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miR-29a Promotes Lipid Droplet and Triglyceride Formation in HCV Infection by Inducing Expression of SREBP-1c and CAV1

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Journal of Clinical and Translational Hepatology 2016年第4期4卷 293-299页

作者： Mennatallah Mamdouh Mahdy Nada Magdy El-Ekiaby rana mahmoud hashish Radwa Ayman Salah Rasha Sayed Hanafi Hassan Mohamed El-Said Azzazy Ahmed Ihab Abdelaziz Department of Pharmaceutical Chemistry German University in Cairo New Cairo City Egypt Department of Pharmacology and Toxicology German University in Cairo New Cairo City Egypt School of Medicine New Giza University Cairo Egypt Department of Pharmaceutical Biology German University in Cairo New Cairo City Egypt Department of Stem Cells and Regenerative Medicine Zewail City of Science and TechnologyGiza Egypt Department of Chemistry Novel Diagnostic & Therapeutics American University in Cairo New Cairo City Egypt

Aims:To examine the regulation of SREBP-1c and CAV1 by microRNA-29a (miR-29a) in cells infected with hepatitis C virus (HCV) in an attempt to control HCV-induced nonalcoholic fatty liver ***:In order to examine the manipulation of SREBP-1c and CAV1 by miR-29a,oleic acid (OA)-treated JFH-I-infected Huh-7 cells were *** was added 24 h post-transfection and gene expression was investigated by qRT-PCR at 48 h post *** functional impact of the observed alteration in SREBP-1c and CAV1 expression was analyzed by examining lipid droplet (LD) and triglyceride (TG) content at 72 h post-OA treatment using light microscopy and spectrophotometry,*** load was quantified by qRT-PCR at 72 h ***:OA treatment induced the expression of miR-29a and SREBP-1c,as compared to untreated *** miR-29a expression led to a significant up-regulation of SREBP-1c as well as CAV1 compared to mock untransfected *** expression of miR-29a resulted in a marked increase in LDs and their respective TGs,while miR-29a antagomirs decreased both the LD and TG content compared to mock untransfected ***,forcing the expression of miR-29a in JFH-1 HCV-infected Huh-7 cells resulted in 53% reduction in viral titers compared to mock untransfected Huh-7 ***:Inducing miR-29a expression significantly induces SREBP-1c and CAV1 expression,thereby increasing LDs as well as their respective ***,forcing the expression of miR-29a resulted in reduction of HCV RNA levels in Huh-7 cells.

关键词： Caveolin-1 HCV Lipid droplets MicroRNA-29a SREBP-1c

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