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Diagnostic assays developed for the control of foot-andmouth disease in India

World Journal of Virology 2015年第3期4卷 295-302页

作者： Gaurav Kumar Sharma Sonalika Mahajan rakesh matura Saravanan Subramaniam Rajeev Ranjan Jitendra Biswal Manoranjan Rout Jajati Keshari Mohapatra Bana Bihari Dash Aniket Sanyal Bramhadev Pattnaik Project Directorate on Foot and Mouth Disease Indian Council of Agricultural Research

Foot-and-mouth disease(FMD) is a highly contagious and economically devastating disease of livestock, primarily affecting cattle, buffalo and pigs. FMD virus serotypes O, A and Asia1 are prevalent in India and systematic efforts are on to control and eventually eradicate the disease from the country. FMD epidemiology is complex due to factors like co-circulation, extinction, emergence and re-emergence of genotypes/lineages within the three serotypes, animal movement, diverse farm practices and large number of susceptible livestock in the country. Systematic vaccination, prompt diagnosis, strict biosecurity measures, and regular monitoring of vaccinal immunity and surveillance of virus circulation are indispensible features for the effective implementation of the control measures. Availability of suitable companion diagnostic tests is very important in this endeavour. In this review, the diagnostic assays developed and validated in India and their contribution in FMD control programme is presented.

关键词： Foot-and-mouth disease Diagnosis Serosurveillance Sero-monitoring Multiplex polymerase chain reaction Real-time polymerase chain reaction Lineage differentiating polymerase chain reaction

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空气氧化的乙氧基化物表面活性剂多中心斑帖试验研究

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世界核心医学期刊文摘（皮肤病学分册） 2005年第4期1卷 25-26页

作者： matura M. Bodin A. Skare L. 田中伟 Dermatochemistry and Skin Allergy Department of Chemistry Gteborg University SE-41296 Gothenburg Sweden

Frequent exposure to water and surfactants is considered to be the main cause of hand eczema from wet work. Ethoxylated surfactants are susceptible to oxidation and some of the oxidation products formed have proved to be contact sensitizers in guinea pigs. The question of human sensitization to oxidized surfactants was addressed in a multicentre study in the Stockholm region. 528 consecutive dermatitis patients were patch tested with widely used ethoxylated surfactants in oxidized and non-oxidized form as well as certain identified oxidation compounds. 61 patients presented with mild, clearly irritant reactions to some of the surfactants tested. 18 patients showed not only erythema but also oedema and/or papules and vesicles, using a morphologic descriptive system for reading the patch test reactions. These reactions occurred mostly to oxidized surfactants and oxidation products. When retesting 9 of these 18 patients only an allergic reaction to acetaldehyde was confirmed. We conclude that oxidized ethoxylated surfactants have increased irritant potential compared to non-oxidized material. Our working hypothesis is that oxidized surfactants of technical quality exert a lower risk of sensitization than do oxidized homologous pure surfactants. Among the potential allergens formed during autoxidation, formaldehyde and acetaldehyde must be considered as a source of unexpected exposure.

关键词：乙氧基化表面活性物质空气氧化手部湿疹试验研究斑贴试验刺激反应性过敏氧化物质工作环境

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选择性氧化芳香族萜烯是普遍存在的接触性变应原

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世界核心医学期刊文摘（皮肤病学分册） 2005年第9期1卷 45-46页

作者： matura M. Skrje A.etal. old M. B 惠海英 Dermatochemistry and Skin Allergy Department of Chemistry Gteborg University SE-41296GothenburgSweden

Terpenes are widely used fragrance compounds in fine fragrances, but also in domestic and occupational products. Terpenes oxidize easily due to autoxidation on air exposure. Previous studies have shown that limonene, linalool and caryophyllene are not allergenic themselves but readily form allergenic products on air-exposure. This study aimed to determine the frequency and characteristics of allergic reactions to selected oxidized fragrance terpenes other than limonene. In total 1511 consecutive dermatitis patients in 6 European dermatology centres were patch tested with oxidized fragrance terpenes and some oxidation fractions and compounds. Oxidized linalool and its hydroperoxide fraction were found to be common contact allergens. Of the patients tested, 1.3%showed a positive reaction to oxidized linalool and 1.1%to the hydroperoxide fraction. About 0.5%of the patients reacted to oxidized caryophyllene whereas 1 patient reacted to oxidized myrcene. Of the patients reacting to the oxidized terpenes, 58%had fragrance-related contact allergy and/or a positive history for adverse reaction to fragrances. Autoxidation of fragrance terpenes contributes greatly to fragrance allergy, which emphasizes the need of testing with compounds that patients are actually exposed to and not only with the ingredients originally applied in commercial formulations.

关键词：接触性变应原萜烯选择性氧化接触性变态反应斑贴试验氧化芳樟醇丁香烯自氧化变应性反应柠檬烯

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