is not yet considered. A particle sub-model is developed using well-known empirical relations for particle drag force, bubble growth and velocity and particle distribution above the fluidised-bed surface. Simple but effective assumptions and abstractions were made concerning bubble distribution, particle ejection at the bed surface and the behaviour of atomisation air flow upon impacting the surface of a bubbling fluidised bed, The model was shown to be capable of predicting the fluidised bed characteristics in terms of bed heights, voidage distributions and solids volume fractions with good accuracy in less than 5 min of calculation time on a regular desktop PC. It is therefore suitable for incorporation into general process control models aimed at dynamic control for process efficiency and product quality in top-spray fluidised bed coating processes.

关键词： Fluidised bed Modelling Voidage distribution Fluidised bed characteristics Multiphase flow Coating process

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Bayesian network model to distinguish between intentional attacks and accidental technical failures:a case study of floodgates

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Cybersecurity 2021年第1期4卷 442-460页

作者： Sabarathinam Chockalingam Wolter pieters AndréTeixeira Pieter van Gelder Faculty of Technology Policy and ManagementDelft University of TechnologyDelftThe Netherlands Department of Risk Safety and SecurityInstitute for Energy TechnologyHaldenNorway Behavioural Science Institute Radboud UniversityNijmegenThe Netherlands Department of Electrical Engineering Uppsala UniversityUppsalaSweden

Water management infrastructures such as floodgates are critical and increasingly operated by Industrial Control Systems(ICS).These systems are becoming more connected to the internet,either directly or through the corporate *** makes them vulnerable to *** behaviour in floodgates operated by ICS could be caused by both(intentional)attacks and(accidental)technical *** operators notice abnormal behaviour,they should be able to distinguish between those two causes to take appropriate measures,because for example replacing a sensor in case of intentional incorrect sensor measurements would be ineffective and would not block corresponding the attack *** the previous work,we developed the attack-failure distinguisher framework for constructing Bayesian Network(BN)models to enable operators to distinguish between those two causes,including the knowledge elicitation method to construct the directed acyclic graph and conditional probability tables of BN *** a full case study of the attack-failure distinguisher framework,this paper presents a BN model constructed to distinguish between attacks and technical failures for the problem of incorrect sensor measurements in floodgates,addressing the problem of floodgate *** utilised experts who associate themselves with the safety and/or security community to construct the BN model and validate the qualitative part of constructed BN *** constructed BN model is usable in water management infrastructures to distinguish between intentional attacks and accidental technical failures in case of incorrect sensor *** could help to decide on appropriate response strategies and avoid further complications in case of incorrect sensor measurements.

关键词： Bayesian network DeMorgan model Intentional attack Probability elicitation Safety Security Technical failure Water management

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Decision support model for effects estimation and proportionality assessment for targeting in cyber operations

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Defence Technology（防务技术） 2021年第2期17卷 352-374页

作者： C.Maathuis W.pieters J.van den Berg Delft University of Technology Jaffalaan 52628 BXDelftNetherlands TNO Military Operations Oude Waaldorperweg 632597 AKDen HaagNetherlands Netherlands Defense Academy De la Reyweg 1204818 BBBredaNetherlands Open University of the Netherlands Valkenburgerweg 1776419 ATHeerlenNetherlands

Cyber operations are relatively a new phenomenon of the last two *** that period,they have increased in number,complexity,and agility,while their design and development have been processes well kept under *** a consequence,limited data(sets)regarding these incidents are *** various academic and practitioner public communities addressed some of the key points and dilemmas that surround cyber operations(such as attack,target identification and selection,and collateral damage),still methodologies and models are needed in order to plan,execute,and assess them in a responsibly and legally compliant *** on these facts,it is the aim of this article to propose a model that i))estimates and classifies the effects of cyber operations,and ii)assesses proportionality in order to support targeting decisions in cyber *** order to do that,a multi-layered fuzzy model was designed and implemented by analysing real and virtual realistic cyber operations combined with interviews and focus groups with technical e military *** proposed model was evaluated on two cyber operations use cases in a focus group with four technical e military *** the design and the results of the evaluation are revealed in this article.

关键词： Cyber operations Cyber warfare Cyber weapons Artificial intelligence Fuzzy logic Targeting

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Anti-infective, cytotoxic and antioxidant activity of Ziziphus oxyphylla and Cedrela serrata

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Asian Pacific Journal of Tropical Biomedicine 2016年第8期6卷 671-676页

作者： Rizwan Ahmad Niyaz Ahmad Atta Abbas Naqvi Paul Cos Louis Maes Sandra Apers Nina Hermans Luc pieters Natural Products and Alternative Medicine College of Clinical Pharmacy University of Dammam Department of Pharmaceutics College of Clinical Pharmacy University of Dammam Department of Pharmacy Practice College of Clinical Pharmacy University of Dammam Faculty of Pharmaceutical Biomedical and Veterinary Sciences Laboratory of Microbiology Parasitology and Hygiene (LMPH) University of Antwerp Faculty of Pharmaceutical Biomedical and Veterinary Sciences Natural Products & Food–Research and Analysis (NatuRA)University of Antwerp

Objective: To search for the most active antimicrobial and antioxidant sub-fractions related to traditional use of Ziziphus oxyphylla(Z. oxyphylla) and Cedrela serrata(C. serrata) in Pakistan against infectious and liver ***: Factions of different polarity were tested in vitro for their antiprotozoal,antimalarial, antibacterial and antifungal activity against different pathogens. Cytotoxicity on MRC-5 cell lines(human lung fibroblasts) as well as, in vitro radical scavenging activity was evaluated using the 1, 1-diphenyl-2-picrylhydrazyl radical ***: The highest antiprotozoal activity was observed for the CHCl3 fractions of Z. oxyphylla roots and leaves, and C. serrata bark. The CHCl3 and Et OAc fractions of Z. oxyphylla roots, the CHCl3 fraction of Z. oxyphylla leaves, the Et OAc and the residual Me OH: H2 O fraction of C. serrata bark showed antibacterial activity against Staphylococcus aureus. The same residual Me OH: H2 O fraction of C. serrata bark was active against Candida albicans. The highest antioxidant activity was observed for the more hydrophilic Et OAc fractions of Z. oxyphylla leaves, C. serrata bark and leaves, and the residual Me OH: H2 O fraction of C. serrata ***: This study supports at least in part the traditional uses of these plants for antimicrobial purposes and against liver diseases.

关键词： Antifungal Antibacterial Antiprotozoal Cytotoxicity Antioxidant Ziziphus oxyphylla Cedrela serrata Rhamnaceae Meliaceae Pakistan

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Spectral Compensation for Linear-Logarithmic Flow Cytometry Acquisitions

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Journal of Biomedical Science and Engineering 2014年第9期7卷 631-640页

作者： Nickolaas van Rodijnen Math pieters Marius Nap Atrium Medical Centre Heerlen The Netherlands

Compensating for fluorescence overlap in multiparameter flow cytometry datasets, of which one parameter is linear distributed and at least one parameter is logarithmic distributed, leads usually to extreme high compensation values. We investigated this phenomenon with an adapted flow cytometry model, of which the two parameters can easily be converted from linear to logarithmic and vice versa. With the adapted model, spectral compensation was performed both for linear-logarithmic and linear-linear parameter distribution. The results of the flow cytometry model were validated with a real world example which was also compensated twice. The results of the two experiments show that the compensation values equal to the theoretically expected value when both parameters are linear distributed. However, the compensation value exceeds 100% when one of the two parameters is logarithmic distributed. In addition, we found that spectral compensation of differently distributed parameters leads to deformation of the compensated events. With the adapted flow cytometry model presented in this paper it is shown how to correctly compensate flow cytometry acquisitions with different distributed parameters.

关键词： Flow Cytometry DNA Spectral Compensation

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LHP1 Interacts with ATRX through Plant-Specific Domains at Specific Loci Targeted by PRC2

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Molecular Plant 2018年第8期11卷 1038-1052页

作者： Haifeng Wang Danhua Jiang Elin Axelsson Zdravko J. Lorkovic Sean Montgomery Sarah Holec Bas J.G.E. pieters Abbas H.K. AI Temimi Jasmin Mecinovic Frederic Berger Gregor Mendel Institute (GMI) Austrian Academy of Sciences Vienna Biccenter (VBC) Dr. Bohr-Gasse 3 1030 Vienna Austria Temasek Lifesciences Laboratory 1 Research Link Nationat University of Singapore 117604 Singapore Singapore Department of Biological Sciences National University of Singapore 14 Science Drive 4 117543 Singapore Singapore Institute for Molecules and Materials Radboud University Heyendaalseweg 135 6525 AJ Nijmegen the Netherlands

Heterochromatin Protein 1 （HP1） is a major regulator of chromatin structure and function. In animals, the network of proteins interacting with HP1 is mainly associated with constitutive heterochromatin marked by H3K9me3. HP1 physically interacts with the putative ortholog of the SNF2 chromatin remodeler ATRX, which controls deposition of histone variant H3.3 in mammals. In this study, we show that the Arabidopsis thaliana ortholog of ATRX participates in H3.3 deposition and possesses specific conserved domains in plants. We found that plant Like HP1 （LHP1） protein interacts with ATRX through domains that evolved specifically in land plant ancestors. Loss of ATRX function in Arabidopsis affects the expression of a limited subset of genes controlled by PRC2 （POLYCOMB REPRESSIVE COMPLEX 2）, including the flowering time regulator FLC. The function of ATRX in regulation of flowering time requires novel LHPl-interacting domain and ATPase activity of the ATRX SNF2 helicase domain. Taken together, these results suggest that distinct evolutionary pathways led to the interaction between ATRX and HP1 in mammals and its counterpart LHP1 in plants, resulting in distinct modes of transcriptional regulation.

关键词： ATRX LHPI1 H3 variants Chromatin Histone Evolution

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