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Polytene chromosomes of an Indian Himalayan black fly Simulium (Nevermannia) praelargum (Diptera:Simuliidae)

Current Zoology 2010年第4期56卷 437-444页

作者： Willie HENRY Subrata Kumar DEY rakesh varma Sachin THAPA William S PROCUNIER RG. Department of Zoology Darjeeling Government College Darjeeling West Bengal 734101 India School of Biotechnology West Bengal University of TechnologyBF-142 Sector 1Salt Lake City Kolkata 700064 West Bengal India Consultant North Bay Ontario P1B 5W6 Canada

High quality polytene chromosome maps (n=3) of a Himalayan Simuliid Simulium praelargum Datta, 1973 are presentedand represent the first cytological description of a taxon found in the feuerborni group, subgenus Nevermannia. Polytenechromosomes one (Ⅰ) and two (Ⅱ) are metacentric, chromosome three (Ⅲ) is submetacentric with the length of each chromosomeoccupying 37.25 %, 31.36 % and 31.34 % of the total complement length, respectively. Typical simuliid diagnostic intergenericchromosomal markers are found within the polytene complement of this species. The nucleolar organizer (N.O.) is found at thebase of the short arm of chromosome one (IS), the Ring of Balbiani (R.B.), double bubble (D.B.) and triad occur in the short armof chromosome two (IIS), the Parabalbiani Ring (P.B.) and grey band (gb) occur in the long arm of chromosome two (IIL) and theBlister (BL) and Capsule (Ca) occur in the short arm of chromosome three (HIS).Terminal bands at the end of HIS are heterochromatinizedand present atypically with respect to other simuliid fauna. Populations studied so far are unique among the Simuliidaein that they exhibit chromosome structural monomorphism. These high resolution polytene chromosome maps will form thebasis for future cytological characterization and phylogenetic comparisons amongst members of the feuerborni group.

关键词：多线染色体喜马拉雅双翅目染色体长度细胞学鉴定印度核仁组织者染色体结构

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Value of neutrophil-lymphocyte ratio in evaluating response to percutaneous catheter drainage in patients with acute pancreatitis

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World Journal of Clinical Cases 2022年第1期10卷 91-103页

作者： Pankaj Gupta Gaurav Chayan Das Akash Bansal Jayanta Samanta Harshal S Mandavdhare Vishal Sharma Shano Naseem Vikas Gupta Thakur Deen Yadav Usha Dutta Neelam varma Manavjit Singh Sandhu rakesh Kochhar Department of Radiodiagnosis Postgraduate Institute of Medical Education and ResearchChandigarh 160012India Department of Gastroenterology Postgraduate Institute of Medical Education and ResearchChandigarh 160012India Department of Hematology Postgraduate Institute of Medical Education and ResearchChandigarh 160012India Department of Surgical Gastroenterology Postgraduate Institute of Medical Education and ResearchChandigarh 160012India

BACKGROUND Early prediction of response to percutaneous catheter drainage(PCD)of necrotic collections in acute pancreatitis(AP)using simple and objective tests is critical as it may determine patient *** role of white blood cell(WBC)count and neutrophil-lymphocyte ratio(NLR)has not been assessed as a tool of early prediction of PCD success and is the focus of this *** To assess the value of WBC and NLR in predicting response to PCD in *** This retrospective study comprised consecutive patients with AP who underwent PCD between June 2018 and December *** and fluid collections were classified according to the revised Atlanta classification and organ failure was defined according to the modified Marshall *** and NLR were monitored 24 h prior PCD(WBC-0/NLR-0)and 24 h(WBC-1/NLR-1),48 h(WBC-2/NLR-2)and 72 h(WBC-3/NLR-3)after *** was calculated by dividing the number of neutrophils by the number of *** association of success of PCD(defined as survival without the need for surgery)with WBC and NLR was *** trend of WBC and NLR was also assessed post *** One hundred fifty-five patients[median age 40±13.6(SD),64.5%males,53.5%severe AP]were included in the final *** was done for acute necrotic collection in 99(63.8%)patients and walled-off necrosis in 56(36.1%)*** pain to PCD interval was 24±69.89 *** was successful in 109 patients(group 1)and 46 patients(group 2)who failed to *** was no significant difference in the baseline characteristics between the two groups except the severity of AP and frequency of organ *** WBC and NLR showed an overall decreasing *** was a significant difference between WBC-0 and WBC-1(P=0.0001).WBC-1 and NLR-1 were significantly different between the two groups(P=0.048 and 0.003,respectively).The area under the curve of WBC-1 and NLR-1 for predicting the success of PCD was 0.602 and 0.682,*** a cut-off value of 9.87 fo

关键词： Acute pancreatitis Percutaneous catheter drainage White blood cell Neutrophil-lymphocyte ratio Necrotic collection

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Biodegradation of Styrene-Butadiene-Styrene Coploymer via Sugars Attached to the Polymer Chain

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Advances in Materials Physics and Chemistry 2013年第2期3卷 112-118页

作者： rakesh Singh Rishi Gupta Mukund G. Adsul Ramesh C. Kuhad Digambar V. Gokhale Anjani J. varma Department of Microbiology University of Delhi (South Campus) New Delhi India National Collection of Industrial Microorganisms Biochemical Sciences Division National Chemical Laboratory Pune India Polymer Science & Engineering Division National Chemical Laboratory Pune India

A synthetic method was developed to chemically attach few molecules of simple sugars like glucose, mannose, galactose, maltose and xylose (0.09 - 0.37 wt%) and with quaternary nitrogen pendants (0.42 - 0.46 atomic%) along the polybutadiene section of polystyrene-block-polybutadiene-block-polystyrene (SBS) block copolymer. These functionalized SBS copolymers were evaluated for biodegradation using the fungal culture Aspergillus niger NCIM 1025 (ATCC 9642) and bacterial culture Pseudomonas sp. NCIM 2220, and for antimicrobial properties using bacteria E. coli DH5α and Bacillus subtilis and yeasts Pichia stipitis NCIM 3497 and P. stipitis NCIM 3499. It was conclusively demonstrated that these modified SBS block copolymers were significantly more biodegradable than the unmodified SBS;the observed weight loss after biodegradation was ~4 - 14-fold for bacterial and ~7 - 36-fold for fungal cultures with respect to the sugar content of modified SBS. Preliminary studies on antimicrobial properties of these biodegradable polymers showed a 4% - 24% decrease in growth of the microorganisms E. coli and Bacillus subtilis studied.

关键词： Biodegradation Antimicrobial SBS Sugar Quaternary Nitrogen

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