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Genetic variation for phytic acid content in mungbean(Vigna radiata L. Wilczek)

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The Crop Journal 2015年第2期3卷 157-162页

作者： Vinod janardan Dhole kandali Srinivasalu reddy Nuclear Agriculture and Biotechnology Division Bhabha Atomic Research Centre

Mungbean(Vigna radiata L. Wilczek) is a short-duration legume crop cultivated for seeds that are rich in protein and carbohydrates. Mungbeans contain phytic acid(PA), an anti-nutritional factor that is the main storage form of organic phosphorus in seeds. It is a strong inhibitor against the absorption of nutrients including iron, zinc, calcium and magnesium in monogastric animals. Genotypes with low phytic acid(lpa) in seed may show increased assimilation of nutrients and be useful in breeding lpa cultivars. The present study was conducted to identify lpa sources, genetic variation, heritability, and association with seed coat color, inorganic phosphorus(IP), and seed size in 102 mungbean genotypes including released varieties, land races, mutants, and wild species grown in two seasons: summer 2011 and rabi 2012. PA and IP in dry seeds were estimated by modified colorimetric method and Chen's modified method,respectively. PA, IP, and 100-seed weight differed significantly in the two seasons. PA content in102 genotypes ranged from 5.74 to 18.98 mg g-1and 5.85 to 20.02 mg g-1in summer 2011 and rabi 2012, respectively. High heritability was found for PA(0.87 and 0.86) and seed size(0.82 and0.83) but low heritability for IP(0.61 and 0.60). A negative correlation was found between PA and seed size(r =-0.183 and-0.267). Yellow and green seed coat genotypes contained significantly less PA than black seed coat genotypes. Cluster analysis revealed the distinctness of wild species, land races and cultivated varieties on the basis of PA content. The genotypes YBSM(6.001 mg g-1) and JL-781(6.179 mg g-1) showed lowest PA. These lpa sources can be used to develop high-yielding mungbean cultivars with low phytic acid.

关键词： Mungbean Phytic acid Inorganic phosphorus Cluster analysis

禁食状态下MED1肝脏特异性敲除鼠呈现高脂血症

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生物工程学报 2011年第10期27卷 1490-1498页

作者：白亮付涛贾玉枝 Jayme Borensztajn janardan k.reddy 杨公社西北农林科技大学动物科技学院动物脂肪沉积与肌肉发育实验室杨凌712100 Department of Pathology Feinberg School of MedicineNorthwestern UniversityChicago IL 60611USA

为研究MED1如何影响血浆脂质代谢,以MED1肝脏特异性敲除鼠(MED1△Liv)为动物模型,禁食0、24、48、72 h,通过H&E染色切片观察了MED1△Liv鼠肝脏的形态学变化,运用甘油三酯和胆固醇酶试剂盒及FPLC方法分析了MED1△Liv和对照鼠(MED1fl/fl)... 详细信息

为研究MED1如何影响血浆脂质代谢,以MED1肝脏特异性敲除鼠(MED1△Liv)为动物模型,禁食0、24、48、72 h,通过H&E染色切片观察了MED1△Liv鼠肝脏的形态学变化,运用甘油三酯和胆固醇酶试剂盒及FPLC方法分析了MED1△Liv和对照鼠(MED1fl/fl)血浆甘油三酯和胆固醇的水平以及脂蛋白的分布情况。研究结果显示,禁食72 h,与MED1fl/fl和PPARα-/-对照鼠相比,MED1△Liv鼠肝脏无脂肪沉积。禁食24、48、72 h,与MED1fl/fl鼠相比,MED1△Liv鼠血浆甘油三酯显著升高。禁食48 h和72 h,与MED1fl/fl鼠相比,MED1△Liv鼠血浆胆固醇显著升高。此外,饲喂条件下,MED1△Liv和MED1fl/fl鼠血浆脂蛋白分布无明显差异。然而,禁食24 h,MED1△Liv鼠中富含甘油三酯的极低密度脂蛋白高度积聚,表明MED1△Liv鼠呈现的高脂血症是由于极低密度脂蛋白的积聚引起,提示转录辅激活子MED1在富含甘油三酯的脂蛋白代谢中起关键调控作用。

关键词： MED1 甘油三酯胆固醇极低密度脂蛋白

高脂饮食诱导肝脏MED1特异性敲除小鼠呈高胆固醇血症

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中国生物化学与分子生物学报 2014年第11期30卷 1113-1118页

作者：李倩薇刘芮菡赵四海贾玉枝白亮 janardan k.reddy 徐小平刘恩岐陕西中医学院陕西咸阳712046 西安交通大学医学部心血管中心脂代谢与动脉粥样硬化研究室西安710061 西安交通大学医学部实验动物中心西安710061 Department of Pathology Feinberg School of MedicineNorthwestern University

为研究肝脏MED1对脂质代谢的影响,以肝脏MED1特异性敲除(MED1ΔLiv)小鼠为模型,对其进行基因型鉴定,H&E染色观察肝脏组织学变化,免疫组织化学染色检测肝脏MED1蛋白表达;高脂饲料(脂肪含量为60%)饲喂小鼠,并分别在0、1、2和4周动态检测... 详细信息

为研究肝脏MED1对脂质代谢的影响,以肝脏MED1特异性敲除(MED1ΔLiv)小鼠为模型,对其进行基因型鉴定,H&E染色观察肝脏组织学变化,免疫组织化学染色检测肝脏MED1蛋白表达;高脂饲料(脂肪含量为60%)饲喂小鼠,并分别在0、1、2和4周动态检测血浆胆固醇和甘油三酯及血糖水平.结果显示,与MED1fl/fl小鼠相比,MED1ΔLiv小鼠仅肝脏MED1 mRNA表达水平显著降低,其它组织表达无明显变化.高脂饲喂1周和2周,MED1ΔLiv小鼠血浆总胆固醇水平显著升高(P<0.01);普通或高脂饲料饲喂状态下,与MED1fl/fl小鼠相比,MED1ΔLiv小鼠血糖水平均显著降低(P<0.05).短期给予高脂饲料可诱导MED1ΔLiv小鼠呈现高胆固醇血症,提示MED1在胆固醇代谢中发挥重要调控作用.

关键词： MED1 肝脏胆固醇血糖